PCF11 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCF11
Entrez Gene ID	51585
Full Name	PCF11 cleavage and polyadenylation factor subunit
General protein information	Preferred Names PCF11 cleavage and polyadenylation factor subunit Names pre-mRNA cleavage complex 2 protein Pcf11 PCF11, cleavage and polyadenylation factor subunit, homolog PCF11p homolog pre-mRNA cleavage complex II protein Pcf11
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q14.1
Summary	The protein encoded by this gene binds to CLP1 to form pre-mRNA cleavage factor IIm. The encoded protein is necessary for efficient Pol II transcription termination and may be involved in degradation of the 3' product of polyA site cleavage. [provided by RefSeq, Oct 2016].
Disorder MIM:	608876

mRNA and Protein(s)

mRNA	Protein	Name
NM_001346413.2	NP_001333342.1	pre-mRNA cleavage complex 2 protein Pcf11 isoform 1
NM_015885.3	NP_056969.2	pre-mRNA cleavage complex 2 protein Pcf11 isoform 3
NM_001346415.1	NP_001333344.1	pre-mRNA cleavage complex 2 protein Pcf11 isoform 4
NM_001346413.1	NP_001333342.1	pre-mRNA cleavage complex 2 protein Pcf11 isoform 1
NM_001346415.2	NP_001333344.1	pre-mRNA cleavage complex 2 protein Pcf11 isoform 4
NM_001346414.2	NP_001333343.1	pre-mRNA cleavage complex 2 protein Pcf11 isoform 2
NM_001346414.1	NP_001333343.1	pre-mRNA cleavage complex 2 protein Pcf11 isoform 2
NM_015885.4	NP_056969.2	pre-mRNA cleavage complex 2 protein Pcf11 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PCF11	XP_508673.4
	PCF11	XP_002808130.1
Gallus gallus (chicken)	PCF11	XP_417214.3
Danio rerio (zebrafish)	pcf11	NP_001007308.1
Homo sapiens (human)	PCF11	NP_056969.2
Bos taurus (cattle)	LOC510604	XP_002699113.2
Canis lupus familiaris (dog)	PCF11	XP_533992.4
Mus musculus (house mouse)	Pcf11	NP_083354.3
Rattus norvegicus (Norway rat)	Pcf11	XP_341884.4

Gene Ontology
Bibliography

Related articles in PubMed

WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription.
Volanakis A, Kamieniarz-Gdula K, Schlackow M, Proudfoot NJ
Genes & development31(21)2175-2185(2017 Nov)

Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination.
West S, Proudfoot NJ
Nucleic acids research36(3)905-14(2008 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

WNK1 and the associated phosphorylation of the PCF11 CID act to promote transcript release from chromatin-associated Pol II, which in turn facilitates mRNA export to the cytoplasm
Title: WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription.

Pcf11 is required for the efficient degradation of the 3' product of poly(A) site cleavage.
Title: Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination.

Pcf11 can act as a negative elongation factor to repress RNA Pol II gene expression in eukaryotic cells.
Title: Transcription termination factor Pcf11 limits the processivity of Pol II on an HIV provirus to repress gene expression.

Human PCf11 is a pre-mRNA processing factor.
Title: Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors.

The following PCF11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCF11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29064	NM_015885.4 Latest version!	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu100712	NM_001346415.2 Latest version!	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu45367	NM_001346413.2 Latest version!	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu45368	NM_001346414.2 Latest version!	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu45367	NM_001346413.1	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu100712	NM_001346415.1	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu45368	NM_001346414.1	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu29064	NM_015885.3	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCF11 ( NM_015885.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_056969.2 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.

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CloneID	OHu29064
Clone ID Related Accession (Same CDS sequence)	NM_015885.3 , NM_015885.4
Accession Version	NM_015885.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB053362.1 and BC146778.1. This sequence is a reference standard in the RefSeqGene project. On Sep 22, 2007 this sequence version replaced NM_015885.2. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC146778.1, SRR1803615.79390.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG 
CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT 
TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT 
ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT 
CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT 
CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG 
GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT 
CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT 
GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA 
TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA 
GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA 
AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT 
CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA 
ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056969.2
CDS	346..5013
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_015885.3
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015885.3

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG 
CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT 
TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT 
ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT 
CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT 
CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG 
GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT 
CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT 
GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA 
TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA 
GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA 
AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT 
CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA 
ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( NM_015885.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_056969.2 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu29064
Clone ID Related Accession (Same CDS sequence)	NM_015885.3 , NM_015885.4
Accession Version	NM_015885.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB053362.1, BC146778.1 and AP000873.5. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_015885.3. Transcript Variant: This variant (3) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC146778.1, AB020631.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG 
CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT 
TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT 
ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT 
CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT 
CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG 
GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT 
CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT 
GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA 
TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA 
GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA 
AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT 
CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA 
ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_056969.2
CDS	310..4977
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_015885.4
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015885.4

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACAAG TAAGTGAAGT AACTGCTCAG 
CCTCCCCCTG AAGAGGAGGA AGATCAAAAT GAAGATCAAG ATGTTCCAGA TCTTACTAAT 
TTTACAGTTG AAGAATTGAA ACAACGTTAT GACAGTGTTA TAAATCGACT GTACACTGGT 
ATTCAGTGTT ACTCTTGTGG AATGAGGTTT ACAACATCAC AGACAGATGT TTATGCAGAT 
CATTTGGACT GGCATTATCG GCAAAATAGA ACTGAAAAGG ATGTTAGCCG AAAAGTCACT 
CATAGACGTT GGTACTACAG TTTAACAGAC TGGATAGAAT TTGAGGAGAT AGCTGATCTG 
GAAGAACGGG CAAAGAGCCA GTTTTTTGAA AAGGTGCATG AAGAAGTTGT GCTCAAAACT 
CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA AGTGTACCTG CTGGACCAGC TGGAGCAGTT 
GAGAGTTGTG AAATCTGTCA AGAACAATTT GAACAATACT GGGATGAAGA AGAGGAGGAA 
TGGCATTTGA AAAATGCTAT TAGAGTAGAT GGAAAGATTT ATCATCCATC ATGTTATGAA 
GATTATCAAA ATACATCTTC ATTTGATTGT ACACCATCTC CCAGCAAGAC ACCAGTTGAA 
AACCCCTTGA ATATTATGTT GAACATTGTC AAAAACGAAT TGCAGGAACC CTGTGACAGT 
CCCAAAGTTA AGGAAGAACG AATTGATACA CCACCAGCTT GTACAGAGGA AAGCATAGCA 
ACACCCTCTG AAATTAAAAC AGAAAATGAC ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( NM_001346415.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333344.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu100712
Clone ID Related Accession (Same CDS sequence)	NM_001346415.1 , NM_001346415.2
Accession Version	NM_001346415.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Oct 9, 2016 this sequence version replaced XM_017017918.1. Transcript Variant: This variant (4) uses two alternate in-frame splice junctions compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.233378.1, SRR1803611.192824.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT 
CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT 
ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT 
CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT 
TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT 
AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA 
GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA 
GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG 
AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG 
CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT 
TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC 
CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC 
AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA 
CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333344.1
CDS	346..5010
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTLSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_001346415.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346415.1

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT 
CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT 
ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT 
CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT 
TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT 
AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA 
GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA 
GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG 
AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG 
CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT 
TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC 
CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC 
AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA 
CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( NM_001346415.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333344.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.

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CloneID	OHu100712
Clone ID Related Accession (Same CDS sequence)	NM_001346415.1 , NM_001346415.2
Accession Version	NM_001346415.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Jun 2, 2019 this sequence version replaced NM_001346415.1. Transcript Variant: This variant (4) uses two alternate in-frame splice junctions compared to variant 1. The resulting isoform (4) has the same N- and C-termini but is shorter compared to variant 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.233378.1, SRR1803611.192824.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT 
CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT 
ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT 
CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT 
TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT 
AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA 
GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA 
GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG 
AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG 
CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT 
TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC 
CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC 
AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA 
CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333344.1
CDS	310..4974
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTLSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_001346415.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346415.2

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTGTGCGA 
GAAGAGCAGA GATCTCCATT CAATGATCGT TTTCCACTTA AGCGACCTCG ATATGAAGAT 
TCAGATAAAC CATTTGTAGA TAGTCCAGCA TCAAGATTCG CCGGCCTGGA TACAAATCAG 
CGACTTACAG CTTTAGCTGA AGACAGACCG TTATTTGATG GACCTAGTAG GCCATCAGTA 
GCAAGAGATG GCCCAACGAA GATGATTTTT GAAGGACCCA ATAAATTAAG CCCTCGAATT 
GATGGACCTC CCACACCAGC TTCTCTTCGG TTTGATGGGT CACCAGGACA AATGGGGGGA 
GGAGGCCCTT TGAGATTTGA GGGGCCACAA GGTCAGCTAG GAGGTGGGTG TCCTTTGAGA 
TTTGAAGGTC CTCCAGGACC AGTGGGGACA CCTCTGCGGT TTGAGGGCCC AATTGGTCAA 
GCAGGAGGAG GTGGTTTTCG GTTTGAAGGT TCCCCTGGTC TGAGGTTTGA GGGATCTCCA 
GGTGGTTTGA GATTTGAGGG ACCAGGAGGC CAGCCTGTGG GTGGTCTGAG GTTTGAGGGA 
CATCGTGGTC AACCTGTGGG TGGTCTAAGG TTTGAGGGAC CTCATGGTCA GCCTGTGGGT 
GGACTTAGAT TTGATAATCC CCGAGGTCAG CCTGTAGGTG GACTTAGATT TGAGGGGGGT 
CATGGTCCAT CAGGGGCTGC GATTAGGTTT GATGGACCTC ATGGTCAGCC AGGAGGTGGA 
ATCAGATTTG AGGGCCCTTT GCTACAGCAA GGGGTTGGAA TGAGGTTTGA GGGCCCCCAT 
GGTCAGTCAG TAGCTGGTCT GAGATTTGAG GGACAACATA ATCAACTTGG TGGGAACCTT 
AGGTTTGAGG GTCCACATGG TCAGCCAGGG GTTGGTATCA GGTTTGAAGG CCCTTTAGTC 
CAACAAGGAG GTGGAATGAG GTTTGAGGGT CCTTCTGTAC CAGGAGGTGG CCTGAGAATT 
GAAGGGCCTC TGGGTCAAGG TGGTCCAAGA TTTGAAGGTT GTCATGCTTT AAGGTTTGAT 
GGGCAGCCAG GTCAGCCGTC ACTCTTGCCA AGATTTGATG GATTACATGG TCAGCCAGGT 
CCTAGATTTG AAAGGACTCC TGGTCAGCCA GGCCCTCAGA GGTTTGATGG ACCACCTGGA 
CAGCAGGTTC AACCCAGATT TGACGGTGTA CCTCAAAGAT TTGATGGTCC ACAACATCAG 
CAAGCATCAA GGTTTGATAT TCCTCTTGGT CTTCAAGGCA CAAGATTTGA CAATCATCCT 
TCACAAAGGC TTGAATCAGT ATCTTTCAAT CAGACTGGTC CATATAATGA TCCACCTGGC 
AATGCTTTTA ATGCCCCATC CCAAGGACTA CAGTTCCAAA GACATGAACA AATATTTGAT 
TCACCTCAAG GACCAAATTT TAATGGACCA CATGGCCCTG GAAACCAGAG TTTCTCTAAT 
CCACTTAACA GAGCTTCTGG ACACTATTTT GATGAAAAAA ATCTTCAGAG TTCTCAATTT 
GGAAACTTTG GCAATATACC TGCTCCAATG ACAGTAGGAA ATATTCAGGC ATCTCAACAG 
GTTCTGAGTG GTGTTGCTCA GCCAGTAGCT TTTGGTCAAG GACAACAGTT TTTACCAGTT 
CATCCACAAA ATCCTGGATT TGTTCAGAAT CCTTCAGGAG CCCTCCCTAA GGCATATCCT 
GATAATCATC TCAGTCAGGT GGATGTAAAT GAATTGTTTT CAAAATTGCT AAAAACAGGA 
ATTCTCAAAT TGTCCCAAAC TGATTCAGCT ACAACACTAA GTGAAGTAAC TGCTCAGCCT 
CCCCCTGAAG AGGAGGAAGA TCAAAATGAA GATCAAGATG TTCCAGATCT TACTAATTTT 
ACAGTTGAAG AATTGAAACA ACGTTATGAC AGTGTTATAA ATCGACTGTA CACTGGTATT 
CAGTGTTACT CTTGTGGAAT GAGGTTTACA ACATCACAGA CAGATGTTTA TGCAGATCAT 
TTGGACTGGC ATTATCGGCA AAATAGAACT GAAAAGGATG TTAGCCGAAA AGTCACTCAT 
AGACGTTGGT ACTACAGTTT AACAGACTGG ATAGAATTTG AGGAGATAGC TGATCTGGAA 
GAACGGGCAA AGAGCCAGTT TTTTGAAAAG GTGCATGAAG AAGTTGTGCT CAAAACTCAA 
GAGGCTGCTA AAGAAAAAGA GTTCCAAAGT GTACCTGCTG GACCAGCTGG AGCAGTTGAG 
AGTTGTGAAA TCTGTCAAGA ACAATTTGAA CAATACTGGG ATGAAGAAGA GGAGGAATGG 
CATTTGAAAA ATGCTATTAG AGTAGATGGA AAGATTTATC ATCCATCATG TTATGAAGAT 
TATCAAAATA CATCTTCATT TGATTGTACA CCATCTCCCA GCAAGACACC AGTTGAAAAC 
CCCTTGAATA TTATGTTGAA CATTGTCAAA AACGAATTGC AGGAACCCTG TGACAGTCCC 
AAAGTTAAGG AAGAACGAAT TGATACACCA CCAGCTTGTA CAGAGGAAAG CATAGCAACA 
CCCTCTGAAA TTAAAACAGA AAATGACACA GTCGAGTCAG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( NM_001346413.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333342.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.

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CloneID	OHu45367
Clone ID Related Accession (Same CDS sequence)	NM_001346413.2 , NM_001346413.1
Accession Version	NM_001346413.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Jun 2, 2019 this sequence version replaced NM_001346413.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.232134.1, SRR1803616.265971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA 
AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT 
TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG 
TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT 
AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA 
GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT 
GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC 
CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA 
TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA 
GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT 
TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT 
GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT 
ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT 
GACACAGTCG AGTCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333342.1
CDS	310..5370
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGKHRCNVRDSPTEENKGGLKKKPLLSDAELTYYEHKAKLKRTQVQHSFPRLDLLDPDIFDYPLTDALLSGIECEPSKSKHASRNSGAQFDRKEQFSERARRLSPISGSRTYAENLSPHEGRRRHDEQVSAKGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_001346413.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346413.2

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA 
AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT 
TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG 
TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT 
AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA 
GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT 
GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC 
CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA 
TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA 
GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT 
TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT 
GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT 
ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT 
GACACAGTCG AGTCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( NM_001346413.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333342.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.

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CloneID	OHu45367
Clone ID Related Accession (Same CDS sequence)	NM_001346413.2 , NM_001346413.1
Accession Version	NM_001346413.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Oct 8, 2016 this sequence version replaced XM_005274048.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.265971.1, SRR1803611.232134.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA 
AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT 
TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG 
TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT 
AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA 
GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT 
GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC 
CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA 
TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA 
GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT 
TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT 
GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT 
ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT 
GACACAGTCG AGTCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333342.1
CDS	346..5406
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGKHRCNVRDSPTEENKGGLKKKPLLSDAELTYYEHKAKLKRTQVQHSFPRLDLLDPDIFDYPLTDALLSGIECEPSKSKHASRNSGAQFDRKEQFSERARRLSPISGSRTYAENLSPHEGRRRHDEQVSAKGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTQVSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_001346413.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346413.1

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC AAGTAAGTGA AGTAACTGCT CAGCCTCCCC CTGAAGAGGA GGAAGATCAA 
AATGAAGATC AAGATGTTCC AGATCTTACT AATTTTACAG TTGAAGAATT GAAACAACGT 
TATGACAGTG TTATAAATCG ACTGTACACT GGTATTCAGT GTTACTCTTG TGGAATGAGG 
TTTACAACAT CACAGACAGA TGTTTATGCA GATCATTTGG ACTGGCATTA TCGGCAAAAT 
AGAACTGAAA AGGATGTTAG CCGAAAAGTC ACTCATAGAC GTTGGTACTA CAGTTTAACA 
GACTGGATAG AATTTGAGGA GATAGCTGAT CTGGAAGAAC GGGCAAAGAG CCAGTTTTTT 
GAAAAGGTGC ATGAAGAAGT TGTGCTCAAA ACTCAAGAGG CTGCTAAAGA AAAAGAGTTC 
CAAAGTGTAC CTGCTGGACC AGCTGGAGCA GTTGAGAGTT GTGAAATCTG TCAAGAACAA 
TTTGAACAAT ACTGGGATGA AGAAGAGGAG GAATGGCATT TGAAAAATGC TATTAGAGTA 
GATGGAAAGA TTTATCATCC ATCATGTTAT GAAGATTATC AAAATACATC TTCATTTGAT 
TGTACACCAT CTCCCAGCAA GACACCAGTT GAAAACCCCT TGAATATTAT GTTGAACATT 
GTCAAAAACG AATTGCAGGA ACCCTGTGAC AGTCCCAAAG TTAAGGAAGA ACGAATTGAT 
ACACCACCAG CTTGTACAGA GGAAAGCATA GCAACACCCT CTGAAATTAA AACAGAAAAT 
GACACAGTCG AGTCAGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( NM_001346414.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333343.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.

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CloneID	OHu45368
Clone ID Related Accession (Same CDS sequence)	NM_001346414.2 , NM_001346414.1
Accession Version	NM_001346414.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-22
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Jun 2, 2019 this sequence version replaced NM_001346414.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.23977.1, SRR1803616.226501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT 
GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT 
GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT 
ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA 
ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC 
TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA 
AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA 
AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT 
GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT 
GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT 
ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC 
AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA 
CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC 
ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333343.1
CDS	310..5367
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGKHRCNVRDSPTEENKGGLKKKPLLSDAELTYYEHKAKLKRTQVQHSFPRLDLLDPDIFDYPLTDALLSGIECEPSKSKHASRNSGAQFDRKEQFSERARRLSPISGSRTYAENLSPHEGRRRHDEQVSAKGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTLSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_001346414.2
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346414.2

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT 
GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT 
GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT 
ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA 
ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC 
TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA 
AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA 
AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT 
GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT 
GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT 
ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC 
AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA 
CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC 
ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCF11 ( NM_001346414.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001333343.1 Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu45368
Clone ID Related Accession (Same CDS sequence)	NM_001346414.2 , NM_001346414.1
Accession Version	NM_001346414.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-22
Organism	Homo sapiens(human)
Product	pre-mRNA cleavage complex 2 protein Pcf11 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000873.5. On Oct 9, 2016 this sequence version replaced XM_005274049.1. Transcript Variant: This variant (2) uses an alternate in-frame splice junction compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is 1 aa shorter compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.23977.1, SRR1803616.226501.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT 
GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT 
GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT 
ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA 
ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC 
TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA 
AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA 
AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT 
GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT 
GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT 
ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC 
AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA 
CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC 
ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333343.1
CDS	346..5403
Translation	MSEQTPAEAGAAGAREDACRDYQSSLEDLTFNSKPHINMLTILAEENLPFAKEIVSLIEAQTAKAPSSEKLPVMYLMDSIVKNVGREYLTAFTKNLVATFICVFEKVDENTRKSLFKLRSTWDEIFPLKKLYALDVRVNSLDPAWPIKPLPPNVNTSSIHVNPKFLNKSPEEPSTPGTVVSSPSISTPPIVPDIQKNLTQEQLIRQQLLAKQKQLLELQQKKLELELEQAKAQLAVSLSVQQETSNLGPGSAPSKLHVSQIPPMAVKAPHQVPVQSEKSRPGPSLQIQDLKGTNRDPRLNRISQHSHGKDQSHRKEFLMNTLNQSDTKTSKTIPSEKLNSSKQEKSKSGEKITKKELDQLDSKSKSKSKSPSPLKNKLSHTKDLKNQESESMRLSDMNKRDPRLKKHLQDKTDGKDDDVKEKRKTAEKKDKDEHMKSSEHRLAGSRNKIINGIVQKQDTITEESEKQGTKPGRSSTRKRSRSRSPKSRSPIIHSPKRRDRRSPKRRQRSMSPTSTPKAGKIRQSGAKQSHMEEFTPPSREDRNAKRSTKQDIRDPRRMKKTEEERPQETTNQHSTKSGTEPKENVENWQSSKSAKRWKSGWEENKSLQQVDEHSKPPHLRHRESWSSTKGILSPRAPKQQQHRLSVDANLQIPKELTLASKRELLQKTSERLASGEITQDDFLVVVHQIRQLFQYQEGKHRCNVRDSPTEENKGGLKKKPLLSDAELTYYEHKAKLKRTQVQHSFPRLDLLDPDIFDYPLTDALLSGIECEPSKSKHASRNSGAQFDRKEQFSERARRLSPISGSRTYAENLSPHEGRRRHDEQVSAKGVREEQRSPFNDRFPLKRPRYEDSDKPFVDSPASRFAGLDTNQRLTALAEDRPLFDGPSRPSVARDGPTKMIFEGPNKLSPRIDGPPTPASLRFDGSPGQMGGGGPLRFEGPQGQLGGGCPLRFEGPPGPVGTPLRFEGPIGQAGGGGFRFEGSPGLRFEGSPGGLRFEGPGGQPVGGLRFEGHRGQPVGGLRFEGPHGQPVGGLRFDNPRGQPVGGLRFEGGHGPSGAAIRFDGPHGQPGGGIRFEGPLLQQGVGMRFEGPHGQSVAGLRFEGQHNQLGGNLRFEGPHGQPGVGIRFEGPLVQQGGGMRFEGPSVPGGGLRIEGPLGQGGPRFEGCHALRFDGQPGQPSLLPRFDGLHGQPGPRFERTPGQPGPQRFDGPPGQQVQPRFDGVPQRFDGPQHQQASRFDIPLGLQGTRFDNHPSQRLESVSFNQTGPYNDPPGNAFNAPSQGLQFQRHEQIFDSPQGPNFNGPHGPGNQSFSNPLNRASGHYFDEKNLQSSQFGNFGNIPAPMTVGNIQASQQVLSGVAQPVAFGQGQQFLPVHPQNPGFVQNPSGALPKAYPDNHLSQVDVNELFSKLLKTGILKLSQTDSATTLSEVTAQPPPEEEEDQNEDQDVPDLTNFTVEELKQRYDSVINRLYTGIQCYSCGMRFTTSQTDVYADHLDWHYRQNRTEKDVSRKVTHRRWYYSLTDWIEFEEIADLEERAKSQFFEKVHEEVVLKTQEAAKEKEFQSVPAGPAGAVESCEICQEQFEQYWDEEEEEWHLKNAIRVDGKIYHPSCYEDYQNTSSFDCTPSPSKTPVENPLNIMLNIVKNELQEPCDSPKVKEERIDTPPACTEESIATPSEIKTENDTVESV

Target ORF information:

RefSeq Version	NM_001346414.1
Organism	Homo sapiens(human)
Definition	Homo sapiens PCF11 cleavage and polyadenylation factor subunit (PCF11), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346414.1

ORF Insert Sequence:

ATGTCAGAGC AGACGCCGGC CGAGGCCGGT GCTGCGGGGG CCCGGGAGGA CGCCTGTCGG 
GATTATCAGT CATCGCTCGA AGACCTGACC TTCAATAGCA AGCCGCACAT CAATATGCTG 
ACCATTCTAG CCGAGGAGAA CCTGCCCTTC GCCAAGGAGA TCGTCTCTCT CATCGAGGCC 
CAAACCGCCA AGGCTCCTTC CTCAGAGAAG CTTCCTGTTA TGTACCTTAT GGATTCTATT 
GTGAAAAACG TTGGAAGAGA GTATCTCACT GCCTTTACTA AAAATCTAGT TGCAACATTT 
ATTTGTGTGT TTGAAAAGGT GGATGAAAAT ACTAGGAAAA GTTTATTTAA GTTACGTTCT 
ACATGGGATG AAATATTCCC TTTGAAGAAA CTTTATGCCC TGGATGTCAG AGTCAATTCA 
TTAGATCCTG CTTGGCCTAT TAAACCTCTA CCCCCCAATG TGAATACGTC TAGCATCCAT 
GTGAATCCTA AATTTTTAAA TAAATCGCCC GAGGAGCCTT CAACACCTGG TACAGTGGTC 
AGTTCCCCTA GCATCTCCAC TCCTCCAATT GTTCCTGATA TACAAAAGAA TCTTACACAA 
GAACAACTAA TAAGGCAGCA GTTACTGGCA AAGCAAAAAC AGTTGTTAGA ACTTCAGCAG 
AAAAAGCTGG AGCTTGAGCT AGAGCAAGCT AAGGCACAGT TGGCAGTTTC TCTTAGTGTT 
CAGCAGGAAA CATCCAATTT AGGTCCTGGA TCTGCACCAT CCAAATTACA TGTTTCACAG 
ATTCCCCCTA TGGCAGTTAA AGCTCCTCAT CAGGTTCCTG TGCAATCTGA GAAAAGCCGT 
CCAGGACCAT CCTTACAAAT TCAGGATTTA AAAGGAACTA ACCGGGATCC TCGTCTGAAC 
AGGATAAGCC AACATTCTCA TGGAAAAGAT CAGAGTCACA GGAAAGAATT TCTAATGAAC 
ACATTAAACC AGTCTGATAC TAAGACAAGT AAAACTATAC CCTCTGAAAA ACTAAATTCA 
TCCAAGCAAG AAAAAAGTAA ATCAGGTGAA AAAATAACCA AGAAAGAACT TGACCAATTA 
GATTCTAAAT CGAAATCGAA ATCGAAATCA CCCTCACCTT TGAAAAACAA ATTATCTCAC 
ACAAAAGACT TGAAAAATCA AGAATCGGAA AGTATGAGGT TGTCTGATAT GAACAAGAGA 
GATCCAAGAT TAAAAAAACA TCTTCAGGAT AAGACCGATG GCAAAGATGA TGATGTGAAA 
GAGAAGAGAA AAACTGCAGA AAAAAAGGAT AAAGATGAGC ACATGAAGTC ATCCGAACAC 
AGACTGGCTG GAAGTAGAAA TAAAATCATA AATGGCATTG TACAAAAACA GGATACAATA 
ACAGAAGAGT CAGAAAAACA GGGGACAAAA CCAGGGAGAT CGAGTACTAG AAAGCGATCA 
AGATCTCGAT CACCCAAGTC TAGGTCACCA ATTATACATT CCCCAAAGAG AAGAGATAGG 
CGGTCACCCA AACGAAGGCA AAGAAGTATG TCTCCAACAT CGACACCTAA AGCTGGAAAG 
ATTCGCCAAT CTGGAGCTAA GCAGTCACAT ATGGAAGAGT TTACACCACC TTCTAGGGAA 
GACAGAAATG CTAAGAGAAG TACTAAACAG GATATTCGGG ATCCAAGGCG AATGAAAAAG 
ACTGAAGAGG AGCGACCACA AGAAACTACA AATCAGCATT CTACAAAGTC AGGCACTGAA 
CCAAAGGAGA ATGTAGAAAA CTGGCAAAGT TCCAAGTCTG CCAAAAGATG GAAATCTGGT 
TGGGAAGAAA ATAAAAGCTT ACAACAGGTT GATGAACATA GTAAACCTCC TCATCTGAGG 
CATAGGGAGA GCTGGTCAAG CACTAAAGGA ATTTTATCAC CTCGAGCCCC AAAGCAGCAA 
CAGCATCGAT TAAGTGTAGA TGCCAATCTT CAGATTCCTA AAGAGTTAAC TCTTGCAAGC 
AAAAGAGAAT TACTTCAAAA GACGAGTGAA CGTTTAGCAT CTGGTGAAAT TACACAGGAT 
GACTTCCTTG TTGTTGTGCA TCAAATTCGA CAGCTATTTC AGTATCAAGA AGGTAAACAT 
AGATGCAATG TACGGGATAG TCCTACAGAA GAAAATAAAG GTGGATTAAA AAAGAAACCT 
CTCTTATCTG ATGCTGAATT AACCTACTAT GAACATAAAG CAAAACTGAA AAGGACACAG 
GTTCAGCATT CATTTCCAAG ACTTGATCTC TTAGATCCTG ATATTTTTGA CTACCCTTTG 
ACTGATGCCT TGTTGTCTGG AATAGAATGT GAGCCATCCA AAAGTAAACA TGCAAGTAGG 
AATAGTGGAG CACAGTTTGA CAGAAAAGAA CAATTTAGTG AAAGAGCAAG ACGTCTTTCT 
CCTATATCTG GGAGTCGTAC TTATGCTGAG AATCTTTCAC CCCATGAGGG CCGGAGAAGA 
CATGACGAGC AAGTCTCTGC TAAAGGTGTG CGAGAAGAGC AGAGATCTCC ATTCAATGAT 
CGTTTTCCAC TTAAGCGACC TCGATATGAA GATTCAGATA AACCATTTGT AGATAGTCCA 
GCATCAAGAT TCGCCGGCCT GGATACAAAT CAGCGACTTA CAGCTTTAGC TGAAGACAGA 
CCGTTATTTG ATGGACCTAG TAGGCCATCA GTAGCAAGAG ATGGCCCAAC GAAGATGATT 
TTTGAAGGAC CCAATAAATT AAGCCCTCGA ATTGATGGAC CTCCCACACC AGCTTCTCTT 
CGGTTTGATG GGTCACCAGG ACAAATGGGG GGAGGAGGCC CTTTGAGATT TGAGGGGCCA 
CAAGGTCAGC TAGGAGGTGG GTGTCCTTTG AGATTTGAAG GTCCTCCAGG ACCAGTGGGG 
ACACCTCTGC GGTTTGAGGG CCCAATTGGT CAAGCAGGAG GAGGTGGTTT TCGGTTTGAA 
GGTTCCCCTG GTCTGAGGTT TGAGGGATCT CCAGGTGGTT TGAGATTTGA GGGACCAGGA 
GGCCAGCCTG TGGGTGGTCT GAGGTTTGAG GGACATCGTG GTCAACCTGT GGGTGGTCTA 
AGGTTTGAGG GACCTCATGG TCAGCCTGTG GGTGGACTTA GATTTGATAA TCCCCGAGGT 
CAGCCTGTAG GTGGACTTAG ATTTGAGGGG GGTCATGGTC CATCAGGGGC TGCGATTAGG 
TTTGATGGAC CTCATGGTCA GCCAGGAGGT GGAATCAGAT TTGAGGGCCC TTTGCTACAG 
CAAGGGGTTG GAATGAGGTT TGAGGGCCCC CATGGTCAGT CAGTAGCTGG TCTGAGATTT 
GAGGGACAAC ATAATCAACT TGGTGGGAAC CTTAGGTTTG AGGGTCCACA TGGTCAGCCA 
GGGGTTGGTA TCAGGTTTGA AGGCCCTTTA GTCCAACAAG GAGGTGGAAT GAGGTTTGAG 
GGTCCTTCTG TACCAGGAGG TGGCCTGAGA ATTGAAGGGC CTCTGGGTCA AGGTGGTCCA 
AGATTTGAAG GTTGTCATGC TTTAAGGTTT GATGGGCAGC CAGGTCAGCC GTCACTCTTG 
CCAAGATTTG ATGGATTACA TGGTCAGCCA GGTCCTAGAT TTGAAAGGAC TCCTGGTCAG 
CCAGGCCCTC AGAGGTTTGA TGGACCACCT GGACAGCAGG TTCAACCCAG ATTTGACGGT 
GTACCTCAAA GATTTGATGG TCCACAACAT CAGCAAGCAT CAAGGTTTGA TATTCCTCTT 
GGTCTTCAAG GCACAAGATT TGACAATCAT CCTTCACAAA GGCTTGAATC AGTATCTTTC 
AATCAGACTG GTCCATATAA TGATCCACCT GGCAATGCTT TTAATGCCCC ATCCCAAGGA 
CTACAGTTCC AAAGACATGA ACAAATATTT GATTCACCTC AAGGACCAAA TTTTAATGGA 
CCACATGGCC CTGGAAACCA GAGTTTCTCT AATCCACTTA ACAGAGCTTC TGGACACTAT 
TTTGATGAAA AAAATCTTCA GAGTTCTCAA TTTGGAAACT TTGGCAATAT ACCTGCTCCA 
ATGACAGTAG GAAATATTCA GGCATCTCAA CAGGTTCTGA GTGGTGTTGC TCAGCCAGTA 
GCTTTTGGTC AAGGACAACA GTTTTTACCA GTTCATCCAC AAAATCCTGG ATTTGTTCAG 
AATCCTTCAG GAGCCCTCCC TAAGGCATAT CCTGATAATC ATCTCAGTCA GGTGGATGTA 
AATGAATTGT TTTCAAAATT GCTAAAAACA GGAATTCTCA AATTGTCCCA AACTGATTCA 
GCTACAACAC TAAGTGAAGT AACTGCTCAG CCTCCCCCTG AAGAGGAGGA AGATCAAAAT 
GAAGATCAAG ATGTTCCAGA TCTTACTAAT TTTACAGTTG AAGAATTGAA ACAACGTTAT 
GACAGTGTTA TAAATCGACT GTACACTGGT ATTCAGTGTT ACTCTTGTGG AATGAGGTTT 
ACAACATCAC AGACAGATGT TTATGCAGAT CATTTGGACT GGCATTATCG GCAAAATAGA 
ACTGAAAAGG ATGTTAGCCG AAAAGTCACT CATAGACGTT GGTACTACAG TTTAACAGAC 
TGGATAGAAT TTGAGGAGAT AGCTGATCTG GAAGAACGGG CAAAGAGCCA GTTTTTTGAA 
AAGGTGCATG AAGAAGTTGT GCTCAAAACT CAAGAGGCTG CTAAAGAAAA AGAGTTCCAA 
AGTGTACCTG CTGGACCAGC TGGAGCAGTT GAGAGTTGTG AAATCTGTCA AGAACAATTT 
GAACAATACT GGGATGAAGA AGAGGAGGAA TGGCATTTGA AAAATGCTAT TAGAGTAGAT 
GGAAAGATTT ATCATCCATC ATGTTATGAA GATTATCAAA ATACATCTTC ATTTGATTGT 
ACACCATCTC CCAGCAAGAC ACCAGTTGAA AACCCCTTGA ATATTATGTT GAACATTGTC 
AAAAACGAAT TGCAGGAACC CTGTGACAGT CCCAAAGTTA AGGAAGAACG AATTGATACA 
CCACCAGCTT GTACAGAGGA AAGCATAGCA ACACCCTCTG AAATTAAAAC AGAAAATGAC 
ACAGTCGAGT CAGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

WNK1 kinase and the termination factor PCF11 connect nuclear mRNA export with transcription.
Genes & development31(21)2175-2185(2017 Nov)
Volanakis A,Kamieniarz-Gdula K,Schlackow M,Proudfoot NJ

Human Pcf11 enhances degradation of RNA polymerase II-associated nascent RNA and transcriptional termination.
Nucleic acids research36(3)905-14(2008 Feb)
West S,Proudfoot NJ

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PCF11 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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