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THPO cDNA ORF clone, Homo sapiens (human)

Gene Symbol THPO
Entrez Gene ID 7066
Full Name thrombopoietin
Synonyms MGDF, MKCSF, ML, MPLLG, THCYT1, TPO
General protein information
Preferred Names
thrombopoietin
Names
thrombopoietin
MPL ligand
c-mpl ligand
thrombopoietin nirs variant 1
megakaryocyte stimulating factor
megakaryocyte colony-stimulating factor
megakaryocyte growth and development factor
myeloproliferative leukemia virus oncogene ligand
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

3

3q27

Summary Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]. lac of sum
Disorder MIM:

600044

Disorder Html: Thrombocythemia, essential, 187950 (3)

mRNA and Protein(s)

mRNA Protein Name
NM_001177598 NP_001171069 thrombopoietin isoform 3 precursor
NM_001290026 NP_001276955 thrombopoietin isoform 3 precursor
XM_011513113 XP_011511415 thrombopoietin isoform X1
NM_000460 NP_000451 thrombopoietin isoform 1 precursor
NM_001177597 NP_001171068 thrombopoietin isoform 2 precursor
NM_001290003 NP_001276932 thrombopoietin isoform 5
NM_001289998 NP_001276927 thrombopoietin isoform 1 precursor
NM_001290022 NP_001276951 thrombopoietin isoform 2 precursor
NM_001289997 NP_001276926 thrombopoietin isoform 4 precursor
NM_001290027 NP_001276956 thrombopoietin isoform 4 precursor
NM_001290028 NP_001276957 thrombopoietin isoform 1 precursor



Homo sapiens (human) THPO NP_000451.1
Pan troglodytes (chimpanzee) THPO XP_001136518.2
Canis lupus familiaris (dog) THPO XP_005639837.1
Bos taurus (cattle) THPO NP_001159512.1
Mus musculus (house mouse) Thpo NP_033405.1
Rattus norvegicus (Norway rat) LOC100910397 XP_006248642.1
Rattus norvegicus (Norway rat) Thpo NP_112395.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following THPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu02046 NM_001177598 Homo sapiens thrombopoietin (THPO), transcript variant 3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu02046 NM_001290026 Homo sapiens thrombopoietin (THPO), transcript variant 7, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu60994 XM_011513113 PREDICTED: Homo sapiens thrombopoietin (THPO), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu10732
NM_000460 Homo sapiens thrombopoietin (THPO), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu02053
NM_001177597 Homo sapiens thrombopoietin (THPO), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu11385 NM_001290003 Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu10732
NM_001289998 Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu02053
NM_001290022 Homo sapiens thrombopoietin (THPO), transcript variant 6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00
OHu02030 NM_001289997 Homo sapiens thrombopoietin (THPO), transcript variant 4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu02030 NM_001290027 Homo sapiens thrombopoietin (THPO), transcript variant 8, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $139.30
$199.00
OHu10732
NM_001290028 Homo sapiens thrombopoietin (THPO), transcript variant 9, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$159.50-$223.30
$319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu02046
Clone ID Related Accession (Same CDS sequence) NM_001177598 , NM_001290026
Accession Version NM_001177598.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 960bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 16-MAR-2014
Organism Homo sapiens (human)
Product thrombopoietin isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AB102890.1, AA480029.1 and AA479058.1. On Feb 21, 2014 this sequence version replaced gi:294862251. Transcript Variant: This variant (3) represents use of the downstream promoter and comprises six exons. It uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB102890.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001171069.1
CDS280..1239
Misc Feature(1)1..1(+)
Misc Feature(2)21..21(+)
Misc Feature(3)23..139(+)
Misc Feature(4)28..28(+)
Misc Feature(5)31..31(+)
Misc Feature(6)44..44(+)
Misc Feature(7)45..45(+)
Misc Feature(8)62..139(+)
Misc Feature(9)71..71(+)
Misc Feature(10)210..293(+)
Misc Feature(11)349..795(+)
Exon (1)1..134
Gene:THPO
Gene Synonym:
Exon (2)135..292
Gene:THPO
Gene Synonym:
Exon (3)293..420
Gene:THPO
Gene Synonym:
Exon (4)421..507
Gene:THPO
Gene Synonym:
Exon (5)508..675
Gene:THPO
Gene Synonym:
Exon (6)676..1902
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001177598
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177598

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02046
Clone ID Related Accession (Same CDS sequence) NM_001177598 , NM_001290026
Accession Version NM_001290026.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 960bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 05-NOV-2014
Organism Homo sapiens (human)
Product thrombopoietin isoform 3 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AB102890.1, AA480029.1 and AA479058.1. Transcript Variant: This variant (7) represents use of the upstream promoter and comprises seven exons. This variant is longer at the 5' end and has an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. This variant includes two main in-frame AUG sites, but translation initiated from the upstream AUG codon for this variant is not reported in literature. The isoform (3) represented in this RefSeq is derived from the downstream AUG start codon; it is identical to the isoform encoded by variant 3 and lacks an internal segment, as compared to isoform 1. This variant was reported in PMID: 7822271. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB102890.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276955.1
CDS547..1506
Misc Feature(1)1..1(+)
Misc Feature(2)60..77(+)
Misc Feature(3)61..63(+)
Misc Feature(4)80..262(+)
Misc Feature(5)113..262(+)
Misc Feature(6)127..129(+)
Misc Feature(7)268..268(+)
Misc Feature(8)288..288(+)
Misc Feature(9)290..406(+)
Misc Feature(10)295..295(+)
Misc Feature(11)298..298(+)
Misc Feature(12)311..311(+)
Misc Feature(13)312..312(+)
Misc Feature(14)329..406(+)
Misc Feature(15)338..338(+)
Misc Feature(16)477..560(+)
Misc Feature(17)616..1062(+)
Exon (1)1..98
Gene:THPO
Gene Synonym:
Exon (2)99..401
Gene:THPO
Gene Synonym:
Exon (3)402..559
Gene:THPO
Gene Synonym:
Exon (4)560..687
Gene:THPO
Gene Synonym:
Exon (5)688..774
Gene:THPO
Gene Synonym:
Exon (6)775..942
Gene:THPO
Gene Synonym:
Exon (7)943..2169
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001290026
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 7, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001290026

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu60994
Clone ID Related Accession (Same CDS sequence) XM_011513113
Accession Version XM_011513113.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_005612.17) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGACTT CTGGATTGTT
GGAGACAAAC TTCACTGCCT CAGCCAGAAC TACTGGCTCT GGGCTTCTGA AGTGGCAGCA
GGGATTCAGA GCCAAGATTC CTGGTCTGCT GAACCAAACC TCCAGGTCCC TGGACCAAAT
CCCCGGATAC CTGAACAGGA TACACGAACT CTTGAATGGA ACTCGTGGAC TCTTTCCTGG
ACCCTCACGC AGGACCCTAG GAGCCCCGGA CATTTCCTCA GGAACATCAG ACACAGGCTC
CCTGCCACCC AACCTCCAGC CTGGATATTC TCCTTCCCCA ACCCATCCTC CTACTGGACA
GTATACGCTC TTCCCTCTTC CACCCACCTT GCCCACCCCT GTGGTCCAGC TCCACCCCCT
GCTTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011511415.1
CDS23..1291
Misc Feature(1)512..907(+)
Translation

Target ORF information:

RefSeq Version XM_011513113
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens thrombopoietin (THPO), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011513113

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGACTT CTGGATTGTT
GGAGACAAAC TTCACTGCCT CAGCCAGAAC TACTGGCTCT GGGCTTCTGA AGTGGCAGCA
GGGATTCAGA GCCAAGATTC CTGGTCTGCT GAACCAAACC TCCAGGTCCC TGGACCAAAT
CCCCGGATAC CTGAACAGGA TACACGAACT CTTGAATGGA ACTCGTGGAC TCTTTCCTGG
ACCCTCACGC AGGACCCTAG GAGCCCCGGA CATTTCCTCA GGAACATCAG ACACAGGCTC
CCTGCCACCC AACCTCCAGC CTGGATATTC TCCTTCCCCA ACCCATCCTC CTACTGGACA
GTATACGCTC TTCCCTCTTC CACCCACCTT GCCCACCCCT GTGGTCCAGC TCCACCCCCT
GCTTCCTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10732
Clone ID Related Accession (Same CDS sequence) NM_000460 , NM_001289998 , NM_001290028
Accession Version NM_000460.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. On Feb 21, 2014 this sequence version replaced gi:40805871. Transcript Variant: This variant (1) represents use of the downstream promoter and comprises six exons. It encodes the main functional protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: L33410.1, BC136338.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_000451.1
CDS280..1341
Misc Feature(1)1..1(+)
Misc Feature(2)21..21(+)
Misc Feature(3)23..139(+)
Misc Feature(4)28..28(+)
Misc Feature(5)31..31(+)
Misc Feature(6)44..44(+)
Misc Feature(7)45..45(+)
Misc Feature(8)62..139(+)
Misc Feature(9)71..71(+)
Misc Feature(10)210..293(+)
Misc Feature(11)349..843(+)
Misc Feature(12)361..795(+)
Misc Feature(13)427..597(+)
Misc Feature(14)451..453(+)
Misc Feature(15)670..672(+)
Misc Feature(16)814..816(+)
Misc Feature(17)817..819(+)
Misc Feature(18)829..831(+)
Misc Feature(19)868..870(+)
Misc Feature(20)895..897(+)
Misc Feature(21)916..918(+)
Misc Feature(22)979..981(+)
Misc Feature(23)1042..1044(+)
Misc Feature(24)1072..1074(+)
Misc Feature(25)1297..1299(+)
Misc Feature(26)1321..1323(+)
Exon (1)1..134
Gene:THPO
Gene Synonym:
Exon (2)135..292
Gene:THPO
Gene Synonym:
Exon (3)293..420
Gene:THPO
Gene Synonym:
Exon (4)421..507
Gene:THPO
Gene Synonym:
Exon (5)508..675
Gene:THPO
Gene Synonym:
Exon (6)676..1919
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_000460
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_000460

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02053
Clone ID Related Accession (Same CDS sequence) NM_001177597 , NM_001290022
Accession Version NM_001177597.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1050bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, L33410.1 and AA479058.1. On Feb 21, 2014 this sequence version replaced gi:294862249. Transcript Variant: This variant (2) represents use of the downstream promoter and comprises six exons. It uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## ##Evidence-Data-START## Transcript exon combination :: BC130322.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001171068.1
CDS280..1329
Misc Feature(1)1..1(+)
Misc Feature(2)21..21(+)
Misc Feature(3)23..139(+)
Misc Feature(4)28..28(+)
Misc Feature(5)31..31(+)
Misc Feature(6)44..44(+)
Misc Feature(7)45..45(+)
Misc Feature(8)62..139(+)
Misc Feature(9)71..71(+)
Misc Feature(10)210..293(+)
Misc Feature(11)349..831(+)
Exon (1)1..134
Gene:THPO
Gene Synonym:
Exon (2)135..292
Gene:THPO
Gene Synonym:
Exon (3)293..420
Gene:THPO
Gene Synonym:
Exon (4)421..507
Gene:THPO
Gene Synonym:
Exon (5)508..675
Gene:THPO
Gene Synonym:
Exon (6)676..1907
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001177597
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001177597

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu11385
Clone ID Related Accession (Same CDS sequence) NM_001290003
Accession Version NM_001290003.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 5
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. Transcript Variant: This variant (5) represents use of the upstream promoter and comprises seven exons. It is longer at the 5' end, compared to variant 1. This variant can initiate translation from two in-frame AUG sites. The isoform (5) represented in this Refseq is derived from the upstream AUG start codon and is longer at the N-terminus, compared to isoform 1. This isoform was described as a uAUG4 product in PMID: 9834204 and had no thrombopoietin bioactivity. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276932.1
CDS127..1608
Misc Feature(1)1..1(+)
Misc Feature(2)60..77(+)
Misc Feature(3)61..63(+)
Misc Feature(4)80..262(+)
Misc Feature(5)113..262(+)
Misc Feature(6)616..1110(+)
Exon (1)1..98
Gene:THPO
Gene Synonym:
Exon (2)99..401
Gene:THPO
Gene Synonym:
Exon (3)402..559
Gene:THPO
Gene Synonym:
Exon (4)560..687
Gene:THPO
Gene Synonym:
Exon (5)688..774
Gene:THPO
Gene Synonym:
Exon (6)775..942
Gene:THPO
Gene Synonym:
Exon (7)943..2186
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001290003
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001290003

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10732
Clone ID Related Accession (Same CDS sequence) NM_000460 , NM_001289998 , NM_001290028
Accession Version NM_001289998.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. Transcript Variant: This variant (5) represents use of the upstream promoter and comprises seven exons. It is longer at the 5' end, compared to variant 1. This variant can initiate translation from two in-frame AUG sites. The isoform (1) represented in this Refseq is derived from the downstream AUG start codon and is identical to the isoform encoded by variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L33410.1, D32047.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276927.1
CDS547..1608
Misc Feature(1)1..1(+)
Misc Feature(2)60..77(+)
Misc Feature(3)61..63(+)
Misc Feature(4)80..262(+)
Misc Feature(5)113..262(+)
Misc Feature(6)127..129(+)
Misc Feature(7)268..268(+)
Misc Feature(8)288..288(+)
Misc Feature(9)290..406(+)
Misc Feature(10)295..295(+)
Misc Feature(11)298..298(+)
Misc Feature(12)311..312(+)
Misc Feature(13)329..406(+)
Misc Feature(14)338..338(+)
Misc Feature(15)477..560(+)
Misc Feature(16)616..1110(+)
Exon (1)1..98
Gene:THPO
Gene Synonym:
Exon (2)99..401
Gene:THPO
Gene Synonym:
Exon (3)402..559
Gene:THPO
Gene Synonym:
Exon (4)560..687
Gene:THPO
Gene Synonym:
Exon (5)688..774
Gene:THPO
Gene Synonym:
Exon (6)775..942
Gene:THPO
Gene Synonym:
Exon (7)943..2186
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001289998
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289998

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02053
Clone ID Related Accession (Same CDS sequence) NM_001177597 , NM_001290022
Accession Version NM_001290022.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1050bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 2 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, L33410.1 and AA479058.1. Transcript Variant: This variant (6) represents use of the upstream promoter and comprises seven exons. This variant is longer at the 5' end and has an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant includes two main in-frame AUG sites, but translation initiated from the upstream AUG codon for this variant is not reported in literature. The isoform (2) represented in this RefSeq is derived from the downstream AUG start codon; it is identical to the isoform encoded by variant 2 and lacks an internal segment, as compared to isoform 1. This variant was reported in PMID: 7822271. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC130322.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276951.1
CDS547..1596
Misc Feature(1)1..1(+)
Misc Feature(2)60..77(+)
Misc Feature(3)61..63(+)
Misc Feature(4)80..262(+)
Misc Feature(5)113..262(+)
Misc Feature(6)127..129(+)
Misc Feature(7)268..268(+)
Misc Feature(8)288..288(+)
Misc Feature(9)290..406(+)
Misc Feature(10)295..295(+)
Misc Feature(11)298..298(+)
Misc Feature(12)311..311(+)
Misc Feature(13)312..312(+)
Misc Feature(14)329..406(+)
Misc Feature(15)338..338(+)
Misc Feature(16)477..560(+)
Misc Feature(17)616..1098(+)
Exon (1)1..98
Gene:THPO
Gene Synonym:
Exon (2)99..401
Gene:THPO
Gene Synonym:
Exon (3)402..559
Gene:THPO
Gene Synonym:
Exon (4)560..687
Gene:THPO
Gene Synonym:
Exon (5)688..774
Gene:THPO
Gene Synonym:
Exon (6)775..942
Gene:THPO
Gene Synonym:
Exon (7)943..2174
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001290022
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001290022

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02030
Clone ID Related Accession (Same CDS sequence) NM_001289997 , NM_001290027
Accession Version NM_001289997.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 861bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AK311002.1, U11025.1, L33410.1 and AA479058.1. Transcript Variant: This variant (4) represents use of the downstream promoter and comprises six exons. It lacks an internal segment in the 3' exon, which results in a frameshift, compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
GCTCCACCCC CTGCTTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276926.1
CDS280..1140
Misc Feature(1)1..1(+)
Misc Feature(2)21..21(+)
Misc Feature(3)23..139(+)
Misc Feature(4)28..28(+)
Misc Feature(5)31..31(+)
Misc Feature(6)44..44(+)
Misc Feature(7)45..45(+)
Misc Feature(8)62..139(+)
Misc Feature(9)71..71(+)
Misc Feature(10)210..293(+)
Misc Feature(11)349..756(+)
Exon (1)1..134
Gene:THPO
Gene Synonym:
Exon (2)135..292
Gene:THPO
Gene Synonym:
Exon (3)293..420
Gene:THPO
Gene Synonym:
Exon (4)421..507
Gene:THPO
Gene Synonym:
Exon (5)508..675
Gene:THPO
Gene Synonym:
Exon (6)676..756
Gene:THPO
Gene Synonym:
Exon (7)757..1803
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001289997
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001289997

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
GCTCCACCCC CTGCTTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu02030
Clone ID Related Accession (Same CDS sequence) NM_001289997 , NM_001290027
Accession Version NM_001290027.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 861bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 4 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AK311002.1, U11025.1, L33410.1 and AA479058.1. Transcript Variant: This variant (8) represents use of the upstream promoter and comprises seven exons. It includes two main in-frame AUG sites, but translation initiated from the upstream AUG codon for this variant is not reported in literature. The isoform (4) represented in this RefSeq is derived from the downstream AUG start codon; it is identical to the isoform encoded by variant 4 and lacks an internal segment, as compared to isoform 1. This variant was reported in PMID: 7822271. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
GCTCCACCCC CTGCTTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276956.1
CDS547..1407
Misc Feature(1)1..1(+)
Misc Feature(2)60..77(+)
Misc Feature(3)61..63(+)
Misc Feature(4)80..262(+)
Misc Feature(5)113..262(+)
Misc Feature(6)127..129(+)
Misc Feature(7)268..268(+)
Misc Feature(8)288..288(+)
Misc Feature(9)290..407(+)
Misc Feature(10)295..295(+)
Misc Feature(11)298..298(+)
Misc Feature(12)311..311(+)
Misc Feature(13)312..312(+)
Misc Feature(14)329..407(+)
Misc Feature(15)338..338(+)
Misc Feature(16)477..560(+)
Misc Feature(17)616..1023(+)
Exon (1)1..98
Gene:THPO
Gene Synonym:
Exon (2)99..401
Gene:THPO
Gene Synonym:
Exon (3)402..559
Gene:THPO
Gene Synonym:
Exon (4)560..687
Gene:THPO
Gene Synonym:
Exon (5)688..774
Gene:THPO
Gene Synonym:
Exon (6)775..942
Gene:THPO
Gene Synonym:
Exon (7)943..1023
Gene:THPO
Gene Synonym:
Exon (8)1024..2070
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001290027
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 8, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001290027

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
GCTCCACCCC CTGCTTCCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu10732
Clone ID Related Accession (Same CDS sequence) NM_000460 , NM_001289998 , NM_001290028
Accession Version NM_001290028.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 13-MAY-2015
Organism Homo sapiens (human)
Product thrombopoietin isoform 1 precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. Transcript Variant: This variant (9) represents use of the upstream promoter, but lacks an internal exon, compared to other transcripts with the same upstream promoter usage. This variant has an alternate 5' UTR exon and encodes the same isoform (1), compared to variant 1. This variant was reported in PMID: 9834204. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L33410.1, D32047.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001276957.1
CDS244..1305
Misc Feature(1)1..1(+)
Misc Feature(2)60..77(+)
Misc Feature(3)61..63(+)
Misc Feature(4)80..103(+)
Misc Feature(5)174..257(+)
Misc Feature(6)313..807(+)
Exon (1)1..98
Gene:THPO
Gene Synonym:
Exon (2)99..256
Gene:THPO
Gene Synonym:
Exon (3)257..384
Gene:THPO
Gene Synonym:
Exon (4)385..471
Gene:THPO
Gene Synonym:
Exon (5)472..639
Gene:THPO
Gene Synonym:
Exon (6)640..1883
Gene:THPO
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001290028
Organism Homo sapiens (human)
Definition Homo sapiens thrombopoietin (THPO), transcript variant 9, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001290028

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Exome sequencing reveals a thrombopoietin ligand mutation in a Micronesian family with autosomal recessive aplastic anemia
Blood 122 (20), 3440-3449 (2013)
Dasouki MJ, Rafi SK, Olm-Shipman AJ, Wilson NR, Abhyankar S, Ganter B, Furness LM, Fang J, Calado RT and Saadi I.


book

Functional and structural profiling of the human thrombopoietin gene promoter
J. Biol. Chem. 283 (36), 24382-24391 (2008)
Dordelmann C, Telgmann R, Brand E, Hagedorn C, Schroer B, Hasenkamp S, Baumgart P, Kleine-Katthofer P, Paul M and Brand-Herrmann SM.


book

Thrombopoietin production is inhibited by a translational mechanism
Blood 92 (11), 4023-4030 (1998)
Ghilardi N, Wiestner A and Skoda RC.


book

An activating splice donor mutation in the thrombopoietin gene causes hereditary thrombocythaemia
Nat. Genet. 18 (1), 49-52 (1998)
Wiestner A, Schlemper RJ, van der Maas AP and Skoda RC.


book

Characterization of the human thrombopoietin gene promoter. A possible role of an Ets transcription factor, E4TF1/GABP
J. Biol. Chem. 272 (17), 11361-11368 (1997)
Kamura T, Handa H, Hamasaki N and Kitajima S.


book

Genomic structure, chromosomal localization, and conserved alternative splice forms of thrombopoietin
Blood 85 (4), 981-988 (1995)
Gurney AL, Kuang WJ, Xie MH, Malloy BE, Eaton DL and de Sauvage FJ.


book

Cloning and characterization of the human megakaryocyte growth and development factor (MGDF) gene
J. Biol. Chem. 270 (2), 511-514 (1995)
Chang MS, McNinch J, Basu R, Shutter J, Hsu RY, Perkins C, Mar V, Suggs S, Welcher A and Li L.


book

Human thrombopoietin: gene structure, cDNA sequence, expression, and chromosomal localization
Proc. Natl. Acad. Sci. U.S.A. 91 (26), 13023-13027 (1994)
Foster DC, Sprecher CA, Grant FJ, Kramer JM, Kuijper JL, Holly RD, Whitmore TE, Heipel MD, Bell LA and Ching AF.


book

Molecular cloning and chromosomal localization of the human thrombopoietin gene
FEBS Lett. 353 (1), 57-61 (1994)
Sohma Y, Akahori H, Seki N, Hori T, Ogami K, Kato T, Shimada Y, Kawamura K and Miyazaki H.


book

Identification and cloning of a megakaryocyte growth and development factor that is a ligand for the cytokine receptor Mpl
Cell 77 (7), 1117-1124 (1994)
Bartley TD, Bogenberger J, Hunt P, Li YS, Lu HS, Martin F, Chang MS, Samal B, Nichol JL and Swift S.


 
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