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PFKFB3 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol PFKFB3
Entrez Gene ID 5209
Full Name 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Synonyms IPFK2,PFK2,iPFK-2
General protein information
Preferred Names

6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3

Names

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3
6-phosphofructo-2-kinase/ fructose-2,6-bisphosphatase
6PF-2-K/Fru-2,6-P2ase 3
6PF-2-K/Fru-2,6-P2ase brain/placenta-type isozyme
PFK/FBPase 3
fructose-6-phosphate,2-kinase/fructose-2, 6-bisphosphatase
inducible 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase
renal carcinoma antigen NY-REN-56

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

10

10p15.1

Summary The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016].
Disorder MIM:

605319


mRNA and Protein(s)

mRNA Protein Name
NM_004566.3 NP_004557.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 1
NM_001323017.1 NP_001309946.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 6
NM_001314063.1 NP_001300992.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 4
NM_001282630.2 NP_001269559.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 3
XM_017016325.1 XP_016871814.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X1
XM_017016327.1 XP_016871816.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X3
XM_005252464.1 XP_005252521.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X6
NM_001323016.1 NP_001309945.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 5
XM_017016326.1 XP_016871815.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X2
XM_017016329.1 XP_016871818.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X5
XM_011519493.1 XP_011517795.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X7
NM_001145443.2 NP_001138915.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2
XM_017016328.1 XP_016871817.1 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X4



Caenorhabditis elegans (roundworm) Y110A7A.6 NP_491529.2
Canis lupus familiaris (dog) PFKFB3 XP_005617225.1
Mus musculus (house mouse) Pfkfb3 NP_001171228.1
Pan troglodytes (chimpanzee) PFKFB3 XP_001147864.2
Rattus norvegicus (Norway rat) Pfkfb3 NP_476476.1
Danio rerio (zebrafish) pfkfb3 NP_998562.1
Homo sapiens (human) PFKFB3 NP_004557.1
Bos taurus (cattle) PFKFB3 NP_001071305.1
Xenopus tropicalis (tropical clawed frog) pfkfb3 NP_001135656.1
Gallus gallus (chicken) PFKFB3 XP_416472.2
Caenorhabditis elegans (roundworm) K02B2.1 NP_500893.2
Macaca mulatta (rhesus monkey) PFKFB3 XP_001107535.1


Related articles in PubMed

PFKFB3 was overexpressed in gastric cancer patients and promoted the proliferation and migration of gastric cancer cells.
Han J, Meng Q, Xi Q, Wang H, Wu G
Cancer biomarkers : section A of Disease markers18(3)249-256(2017)

Akt mediates TIGAR induction in HeLa cells following PFKFB3 inhibition.
Simon-Molas H, Calvo-Vidal MN, Castaño E, Rodríguez-García A, Navarro-Sabaté À, Bartrons R, Manzano A
FEBS letters590(17)2915-26(2016 Sep)

Targeting of MCT1 and PFKFB3 influences cell proliferation and apoptosis in bladder cancer by altering the tumor microenvironment.
Hu KY, Wang de G, Liu PF, Cao YW, Wang YH, Yang XC, Hu CX, Sun LJ, Niu HT
Oncology reports36(2)945-51(2016 Aug)

[EXPRESSION OF PFKFB, HK2, NAMPT, TSPAN13 AND HSPB8 GENES IN PEDIATRIC GLIOMA].
Minchenko DO, Novik YE, Maslak HS, Tiazhka OV, Minchenko OH
Likars'ka sprava(7-8)43-8(2015 Oct-Dec)

6-Phosphofructo-2-kinase (PFKFB3) promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27.
Yalcin A, Clem BF, Imbert-Fernandez Y, Ozcan SC, Peker S, O'Neal J, Klarer AC, Clem AL, Telang S, Chesney J
Cell death & disease5e1337(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Taken together, our study identifies PFKFB3 as a key TGFbeta1 effector protein that mediates TGFbeta1's effect on Snail expression, invasion, and glycolysis.
Title: 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase-3 is required for transforming growth factor β1-enhanced invasion of Panc1 cells in vitro.

PFKFB3 was a novel downstream substrate of mTOR signaling pathway as PFKFB3 level was augmented after knocking down TSC2 in THP1 and OCI-AML3 acute myeloid leukemia cells.
Title: mTOR up-regulation of PFKFB3 is essential for acute myeloid leukemia cell survival.

PFK-2 seems to be a strategic element that links NADPH oxidase activation and glycolysis modulation, and, as such, is proposed as a potential therapeutic target in inflammatory diseases.
Title: Unexpected function of the phagocyte NADPH oxidase in supporting hyperglycolysis in stimulated neutrophils: key role of 6-phosphofructo-2-kinase.

Knockdown of PFKFB3 by siRNAs significantly inhibited the proliferation and migration abilities of gastric cancer cells. Our data suggest that PFKFB3 might be a potential biomarker for gastric cancer and anti-neoplastic targeting gene.
Title: PFKFB3 was overexpressed in gastric cancer patients and promoted the proliferation and migration of gastric cancer cells.

The expression of PFKFB3, PFKFB4, NAMPT, and TSPAN13 is strongly up-regulated in pediatric glioma.
Title: [EXPRESSION OF PFKFB, HK2, NAMPT, TSPAN13 AND HSPB8 GENES IN PEDIATRIC GLIOMA].

The following PFKFB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PFKFB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

Online Only Promotion

Up to 50% OFF! Starting
from $49/clone

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu15166 NM_004566.3
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$219.50-$307.30
$439.00
OHu102778 NM_001323017.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu14576 NM_001282630.2
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu14704 NM_001145443.2
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu43756 NM_001314063.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu43757 XM_005252464.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu59629 XM_011519493.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu102726 NM_001323016.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 9-11 $223.30
$319.00
OHu91463 XM_017016325.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu91464 XM_017016326.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu91465 XM_017016327.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu91466 XM_017016328.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00
OHu91467 XM_017016329.1
Latest version!
Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu15166
Clone ID Related Accession (Same CDS sequence) NM_004566.3
Accession Version NM_004566.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 1
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068734.1, AL157395.17, AK291263.1, AL359960.23 and AI633567.1. On Feb 21, 2009 this sequence version replaced NM_004566.2. Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040482.1, SRR1803616.54660.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
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181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAAACAT GAAAGGCTCC CGGAGCAGCG CTGACTCCTC CAGGAAACAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_004557.1
CDS385..1947
Misc Feature(1)28..30
Misc Feature(2)385..1119
Misc Feature(3)460..1122
Misc Feature(4)order(508..510,523..534)
Misc Feature(5)1120..1944
Misc Feature(6)1129..1680
Misc Feature(7)order(1141..1146,1291..1293,1546..1551)
Misc Feature(8)1141..1143
Misc Feature(9)1162..1164
Misc Feature(10)1546..1548
Misc Feature(11)1765..1767
Misc Feature(12)1771..1773
Misc Feature(13)1783..1785
Misc Feature(14)1795..1797
Exon (1)1..460
Exon (2)461..586
Exon (3)587..683
Exon (4)684..750
Exon (5)751..825
Exon (6)826..882
Exon (7)883..1007
Exon (8)1008..1215
Exon (9)1216..1362
Exon (10)1363..1467
Exon (11)1468..1597
Exon (12)1598..1660
Exon (13)1661..1725
Exon (14)1726..1899
Exon (15)1900..4550
Translation

Target ORF information:

RefSeq Version NM_004566.3
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_004566.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAAACAT GAAAGGCTCC CGGAGCAGCG CTGACTCCTC CAGGAAACAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102778
Clone ID Related Accession (Same CDS sequence) NM_001323017.1
Accession Version NM_001323017.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1014bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 6
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157395.17 and AL359960.23. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2149004, SAMEA2150385 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACGACT TCATGAAGAG GATCAGTTGC TATGAAGCCA GCTACCAGCC CCTCGACCCC 
GACAAATGCG ACAGGGACTT GTCGCTGATC AAGGTGATTG ACGTGGGCCG GAGGTTCCTG
GTGAACCGGG TGCAGGACCA CATCCAGAGC CGCATCGTGT ACTACCTGAT GAACATCCAC
GTGCAGCCGC GTACCATCTA CCTGTGCCGG CACGGCGAGA ACGAGCACAA CCTCCAGGGC
CGCATCGGGG GCGACTCAGG CCTGTCCAGC CGGGGCAAGA AGTTTGCCAG TGCTCTGAGC
AAGTTCGTGG AGGAGCAGAA CCTGAAGGAC CTGCGCGTGT GGACCAGCCA GCTGAAGAGC
ACCATCCAGA CGGCCGAGGC GCTGCGGCTG CCCTACGAGC AGTGGAAGGC GCTCAATGAG
ATCGACGCGG GCGTCTGTGA GGAGCTGACC TACGAGGAGA TCAGGGACAC CTACCCTGAG
GAGTATGCGC TGCGGGAGCA GGACAAGTAC TATTACCGCT ACCCCACCGG GGAGTCCTAC
CAGGACCTGG TCCAGCGCTT GGAGCCAGTG ATCATGGAGC TGGAGCGGCA GGAGAATGTG
CTGGTCATCT GCCACCAGGC CGTCCTGCGC TGCCTGCTTG CCTACTTCCT GGATAAGAGT
GCAGAGGAGA TGCCCTACCT GAAATGCCCT CTTCACACCG TCCTGAAACT GACGCCTGTC
GCTTATGGCT GCCGTGTGGA ATCCATCTAC CTGAACGTGG AGTCCGTCTG CACACACCGG
GAGAGGTCAG AGGATGCAAA GAAGGGACCT AACCCGCTCA TGAGACGCAA TAGTGTCACC
CCGCTAGCCA GCCCCGAACC CACCAAAAAG CCTCGCATCA ACAGCTTTGA GGAGCATGTG
GCCTCCACCT CGGCCGCCCT GCCCAGCTGC CTGCCCCCGG AGGTGCCCAC GCAGCTGCCT
GGACAAAACA TGAAAGGCTC CCGGAGCAGC GCTGACTCCT CCAGGAAACA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001309946.1
CDS984..1997
Misc Feature(1)900..902
Exon (1)1..460
Exon (2)461..586
Exon (3)587..683
Exon (4)684..750
Exon (5)751..825
Exon (6)826..882
Exon (7)883..1057
Exon (8)1058..1265
Exon (9)1266..1412
Exon (10)1413..1517
Exon (11)1518..1647
Exon (12)1648..1710
Exon (13)1711..1775
Exon (14)1776..1949
Exon (15)1950..4600
Translation

Target ORF information:

RefSeq Version NM_001323017.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001323017.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
ATGGACGACT TCATGAAGAG GATCAGTTGC TATGAAGCCA GCTACCAGCC CCTCGACCCC 
GACAAATGCG ACAGGGACTT GTCGCTGATC AAGGTGATTG ACGTGGGCCG GAGGTTCCTG
GTGAACCGGG TGCAGGACCA CATCCAGAGC CGCATCGTGT ACTACCTGAT GAACATCCAC
GTGCAGCCGC GTACCATCTA CCTGTGCCGG CACGGCGAGA ACGAGCACAA CCTCCAGGGC
CGCATCGGGG GCGACTCAGG CCTGTCCAGC CGGGGCAAGA AGTTTGCCAG TGCTCTGAGC
AAGTTCGTGG AGGAGCAGAA CCTGAAGGAC CTGCGCGTGT GGACCAGCCA GCTGAAGAGC
ACCATCCAGA CGGCCGAGGC GCTGCGGCTG CCCTACGAGC AGTGGAAGGC GCTCAATGAG
ATCGACGCGG GCGTCTGTGA GGAGCTGACC TACGAGGAGA TCAGGGACAC CTACCCTGAG
GAGTATGCGC TGCGGGAGCA GGACAAGTAC TATTACCGCT ACCCCACCGG GGAGTCCTAC
CAGGACCTGG TCCAGCGCTT GGAGCCAGTG ATCATGGAGC TGGAGCGGCA GGAGAATGTG
CTGGTCATCT GCCACCAGGC CGTCCTGCGC TGCCTGCTTG CCTACTTCCT GGATAAGAGT
GCAGAGGAGA TGCCCTACCT GAAATGCCCT CTTCACACCG TCCTGAAACT GACGCCTGTC
GCTTATGGCT GCCGTGTGGA ATCCATCTAC CTGAACGTGG AGTCCGTCTG CACACACCGG
GAGAGGTCAG AGGATGCAAA GAAGGGACCT AACCCGCTCA TGAGACGCAA TAGTGTCACC
CCGCTAGCCA GCCCCGAACC CACCAAAAAG CCTCGCATCA ACAGCTTTGA GGAGCATGTG
GCCTCCACCT CGGCCGCCCT GCCCAGCTGC CTGCCCCCGG AGGTGCCCAC GCAGCTGCCT
GGACAAAACA TGAAAGGCTC CCGGAGCAGC GCTGACTCCT CCAGGAAACA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14576
Clone ID Related Accession (Same CDS sequence) NM_001282630.2
Accession Version NM_001282630.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1605bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 3
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC316967.1, AK304450.1, AK303039.1, AL359960.23 and AI633567.1. On Oct 1, 2015 this sequence version replaced NM_001282630.1. Transcript Variant: This variant (3) uses an alternate 5' terminal exon compared to variant 1. The resulting isoform (3) has a longer and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304450.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGGAGG GTGGCCAGAA GGAAGGTGAC AGCCAGCAAG CAGGGGCTCT GCCACTCCTC 
TGTCAGCTGG ACACGTTTAG TCCCAAGGCC ACTGTCTTCG GTGTCTCCAT TAATCCAGCC
TGTGGGCCAA AGCTGACCAA CTCCCCCACC GTCATCGTCA TGGTGGGCCT CCCCGCCCGG
GGCAAGACCT ACATCTCCAA GAAGCTGACT CGCTACCTCA ACTGGATTGG CGTCCCCACA
AAAGTGTTCA ACGTCGGGGA GTATCGCCGG GAGGCTGTGA AGCAGTACAG CTCCTACAAC
TTCTTCCGCC CCGACAATGA GGAAGCCATG AAAGTCCGGA AGCAATGTGC CTTAGCTGCC
TTGAGAGATG TCAAAAGCTA CCTGGCGAAA GAAGGGGGAC AAATTGCGGT TTTCGATGCC
ACCAATACTA CTAGAGAGAG GAGACACATG ATCCTTCATT TTGCCAAAGA AAATGACTTT
AAGGCGTTTT TCATCGAGTC GGTGTGCGAC GACCCTACAG TTGTGGCCTC CAATATCATG
GAAGTTAAAA TCTCCAGCCC GGATTACAAA GACTGCAACT CGGCAGAAGC CATGGACGAC
TTCATGAAGA GGATCAGTTG CTATGAAGCC AGCTACCAGC CCCTCGACCC CGACAAATGC
GACAGGGACT TGTCGCTGAT CAAGGTGATT GACGTGGGCC GGAGGTTCCT GGTGAACCGG
GTGCAGGACC ACATCCAGAG CCGCATCGTG TACTACCTGA TGAACATCCA CGTGCAGCCG
CGTACCATCT ACCTGTGCCG GCACGGCGAG AACGAGCACA ACCTCCAGGG CCGCATCGGG
GGCGACTCAG GCCTGTCCAG CCGGGGCAAG AAGTTTGCCA GTGCTCTGAG CAAGTTCGTG
GAGGAGCAGA ACCTGAAGGA CCTGCGCGTG TGGACCAGCC AGCTGAAGAG CACCATCCAG
ACGGCCGAGG CGCTGCGGCT GCCCTACGAG CAGTGGAAGG CGCTCAATGA GATCGACGCG
GGCGTCTGTG AGGAGCTGAC CTACGAGGAG ATCAGGGACA CCTACCCTGA GGAGTATGCG
CTGCGGGAGC AGGACAAGTA CTATTACCGC TACCCCACCG GGGAGTCCTA CCAGGACCTG
GTCCAGCGCT TGGAGCCAGT GATCATGGAG CTGGAGCGGC AGGAGAATGT GCTGGTCATC
TGCCACCAGG CCGTCCTGCG CTGCCTGCTT GCCTACTTCC TGGATAAGAG TGCAGAGGAG
ATGCCCTACC TGAAATGCCC TCTTCACACC GTCCTGAAAC TGACGCCTGT CGCTTATGGC
TGCCGTGTGG AATCCATCTA CCTGAACGTG GAGTCCGTCT GCACACACCG GGAGAGGTCA
GAGGATGCAA AGAAGGGACC TAACCCGCTC ATGAGACGCA ATAGTGTCAC CCCGCTAGCC
AGCCCCGAAC CCACCAAAAA GCCTCGCATC AACAGCTTTG AGGAGCATGT GGCCTCCACC
TCGGCCGCCC TGCCCAGCTG CCTGCCCCCG GAGGTGCCCA CGCAGCTGCC TGGACAAAAC
ATGAAAGGCT CCCGGAGCAG CGCTGACTCC TCCAGGAAAC ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001269559.1
CDS132..1736
Misc Feature(1)87..89
Misc Feature(2)252..911
Misc Feature(3)order(297..299,312..323)
Misc Feature(4)918..1469
Misc Feature(5)order(930..935,1080..1082,1335..1340)
Exon (1)1..249
Exon (2)250..375
Exon (3)376..472
Exon (4)473..539
Exon (5)540..614
Exon (6)615..671
Exon (7)672..796
Exon (8)797..1004
Exon (9)1005..1151
Exon (10)1152..1256
Exon (11)1257..1386
Exon (12)1387..1449
Exon (13)1450..1514
Exon (14)1515..1688
Exon (15)1689..4339
Translation

Target ORF information:

RefSeq Version NM_001282630.2
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001282630.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGGGGAGG GTGGCCAGAA GGAAGGTGAC AGCCAGCAAG CAGGGGCTCT GCCACTCCTC 
TGTCAGCTGG ACACGTTTAG TCCCAAGGCC ACTGTCTTCG GTGTCTCCAT TAATCCAGCC
TGTGGGCCAA AGCTGACCAA CTCCCCCACC GTCATCGTCA TGGTGGGCCT CCCCGCCCGG
GGCAAGACCT ACATCTCCAA GAAGCTGACT CGCTACCTCA ACTGGATTGG CGTCCCCACA
AAAGTGTTCA ACGTCGGGGA GTATCGCCGG GAGGCTGTGA AGCAGTACAG CTCCTACAAC
TTCTTCCGCC CCGACAATGA GGAAGCCATG AAAGTCCGGA AGCAATGTGC CTTAGCTGCC
TTGAGAGATG TCAAAAGCTA CCTGGCGAAA GAAGGGGGAC AAATTGCGGT TTTCGATGCC
ACCAATACTA CTAGAGAGAG GAGACACATG ATCCTTCATT TTGCCAAAGA AAATGACTTT
AAGGCGTTTT TCATCGAGTC GGTGTGCGAC GACCCTACAG TTGTGGCCTC CAATATCATG
GAAGTTAAAA TCTCCAGCCC GGATTACAAA GACTGCAACT CGGCAGAAGC CATGGACGAC
TTCATGAAGA GGATCAGTTG CTATGAAGCC AGCTACCAGC CCCTCGACCC CGACAAATGC
GACAGGGACT TGTCGCTGAT CAAGGTGATT GACGTGGGCC GGAGGTTCCT GGTGAACCGG
GTGCAGGACC ACATCCAGAG CCGCATCGTG TACTACCTGA TGAACATCCA CGTGCAGCCG
CGTACCATCT ACCTGTGCCG GCACGGCGAG AACGAGCACA ACCTCCAGGG CCGCATCGGG
GGCGACTCAG GCCTGTCCAG CCGGGGCAAG AAGTTTGCCA GTGCTCTGAG CAAGTTCGTG
GAGGAGCAGA ACCTGAAGGA CCTGCGCGTG TGGACCAGCC AGCTGAAGAG CACCATCCAG
ACGGCCGAGG CGCTGCGGCT GCCCTACGAG CAGTGGAAGG CGCTCAATGA GATCGACGCG
GGCGTCTGTG AGGAGCTGAC CTACGAGGAG ATCAGGGACA CCTACCCTGA GGAGTATGCG
CTGCGGGAGC AGGACAAGTA CTATTACCGC TACCCCACCG GGGAGTCCTA CCAGGACCTG
GTCCAGCGCT TGGAGCCAGT GATCATGGAG CTGGAGCGGC AGGAGAATGT GCTGGTCATC
TGCCACCAGG CCGTCCTGCG CTGCCTGCTT GCCTACTTCC TGGATAAGAG TGCAGAGGAG
ATGCCCTACC TGAAATGCCC TCTTCACACC GTCCTGAAAC TGACGCCTGT CGCTTATGGC
TGCCGTGTGG AATCCATCTA CCTGAACGTG GAGTCCGTCT GCACACACCG GGAGAGGTCA
GAGGATGCAA AGAAGGGACC TAACCCGCTC ATGAGACGCA ATAGTGTCAC CCCGCTAGCC
AGCCCCGAAC CCACCAAAAA GCCTCGCATC AACAGCTTTG AGGAGCATGT GGCCTCCACC
TCGGCCGCCC TGCCCAGCTG CCTGCCCCCG GAGGTGCCCA CGCAGCTGCC TGGACAAAAC
ATGAAAGGCT CCCGGAGCAG CGCTGACTCC TCCAGGAAAC ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu14704
Clone ID Related Accession (Same CDS sequence) NM_001145443.2
Accession Version NM_001145443.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1503bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 2
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157395.17, DC319465.1, AK303039.1, AL359960.23 and AI633567.1. On Oct 1, 2015 this sequence version replaced NM_001145443.1. Transcript Variant: This variant (2) uses an alternate 5' terminal exon compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCCTTCA GGAAAGCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG 
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAAACAT GAAAGGCTCC CGGAGCAGCG CTGACTCCTC CAGGAAACAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001138915.1
CDS121..1623
Misc Feature(1)130..798
Misc Feature(2)order(184..186,199..210)
Misc Feature(3)805..1356
Misc Feature(4)order(817..822,967..969,1222..1227)
Exon (1)1..136
Exon (2)137..262
Exon (3)263..359
Exon (4)360..426
Exon (5)427..501
Exon (6)502..558
Exon (7)559..683
Exon (8)684..891
Exon (9)892..1038
Exon (10)1039..1143
Exon (11)1144..1273
Exon (12)1274..1336
Exon (13)1337..1401
Exon (14)1402..1575
Exon (15)1576..4226
Translation

Target ORF information:

RefSeq Version NM_001145443.2
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001145443.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCCTTCA GGAAAGCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG 
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAAACAT GAAAGGCTCC CGGAGCAGCG CTGACTCCTC CAGGAAACAC
TGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43756
Clone ID Related Accession (Same CDS sequence) NM_001314063.1
Accession Version NM_001314063.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1545bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 4
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB068734.1, AL157395.17, AF056320.1, AL359960.23 and AI633567.1. On Oct 1, 2015 this sequence version replaced XM_005252463.1. Transcript Variant: This variant (4) differs in the 3' UTR and contains an alternate exon in the 3' coding region that results in a frameshift, compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.209395.1, SRR1803611.52479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAACCTTT GCTAGGGCAA GCCTGTCTAA CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001300992.1
CDS385..1929
Misc Feature(1)28..30
Misc Feature(2)385..1119
Misc Feature(3)460..1122
Misc Feature(4)order(508..510,523..534)
Misc Feature(5)1129..1680
Misc Feature(6)order(1141..1146,1291..1293,1546..1551)
Misc Feature(7)1141..1143
Misc Feature(8)1162..1164
Misc Feature(9)1546..1548
Misc Feature(10)1765..1767
Misc Feature(11)1771..1773
Misc Feature(12)1783..1785
Misc Feature(13)1795..1797
Exon (1)1..460
Exon (2)461..586
Exon (3)587..683
Exon (4)684..750
Exon (5)751..825
Exon (6)826..882
Exon (7)883..1007
Exon (8)1008..1215
Exon (9)1216..1362
Exon (10)1363..1467
Exon (11)1468..1597
Exon (12)1598..1660
Exon (13)1661..1725
Exon (14)1726..1899
Exon (15)1900..1922
Exon (16)1923..4573
Translation

Target ORF information:

RefSeq Version NM_001314063.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001314063.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAACCTTT GCTAGGGCAA GCCTGTCTAA CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu43757
Clone ID Related Accession (Same CDS sequence) XM_005252464.1
Accession Version XM_005252464.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1389bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X6
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GAACATGAAA GGCTCCCGGA GCAGCGCTGA CTCCTCCAGG
AAACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005252521.1
CDS385..1773
Misc Feature(1)460..1122
Misc Feature(2)order(508..510,523..534)
Misc Feature(3)1129..1680
Misc Feature(4)order(1141..1146,1291..1293,1546..1551)
Translation

Target ORF information:

RefSeq Version XM_005252464.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_005252464.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GAACATGAAA GGCTCCCGGA GCAGCGCTGA CTCCTCCAGG
AAACACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59629
Clone ID Related Accession (Same CDS sequence) XM_011519493.1
Accession Version XM_011519493.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1284bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X7
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGAACA TGAAAGGCTC CCGGAGCAGC
GCTGACTCCT CCAGGAAACA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011517795.1
CDS385..1668
Misc Feature(1)460..1122
Misc Feature(2)order(508..510,523..534)
Misc Feature(3)1126..1581
Misc Feature(4)order(1141..1146,1291..1293,1441..1446)
Translation

Target ORF information:

RefSeq Version XM_011519493.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011519493.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGAACA TGAAAGGCTC CCGGAGCAGC
GCTGACTCCT CCAGGAAACA CTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu102726
Clone ID Related Accession (Same CDS sequence) NM_001323016.1
Accession Version NM_001323016.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1485bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-05-07
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157395.17 and AL359960.23. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCCTTCA GGAAAGCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG 
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAACCTTT GCTAGGGCAA GCCTGTCTAA CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001309945.1
CDS121..1605
Exon (1)1..136
Exon (2)137..262
Exon (3)263..359
Exon (4)360..426
Exon (5)427..501
Exon (6)502..558
Exon (7)559..683
Exon (8)684..891
Exon (9)892..1038
Exon (10)1039..1143
Exon (11)1144..1273
Exon (12)1274..1336
Exon (13)1337..1401
Exon (14)1402..1575
Exon (15)1576..1598
Exon (16)1599..4249
Translation

Target ORF information:

RefSeq Version NM_001323016.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001323016.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGCCCTTCA GGAAAGCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG 
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAACCTTT GCTAGGGCAA GCCTGTCTAA CATGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu91463
Clone ID Related Accession (Same CDS sequence) XM_017016325.1
Accession Version XM_017016325.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1707bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAGAGTT AAAGAGTGGA ATTCCTGGAA GGGACCCTGA GCCACCAGTC
CAGCCCCCTG AAGGGCAGAG CATTCTCTGG ATACCCAGAG AGGTGACCGT GGCATCCAGG
AGTGGACCCG GGGCTGCACC AAGCATCCCG GAGCCCAGAC TGGTCTCGCT TCCTCACCTT
TCTGGCTCTG CGGCAAGCGG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871814.1
CDS385..2091
Translation

Target ORF information:

RefSeq Version XM_017016325.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017016325.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAGAGTT AAAGAGTGGA ATTCCTGGAA GGGACCCTGA GCCACCAGTC
CAGCCCCCTG AAGGGCAGAG CATTCTCTGG ATACCCAGAG AGGTGACCGT GGCATCCAGG
AGTGGACCCG GGGCTGCACC AAGCATCCCG GAGCCCAGAC TGGTCTCGCT TCCTCACCTT
TCTGGCTCTG CGGCAAGCGG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu91464
Clone ID Related Accession (Same CDS sequence) XM_017016326.1
Accession Version XM_017016326.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1650bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGAAGTTAAA ATCTCCAGCC CGGATTACAA AGACTGCAAC
TCGGCAGAAG CCATGGACGA CTTCATGAAG AGGATCAGTT GCTATGAAGC CAGCTACCAG
CCCCTCGACC CCGACAAATG CGACAGGGAC TTGTCGCTGA TCAAGGTGAT TGACGTGGGC
CGGAGGTTCC TGGTGAACCG GGTGCAGGAC CACATCCAGA GCCGCATCGT GTACTACCTG
ATGAACATCC ACGTGCAGCC GCGTACCATC TACCTGTGCC GGCACGGCGA GAACGAGCAC
AACCTCCAGG GCCGCATCGG GGGCGACTCA GGCCTGTCCA GCCGGGGCAA GAAGTTTGCC
AGTGCTCTGA GCAAGTTCGT GGAGGAGCAG AACCTGAAGG ACCTGCGCGT GTGGACCAGC
CAGCTGAAGA GCACCATCCA GACGGCCGAG GCGCTGCGGC TGCCCTACGA GCAGTGGAAG
GCGCTCAATG AGATCGACGC GGGCGTCTGT GAGGAGCTGA CCTACGAGGA GATCAGGGAC
ACCTACCCTG AGGAGTATGC GCTGCGGGAG CAGGACAAGT ACTATTACCG CTACCCCACC
GGGGAGTCCT ACCAGGACCT GGTCCAGCGC TTGGAGCCAG TGATCATGGA GCTGGAGCGG
CAGGAGAATG TGCTGGTCAT CTGCCACCAG GCCGTCCTGC GCTGCCTGCT TGCCTACTTC
CTGGATAAGA GTGCAGAGGA GATGCCCTAC CTGAAATGCC CTCTTCACAC CGTCCTGAAA
CTGACGCCTG TCGCTTATGG CTGCCGTGTG GAATCCATCT ACCTGAACGT GGAGTCCGTC
TGCACACACC GGGAGAGGTC AGAGGATGCA AAGAAGGGAC CTAACCCGCT CATGAGACGC
AATAGTGTCA CCCCGCTAGC CAGCCCCGAA CCCACCAAAA AGCCTCGCAT CAACAGCTTT
GAGGAGCATG TGGCCTCCAC CTCGGCCGCC CTGCCCAGCT GCCTGCCCCC GGAGGTGCCC
ACGCAGCTGC CTGGACAAGA GTTAAAGAGT GGAATTCCTG GAAGGGACCC TGAGCCACCA
GTCCAGCCCC CTGAAGGGCA GAGCATTCTC TGGATACCCA GAGAGGTGAC CGTGGCATCC
AGGAGTGGAC CCGGGGCTGC ACCAAGCATC CCGGAGCCCA GACTGGTCTC GCTTCCTCAC
CTTTCTGGCT CTGCGGCAAG CGGCTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871815.1
CDS385..2034
Translation

Target ORF information:

RefSeq Version XM_017016326.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017016326.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGAAGTTAAA ATCTCCAGCC CGGATTACAA AGACTGCAAC
TCGGCAGAAG CCATGGACGA CTTCATGAAG AGGATCAGTT GCTATGAAGC CAGCTACCAG
CCCCTCGACC CCGACAAATG CGACAGGGAC TTGTCGCTGA TCAAGGTGAT TGACGTGGGC
CGGAGGTTCC TGGTGAACCG GGTGCAGGAC CACATCCAGA GCCGCATCGT GTACTACCTG
ATGAACATCC ACGTGCAGCC GCGTACCATC TACCTGTGCC GGCACGGCGA GAACGAGCAC
AACCTCCAGG GCCGCATCGG GGGCGACTCA GGCCTGTCCA GCCGGGGCAA GAAGTTTGCC
AGTGCTCTGA GCAAGTTCGT GGAGGAGCAG AACCTGAAGG ACCTGCGCGT GTGGACCAGC
CAGCTGAAGA GCACCATCCA GACGGCCGAG GCGCTGCGGC TGCCCTACGA GCAGTGGAAG
GCGCTCAATG AGATCGACGC GGGCGTCTGT GAGGAGCTGA CCTACGAGGA GATCAGGGAC
ACCTACCCTG AGGAGTATGC GCTGCGGGAG CAGGACAAGT ACTATTACCG CTACCCCACC
GGGGAGTCCT ACCAGGACCT GGTCCAGCGC TTGGAGCCAG TGATCATGGA GCTGGAGCGG
CAGGAGAATG TGCTGGTCAT CTGCCACCAG GCCGTCCTGC GCTGCCTGCT TGCCTACTTC
CTGGATAAGA GTGCAGAGGA GATGCCCTAC CTGAAATGCC CTCTTCACAC CGTCCTGAAA
CTGACGCCTG TCGCTTATGG CTGCCGTGTG GAATCCATCT ACCTGAACGT GGAGTCCGTC
TGCACACACC GGGAGAGGTC AGAGGATGCA AAGAAGGGAC CTAACCCGCT CATGAGACGC
AATAGTGTCA CCCCGCTAGC CAGCCCCGAA CCCACCAAAA AGCCTCGCAT CAACAGCTTT
GAGGAGCATG TGGCCTCCAC CTCGGCCGCC CTGCCCAGCT GCCTGCCCCC GGAGGTGCCC
ACGCAGCTGC CTGGACAAGA GTTAAAGAGT GGAATTCCTG GAAGGGACCC TGAGCCACCA
GTCCAGCCCC CTGAAGGGCA GAGCATTCTC TGGATACCCA GAGAGGTGAC CGTGGCATCC
AGGAGTGGAC CCGGGGCTGC ACCAAGCATC CCGGAGCCCA GACTGGTCTC GCTTCCTCAC
CTTTCTGGCT CTGCGGCAAG CGGCTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu91465
Clone ID Related Accession (Same CDS sequence) XM_017016327.1
Accession Version XM_017016327.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1647bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCCTTCA GGAAAGCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG 
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAGAGTT AAAGAGTGGA ATTCCTGGAA GGGACCCTGA GCCACCAGTC
CAGCCCCCTG AAGGGCAGAG CATTCTCTGG ATACCCAGAG AGGTGACCGT GGCATCCAGG
AGTGGACCCG GGGCTGCACC AAGCATCCCG GAGCCCAGAC TGGTCTCGCT TCCTCACCTT
TCTGGCTCTG CGGCAAGCGG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871816.1
CDS197..1843
Translation

Target ORF information:

RefSeq Version XM_017016327.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017016327.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
ATGCCCTTCA GGAAAGCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG 
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGGG CGTCTGTGAG GAGCTGACCT ACGAGGAGAT CAGGGACACC
TACCCTGAGG AGTATGCGCT GCGGGAGCAG GACAAGTACT ATTACCGCTA CCCCACCGGG
GAGTCCTACC AGGACCTGGT CCAGCGCTTG GAGCCAGTGA TCATGGAGCT GGAGCGGCAG
GAGAATGTGC TGGTCATCTG CCACCAGGCC GTCCTGCGCT GCCTGCTTGC CTACTTCCTG
GATAAGAGTG CAGAGGAGAT GCCCTACCTG AAATGCCCTC TTCACACCGT CCTGAAACTG
ACGCCTGTCG CTTATGGCTG CCGTGTGGAA TCCATCTACC TGAACGTGGA GTCCGTCTGC
ACACACCGGG AGAGGTCAGA GGATGCAAAG AAGGGACCTA ACCCGCTCAT GAGACGCAAT
AGTGTCACCC CGCTAGCCAG CCCCGAACCC ACCAAAAAGC CTCGCATCAA CAGCTTTGAG
GAGCATGTGG CCTCCACCTC GGCCGCCCTG CCCAGCTGCC TGCCCCCGGA GGTGCCCACG
CAGCTGCCTG GACAAGAGTT AAAGAGTGGA ATTCCTGGAA GGGACCCTGA GCCACCAGTC
CAGCCCCCTG AAGGGCAGAG CATTCTCTGG ATACCCAGAG AGGTGACCGT GGCATCCAGG
AGTGGACCCG GGGCTGCACC AAGCATCCCG GAGCCCAGAC TGGTCTCGCT TCCTCACCTT
TCTGGCTCTG CGGCAAGCGG CTCCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu91466
Clone ID Related Accession (Same CDS sequence) XM_017016328.1
Accession Version XM_017016328.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1602bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGGATG CAAAGAAGGG ACCTAACCCG
CTCATGAGAC GCAATAGTGT CACCCCGCTA GCCAGCCCCG AACCCACCAA AAAGCCTCGC
ATCAACAGCT TTGAGGAGCA TGTGGCCTCC ACCTCGGCCG CCCTGCCCAG CTGCCTGCCC
CCGGAGGTGC CCACGCAGCT GCCTGGACAA GAGTTAAAGA GTGGAATTCC TGGAAGGGAC
CCTGAGCCAC CAGTCCAGCC CCCTGAAGGG CAGAGCATTC TCTGGATACC CAGAGAGGTG
ACCGTGGCAT CCAGGAGTGG ACCCGGGGCT GCACCAAGCA TCCCGGAGCC CAGACTGGTC
TCGCTTCCTC ACCTTTCTGG CTCTGCGGCA AGCGGCTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871817.1
CDS385..1986
Translation

Target ORF information:

RefSeq Version XM_017016328.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017016328.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGCGTTTTTC ATCGAGTCGG TGTGCGACGA CCCTACAGTT
GTGGCCTCCA ATATCATGGA AGTTAAAATC TCCAGCCCGG ATTACAAAGA CTGCAACTCG
GCAGAAGCCA TGGACGACTT CATGAAGAGG ATCAGTTGCT ATGAAGCCAG CTACCAGCCC
CTCGACCCCG ACAAATGCGA CAGGGACTTG TCGCTGATCA AGGTGATTGA CGTGGGCCGG
AGGTTCCTGG TGAACCGGGT GCAGGACCAC ATCCAGAGCC GCATCGTGTA CTACCTGATG
AACATCCACG TGCAGCCGCG TACCATCTAC CTGTGCCGGC ACGGCGAGAA CGAGCACAAC
CTCCAGGGCC GCATCGGGGG CGACTCAGGC CTGTCCAGCC GGGGCAAGAA GTTTGCCAGT
GCTCTGAGCA AGTTCGTGGA GGAGCAGAAC CTGAAGGACC TGCGCGTGTG GACCAGCCAG
CTGAAGAGCA CCATCCAGAC GGCCGAGGCG CTGCGGCTGC CCTACGAGCA GTGGAAGGCG
CTCAATGAGA TCGACGCGTC CTACCAGGAC CTGGTCCAGC GCTTGGAGCC AGTGATCATG
GAGCTGGAGC GGCAGGAGAA TGTGCTGGTC ATCTGCCACC AGGCCGTCCT GCGCTGCCTG
CTTGCCTACT TCCTGGATAA GAGTGCAGAG GAGATGCCCT ACCTGAAATG CCCTCTTCAC
ACCGTCCTGA AACTGACGCC TGTCGCTTAT GGCTGCCGTG TGGAATCCAT CTACCTGAAC
GTGGAGTCCG TCTGCACACA CCGGGAGAGG TCAGAGGATG CAAAGAAGGG ACCTAACCCG
CTCATGAGAC GCAATAGTGT CACCCCGCTA GCCAGCCCCG AACCCACCAA AAAGCCTCGC
ATCAACAGCT TTGAGGAGCA TGTGGCCTCC ACCTCGGCCG CCCTGCCCAG CTGCCTGCCC
CCGGAGGTGC CCACGCAGCT GCCTGGACAA GAGTTAAAGA GTGGAATTCC TGGAAGGGAC
CCTGAGCCAC CAGTCCAGCC CCCTGAAGGG CAGAGCATTC TCTGGATACC CAGAGAGGTG
ACCGTGGCAT CCAGGAGTGG ACCCGGGGCT GCACCAAGCA TCCCGGAGCC CAGACTGGTC
TCGCTTCCTC ACCTTTCTGG CTCTGCGGCA AGCGGCTCCT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu91467
Clone ID Related Accession (Same CDS sequence) XM_017016329.1
Accession Version XM_017016329.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1506bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGAAGTTAAA ATCTCCAGCC CGGATTACAA AGACTGCAAC
TCGGCAGAAG CCATGGACGA CTTCATGAAG AGGATCAGTT GCTATGAAGC CAGCTACCAG
CCCCTCGACC CCGACAAATG CGACAGGGAC TTGTCGCTGA TCAAGGTGAT TGACGTGGGC
CGGAGGTTCC TGGTGAACCG GGTGCAGGAC CACATCCAGA GCCGCATCGT GTACTACCTG
ATGAACATCC ACGTGCAGCC GCGTACCATC TACCTGTGCC GGCACGGCGA GAACGAGCAC
AACCTCCAGG GCCGCATCGG GGGCGACTCA GGCCTGTCCA GCCGGGGCAA GAAGTTTGCC
AGTGCTCTGA GCAAGTTCGT GGAGGAGCAG AACCTGAAGG ACCTGCGCGT GTGGACCAGC
CAGCTGAAGA GCACCATCCA GACGGCCGAG GCGCTGCGGC TGCCCTACGA GCAGTGGAAG
GCGCTCAATG AGATCGACGC GGGCGTCTGT GAGGAGCTGA CCTACGAGGA GATCAGGGAC
ACCTACCCTG AGGAGTATGC GCTGCGGGAG CAGGACAAGT ACTATTACCG CTACCCCACC
GGGGAGTCCT ACCAGGACCT GGTCCAGCGC TTGGAGCCAG TGATCATGGA GCTGGAGCGG
CAGGAGAATG TGCTGGTCAT CTGCCACCAG GCCGTCCTGC GCTGCCTGCT TGCCTACTTC
CTGGATAAGA GTGCAGAGGA GATGCCCTAC CTGAAATGCC CTCTTCACAC CGTCCTGAAA
CTGACGCCTG TCGCTTATGG CTGCCGTGTG GAATCCATCT ACCTGAACGT GGAGTCCGTC
TGCACACACC GGGAGAGGTC AGAGGATGCA AAGAAGGGAC CTAACCCGCT CATGAGACGC
AATAGTGTCA CCCCGCTAGC CAGCCCCGAA CCCACCAAAA AGCCTCGCAT CAACAGCTTT
GAGGAGCATG TGGCCTCCAC CTCGGCCGCC CTGCCCAGCT GCCTGCCCCC GGAGGTGCCC
ACGCAGCTGC CTGGACAAAA CATGAAAGGC TCCCGGAGCA GCGCTGACTC CTCCAGGAAA
CACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016871818.1
CDS385..1890
Translation

Target ORF information:

RefSeq Version XM_017016329.1
Organism Homo sapiens(Human)
Definition Homo sapiens 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017016329.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGCCGTTGG AACTGACGCA GAGCCGAGTG CAGAAGATCT GGGTGCCCGT GGACCACAGG 
CCCTCGTTGC CCAGATCCTG TGGGCCAAAG CTGACCAACT CCCCCACCGT CATCGTCATG
GTGGGCCTCC CCGCCCGGGG CAAGACCTAC ATCTCCAAGA AGCTGACTCG CTACCTCAAC
TGGATTGGCG TCCCCACAAA AGTGTTCAAC GTCGGGGAGT ATCGCCGGGA GGCTGTGAAG
CAGTACAGCT CCTACAACTT CTTCCGCCCC GACAATGAGG AAGCCATGAA AGTCCGGAAG
CAATGTGCCT TAGCTGCCTT GAGAGATGTC AAAAGCTACC TGGCGAAAGA AGGGGGACAA
ATTGCGGTTT TCGATGCCAC CAATACTACT AGAGAGAGGA GACACATGAT CCTTCATTTT
GCCAAAGAAA ATGACTTTAA GGAAGTTAAA ATCTCCAGCC CGGATTACAA AGACTGCAAC
TCGGCAGAAG CCATGGACGA CTTCATGAAG AGGATCAGTT GCTATGAAGC CAGCTACCAG
CCCCTCGACC CCGACAAATG CGACAGGGAC TTGTCGCTGA TCAAGGTGAT TGACGTGGGC
CGGAGGTTCC TGGTGAACCG GGTGCAGGAC CACATCCAGA GCCGCATCGT GTACTACCTG
ATGAACATCC ACGTGCAGCC GCGTACCATC TACCTGTGCC GGCACGGCGA GAACGAGCAC
AACCTCCAGG GCCGCATCGG GGGCGACTCA GGCCTGTCCA GCCGGGGCAA GAAGTTTGCC
AGTGCTCTGA GCAAGTTCGT GGAGGAGCAG AACCTGAAGG ACCTGCGCGT GTGGACCAGC
CAGCTGAAGA GCACCATCCA GACGGCCGAG GCGCTGCGGC TGCCCTACGA GCAGTGGAAG
GCGCTCAATG AGATCGACGC GGGCGTCTGT GAGGAGCTGA CCTACGAGGA GATCAGGGAC
ACCTACCCTG AGGAGTATGC GCTGCGGGAG CAGGACAAGT ACTATTACCG CTACCCCACC
GGGGAGTCCT ACCAGGACCT GGTCCAGCGC TTGGAGCCAG TGATCATGGA GCTGGAGCGG
CAGGAGAATG TGCTGGTCAT CTGCCACCAG GCCGTCCTGC GCTGCCTGCT TGCCTACTTC
CTGGATAAGA GTGCAGAGGA GATGCCCTAC CTGAAATGCC CTCTTCACAC CGTCCTGAAA
CTGACGCCTG TCGCTTATGG CTGCCGTGTG GAATCCATCT ACCTGAACGT GGAGTCCGTC
TGCACACACC GGGAGAGGTC AGAGGATGCA AAGAAGGGAC CTAACCCGCT CATGAGACGC
AATAGTGTCA CCCCGCTAGC CAGCCCCGAA CCCACCAAAA AGCCTCGCAT CAACAGCTTT
GAGGAGCATG TGGCCTCCAC CTCGGCCGCC CTGCCCAGCT GCCTGCCCCC GGAGGTGCCC
ACGCAGCTGC CTGGACAAAA CATGAAAGGC TCCCGGAGCA GCGCTGACTC CTCCAGGAAA
CACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

PFKFB3 was overexpressed in gastric cancer patients and promoted the proliferation and migration of gastric cancer cells.
Cancer biomarkers : section A of Disease markers18(3)249-256(2017)
Han J,Meng Q,Xi Q,Wang H,Wu G


book

Akt mediates TIGAR induction in HeLa cells following PFKFB3 inhibition.
FEBS letters590(17)2915-26(2016 Sep)
Simon-Molas H,Calvo-Vidal MN,Castaño E,Rodríguez-García A,Navarro-Sabaté À,Bartrons R,Manzano A


book

Targeting of MCT1 and PFKFB3 influences cell proliferation and apoptosis in bladder cancer by altering the tumor microenvironment.
Oncology reports36(2)945-51(2016 Aug)
Hu KY,Wang de G,Liu PF,Cao YW,Wang YH,Yang XC,Hu CX,Sun LJ,Niu HT


book

[EXPRESSION OF PFKFB, HK2, NAMPT, TSPAN13 AND HSPB8 GENES IN PEDIATRIC GLIOMA].
Likars'ka sprava(7-8)43-8(2015 Oct-Dec)
Minchenko DO,Novik YE,Maslak HS,Tiazhka OV,Minchenko OH


book

6-Phosphofructo-2-kinase (PFKFB3) promotes cell cycle progression and suppresses apoptosis via Cdk1-mediated phosphorylation of p27.
Cell death & disease5e1337(2014 Jul)
Yalcin A,Clem BF,Imbert-Fernandez Y,Ozcan SC,Peker S,O'Neal J,Klarer AC,Clem AL,Telang S,Chesney J


 
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