THPO cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following THPO gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the THPO cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu02053 NM_001290022.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu02053 NM_001177597.2
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu10732 NM_000460.4
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu10732 NM_001289998.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu10732 NM_001290028.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu02030 NM_001290027.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu02030 NM_001289997.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu02046 NM_001290026.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu02046 NM_001177598.2
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu11385 NM_001290003.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu60994 XM_017007107.1
Latest version!
Homo sapiens thrombopoietin (THPO), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
View Old Accession Versions >>
OHu10732 NM_000460.3 Homo sapiens thrombopoietin (THPO), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu02053
    Clone ID Related Accession (Same CDS sequence) NM_001177597.2 , NM_001290022.1
    Accession Version NM_001177597.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, L33410.1 and AA479058.1. On Feb 21, 2014 this sequence version replaced NM_001177597.1. Transcript Variant: This variant (2) represents use of the downstream promoter and comprises six exons. It uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC130322.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
    CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
    CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
    AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
    ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
    TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
    GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
    TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
    CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
    ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
    CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
    CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171068.1
    CDS280..1329
    Translation

    Target ORF information:

    RefSeq Version NM_001177597.2
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177597.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
    CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
    CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
    AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
    ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
    TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
    GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
    TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
    CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
    ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
    CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
    CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02053
    Clone ID Related Accession (Same CDS sequence) NM_001177597.2 , NM_001290022.1
    Accession Version NM_001290022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1050bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, L33410.1 and AA479058.1. Transcript Variant: This variant (6) represents use of the upstream promoter and comprises seven exons. This variant is longer at the 5' end and has an alternate in-frame splice site in the 3' coding region, compared to variant 1. This variant includes two main in-frame AUG sites, but translation initiated from the upstream AUG codon for this variant is not reported in literature. The isoform (2) represented in this RefSeq is derived from the downstream AUG start codon; it is identical to the isoform encoded by variant 2 and lacks an internal segment, as compared to isoform 1. This variant was reported in PMID: 7822271. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC130322.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
    CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
    CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
    AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
    ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
    TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
    GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
    TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
    CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
    ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
    CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
    CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276951.1
    CDS547..1596
    Translation

    Target ORF information:

    RefSeq Version NM_001290022.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
    CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGTGCG TTTCCTGATG
    CTTGTAGGAG GGTCCACCCT CTGCGTCAGG CGGGCCCCAC CCACCACAGC TGTCCCCAGC
    AGAACCTCTC TAGTCCTCAC ACTGAACGAG CTCCCAAACA GGACTTCTGG ATTGTTGGAG
    ACAAACTTCA CTGCCTCAGC CAGAACTACT GGCTCTGGGC TTCTGAAGTG GCAGCAGGGA
    TTCAGAGCCA AGATTCCTGG TCTGCTGAAC CAAACCTCCA GGTCCCTGGA CCAAATCCCC
    GGATACCTGA ACAGGATACA CGAACTCTTG AATGGAACTC GTGGACTCTT TCCTGGACCC
    TCACGCAGGA CCCTAGGAGC CCCGGACATT TCCTCAGGAA CATCAGACAC AGGCTCCCTG
    CCACCCAACC TCCAGCCTGG ATATTCTCCT TCCCCAACCC ATCCTCCTAC TGGACAGTAT
    ACGCTCTTCC CTCTTCCACC CACCTTGCCC ACCCCTGTGG TCCAGCTCCA CCCCCTGCTT
    CCTGACCCTT CTGCTCCAAC GCCCACCCCT ACCAGCCCTC TTCTAAACAC ATCCTACACC
    CACTCCCAGA ATCTGTCTCA GGAAGGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10732
    Clone ID Related Accession (Same CDS sequence) NM_001289998.1 , NM_001290028.1 , NM_000460.3 , NM_000460.4
    Accession Version NM_001289998.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. Transcript Variant: This variant (5) represents use of the upstream promoter and comprises seven exons. It is longer at the 5' end, compared to variant 1. This variant can initiate translation from two in-frame AUG sites. The isoform (1) represented in this Refseq is derived from the downstream AUG start codon and is identical to the isoform encoded by variant 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L33410.1, D32047.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276927.1
    CDS547..1608
    Translation

    Target ORF information:

    RefSeq Version NM_001289998.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289998.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10732
    Clone ID Related Accession (Same CDS sequence) NM_001289998.1 , NM_001290028.1 , NM_000460.3 , NM_000460.4
    Accession Version NM_001290028.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. Transcript Variant: This variant (9) represents use of the upstream promoter, but lacks an internal exon, compared to other transcripts with the same upstream promoter usage. This variant has an alternate 5' UTR exon and encodes the same isoform (1), compared to variant 1. This variant was reported in PMID: 9834204. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: L33410.1, D32047.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276957.1
    CDS244..1305
    Translation

    Target ORF information:

    RefSeq Version NM_001290028.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290028.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10732
    Clone ID Related Accession (Same CDS sequence) NM_001289998.1 , NM_001290028.1 , NM_000460.3 , NM_000460.4
    Accession Version NM_000460.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. This sequence is a reference standard in the RefSeqGene project. On Feb 21, 2014 this sequence version replaced NM_000460.2. Transcript Variant: This variant (1) represents use of the downstream promoter and comprises six exons. It encodes the main functional protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L33410.1, BC136338.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000451.1
    CDS280..1341
    Translation

    Target ORF information:

    RefSeq Version NM_000460.3
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000460.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu10732
    Clone ID Related Accession (Same CDS sequence) NM_001289998.1 , NM_001290028.1 , NM_000460.3 , NM_000460.4
    Accession Version NM_000460.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1062bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. This sequence is a reference standard in the RefSeqGene project. On May 9, 2018 this sequence version replaced NM_000460.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L33410.1, SRR5189664.158503.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_000451.1
    CDS279..1340
    Translation

    Target ORF information:

    RefSeq Version NM_000460.4
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_000460.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02030
    Clone ID Related Accession (Same CDS sequence) NM_001289997.1 , NM_001290027.1
    Accession Version NM_001289997.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AK311002.1, U11025.1, L33410.1 and AA479058.1. Transcript Variant: This variant (4) represents use of the downstream promoter and comprises six exons. It lacks an internal segment in the 3' exon, which results in a frameshift, compared to variant 1. The resulting isoform (4) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
    TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
    GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
    CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
    ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
    AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
    TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
    GCTCCACCCC CTGCTTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276926.1
    CDS280..1140
    Translation

    Target ORF information:

    RefSeq Version NM_001289997.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001289997.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
    TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
    GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
    CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
    ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
    AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
    TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
    GCTCCACCCC CTGCTTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02030
    Clone ID Related Accession (Same CDS sequence) NM_001289997.1 , NM_001290027.1
    Accession Version NM_001290027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 4 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AK311002.1, U11025.1, L33410.1 and AA479058.1. Transcript Variant: This variant (8) represents use of the upstream promoter and comprises seven exons. It includes two main in-frame AUG sites, but translation initiated from the upstream AUG codon for this variant is not reported in literature. The isoform (4) represented in this RefSeq is derived from the downstream AUG start codon; it is identical to the isoform encoded by variant 4 and lacks an internal segment, as compared to isoform 1. This variant was reported in PMID: 7822271. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
    TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
    GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
    CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
    ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
    AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
    TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
    GCTCCACCCC CTGCTTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276956.1
    CDS547..1407
    Translation

    Target ORF information:

    RefSeq Version NM_001290027.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGAC
    TTCTGGATTG TTGGAGACAA ACTTCACTGC CTCAGCCAGA ACTACTGGCT CTGGGCTTCT
    GAAGTGGCAG CAGGGATTCA GAGCCAAGAT TCCTGGTCTG CTGAACCAAA CCTCCAGGTC
    CCTGGACCAA ATCCCCGGAT ACCTGAACAG GATACACGAA CTCTTGAATG GAACTCGTGG
    ACTCTTTCCT GGACCCTCAC GCAGGACCCT AGGAGCCCCG GACATTTCCT CAGGAACATC
    AGACACAGGC TCCCTGCCAC CCAACCTCCA GCCTGGATAT TCTCCTTCCC CAACCCATCC
    TCCTACTGGA CAGTATACGC TCTTCCCTCT TCCACCCACC TTGCCCACCC CTGTGGTCCA
    GCTCCACCCC CTGCTTCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02046
    Clone ID Related Accession (Same CDS sequence) NM_001177598.2 , NM_001290026.1
    Accession Version NM_001177598.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AB102890.1, AA480029.1 and AA479058.1. On Feb 21, 2014 this sequence version replaced NM_001177598.1. Transcript Variant: This variant (3) represents use of the downstream promoter and comprises six exons. It uses an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. The resulting isoform (3) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB102890.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
    TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
    AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
    CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
    CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
    AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
    CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
    CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
    CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
    CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001171069.1
    CDS280..1239
    Translation

    Target ORF information:

    RefSeq Version NM_001177598.2
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001177598.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
    TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
    AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
    CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
    CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
    AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
    CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
    CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
    CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
    CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu02046
    Clone ID Related Accession (Same CDS sequence) NM_001177598.2 , NM_001290026.1
    Accession Version NM_001290026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 960bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, BC143982.1, AB102890.1, AA480029.1 and AA479058.1. Transcript Variant: This variant (7) represents use of the upstream promoter and comprises seven exons. This variant is longer at the 5' end and has an alternate splice site in the 3' coding region, which results in a frameshift, compared to variant 1. This variant includes two main in-frame AUG sites, but translation initiated from the upstream AUG codon for this variant is not reported in literature. The isoform (3) represented in this RefSeq is derived from the downstream AUG start codon; it is identical to the isoform encoded by variant 3 and lacks an internal segment, as compared to isoform 1. This variant was reported in PMID: 7822271. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB102890.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: experimental evidence (PMID:7822271) regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
    TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
    AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
    CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
    CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
    AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
    CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
    CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
    CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
    CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276955.1
    CDS547..1506
    Translation

    Target ORF information:

    RefSeq Version NM_001290026.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG 
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGACC ACAGCTCACA AGGATCCCAA
    TGCCATCTTC CTGAGCTTCC AACACCTGCT CCGAGGAAAG GTGCGTTTCC TGATGCTTGT
    AGGAGGGTCC ACCCTCTGCG TCAGGCGGGC CCCACCCACC ACAGCTGTCC CCAGCAGAAC
    CTCTCTAGTC CTCACACTGA ACGAGCTCCC AAACAGGACT TCTGGATTGT TGGAGACAAA
    CTTCACTGCC TCAGCCAGAA CTACTGGCTC TGGGCTTCTG AAGTGGCAGC AGGGATTCAG
    AGCCAAGATT CCTGGTCTGC TGAACCAAAC CTCCAGGTCC CTGGACCAAA TCCCCGGATA
    CCTGAACAGG ATACACGAAC TCTTGAATGG AACTCGTGGA CTCTTTCCTG GACCCTCACG
    CAGGACCCTA GGAGCCCCGG ACATTTCCTC AGGAACATCA GACACAGGCT CCCTGCCACC
    CAACCTCCAG CCTGGATATT CTCCTTCCCC AACCCATCCT CCTACTGGAC AGTATACGCT
    CTTCCCTCTT CCACCCACCT TGCCCACCCC TGTGGTCCAG CTCCACCCCC TGCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu11385
    Clone ID Related Accession (Same CDS sequence) NM_001290003.1
    Accession Version NM_001290003.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-22
    Organism Homo sapiens(human)
    Product thrombopoietin isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC078797.20, L33410.1, BC143982.1, BC136338.1 and AA479058.1. Transcript Variant: This variant (5) represents use of the upstream promoter and comprises seven exons. It is longer at the 5' end, compared to variant 1. This variant can initiate translation from two in-frame AUG sites. The isoform (5) represented in this Refseq is derived from the upstream AUG start codon and is longer at the N-terminus, compared to isoform 1. This isoform was described as a uAUG4 product in PMID: 9834204 and had no thrombopoietin bioactivity. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 9834204 ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
    CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
    TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
    AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
    CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
    CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
    GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001276932.1
    CDS127..1608
    Translation

    Target ORF information:

    RefSeq Version NM_001290003.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001290003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
    CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
    TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
    AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
    CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
    CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
    GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGCTTC CTCCACAGGG CAGGACCACA
    GCTCACAAGG ATCCCAATGC CATCTTCCTG AGCTTCCAAC ACCTGCTCCG AGGAAAGGTG
    CGTTTCCTGA TGCTTGTAGG AGGGTCCACC CTCTGCGTCA GGCGGGCCCC ACCCACCACA
    GCTGTCCCCA GCAGAACCTC TCTAGTCCTC ACACTGAACG AGCTCCCAAA CAGGACTTCT
    GGATTGTTGG AGACAAACTT CACTGCCTCA GCCAGAACTA CTGGCTCTGG GCTTCTGAAG
    TGGCAGCAGG GATTCAGAGC CAAGATTCCT GGTCTGCTGA ACCAAACCTC CAGGTCCCTG
    GACCAAATCC CCGGATACCT GAACAGGATA CACGAACTCT TGAATGGAAC TCGTGGACTC
    TTTCCTGGAC CCTCACGCAG GACCCTAGGA GCCCCGGACA TTTCCTCAGG AACATCAGAC
    ACAGGCTCCC TGCCACCCAA CCTCCAGCCT GGATATTCTC CTTCCCCAAC CCATCCTCCT
    ACTGGACAGT ATACGCTCTT CCCTCTTCCA CCCACCTTGC CCACCCCTGT GGTCCAGCTC
    CACCCCCTGC TTCCTGACCC TTCTGCTCCA ACGCCCACCC CTACCAGCCC TCTTCTAAAC
    ACATCCTACA CCCACTCCCA GAATCTGTCT CAGGAAGGGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60994
    Clone ID Related Accession (Same CDS sequence) XM_017007107.1
    Accession Version XM_017007107.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1269bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product thrombopoietin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
    CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
    TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
    AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
    CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
    CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
    GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
    CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGACTT CTGGATTGTT
    GGAGACAAAC TTCACTGCCT CAGCCAGAAC TACTGGCTCT GGGCTTCTGA AGTGGCAGCA
    GGGATTCAGA GCCAAGATTC CTGGTCTGCT GAACCAAACC TCCAGGTCCC TGGACCAAAT
    CCCCGGATAC CTGAACAGGA TACACGAACT CTTGAATGGA ACTCGTGGAC TCTTTCCTGG
    ACCCTCACGC AGGACCCTAG GAGCCCCGGA CATTTCCTCA GGAACATCAG ACACAGGCTC
    CCTGCCACCC AACCTCCAGC CTGGATATTC TCCTTCCCCA ACCCATCCTC CTACTGGACA
    GTATACGCTC TTCCCTCTTC CACCCACCTT GCCCACCCCT GTGGTCCAGC TCCACCCCCT
    GCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016862596.1
    CDS127..1395
    Translation

    Target ORF information:

    RefSeq Version XM_017007107.1
    Organism Homo sapiens(human)
    Definition Homo sapiens thrombopoietin (THPO), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017007107.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGATCCCC CTATCCAAAT CTTCTCCGTG GTGTGTGTGG GTGGAGGAGT GGACCCTGGT 
    CCAGGCAGGG GCTCCAGGGA AGAGAAGGCG TCACTTCCGG GGGCCTTCAC CAGTGTCTGG
    TGGCTCCCTT CTCTGATTGG GCAGAAGTGG CCCAGGCAGG CGTATGACCT GCTGCTGTGG
    AGGGGCTGTG CCCCACCGCC ACATGTCTTC CTACCCATCT GCTCCCCAGA GGGCTGCCTG
    CTGTGCACTT GGGTCCTGGA GCCCTTCTCC ACCCGGATAG ATTCTTCACC CTTGGCCCGC
    CTTTGCCCCA CCCTACTCTG CCCAGAAGTG CAAGAGCCTA AGCCGCCTCC ATGGCCCCAG
    GAAGGATTCA GGGGAGAGGC CCCAAACAGG GAGCCACGCC AGCCAGACAC CCCGGCCAGA
    ATGGAGCTGA CTGAATTGCT CCTCGTGGTC ATGCTTCTCC TAACTGCAAG GCTAACGCTG
    TCCAGCCCGG CTCCTCCTGC TTGTGACCTC CGAGTCCTCA GTAAACTGCT TCGTGACTCC
    CATGTCCTTC ACAGCAGACT GAGCCAGTGC CCAGAGGTTC ACCCTTTGCC TACACCTGTC
    CTGCTGCCTG CTGTGGACTT TAGCTTGGGA GAATGGAAAA CCCAGATGGA GGAGACCAAG
    GCACAGGACA TTCTGGGAGC AGTGACCCTT CTGCTGGAGG GAGTGATGGC AGCACGGGGA
    CAACTGGGAC CCACTTGCCT CTCATCCCTC CTGGGGCAGC TTTCTGGACA GGTCCGTCTC
    CTCCTTGGGG CCCTGCAGAG CCTCCTTGGA ACCCAGGGCA GGACCACAGC TCACAAGGAT
    CCCAATGCCA TCTTCCTGAG CTTCCAACAC CTGCTCCGAG GAAAGGACTT CTGGATTGTT
    GGAGACAAAC TTCACTGCCT CAGCCAGAAC TACTGGCTCT GGGCTTCTGA AGTGGCAGCA
    GGGATTCAGA GCCAAGATTC CTGGTCTGCT GAACCAAACC TCCAGGTCCC TGGACCAAAT
    CCCCGGATAC CTGAACAGGA TACACGAACT CTTGAATGGA ACTCGTGGAC TCTTTCCTGG
    ACCCTCACGC AGGACCCTAG GAGCCCCGGA CATTTCCTCA GGAACATCAG ACACAGGCTC
    CCTGCCACCC AACCTCCAGC CTGGATATTC TCCTTCCCCA ACCCATCCTC CTACTGGACA
    GTATACGCTC TTCCCTCTTC CACCCACCTT GCCCACCCCT GTGGTCCAGC TCCACCCCCT
    GCTTCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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