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PLSCR1 cDNA ORF clone, Homo sapiens(human)

Summary

Gene Symbol PLSCR1
Entrez Gene ID 5359
Full Name phospholipid scramblase 1
Synonyms MMTRA1B
General protein information
Preferred Names

phospholipid scramblase 1

Names

phospholipid scramblase 1
PL scramblase 1
ca(2+)-dependent phospholipid scramblase 1
erythrocyte phospholipid scramblase
Gene Type protein-coding
Organism Homo sapiens(human)
Genome

3

3q24
Disorder MIM:

604170


Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_001363874.1 NP_001350803.1 phospholipid scramblase 1 isoform 3
NM_021105.3 NP_066928.1 phospholipid scramblase 1 isoform 1
NM_001363872.1 NP_001350801.1 phospholipid scramblase 1 isoform 2
XM_011512905.1 XP_011511207.1 phospholipid scramblase 1 isoform X3
XM_011512904.1 XP_011511206.1 phospholipid scramblase 1 isoform X2
XM_005247538.3 XP_005247595.1 phospholipid scramblase 1 isoform X4

Pathways from BioSystems


Homologies


Danio rerio (zebrafish) plscr3a NP_001098583.1
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005498 XP_001688666.1
Homo sapiens (human) PLSCR1 NP_066928.1
Rattus norvegicus (Norway rat) Plscr1 NP_476542.1
Drosophila melanogaster (fruit fly) scramb1 NP_648389.3
Anopheles gambiae (African malaria mosquito) AgaP_AGAP005504 XP_001688668.1
Caenorhabditis elegans (roundworm) scrm-3 NP_503934.2
Xenopus tropicalis (tropical clawed frog) LOC100489273 XP_002938452.2
Pan troglodytes (chimpanzee) PLSCR1 XP_001135229.2
PLSCR1 XP_001111222.1
Mus musculus (house mouse) Plscr1 NP_035766.2
Gallus gallus (chicken) PLSCR1 XP_001231237.1
Danio rerio (zebrafish) plscr3b NP_998031.1
Caenorhabditis elegans (roundworm) scrm-2 NP_493321.1
Xenopus tropicalis (tropical clawed frog) plscr1 NP_001135607.1
Canis lupus familiaris (dog) LOC611500 XP_005634707.1
Caenorhabditis elegans (roundworm) scrm-1 NP_492976.2

Gene Ontology


Bibliography

Related articles in PubMed

Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry.
Cheshenko N, Pierce C, Herold BC
PLoS pathogens14(1)e1006766(2018 Jan)

Interactions stabilizing the C-terminal helix of human phospholipid scramblase 1 in lipid bilayers: A computational study.
Venken T, Schillinger AS, Fuglebakk E, Reuter N
Biochimica et biophysica acta1859(7)1200-1210(2017 07)

PLSCR1/IP3R1/Ca
Li H, Xu J, Zhou Y, Liu X, Shen LE, Zhu YU, Li Z, Wang X, Guo Q, Hui H
Cell death & disease8(5)e2768(2017 May)

Phospholipid scramblase 1 as a critical node at the crossroad between autophagy and apoptosis in mantle cell lymphoma.
Mastorci K, Montico B, Faè DA, Sigalotti L, Ponzoni M, Inghirami G, Dolcetti R, Dal Col J
Oncotarget7(27)41913-41928(2016 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

investigate how the polar aspartate residue is accommodated in lipid bilayers containing POPC with and without cholesterol, using all-atom molecular dynamics simulations
Title: Interactions stabilizing the C-terminal helix of human phospholipid scramblase 1 in lipid bilayers: A computational study.

Data indicate heterogeneous expression of phospholipid scramblase 1 (PLSCR1) and suggest its possible implication in the response to anticancer therapies, especially to drugs promoting protective autophagy.
Title: Phospholipid scramblase 1 as a critical node at the crossroad between autophagy and apoptosis in mantle cell lymphoma.

Human phospholipid scramblase 1 has five histidine residues and point mutations of histidine residues to alanine in hPLSCR1 resulted in 60 % loss in nuclease activity.
Title: Identification and characterization of the novel nuclease activity of human phospholipid scramblase 1.

directed mutagenesis revealed the importance of c-Myc binding sites from -751 to -756 and -548 to -553 on the promoter of hPLSCR1in transcriptionally regulating the expression of hPLSCR1.
Title: Two c-Myc binding sites are crucial in upregulating the expression of human phospholipid scramblase 1 gene.

PLSCR1 positively regulates hepatic carcinoma cell proliferation and migration through interacting with midkine
Title: Phospholipid Scramblase 1 Interacts with Midkine and Regulates Hepatic Cancer Cell Proliferation and Migration.

The following PLSCR1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLSCR1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu09066 NM_021105.3
Latest version!		 Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$154.50-$216.30
$309.00
OHu09066 XM_011512904.1
Latest version!		 Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK 		$154.50-$216.30
$309.00
OHu37983 NM_001363874.1
Latest version!		 Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu59784 NM_001363872.1
Latest version!		 Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu37983 XM_005247538.3
Latest version!		 Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu59784 XM_011512905.1
Latest version!		 Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu09066
Clone ID Related Accession (Same CDS sequence) NM_021105.3 , XM_011512904.1
Accession Version NM_021105.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 957bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-06
Organism Homo sapiens(human)
Product phospholipid scramblase 1 isoform 1
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF098642.1 and BC032718.1. On Nov 23, 2018 this sequence version replaced NM_021105.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC032718.1, AF098642.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000342435.9/ ENSP00000345494.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGACAAAC AAAACTCACA GATGAATGCT TCTCACCCGG AAACAAACTT GCCAGTTGGG 
TATCCTCCTC AGTATCCACC GACAGCATTC CAAGGACCTC CAGGATATAG TGGCTACCCT 
GGGCCCCAGG TCAGCTACCC ACCCCCACCA GCCGGCCATT CAGGTCCTGG CCCAGCTGGC 
TTTCCTGTCC CAAATCAGCC AGTGTATAAT CAGCCAGTAT ATAATCAGCC AGTTGGAGCT 
GCAGGGGTAC CATGGATGCC AGCGCCACAG CCTCCATTAA ACTGTCCACC TGGATTAGAA 
TATTTAAGTC AGATAGATCA GATACTGATT CATCAGCAAA TTGAACTTCT GGAAGTTTTA 
ACAGGTTTTG AAACTAATAA CAAATATGAA ATTAAGAACA GCTTTGGACA GAGGGTTTAC 
TTTGCAGCGG AAGATACTGA TTGCTGTACC CGAAATTGCT GTGGGCCATC TAGACCTTTT 
ACCTTGAGGA TTATTGATAA TATGGGTCAA GAAGTCATAA CTCTGGAGAG ACCACTAAGA 
TGTAGCAGCT GTTGTTGTCC CTGCTGCCTT CAGGAGATAG AAATCCAAGC TCCTCCTGGT 
GTACCAATAG GTTATGTTAT TCAGACTTGG CACCCATGTC TACCAAAGTT TACAATTCAA 
AATGAGAAAA GAGAGGATGT ACTAAAAATA AGTGGTCCAT GTGTTGTGTG CAGCTGTTGT 
GGAGATGTTG ATTTTGAGAT TAAATCTCTT GATGAACAGT GTGTGGTTGG CAAAATTTCC 
AAGCACTGGA CTGGAATTTT GAGAGAGGCA TTTACAGACG CTGATAACTT TGGAATCCAG 
TTCCCTTTAG ACCTTGATGT TAAAATGAAA GCTGTAATGA TTGGTGCCTG TTTCCTCATT 
GACTTCATGT TTTTTGAAAG CACTGGCAGC CAGGAACAAA AATCAGGAGT GTGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_066928.1
CDS155..1111
Translation

Target ORF information:

RefSeq Version NM_021105.3
Organism Homo sapiens(human)
Definition Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_021105.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGACAAAC AAAACTCACA GATGAATGCT TCTCACCCGG AAACAAACTT GCCAGTTGGG 
TATCCTCCTC AGTATCCACC GACAGCATTC CAAGGACCTC CAGGATATAG TGGCTACCCT 
GGGCCCCAGG TCAGCTACCC ACCCCCACCA GCCGGCCATT CAGGTCCTGG CCCAGCTGGC 
TTTCCTGTCC CAAATCAGCC AGTGTATAAT CAGCCAGTAT ATAATCAGCC AGTTGGAGCT 
GCAGGGGTAC CATGGATGCC AGCGCCACAG CCTCCATTAA ACTGTCCACC TGGATTAGAA 
TATTTAAGTC AGATAGATCA GATACTGATT CATCAGCAAA TTGAACTTCT GGAAGTTTTA 
ACAGGTTTTG AAACTAATAA CAAATATGAA ATTAAGAACA GCTTTGGACA GAGGGTTTAC 
TTTGCAGCGG AAGATACTGA TTGCTGTACC CGAAATTGCT GTGGGCCATC TAGACCTTTT 
ACCTTGAGGA TTATTGATAA TATGGGTCAA GAAGTCATAA CTCTGGAGAG ACCACTAAGA 
TGTAGCAGCT GTTGTTGTCC CTGCTGCCTT CAGGAGATAG AAATCCAAGC TCCTCCTGGT 
GTACCAATAG GTTATGTTAT TCAGACTTGG CACCCATGTC TACCAAAGTT TACAATTCAA 
AATGAGAAAA GAGAGGATGT ACTAAAAATA AGTGGTCCAT GTGTTGTGTG CAGCTGTTGT 
GGAGATGTTG ATTTTGAGAT TAAATCTCTT GATGAACAGT GTGTGGTTGG CAAAATTTCC 
AAGCACTGGA CTGGAATTTT GAGAGAGGCA TTTACAGACG CTGATAACTT TGGAATCCAG 
TTCCCTTTAG ACCTTGATGT TAAAATGAAA GCTGTAATGA TTGGTGCCTG TTTCCTCATT 
GACTTCATGT TTTTTGAAAG CACTGGCAGC CAGGAACAAA AATCAGGAGT GTGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu09066
Clone ID Related Accession (Same CDS sequence) NM_021105.3 , XM_011512904.1
Accession Version XM_011512904.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 957bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product phospholipid scramblase 1 isoform X2
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGACAAAC AAAACTCACA GATGAATGCT TCTCACCCGG AAACAAACTT GCCAGTTGGG 
TATCCTCCTC AGTATCCACC GACAGCATTC CAAGGACCTC CAGGATATAG TGGCTACCCT 
GGGCCCCAGG TCAGCTACCC ACCCCCACCA GCCGGCCATT CAGGTCCTGG CCCAGCTGGC 
TTTCCTGTCC CAAATCAGCC AGTGTATAAT CAGCCAGTAT ATAATCAGCC AGTTGGAGCT 
GCAGGGGTAC CATGGATGCC AGCGCCACAG CCTCCATTAA ACTGTCCACC TGGATTAGAA 
TATTTAAGTC AGATAGATCA GATACTGATT CATCAGCAAA TTGAACTTCT GGAAGTTTTA 
ACAGGTTTTG AAACTAATAA CAAATATGAA ATTAAGAACA GCTTTGGACA GAGGGTTTAC 
TTTGCAGCGG AAGATACTGA TTGCTGTACC CGAAATTGCT GTGGGCCATC TAGACCTTTT 
ACCTTGAGGA TTATTGATAA TATGGGTCAA GAAGTCATAA CTCTGGAGAG ACCACTAAGA 
TGTAGCAGCT GTTGTTGTCC CTGCTGCCTT CAGGAGATAG AAATCCAAGC TCCTCCTGGT 
GTACCAATAG GTTATGTTAT TCAGACTTGG CACCCATGTC TACCAAAGTT TACAATTCAA 
AATGAGAAAA GAGAGGATGT ACTAAAAATA AGTGGTCCAT GTGTTGTGTG CAGCTGTTGT 
GGAGATGTTG ATTTTGAGAT TAAATCTCTT GATGAACAGT GTGTGGTTGG CAAAATTTCC 
AAGCACTGGA CTGGAATTTT GAGAGAGGCA TTTACAGACG CTGATAACTT TGGAATCCAG 
TTCCCTTTAG ACCTTGATGT TAAAATGAAA GCTGTAATGA TTGGTGCCTG TTTCCTCATT 
GACTTCATGT TTTTTGAAAG CACTGGCAGC CAGGAACAAA AATCAGGAGT GTGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511206.1
CDS204..1160
Translation

Target ORF information:

RefSeq Version XM_011512904.1
Organism Homo sapiens(human)
Definition Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011512904.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGACAAAC AAAACTCACA GATGAATGCT TCTCACCCGG AAACAAACTT GCCAGTTGGG 
TATCCTCCTC AGTATCCACC GACAGCATTC CAAGGACCTC CAGGATATAG TGGCTACCCT 
GGGCCCCAGG TCAGCTACCC ACCCCCACCA GCCGGCCATT CAGGTCCTGG CCCAGCTGGC 
TTTCCTGTCC CAAATCAGCC AGTGTATAAT CAGCCAGTAT ATAATCAGCC AGTTGGAGCT 
GCAGGGGTAC CATGGATGCC AGCGCCACAG CCTCCATTAA ACTGTCCACC TGGATTAGAA 
TATTTAAGTC AGATAGATCA GATACTGATT CATCAGCAAA TTGAACTTCT GGAAGTTTTA 
ACAGGTTTTG AAACTAATAA CAAATATGAA ATTAAGAACA GCTTTGGACA GAGGGTTTAC 
TTTGCAGCGG AAGATACTGA TTGCTGTACC CGAAATTGCT GTGGGCCATC TAGACCTTTT 
ACCTTGAGGA TTATTGATAA TATGGGTCAA GAAGTCATAA CTCTGGAGAG ACCACTAAGA 
TGTAGCAGCT GTTGTTGTCC CTGCTGCCTT CAGGAGATAG AAATCCAAGC TCCTCCTGGT 
GTACCAATAG GTTATGTTAT TCAGACTTGG CACCCATGTC TACCAAAGTT TACAATTCAA 
AATGAGAAAA GAGAGGATGT ACTAAAAATA AGTGGTCCAT GTGTTGTGTG CAGCTGTTGT 
GGAGATGTTG ATTTTGAGAT TAAATCTCTT GATGAACAGT GTGTGGTTGG CAAAATTTCC 
AAGCACTGGA CTGGAATTTT GAGAGAGGCA TTTACAGACG CTGATAACTT TGGAATCCAG 
TTCCCTTTAG ACCTTGATGT TAAAATGAAA GCTGTAATGA TTGGTGCCTG TTTCCTCATT 
GACTTCATGT TTTTTGAAAG CACTGGCAGC CAGGAACAAA AATCAGGAGT GTGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37983
Clone ID Related Accession (Same CDS sequence) NM_001363874.1 , XM_005247538.3
Accession Version NM_001363874.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 714bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-06
Organism Homo sapiens(human)
Product phospholipid scramblase 1 isoform 3
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116544.1 and AC069528.10. On Jun 3, 2018 this sequence version replaced XM_011512907.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.307568.1, SRR1163657.278721.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTTCTCA CCCGGAAACA AACTTGCCAG TTGGGTATCC TCCTCAGTAT CCACCGACAG 
CATTCCAAGA TAGATCAGAT ACTGATTCAT CAGCAAATTG AACTTCTGGA AGTTTTAACA 
GGTTTTGAAA CTAATAACAA ATATGAAATT AAGAACAGCT TTGGACAGAG GGTTTACTTT 
GCAGCGGAAG ATACTGATTG CTGTACCCGA AATTGCTGTG GGCCATCTAG ACCTTTTACC 
TTGAGGATTA TTGATAATAT GGGTCAAGAA GTCATAACTC TGGAGAGACC ACTAAGATGT 
AGCAGCTGTT GTTGTCCCTG CTGCCTTCAG GAGATAGAAA TCCAAGCTCC TCCTGGTGTA 
CCAATAGGTT ATGTTATTCA GACTTGGCAC CCATGTCTAC CAAAGTTTAC AATTCAAAAT 
GAGAAAAGAG AGGATGTACT AAAAATAAGT GGTCCATGTG TTGTGTGCAG CTGTTGTGGA 
GATGTTGATT TTGAGATTAA ATCTCTTGAT GAACAGTGTG TGGTTGGCAA AATTTCCAAG 
CACTGGACTG GAATTTTGAG AGAGGCATTT ACAGACGCTG ATAACTTTGG AATCCAGTTC 
CCTTTAGACC TTGATGTTAA AATGAAAGCT GTAATGATTG GTGCCTGTTT CCTCATTGAC 
TTCATGTTTT TTGAAAGCAC TGGCAGCCAG GAACAAAAAT CAGGAGTGTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001350803.1
CDS142..855
Translation

Target ORF information:

RefSeq Version NM_001363874.1
Organism Homo sapiens(human)
Definition Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001363874.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTTCTCA CCCGGAAACA AACTTGCCAG TTGGGTATCC TCCTCAGTAT CCACCGACAG 
CATTCCAAGA TAGATCAGAT ACTGATTCAT CAGCAAATTG AACTTCTGGA AGTTTTAACA 
GGTTTTGAAA CTAATAACAA ATATGAAATT AAGAACAGCT TTGGACAGAG GGTTTACTTT 
GCAGCGGAAG ATACTGATTG CTGTACCCGA AATTGCTGTG GGCCATCTAG ACCTTTTACC 
TTGAGGATTA TTGATAATAT GGGTCAAGAA GTCATAACTC TGGAGAGACC ACTAAGATGT 
AGCAGCTGTT GTTGTCCCTG CTGCCTTCAG GAGATAGAAA TCCAAGCTCC TCCTGGTGTA 
CCAATAGGTT ATGTTATTCA GACTTGGCAC CCATGTCTAC CAAAGTTTAC AATTCAAAAT 
GAGAAAAGAG AGGATGTACT AAAAATAAGT GGTCCATGTG TTGTGTGCAG CTGTTGTGGA 
GATGTTGATT TTGAGATTAA ATCTCTTGAT GAACAGTGTG TGGTTGGCAA AATTTCCAAG 
CACTGGACTG GAATTTTGAG AGAGGCATTT ACAGACGCTG ATAACTTTGG AATCCAGTTC 
CCTTTAGACC TTGATGTTAA AATGAAAGCT GTAATGATTG GTGCCTGTTT CCTCATTGAC 
TTCATGTTTT TTGAAAGCAC TGGCAGCCAG GAACAAAAAT CAGGAGTGTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59784
Clone ID Related Accession (Same CDS sequence) NM_001363872.1 , XM_011512905.1
Accession Version NM_001363872.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 936bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-06
Organism Homo sapiens(human)
Product phospholipid scramblase 1 isoform 2
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC116544.1 and AC069528.10. On Jun 3, 2018 this sequence version replaced XM_011512905.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.480895.1, SRR1803617.349593.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAATGCTT CTCACCCGGA AACAAACTTG CCAGTTGGGT ATCCTCCTCA GTATCCACCG 
ACAGCATTCC AAGGACCTCC AGGATATAGT GGCTACCCTG GGCCCCAGGT CAGCTACCCA 
CCCCCACCAG CCGGCCATTC AGGTCCTGGC CCAGCTGGCT TTCCTGTCCC AAATCAGCCA 
GTGTATAATC AGCCAGTATA TAATCAGCCA GTTGGAGCTG CAGGGGTACC ATGGATGCCA 
GCGCCACAGC CTCCATTAAA CTGTCCACCT GGATTAGAAT ATTTAAGTCA GATAGATCAG 
ATACTGATTC ATCAGCAAAT TGAACTTCTG GAAGTTTTAA CAGGTTTTGA AACTAATAAC 
AAATATGAAA TTAAGAACAG CTTTGGACAG AGGGTTTACT TTGCAGCGGA AGATACTGAT 
TGCTGTACCC GAAATTGCTG TGGGCCATCT AGACCTTTTA CCTTGAGGAT TATTGATAAT 
ATGGGTCAAG AAGTCATAAC TCTGGAGAGA CCACTAAGAT GTAGCAGCTG TTGTTGTCCC 
TGCTGCCTTC AGGAGATAGA AATCCAAGCT CCTCCTGGTG TACCAATAGG TTATGTTATT 
CAGACTTGGC ACCCATGTCT ACCAAAGTTT ACAATTCAAA ATGAGAAAAG AGAGGATGTA 
CTAAAAATAA GTGGTCCATG TGTTGTGTGC AGCTGTTGTG GAGATGTTGA TTTTGAGATT 
AAATCTCTTG ATGAACAGTG TGTGGTTGGC AAAATTTCCA AGCACTGGAC TGGAATTTTG 
AGAGAGGCAT TTACAGACGC TGATAACTTT GGAATCCAGT TCCCTTTAGA CCTTGATGTT 
AAAATGAAAG CTGTAATGAT TGGTGCCTGT TTCCTCATTG ACTTCATGTT TTTTGAAAGC 
ACTGGCAGCC AGGAACAAAA ATCAGGAGTG TGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001350801.1
CDS98..1033
Translation

Target ORF information:

RefSeq Version NM_001363872.1
Organism Homo sapiens(human)
Definition Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001363872.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAATGCTT CTCACCCGGA AACAAACTTG CCAGTTGGGT ATCCTCCTCA GTATCCACCG 
ACAGCATTCC AAGGACCTCC AGGATATAGT GGCTACCCTG GGCCCCAGGT CAGCTACCCA 
CCCCCACCAG CCGGCCATTC AGGTCCTGGC CCAGCTGGCT TTCCTGTCCC AAATCAGCCA 
GTGTATAATC AGCCAGTATA TAATCAGCCA GTTGGAGCTG CAGGGGTACC ATGGATGCCA 
GCGCCACAGC CTCCATTAAA CTGTCCACCT GGATTAGAAT ATTTAAGTCA GATAGATCAG 
ATACTGATTC ATCAGCAAAT TGAACTTCTG GAAGTTTTAA CAGGTTTTGA AACTAATAAC 
AAATATGAAA TTAAGAACAG CTTTGGACAG AGGGTTTACT TTGCAGCGGA AGATACTGAT 
TGCTGTACCC GAAATTGCTG TGGGCCATCT AGACCTTTTA CCTTGAGGAT TATTGATAAT 
ATGGGTCAAG AAGTCATAAC TCTGGAGAGA CCACTAAGAT GTAGCAGCTG TTGTTGTCCC 
TGCTGCCTTC AGGAGATAGA AATCCAAGCT CCTCCTGGTG TACCAATAGG TTATGTTATT 
CAGACTTGGC ACCCATGTCT ACCAAAGTTT ACAATTCAAA ATGAGAAAAG AGAGGATGTA 
CTAAAAATAA GTGGTCCATG TGTTGTGTGC AGCTGTTGTG GAGATGTTGA TTTTGAGATT 
AAATCTCTTG ATGAACAGTG TGTGGTTGGC AAAATTTCCA AGCACTGGAC TGGAATTTTG 
AGAGAGGCAT TTACAGACGC TGATAACTTT GGAATCCAGT TCCCTTTAGA CCTTGATGTT 
AAAATGAAAG CTGTAATGAT TGGTGCCTGT TTCCTCATTG ACTTCATGTT TTTTGAAAGC 
ACTGGCAGCC AGGAACAAAA ATCAGGAGTG TGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu37983
Clone ID Related Accession (Same CDS sequence) NM_001363874.1 , XM_005247538.3
Accession Version XM_005247538.3 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 714bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-05
Organism Homo sapiens(human)
Product phospholipid scramblase 1 isoform X4
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005247538.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTTCTCA CCCGGAAACA AACTTGCCAG TTGGGTATCC TCCTCAGTAT CCACCGACAG 
CATTCCAAGA TAGATCAGAT ACTGATTCAT CAGCAAATTG AACTTCTGGA AGTTTTAACA 
GGTTTTGAAA CTAATAACAA ATATGAAATT AAGAACAGCT TTGGACAGAG GGTTTACTTT 
GCAGCGGAAG ATACTGATTG CTGTACCCGA AATTGCTGTG GGCCATCTAG ACCTTTTACC 
TTGAGGATTA TTGATAATAT GGGTCAAGAA GTCATAACTC TGGAGAGACC ACTAAGATGT 
AGCAGCTGTT GTTGTCCCTG CTGCCTTCAG GAGATAGAAA TCCAAGCTCC TCCTGGTGTA 
CCAATAGGTT ATGTTATTCA GACTTGGCAC CCATGTCTAC CAAAGTTTAC AATTCAAAAT 
GAGAAAAGAG AGGATGTACT AAAAATAAGT GGTCCATGTG TTGTGTGCAG CTGTTGTGGA 
GATGTTGATT TTGAGATTAA ATCTCTTGAT GAACAGTGTG TGGTTGGCAA AATTTCCAAG 
CACTGGACTG GAATTTTGAG AGAGGCATTT ACAGACGCTG ATAACTTTGG AATCCAGTTC 
CCTTTAGACC TTGATGTTAA AATGAAAGCT GTAATGATTG GTGCCTGTTT CCTCATTGAC 
TTCATGTTTT TTGAAAGCAC TGGCAGCCAG GAACAAAAAT CAGGAGTGTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_005247595.1
CDS378..1091
Translation

Target ORF information:

RefSeq Version XM_005247538.3
Organism Homo sapiens(human)
Definition Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_005247538.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
ATGCTTCTCA CCCGGAAACA AACTTGCCAG TTGGGTATCC TCCTCAGTAT CCACCGACAG 
CATTCCAAGA TAGATCAGAT ACTGATTCAT CAGCAAATTG AACTTCTGGA AGTTTTAACA 
GGTTTTGAAA CTAATAACAA ATATGAAATT AAGAACAGCT TTGGACAGAG GGTTTACTTT 
GCAGCGGAAG ATACTGATTG CTGTACCCGA AATTGCTGTG GGCCATCTAG ACCTTTTACC 
TTGAGGATTA TTGATAATAT GGGTCAAGAA GTCATAACTC TGGAGAGACC ACTAAGATGT 
AGCAGCTGTT GTTGTCCCTG CTGCCTTCAG GAGATAGAAA TCCAAGCTCC TCCTGGTGTA 
CCAATAGGTT ATGTTATTCA GACTTGGCAC CCATGTCTAC CAAAGTTTAC AATTCAAAAT 
GAGAAAAGAG AGGATGTACT AAAAATAAGT GGTCCATGTG TTGTGTGCAG CTGTTGTGGA 
GATGTTGATT TTGAGATTAA ATCTCTTGAT GAACAGTGTG TGGTTGGCAA AATTTCCAAG 
CACTGGACTG GAATTTTGAG AGAGGCATTT ACAGACGCTG ATAACTTTGG AATCCAGTTC 
CCTTTAGACC TTGATGTTAA AATGAAAGCT GTAATGATTG GTGCCTGTTT CCTCATTGAC 
TTCATGTTTT TTGAAAGCAC TGGCAGCCAG GAACAAAAAT CAGGAGTGTG GTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu59784
Clone ID Related Accession (Same CDS sequence) NM_001363872.1 , XM_011512905.1
Accession Version XM_011512905.1 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 936bp)
Protein sequence
SNP
Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-05
Organism Homo sapiens(human)
Product phospholipid scramblase 1 isoform X3
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAATGCTT CTCACCCGGA AACAAACTTG CCAGTTGGGT ATCCTCCTCA GTATCCACCG 
ACAGCATTCC AAGGACCTCC AGGATATAGT GGCTACCCTG GGCCCCAGGT CAGCTACCCA 
CCCCCACCAG CCGGCCATTC AGGTCCTGGC CCAGCTGGCT TTCCTGTCCC AAATCAGCCA 
GTGTATAATC AGCCAGTATA TAATCAGCCA GTTGGAGCTG CAGGGGTACC ATGGATGCCA 
GCGCCACAGC CTCCATTAAA CTGTCCACCT GGATTAGAAT ATTTAAGTCA GATAGATCAG 
ATACTGATTC ATCAGCAAAT TGAACTTCTG GAAGTTTTAA CAGGTTTTGA AACTAATAAC 
AAATATGAAA TTAAGAACAG CTTTGGACAG AGGGTTTACT TTGCAGCGGA AGATACTGAT 
TGCTGTACCC GAAATTGCTG TGGGCCATCT AGACCTTTTA CCTTGAGGAT TATTGATAAT 
ATGGGTCAAG AAGTCATAAC TCTGGAGAGA CCACTAAGAT GTAGCAGCTG TTGTTGTCCC 
TGCTGCCTTC AGGAGATAGA AATCCAAGCT CCTCCTGGTG TACCAATAGG TTATGTTATT 
CAGACTTGGC ACCCATGTCT ACCAAAGTTT ACAATTCAAA ATGAGAAAAG AGAGGATGTA 
CTAAAAATAA GTGGTCCATG TGTTGTGTGC AGCTGTTGTG GAGATGTTGA TTTTGAGATT 
AAATCTCTTG ATGAACAGTG TGTGGTTGGC AAAATTTCCA AGCACTGGAC TGGAATTTTG 
AGAGAGGCAT TTACAGACGC TGATAACTTT GGAATCCAGT TCCCTTTAGA CCTTGATGTT 
AAAATGAAAG CTGTAATGAT TGGTGCCTGT TTCCTCATTG ACTTCATGTT TTTTGAAAGC 
ACTGGCAGCC AGGAACAAAA ATCAGGAGTG TGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511207.1
CDS280..1215
Translation

Target ORF information:

RefSeq Version XM_011512905.1
Organism Homo sapiens(human)
Definition Homo sapiens phospholipid scramblase 1 (PLSCR1), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011512905.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGAATGCTT CTCACCCGGA AACAAACTTG CCAGTTGGGT ATCCTCCTCA GTATCCACCG 
ACAGCATTCC AAGGACCTCC AGGATATAGT GGCTACCCTG GGCCCCAGGT CAGCTACCCA 
CCCCCACCAG CCGGCCATTC AGGTCCTGGC CCAGCTGGCT TTCCTGTCCC AAATCAGCCA 
GTGTATAATC AGCCAGTATA TAATCAGCCA GTTGGAGCTG CAGGGGTACC ATGGATGCCA 
GCGCCACAGC CTCCATTAAA CTGTCCACCT GGATTAGAAT ATTTAAGTCA GATAGATCAG 
ATACTGATTC ATCAGCAAAT TGAACTTCTG GAAGTTTTAA CAGGTTTTGA AACTAATAAC 
AAATATGAAA TTAAGAACAG CTTTGGACAG AGGGTTTACT TTGCAGCGGA AGATACTGAT 
TGCTGTACCC GAAATTGCTG TGGGCCATCT AGACCTTTTA CCTTGAGGAT TATTGATAAT 
ATGGGTCAAG AAGTCATAAC TCTGGAGAGA CCACTAAGAT GTAGCAGCTG TTGTTGTCCC 
TGCTGCCTTC AGGAGATAGA AATCCAAGCT CCTCCTGGTG TACCAATAGG TTATGTTATT 
CAGACTTGGC ACCCATGTCT ACCAAAGTTT ACAATTCAAA ATGAGAAAAG AGAGGATGTA 
CTAAAAATAA GTGGTCCATG TGTTGTGTGC AGCTGTTGTG GAGATGTTGA TTTTGAGATT 
AAATCTCTTG ATGAACAGTG TGTGGTTGGC AAAATTTCCA AGCACTGGAC TGGAATTTTG 
AGAGAGGCAT TTACAGACGC TGATAACTTT GGAATCCAGT TCCCTTTAGA CCTTGATGTT 
AAAATGAAAG CTGTAATGAT TGGTGCCTGT TTCCTCATTG ACTTCATGTT TTTTGAAAGC 
ACTGGCAGCC AGGAACAAAA ATCAGGAGTG TGGTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry.
PLoS pathogens14(1)e1006766(2018 Jan)
Cheshenko N,Pierce C,Herold BC


book

Interactions stabilizing the C-terminal helix of human phospholipid scramblase 1 in lipid bilayers: A computational study.
Biochimica et biophysica acta1859(7)1200-1210(2017 07)
Venken T,Schillinger AS,Fuglebakk E,Reuter N


book

PLSCR1/IP3R1/Ca
Cell death & disease8(5)e2768(2017 May)
Li H,Xu J,Zhou Y,Liu X,Shen LE,Zhu YU,Li Z,Wang X,Guo Q,Hui H


book

Phospholipid scramblase 1 as a critical node at the crossroad between autophagy and apoptosis in mantle cell lymphoma.
Oncotarget7(27)41913-41928(2016 Jul)
Mastorci K,Montico B,Faè DA,Sigalotti L,Ponzoni M,Inghirami G,Dolcetti R,Dal Col J