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TMEM196 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol TMEM196
Entrez Gene ID 256130
Full Name transmembrane protein 196
General protein information
Preferred Names

transmembrane protein 196

Names

transmembrane protein 196
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

7

7p21.1

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_152774.3 NP_689987.3 transmembrane protein 196 isoform 1
XM_017011929.1 XP_016867418.1 transmembrane protein 196 isoform X3
XM_011515249.2 XP_011513551.1 transmembrane protein 196 isoform X4
XM_017011928.1 XP_016867417.1 transmembrane protein 196 isoform X1
NM_001366627.1 NP_001353556.1 transmembrane protein 196 isoform 5
XM_005249692.4 XP_005249749.2 transmembrane protein 196 isoform X2
XM_011515250.2 XP_011513552.1 transmembrane protein 196 isoform X5
NM_001366628.1 NP_001353557.1 transmembrane protein 196 isoform 6
XM_017011928.2 XP_016867417.1 transmembrane protein 196 isoform X1
NM_001366626.1 NP_001353555.1 transmembrane protein 196 isoform 4
XM_017011929.2 XP_016867418.1 transmembrane protein 196 isoform X3
NM_001363562.1 NP_001350491.1 transmembrane protein 196 isoform 2
NM_001366625.1 NP_001353554.1 transmembrane protein 196 isoform 3
NM_001363562.2 NP_001350491.1 transmembrane protein 196 isoform 2

Pathways from BioSystems


Homologies


Xenopus tropicalis (tropical clawed frog) tmem196 NP_001096449.1
Pan troglodytes (chimpanzee) TMEM196 XP_001151953.2
Rattus norvegicus (Norway rat) Tmem196 NP_001037734.1
Bos taurus (cattle) TMEM196 NP_001193700.1
Danio rerio (zebrafish) LOC798466 XP_001338909.3
Homo sapiens (human) TMEM196 NP_689987.3
Canis lupus familiaris (dog) TMEM196 XP_003431964.1
Gallus gallus (chicken) TMEM196 XP_004939398.1
TMEM196 XP_001102739.2
Mus musculus (house mouse) Tmem196 NP_001153857.1

Gene Ontology


Bibliography

Related articles in PubMed

TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is a potential prognostic biomarker in lung cancer.
Liu WB, Han F, Jiang X, Chen HQ, Zhao H, Liu Y, Li YH, Huang C, Cao J, Liu JY
Oncotarget6(25)21225-39(2015 Aug)

Genome-wide association study of intracranial aneurysm identifies a new association on chromosome 7.
Foroud T, Lai D, Koller D, Van't Hof F, Kurki MI, Anderson CS, Brown RD Jr, Connolly ES, Eriksson JG, Flaherty M, Fornage M, von Und Zu Fraunberg M, Ga?l EI, Laakso A, Hernesniemi J, Huston J, J??skel?inen JE, Kiemeney LA, Kivisaari R, Kleindorfer D, Ko N, Lehto H, Mackey J, Meissner I, Moomaw CJ, Mosley TH, Moskala M, Niemel? M, Palotie A, Pera J, Rinkel G, Ripke S, Rouleau G, Ruigrok Y, Sauerbeck L, S?owik A, Vermeulen SH, Woo D, Worrall BB, Broderick J,
Stroke45(11)3194-9(2014 Nov)

A genome-wide association for kidney function and endocrine-related traits in the NHLBI's Framingham Heart Study.
Hwang SJ, Yang Q, Meigs JB, Pearce EN, Fox CS
BMC medical genetics8 Suppl 1S10(2007 Sep)

Creation of genome-wide protein expression libraries using random activation of gene expression.
Harrington JJ, Sherf B, Rundlett S, Jackson PD, Perry R, Cain S, Leventhal C, Thornton M, Ramachandran R, Whittington J, Lerner L, Costanzo D, McElligott K, Boozer S, Mays R, Smith E, Veloso N, Klika A, Hess J, Cothren K, Lo K, Offenbacher J, Danzig J, Ducar M
Nature biotechnology19(5)440-5(2001 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is an independent prognostic factor of lung cancer
Title: TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is a potential prognostic biomarker in lung cancer.

The following TMEM196 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TMEM196 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu06920 NM_152774.3
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK		 $49.50-$69.30
$99.00
OHu78361 NM_001366625.1
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu78363 NM_001366626.1
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu78361 XM_005249692.4
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu78362 XM_011515249.2
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu78363 XM_011515250.2
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
OHu107039 NM_001366628.1
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OHu107040 NM_001366627.1
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OHu78362 NM_001363562.2
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OHu97136 XM_017011928.2
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OHu97137 XM_017011929.2
Latest version!		 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
View Old Accession Versions >>
OHu97136 XM_017011928.1 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OHu97137 XM_017011929.1 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $69.30
$99.00
OHu78362 NM_001363562.1 Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu06920
Clone ID Related Accession (Same CDS sequence) NM_152774.3
Accession Version NM_152774.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 519bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-09
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX648384.1, DN991960.1 and BX647256.1. On Aug 29, 2008 this sequence version replaced NM_152774.2. ##Evidence-Data-START## Transcript exon combination :: BX648384.1, SRR1660803.15192.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142348, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAG AAATAACAGA CAACATGAGC 
AATGGAGGAC CACAGCTGAT ATTTAATGGA AGAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_689987.3
CDS86..604
Translation

Target ORF information:

RefSeq Version NM_152774.3
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_152774.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAG AAATAACAGA CAACATGAGC 
AATGGAGGAC CACAGCTGAT ATTTAATGGA AGAGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78361
Clone ID Related Accession (Same CDS sequence) XM_005249692.4 , NM_001366625.1
Accession Version NM_001366625.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 558bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-09
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform 3
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004543.2, BG197605.1, BX648384.1 and BX647256.1. On Oct 13, 2018 this sequence version replaced XM_005249692.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.265042.1, SRR1660809.11077.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001353554.1
CDS922..1479
Translation

Target ORF information:

RefSeq Version NM_001366625.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001366625.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78362
Clone ID Related Accession (Same CDS sequence) XM_011515249.2 , NM_001363562.1 , NM_001363562.2
Accession Version NM_001363562.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-04-30
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004543.2. On May 24, 2018 this sequence version replaced XM_011515249.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.229817.1, SRR1660803.39759.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
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ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001350491.1
CDS710..1249
Translation

Target ORF information:

RefSeq Version NM_001363562.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001363562.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78363
Clone ID Related Accession (Same CDS sequence) XM_011515250.2 , NM_001366626.1
Accession Version NM_001366626.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-10-22
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform 4
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004543.2 and CK823401.1. On Oct 13, 2018 this sequence version replaced XM_011515250.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.187064.1, SRR1660805.187599.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142586, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
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241
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421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAGAA 
ATAACAGACA ACATGAGCAA TGGAGGACCA CAGCTGATAT TTAATGGAAG AGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001353555.1
CDS922..1458
Translation

Target ORF information:

RefSeq Version NM_001366626.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001366626.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAGAA 
ATAACAGACA ACATGAGCAA TGGAGGACCA CAGCTGATAT TTAATGGAAG AGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78361
Clone ID Related Accession (Same CDS sequence) XM_005249692.4 , NM_001366625.1
Accession Version XM_005249692.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 558bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249692.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
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541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005249749.2
CDS1005..1562
Translation

Target ORF information:

RefSeq Version XM_005249692.4
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005249692.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78362
Clone ID Related Accession (Same CDS sequence) XM_011515249.2 , NM_001363562.1 , NM_001363562.2
Accession Version XM_011515249.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515249.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513551.1
CDS1005..1544
Translation

Target ORF information:

RefSeq Version XM_011515249.2
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011515249.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78363
Clone ID Related Accession (Same CDS sequence) XM_011515250.2 , NM_001366626.1
Accession Version XM_011515250.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 537bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform X5
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011515250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAGAA 
ATAACAGACA ACATGAGCAA TGGAGGACCA CAGCTGATAT TTAATGGAAG AGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011513552.1
CDS1005..1541
Translation

Target ORF information:

RefSeq Version XM_011515250.2
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011515250.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAGAA 
ATAACAGACA ACATGAGCAA TGGAGGACCA CAGCTGATAT TTAATGGAAG AGTGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu107039
Clone ID Related Accession (Same CDS sequence) NM_001366628.1
Accession Version NM_001366628.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 315bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-09
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform 6
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004543.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.192894.1, SRR1660807.132743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGATCCTCT TTTCAGCCTG CTGTATCTGT GGACTTATTG GGGGCATCCT GAATTTTCAG 
TTCCTCCGGG CAGTCACAAA GAAAACTTCC TCCCTATACC CACTGCACCT TGCCTCCATG 
TCTCTCGCGT GCATTGGGAT CGGGGGCTGC ACTCTCTCTT CCTGGCTCAC TTGTCGACTA 
GCCAGTTATG AACAGAGGAG GATGTTCTCA GAAAGGGAGC ATTCCCTGCA TCACTCTCAT 
GAAATGGCTG AGAAAGAAAT AACAGACAAC ATGAGCAATG GAGGACCACA GCTGATATTT 
AATGGAAGAG TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001353557.1
CDS109..423
Translation

Target ORF information:

RefSeq Version NM_001366628.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001366628.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGATCCTCT TTTCAGCCTG CTGTATCTGT GGACTTATTG GGGGCATCCT GAATTTTCAG 
TTCCTCCGGG CAGTCACAAA GAAAACTTCC TCCCTATACC CACTGCACCT TGCCTCCATG 
TCTCTCGCGT GCATTGGGAT CGGGGGCTGC ACTCTCTCTT CCTGGCTCAC TTGTCGACTA 
GCCAGTTATG AACAGAGGAG GATGTTCTCA GAAAGGGAGC ATTCCCTGCA TCACTCTCAT 
GAAATGGCTG AGAAAGAAAT AACAGACAAC ATGAGCAATG GAGGACCACA GCTGATATTT 
AATGGAAGAG TGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu107040
Clone ID Related Accession (Same CDS sequence) NM_001366627.1
Accession Version NM_001366627.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 336bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-07-09
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform 5
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004543.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.321902.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
ATGATCCTCT TTTCAGCCTG CTGTATCTGT GGACTTATTG GGGGCATCCT GAATTTTCAG 
TTCCTCCGGG CAGTCACAAA GAAAACTTCC TCCCTATACC CACTGCACCT TGCCTCCATG 
TCTCTCGCGT GCATTGGGAT CGGGGGCTGC ACTCTCTCTT CCTGGCTCAC TTGTCGACTA 
GCCAGTTATG AACAGAGGAG GATGTTCTCA GAAAGGGAGC ATTCCCTGCA TCACTCTCAT 
GAAATGGCTG AGAAAAGATT GAGGGCTATT GAAATAACCG ACTTGCCCAG CTGCCCGGTG 
GTGCCCCCGA CACCAGAGTT ACCTACAAGG AAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001353556.1
CDS109..444
Translation

Target ORF information:

RefSeq Version NM_001366627.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001366627.1

ORF Insert Sequence:

1
61
121
181
241
301
ATGATCCTCT TTTCAGCCTG CTGTATCTGT GGACTTATTG GGGGCATCCT GAATTTTCAG 
TTCCTCCGGG CAGTCACAAA GAAAACTTCC TCCCTATACC CACTGCACCT TGCCTCCATG 
TCTCTCGCGT GCATTGGGAT CGGGGGCTGC ACTCTCTCTT CCTGGCTCAC TTGTCGACTA 
GCCAGTTATG AACAGAGGAG GATGTTCTCA GAAAGGGAGC ATTCCCTGCA TCACTCTCAT 
GAAATGGCTG AGAAAAGATT GAGGGCTATT GAAATAACCG ACTTGCCCAG CTGCCCGGTG 
GTGCCCCCGA CACCAGAGTT ACCTACAAGG AAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu78362
Clone ID Related Accession (Same CDS sequence) XM_011515249.2 , NM_001363562.1 , NM_001363562.2
Accession Version NM_001363562.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 540bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-30
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC004543.2. On Aug 31, 2019 this sequence version replaced NM_001363562.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.229817.1, SRR1660803.39759.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001350491.1
CDS922..1461
Translation

Target ORF information:

RefSeq Version NM_001363562.2
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001363562.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGAAATAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97136
Clone ID Related Accession (Same CDS sequence) XM_017011928.1 , XM_017011928.2
Accession Version XM_017011928.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 567bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGTACGCTGA TTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867417.1
CDS1005..1571
Translation

Target ORF information:

RefSeq Version XM_017011928.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011928.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGTACGCTGA TTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97136
Clone ID Related Accession (Same CDS sequence) XM_017011928.1 , XM_017011928.2
Accession Version XM_017011928.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 567bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGTACGCTGA TTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867417.1
CDS1736..2302
Translation

Target ORF information:

RefSeq Version XM_017011928.2
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011928.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGGTA TGGAGCGGGG TGTGTTTTCT TCTTTGTGGC 
ATTTGTGGAA TATTGTGTGC CAAAAAAAAA TCAGGACTTG TCATGATCCT CTTTTCAGCC 
TGCTGTATCT GTGGACTTAT TGGGGGCATC CTGAATTTTC AGTTCCTCCG GGCAGTCACA 
AAGAAAACTT CCTCCCTATA CCCACTGCAC CTTGCCTCCA TGTCTCTCGC GTGCATTGGG 
ATCGGGGGCT GCACTCTCTC TTCCTGGCTC ACTTGTCGAC TAGCCAGTTA TGAACAGAGG 
AGGATGTTCT CAGAAAGGGA GCATTCCCTG CATCACTCTC ATGAAATGGC TGAGAAAAGA 
TTGAGGGCTA TTGAAATAAC CGACTTGCCC AGCTGCCCGG TGGTGCCCCC GACACCAGAG 
TTACCTACAA GGTACGCTGA TTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97137
Clone ID Related Accession (Same CDS sequence) XM_017011929.1 , XM_017011929.2
Accession Version XM_017011929.1 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 549bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGTACGCT 
GATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867418.1
CDS1005..1553
Translation

Target ORF information:

RefSeq Version XM_017011929.1
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011929.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGTACGCT 
GATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu97137
Clone ID Related Accession (Same CDS sequence) XM_017011929.1 , XM_017011929.2
Accession Version XM_017011929.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 549bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product transmembrane protein 196 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000007.14) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017011929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGTACGCT 
GATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016867418.1
CDS1736..2284
Translation

Target ORF information:

RefSeq Version XM_017011929.2
Organism Homo sapiens(human)
Definition Homo sapiens transmembrane protein 196 (TMEM196), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_017011929.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
ATGTGCACCA GCGGTCAGAT TATTGGGAGC CTCTTGGTGC TCTCCGTGCT GGAGATAGGG 
CTGGGGGTGT CCAGCGTGGC CGTGGGGGCG GTCAGCTTCA GCCTGGCCCT CCGAGAGCAC 
AAGCCGCAGC TCGGAGACTC GTCCCCGTTT CTTCTTTGTG GCATTTGTGG AATATTGTGT 
GCCAAAAAAA AATCAGGACT TGTCATGATC CTCTTTTCAG CCTGCTGTAT CTGTGGACTT 
ATTGGGGGCA TCCTGAATTT TCAGTTCCTC CGGGCAGTCA CAAAGAAAAC TTCCTCCCTA 
TACCCACTGC ACCTTGCCTC CATGTCTCTC GCGTGCATTG GGATCGGGGG CTGCACTCTC 
TCTTCCTGGC TCACTTGTCG ACTAGCCAGT TATGAACAGA GGAGGATGTT CTCAGAAAGG 
GAGCATTCCC TGCATCACTC TCATGAAATG GCTGAGAAAA GATTGAGGGC TATTGAAATA 
ACCGACTTGC CCAGCTGCCC GGTGGTGCCC CCGACACCAG AGTTACCTAC AAGGTACGCT 
GATTTCTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

TMEM196 acts as a novel functional tumour suppressor inactivated by DNA methylation and is a potential prognostic biomarker in lung cancer.
Oncotarget6(25)21225-39(2015 Aug)
Liu WB,Han F,Jiang X,Chen HQ,Zhao H,Liu Y,Li YH,Huang C,Cao J,Liu JY


book

Genome-wide association study of intracranial aneurysm identifies a new association on chromosome 7.
Stroke45(11)3194-9(2014 Nov)
Foroud T,Lai D,Koller D,Van't Hof F,Kurki MI,Anderson CS,Brown RD Jr,Connolly ES,Eriksson JG,Flaherty M,Fornage M,von Und Zu Fraunberg M,Ga?l EI,Laakso A,Hernesniemi J,Huston J,J??skel?inen JE,Kiemeney LA,Kivisaari R,Kleindorfer D,Ko N,Lehto H,Mackey J,Meissner I,Moomaw CJ,Mosley TH,Moskala M,Niemel? M,Palotie A,Pera J,Rinkel G,Ripke S,Rouleau G,Ruigrok Y,Sauerbeck L,S?owik A,Vermeulen SH,Woo D,Worrall BB,Broderick J,


book

A genome-wide association for kidney function and endocrine-related traits in the NHLBI's Framingham Heart Study.
BMC medical genetics8 Suppl 1S10(2007 Sep)
Hwang SJ,Yang Q,Meigs JB,Pearce EN,Fox CS


book

Creation of genome-wide protein expression libraries using random activation of gene expression.
Nature biotechnology19(5)440-5(2001 May)
Harrington JJ,Sherf B,Rundlett S,Jackson PD,Perry R,Cain S,Leventhal C,Thornton M,Ramachandran R,Whittington J,Lerner L,Costanzo D,McElligott K,Boozer S,Mays R,Smith E,Veloso N,Klika A,Hess J,Cothren K,Lo K,Offenbacher J,Danzig J,Ducar M