Eif4g1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Eif4g1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif4g1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu79292 XM_017316920.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $643.30
$919.00
OMu86907 XM_030249027.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OMu72647 NM_001304432.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 22 $811.30
$1159.00
OMu14490 NM_145941.3
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 22 $811.30
$1159.00
OMu14490 XM_006521937.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 22 $811.30
$1159.00
OMu14547 XM_006521940.4
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu51987 XM_006521938.2
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu51990 XM_030249025.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu51991 XM_030249026.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu86905 XM_030249023.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu86906 XM_030249024.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OMu51986 XM_006521935.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51986 XM_006521936.2
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51987 XM_006521939.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51989 XM_006521942.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51990 XM_006521943.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51990 XM_017316919.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51991 XM_006521944.3
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51992 XM_006521945.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51993 XM_006521946.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu72646 XM_011245861.1
Latest version!
Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
View Old Accession Versions >>
OMu14547 XM_006521940.3 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OMu51987 XM_006521938.1 Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu79292
    Clone ID Related Accession (Same CDS sequence) XM_017316920.1
    Accession Version XM_017316920.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
    GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA
    GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC
    ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG
    GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT
    GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA
    GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG
    AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA
    GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA
    GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC
    CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC
    CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC
    TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG
    TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC
    AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG
    CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT
    CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT
    GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC
    AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT
    TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT
    GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC
    CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC
    CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA
    GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA
    GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA
    TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT
    CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT
    CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG
    CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT
    GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT
    ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA
    AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG
    GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT
    CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA
    GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG
    GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT
    CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA
    GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT
    CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG
    TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT
    GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC
    TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG
    GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA
    GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG
    CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG
    CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG
    GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA
    GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG
    GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA
    CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT
    CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA
    TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC
    CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT
    GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG
    CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA
    GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG
    GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT
    GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172409.1
    CDS1143..5054
    Translation

    Target ORF information:

    RefSeq Version XM_017316920.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017316920.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT 
    GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA
    GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC
    ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG
    GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT
    GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA
    GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG
    AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA
    GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA
    GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC
    CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC
    CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC
    TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG
    TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC
    AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG
    CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT
    CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT
    GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC
    AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT
    TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT
    GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC
    CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC
    CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA
    GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA
    GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA
    TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT
    CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT
    CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG
    CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT
    GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT
    ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA
    AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG
    GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT
    CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA
    GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG
    GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT
    CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA
    GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT
    CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG
    TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT
    GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC
    TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG
    GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA
    GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG
    CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG
    CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG
    GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA
    GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG
    GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA
    CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT
    CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA
    TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC
    CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT
    GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG
    CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA
    GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG
    GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT
    GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86907
    Clone ID Related Accession (Same CDS sequence) XM_030249027.1
    Accession Version XM_030249027.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4494bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
    CAGCAGTACC CTGTGCAGCC AGGAGCCCCA GGCTTCTATC CAGGTGCAAG CCCTACCGAG
    TTTGGGACCT ATGCTGGAGC CTATTACCCA GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT
    GTGGCTCCTG CCCCAGTTTT GATGAACCAG CCACCCCAGA TTGCTCCTAA GAGGGAACGG
    AAAACTATCC GAATTCGAGA CCCAAACCAA GGAGGGAAGG ATATCACAGA AGAGATCATG
    TCTGGGGCCC GCACTGCCTC CACACCCACT CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT
    CAACCCAATG GGGAGTCGCC TCAGGTTGCT GTCATTATCC GGCCAGATGA CCGGTCGCAG
    GGAGCAGCCA TTGGGGGGCG GCCAGGACTG CCTGGCCCAG AGCATAGCCC TGGCACAGAA
    TCTCAGCCTT CGTCGCCTTC TCCAACCCCA TCACCACCCC CAATTTTGGA GCCGGGGTCT
    GAGTCTAATC TTGGAGTCCT CTCTATTCCT GGGGACACTA TGACAACAGG GATGATACCA
    ATGTCTGTAG AAGAATCGAC CCCTATCTCT TGTGAAACTG GGGAGCCGTA TTGCCTCTCT
    CCAGAACCCA CTCTTGCCGA ACCCATACTG GAAGTAGAAG TGACACTCAG CAAACCCATT
    CCAGAATCTG AGTTCTCTTC CAGTCCTCTC CAGGTTTCCA CGGCCCTGGT GCCTCACAAG
    GTTGAAACTC ATGAGCCCAA TGGCGTGATC CCATCTGAGG ATCTGGAACC AGAGGTGGAG
    TCAAGCACAG AGCCAGCTCC TCCCCCTCTA TCACCTTGTG CTTCTGAATC GCTCGTGCCC
    ATTGCTCCAA CTGCCCAGCC TGAGGAACTG CTCAACGGAG CCCCCTCACC ACCAGCTGTG
    GATTTAAGCC CAGTCAGTGA GCCAGAGGAA CAGGCCAAGA AGGTTTCATC AGCAGCACTG
    GCCAGCATTC TCTCTCCTGC TCCACCTGTG GCTCCTTCAG ATACTTCTCC TGCTCAGGAG
    GAAGAAATGG AAGAAGATGA CGATGACGAA GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA
    GGAGAGGACG TCCCCCTTGA CAGTACTCCT GTCCCAGCCC AGCTGTCTCA GAATTTAGAG
    GTGGCAGCAG CTACCCAGGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAAGAG
    CTAAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGT GGACCCAGCA
    GTACCAGAGG TAGAGAATCA GCCTCCCACT GGCAGCAACC CAAGCCCAGA GTCTGAGGGC
    AGCATGGTGC CCACACAGCC TGAGGAAACA GAAGAAACCT GGGACTCTAA GGAAGACAAA
    ATTCATAATG CTGAGAACAT CCAGCCTGGG GAGCAGAAGT ATGAGTACAA GTCAGATCAG
    TGGAAGCCTC TAAACCTTGA AGAAAAGAAG CGTTATGACA GAGAATTCCT GCTGGGCTTT
    CAGTTCATCT TTGCCAGCAT GCAGAAGCCA GAAGGATTGC CCCATATCAC TGATGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTTCGG CAACTGGATC CCTCCAGACT ACCTGGCATA
    AACTGTGGCC CAGATTTCAC TCCATCTTTT GCCAATCTTG GCCGACCAGC CCTCAGCAAC
    CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG
    GGACCCAGGC GCTCTCAGCA GGGCCCACGA AAGGAAACTC GCAAGATCAT TTCCTCAGTG
    ATAATGACTG AAGACATAAA ACTAAATAAA GCAGAGAAGG CTTGGAAACC CAGTAGCAAA
    CGGACAGCAG CTGATAAGGA TCGAGGGGAA GAGGATGCTG ATGGAAGCAA GACCCAGGAC
    CTGTTCCGCA GGGTACGCTC CATCCTGAAT AAGCTGACAC CCCAGATGTT TCAGCAGCTG
    ATGAAGCAGG TGACACAGCT GGCCATTGAC ACGGAGGAAC GCCTCAAAGG AGTTATTGAC
    CTCATCTTTG AGAAAGCCAT TTCAGAGCCC AACTTCTCTG TGGCTTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTAAC TGTGAATTTT
    CGAAAACTGT TGTTAAATCG GTGCCAGAAG GAATTTGAGA AAGACAAAGA TGATGATGAA
    GTTTTTGAAA AAAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAAGA ACGGGGACGC
    CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC ATAGCTAGGC GGCGCTCTTT AGGGAATATC
    AAGTTTATTG GAGAGTTATT CAAGTTGAAG ATGTTAACAG AAGCAATAAT GCATGACTGT
    GTGGTCAAGC TACTTAAGAA CCATGATGAG GAGTCCCTGG AATGCCTCTG CCGCCTCCTC
    ACCACTATAG GCAAAGACCT GGACTTTGCA AAAGCCAAGC CTCGAATGGA TCAGTATTTC
    AACCAAATGG AAAAAATCAT TAAAGAAAAG AAGACCTCAT CTCGCATCCG TTTTATGCTG
    CAGGATGTAC TCGATCTGCG GCAGAGTAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT
    AAGACTATTG ATCAAATTCA CAAGGAGGCT GAGATGGAAG AGCACCGAGA ACATATTAAA
    GTACAGCAGC TCATGGCCAA GGGCAGCGAC AAACGTCGGG GTGGCCCTCC AGGCCCGCCT
    ATCAACCGTG GCCTTCCACT TGTAGATGAT GGTGGCTGGA ATACAGTCCC CATTAGCAAA
    GGCAGTCGCC CCATTGACAC CTCACGGCTC ACCAAGATCA CAAAGCCTGG TTCCATTGAT
    TCTAACAACC AGCTTTTTGC ACCTGGAGGA CGACTGAGTT GGGGCAAGGG CAGCAGTGGG
    GGCTCAGGAG CCAAGCCCTC AGACACAGCA TCGGAAGCTA CTCGTCCAGC TACCTTGAAT
    CGCTTTTCTG CTCTTCAACA AACATTACCC GCAGAGAACA CAGATAACAG ACGTGTTGTA
    CAGAGGAGTA GCTTGAGCCG GGAACGAGGT GAGAAAGCTG GGGACCGGGG AGACCGACTA
    GAGCGGAGTG AGCGGGGAGG TGACCGTGGG GACCGACTTG ATCGTGCCAG AACACCTGCC
    ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT
    GAGGGACTCC GCAAGGCAGC TAGCCTCACA GAGGATCGTG GTCGGGATCC TGTGAAGCGG
    GAAGCCACTC TACCTCCAGT GAGCCCTCCA AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG
    AAGAAATCTA AGGCCATCAT TGAGGAATAT CTCCATCTCA ATGACATGAA GGAGGCAGTA
    CAGTGTGTCC AGGAGCTGGC TTCACCCTCC TTGCTCTTCA TCTTCGTACG GCTTGGTATC
    GAGTCCACGC TGGAGCGTAG TACCATTGCT CGTGAGCATA TGGGGCGACT ACTGCACCAA
    CTGCTCTGTG CGGGGCACCT CTCTACTGCC CAGTACTATC AAGGGCTGTA TGAAACACTA
    GAATTGGCTG AGGACATGGA AATTGATATC CCTCATGTAT GGCTTTACCT GGCAGAACTG
    ATTACACCTA TTCTCCAGGA AGATGGGGTG CCCATGGGAG AGCTCTTCAG GGAAATTACG
    AAGCCTCTGA GACCCATGGG CAAAGCTACT TCTTTATTGC TGGAGATCCT GGGTCTCTTA
    TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG
    AGGGAATTTC TAGCAGAGGG CCAAGACGTT GGCTCATTTG TGGCTGAAAA GAAGGTGGAA
    TATACCTTGG GAGAAGAATC TGAAGCTCCT GGCCAGAGGA CACTTGCCTT TGAGGAGCTT
    CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC GGCGGCAGTA ATCAGCGTGT GTTCGACTGG
    ATAGACGCCA ACCTAAATGA GCAGCAGATA GCATCCAATA CATTAGTTCG AGCCCTCATG
    ACAACTGTCT GTTATTCTGC AATTATCTTT GAGACTCCTC TCCGAGTGGA TGTTCAAGTG
    TTGAAAGTGC GAGCAAGACT GCTGCAGAAA TACCTGTGTG ATGAGCAGAA GGAGCTACAA
    GCACTCTATG CTCTCCAGGC CCTTGTAGTG ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG
    ATGTTCTTTG ATGCTCTATA TGATGAGGAC GTGGTGAAGG AAGACGCCTT CTACAGCTGG
    GAGAGCAGCA AGGATCCTGC TGAACAGCAG GGCAAGGGTG TGGCCCTTAA ATCTGTCACA
    GCATTCTTCA ATTGGCTTCG TGAGGCTGAG GACGAGGAGT CTGATCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104887.1
    CDS272..4765
    Translation

    Target ORF information:

    RefSeq Version XM_030249027.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249027.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
    CAGCAGTACC CTGTGCAGCC AGGAGCCCCA GGCTTCTATC CAGGTGCAAG CCCTACCGAG
    TTTGGGACCT ATGCTGGAGC CTATTACCCA GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT
    GTGGCTCCTG CCCCAGTTTT GATGAACCAG CCACCCCAGA TTGCTCCTAA GAGGGAACGG
    AAAACTATCC GAATTCGAGA CCCAAACCAA GGAGGGAAGG ATATCACAGA AGAGATCATG
    TCTGGGGCCC GCACTGCCTC CACACCCACT CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT
    CAACCCAATG GGGAGTCGCC TCAGGTTGCT GTCATTATCC GGCCAGATGA CCGGTCGCAG
    GGAGCAGCCA TTGGGGGGCG GCCAGGACTG CCTGGCCCAG AGCATAGCCC TGGCACAGAA
    TCTCAGCCTT CGTCGCCTTC TCCAACCCCA TCACCACCCC CAATTTTGGA GCCGGGGTCT
    GAGTCTAATC TTGGAGTCCT CTCTATTCCT GGGGACACTA TGACAACAGG GATGATACCA
    ATGTCTGTAG AAGAATCGAC CCCTATCTCT TGTGAAACTG GGGAGCCGTA TTGCCTCTCT
    CCAGAACCCA CTCTTGCCGA ACCCATACTG GAAGTAGAAG TGACACTCAG CAAACCCATT
    CCAGAATCTG AGTTCTCTTC CAGTCCTCTC CAGGTTTCCA CGGCCCTGGT GCCTCACAAG
    GTTGAAACTC ATGAGCCCAA TGGCGTGATC CCATCTGAGG ATCTGGAACC AGAGGTGGAG
    TCAAGCACAG AGCCAGCTCC TCCCCCTCTA TCACCTTGTG CTTCTGAATC GCTCGTGCCC
    ATTGCTCCAA CTGCCCAGCC TGAGGAACTG CTCAACGGAG CCCCCTCACC ACCAGCTGTG
    GATTTAAGCC CAGTCAGTGA GCCAGAGGAA CAGGCCAAGA AGGTTTCATC AGCAGCACTG
    GCCAGCATTC TCTCTCCTGC TCCACCTGTG GCTCCTTCAG ATACTTCTCC TGCTCAGGAG
    GAAGAAATGG AAGAAGATGA CGATGACGAA GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA
    GGAGAGGACG TCCCCCTTGA CAGTACTCCT GTCCCAGCCC AGCTGTCTCA GAATTTAGAG
    GTGGCAGCAG CTACCCAGGT GGCAGTGTCT GTGCCAAAGA GGAGACGGAA AATTAAAGAG
    CTAAATAAGA AGGAGGCTGT AGGAGACCTT CTAGATGCCT TCAAGGAGGT GGACCCAGCA
    GTACCAGAGG TAGAGAATCA GCCTCCCACT GGCAGCAACC CAAGCCCAGA GTCTGAGGGC
    AGCATGGTGC CCACACAGCC TGAGGAAACA GAAGAAACCT GGGACTCTAA GGAAGACAAA
    ATTCATAATG CTGAGAACAT CCAGCCTGGG GAGCAGAAGT ATGAGTACAA GTCAGATCAG
    TGGAAGCCTC TAAACCTTGA AGAAAAGAAG CGTTATGACA GAGAATTCCT GCTGGGCTTT
    CAGTTCATCT TTGCCAGCAT GCAGAAGCCA GAAGGATTGC CCCATATCAC TGATGTGGTG
    CTGGACAAGG CCAATAAAAC ACCACTTCGG CAACTGGATC CCTCCAGACT ACCTGGCATA
    AACTGTGGCC CAGATTTCAC TCCATCTTTT GCCAATCTTG GCCGACCAGC CCTCAGCAAC
    CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG
    GGACCCAGGC GCTCTCAGCA GGGCCCACGA AAGGAAACTC GCAAGATCAT TTCCTCAGTG
    ATAATGACTG AAGACATAAA ACTAAATAAA GCAGAGAAGG CTTGGAAACC CAGTAGCAAA
    CGGACAGCAG CTGATAAGGA TCGAGGGGAA GAGGATGCTG ATGGAAGCAA GACCCAGGAC
    CTGTTCCGCA GGGTACGCTC CATCCTGAAT AAGCTGACAC CCCAGATGTT TCAGCAGCTG
    ATGAAGCAGG TGACACAGCT GGCCATTGAC ACGGAGGAAC GCCTCAAAGG AGTTATTGAC
    CTCATCTTTG AGAAAGCCAT TTCAGAGCCC AACTTCTCTG TGGCTTATGC CAACATGTGC
    CGCTGCCTCA TGGCGCTGAA AGTGCCCACT ACAGAAAAGC CAACAGTAAC TGTGAATTTT
    CGAAAACTGT TGTTAAATCG GTGCCAGAAG GAATTTGAGA AAGACAAAGA TGATGATGAA
    GTTTTTGAAA AAAAGCAAAA AGAGATGGAT GAAGCTGCTA CGGCAGAAGA ACGGGGACGC
    CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC ATAGCTAGGC GGCGCTCTTT AGGGAATATC
    AAGTTTATTG GAGAGTTATT CAAGTTGAAG ATGTTAACAG AAGCAATAAT GCATGACTGT
    GTGGTCAAGC TACTTAAGAA CCATGATGAG GAGTCCCTGG AATGCCTCTG CCGCCTCCTC
    ACCACTATAG GCAAAGACCT GGACTTTGCA AAAGCCAAGC CTCGAATGGA TCAGTATTTC
    AACCAAATGG AAAAAATCAT TAAAGAAAAG AAGACCTCAT CTCGCATCCG TTTTATGCTG
    CAGGATGTAC TCGATCTGCG GCAGAGTAAT TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT
    AAGACTATTG ATCAAATTCA CAAGGAGGCT GAGATGGAAG AGCACCGAGA ACATATTAAA
    GTACAGCAGC TCATGGCCAA GGGCAGCGAC AAACGTCGGG GTGGCCCTCC AGGCCCGCCT
    ATCAACCGTG GCCTTCCACT TGTAGATGAT GGTGGCTGGA ATACAGTCCC CATTAGCAAA
    GGCAGTCGCC CCATTGACAC CTCACGGCTC ACCAAGATCA CAAAGCCTGG TTCCATTGAT
    TCTAACAACC AGCTTTTTGC ACCTGGAGGA CGACTGAGTT GGGGCAAGGG CAGCAGTGGG
    GGCTCAGGAG CCAAGCCCTC AGACACAGCA TCGGAAGCTA CTCGTCCAGC TACCTTGAAT
    CGCTTTTCTG CTCTTCAACA AACATTACCC GCAGAGAACA CAGATAACAG ACGTGTTGTA
    CAGAGGAGTA GCTTGAGCCG GGAACGAGGT GAGAAAGCTG GGGACCGGGG AGACCGACTA
    GAGCGGAGTG AGCGGGGAGG TGACCGTGGG GACCGACTTG ATCGTGCCAG AACACCTGCC
    ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT
    GAGGGACTCC GCAAGGCAGC TAGCCTCACA GAGGATCGTG GTCGGGATCC TGTGAAGCGG
    GAAGCCACTC TACCTCCAGT GAGCCCTCCA AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG
    AAGAAATCTA AGGCCATCAT TGAGGAATAT CTCCATCTCA ATGACATGAA GGAGGCAGTA
    CAGTGTGTCC AGGAGCTGGC TTCACCCTCC TTGCTCTTCA TCTTCGTACG GCTTGGTATC
    GAGTCCACGC TGGAGCGTAG TACCATTGCT CGTGAGCATA TGGGGCGACT ACTGCACCAA
    CTGCTCTGTG CGGGGCACCT CTCTACTGCC CAGTACTATC AAGGGCTGTA TGAAACACTA
    GAATTGGCTG AGGACATGGA AATTGATATC CCTCATGTAT GGCTTTACCT GGCAGAACTG
    ATTACACCTA TTCTCCAGGA AGATGGGGTG CCCATGGGAG AGCTCTTCAG GGAAATTACG
    AAGCCTCTGA GACCCATGGG CAAAGCTACT TCTTTATTGC TGGAGATCCT GGGTCTCTTA
    TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG
    AGGGAATTTC TAGCAGAGGG CCAAGACGTT GGCTCATTTG TGGCTGAAAA GAAGGTGGAA
    TATACCTTGG GAGAAGAATC TGAAGCTCCT GGCCAGAGGA CACTTGCCTT TGAGGAGCTT
    CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC GGCGGCAGTA ATCAGCGTGT GTTCGACTGG
    ATAGACGCCA ACCTAAATGA GCAGCAGATA GCATCCAATA CATTAGTTCG AGCCCTCATG
    ACAACTGTCT GTTATTCTGC AATTATCTTT GAGACTCCTC TCCGAGTGGA TGTTCAAGTG
    TTGAAAGTGC GAGCAAGACT GCTGCAGAAA TACCTGTGTG ATGAGCAGAA GGAGCTACAA
    GCACTCTATG CTCTCCAGGC CCTTGTAGTG ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG
    ATGTTCTTTG ATGCTCTATA TGATGAGGAC GTGGTGAAGG AAGACGCCTT CTACAGCTGG
    GAGAGCAGCA AGGATCCTGC TGAACAGCAG GGCAAGGGTG TGGCCCTTAA ATCTGTCACA
    GCATTCTTCA ATTGGCTTCG TGAGGCTGAG GACGAGGAGT CTGATCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72647
    Clone ID Related Accession (Same CDS sequence) NM_001304432.1
    Accession Version NM_001304432.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4761bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY789918.1, BC066038.1 and BC066103.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. This results in a shorter protein (isoform c), compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066038.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC
    TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA
    GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT
    GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG
    GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG
    ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG
    AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG
    TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG
    CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA
    GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA
    CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC
    AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA
    AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC
    GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT
    CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC
    ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAATGATGGT
    GGCTGGAATA CAGTCCCCAT TAGCAAAGGC AGTCGCCCCA TTGACACCTC ACGGCTCACC
    AAGATCACAA AGCCTGGTTC CATTGATTCT AACAACCAGC TTTTTGCACC TGGAGGACGA
    CTGAGTTGGG GCAAGGGCAG CAGTGGGGGC TCAGGAGCCA AGCCCTCAGA CACAGCATCG
    GAAGCTACTC GTCCAGCTAC CTTGAATCGC TTTTCTGCTC TTCAACAAAC ATTACCCGCA
    GAGAACACAG ATAACAGACG TGTTGTACAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG
    AAAGCTGGGG ACCGGGGAGA CCGACTAGAG CGGAGTGAGC GGGGAGGTGA CCGTGGGGAC
    CGACTTGATC GTGCCAGAAC ACCTGCCACC AAGCGAAGTT TTAGCAAAGA AGTGGAGGAG
    CGAAGTAGAG AGCGGCCATC CCAGCCTGAG GGACTCCGCA AGGCAGCTAG CCTCACAGAG
    GATCGTGGTC GGGATCCTGT GAAGCGGGAA GCCACTCTAC CTCCAGTGAG CCCTCCAAAG
    GCTGCGCTGT CTGTGGATGA GGTGGAGAAG AAATCTAAGG CCATCATTGA GGAATATCTC
    CATCTCAATG ACATGAAGGA GGCAGTACAG TGTGTCCAGG AGCTGGCTTC ACCCTCCTTG
    CTCTTCATCT TCGTACGGCT TGGTATCGAG TCCACGCTGG AGCGTAGTAC CATTGCTCGT
    GAGCATATGG GGCGACTACT GCACCAACTG CTCTGTGCGG GGCACCTCTC TACTGCCCAG
    TACTATCAAG GGCTGTATGA AACACTAGAA TTGGCTGAGG ACATGGAAAT TGATATCCCT
    CATGTATGGC TTTACCTGGC AGAACTGATT ACACCTATTC TCCAGGAAGA TGGGGTGCCC
    ATGGGAGAGC TCTTCAGGGA AATTACGAAG CCTCTGAGAC CCATGGGCAA AGCTACTTCT
    TTATTGCTGG AGATCCTGGG TCTCTTATGC AAGAGCATGG GTCCCAAAAA GGTGGGGATG
    CTGTGGCGAG AGGCTGGGCT GAGCTGGAGG GAATTTCTAG CAGAGGGCCA AGACGTTGGC
    TCATTTGTGG CTGAAAAGAA GGTGGAATAT ACCTTGGGAG AAGAATCTGA AGCTCCTGGC
    CAGAGGACAC TTGCCTTTGA GGAGCTTCGT AGGCAGCTAG AGAAGCTGCT GAAGGACGGC
    GGCAGTAATC AGCGTGTGTT CGACTGGATA GACGCCAACC TAAATGAGCA GCAGATAGCA
    TCCAATACAT TAGTTCGAGC CCTCATGACA ACTGTCTGTT ATTCTGCAAT TATCTTTGAG
    ACTCCTCTCC GAGTGGATGT TCAAGTGTTG AAAGTGCGAG CAAGACTGCT GCAGAAATAC
    CTGTGTGATG AGCAGAAGGA GCTACAAGCA CTCTATGCTC TCCAGGCCCT TGTAGTGACC
    TTAGAACAGC CTGCCAACCT GCTTCGGATG TTCTTTGATG CTCTATATGA TGAGGACGTG
    GTGAAGGAAG ACGCCTTCTA CAGCTGGGAG AGCAGCAAGG ATCCTGCTGA ACAGCAGGGC
    AAGGGTGTGG CCCTTAAATC TGTCACAGCA TTCTTCAATT GGCTTCGTGA GGCTGAGGAC
    GAGGAGTCTG ATCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001291361.1
    CDS185..4945
    Translation

    Target ORF information:

    RefSeq Version NM_001304432.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001304432.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC
    TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA
    GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT
    GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG
    GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG
    ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG
    AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG
    TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG
    CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA
    GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA
    CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC
    AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA
    AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC
    GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT
    CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC
    ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAATGATGGT
    GGCTGGAATA CAGTCCCCAT TAGCAAAGGC AGTCGCCCCA TTGACACCTC ACGGCTCACC
    AAGATCACAA AGCCTGGTTC CATTGATTCT AACAACCAGC TTTTTGCACC TGGAGGACGA
    CTGAGTTGGG GCAAGGGCAG CAGTGGGGGC TCAGGAGCCA AGCCCTCAGA CACAGCATCG
    GAAGCTACTC GTCCAGCTAC CTTGAATCGC TTTTCTGCTC TTCAACAAAC ATTACCCGCA
    GAGAACACAG ATAACAGACG TGTTGTACAG AGGAGTAGCT TGAGCCGGGA ACGAGGTGAG
    AAAGCTGGGG ACCGGGGAGA CCGACTAGAG CGGAGTGAGC GGGGAGGTGA CCGTGGGGAC
    CGACTTGATC GTGCCAGAAC ACCTGCCACC AAGCGAAGTT TTAGCAAAGA AGTGGAGGAG
    CGAAGTAGAG AGCGGCCATC CCAGCCTGAG GGACTCCGCA AGGCAGCTAG CCTCACAGAG
    GATCGTGGTC GGGATCCTGT GAAGCGGGAA GCCACTCTAC CTCCAGTGAG CCCTCCAAAG
    GCTGCGCTGT CTGTGGATGA GGTGGAGAAG AAATCTAAGG CCATCATTGA GGAATATCTC
    CATCTCAATG ACATGAAGGA GGCAGTACAG TGTGTCCAGG AGCTGGCTTC ACCCTCCTTG
    CTCTTCATCT TCGTACGGCT TGGTATCGAG TCCACGCTGG AGCGTAGTAC CATTGCTCGT
    GAGCATATGG GGCGACTACT GCACCAACTG CTCTGTGCGG GGCACCTCTC TACTGCCCAG
    TACTATCAAG GGCTGTATGA AACACTAGAA TTGGCTGAGG ACATGGAAAT TGATATCCCT
    CATGTATGGC TTTACCTGGC AGAACTGATT ACACCTATTC TCCAGGAAGA TGGGGTGCCC
    ATGGGAGAGC TCTTCAGGGA AATTACGAAG CCTCTGAGAC CCATGGGCAA AGCTACTTCT
    TTATTGCTGG AGATCCTGGG TCTCTTATGC AAGAGCATGG GTCCCAAAAA GGTGGGGATG
    CTGTGGCGAG AGGCTGGGCT GAGCTGGAGG GAATTTCTAG CAGAGGGCCA AGACGTTGGC
    TCATTTGTGG CTGAAAAGAA GGTGGAATAT ACCTTGGGAG AAGAATCTGA AGCTCCTGGC
    CAGAGGACAC TTGCCTTTGA GGAGCTTCGT AGGCAGCTAG AGAAGCTGCT GAAGGACGGC
    GGCAGTAATC AGCGTGTGTT CGACTGGATA GACGCCAACC TAAATGAGCA GCAGATAGCA
    TCCAATACAT TAGTTCGAGC CCTCATGACA ACTGTCTGTT ATTCTGCAAT TATCTTTGAG
    ACTCCTCTCC GAGTGGATGT TCAAGTGTTG AAAGTGCGAG CAAGACTGCT GCAGAAATAC
    CTGTGTGATG AGCAGAAGGA GCTACAAGCA CTCTATGCTC TCCAGGCCCT TGTAGTGACC
    TTAGAACAGC CTGCCAACCT GCTTCGGATG TTCTTTGATG CTCTATATGA TGAGGACGTG
    GTGAAGGAAG ACGCCTTCTA CAGCTGGGAG AGCAGCAAGG ATCCTGCTGA ACAGCAGGGC
    AAGGGTGTGG CCCTTAAATC TGTCACAGCA TTCTTCAATT GGCTTCGTGA GGCTGAGGAC
    GAGGAGTCTG ATCACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14490
    Clone ID Related Accession (Same CDS sequence) XM_006521937.1 , NM_145941.3
    Accession Version NM_145941.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY789918.1 and BC066103.1. On Jan 27, 2015 this sequence version replaced NM_145941.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC066103.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849379, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT
    TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC
    AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG
    ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT
    CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA
    GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC
    AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT
    GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT
    GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA
    CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA
    GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG
    CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC
    CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT
    CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT
    TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT
    CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA
    CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA
    GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC
    ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT
    TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC
    AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT
    ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA
    CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA
    ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA
    TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT
    GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT
    GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG
    GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG
    CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA
    CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT
    GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG
    GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG
    GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG
    GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG
    CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG
    AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT
    GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG
    TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA
    GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT
    GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG
    GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC
    CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC
    TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_666053.2
    CDS185..4987
    Translation

    Target ORF information:

    RefSeq Version NM_145941.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_145941.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT
    TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC
    AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG
    ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT
    CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA
    GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC
    AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT
    GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT
    GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA
    CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA
    GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG
    CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC
    CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT
    CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT
    TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT
    CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA
    CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA
    GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC
    ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT
    TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC
    AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT
    ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA
    CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA
    ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA
    TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT
    GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT
    GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG
    GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG
    CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA
    CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT
    GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG
    GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG
    GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG
    GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG
    CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG
    AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT
    GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG
    TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA
    GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT
    GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG
    GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC
    CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC
    TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14490
    Clone ID Related Accession (Same CDS sequence) XM_006521937.1 , NM_145941.3
    Accession Version XM_006521937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4803bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT
    TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC
    AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG
    ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT
    CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA
    GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC
    AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT
    GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT
    GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA
    CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA
    GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG
    CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC
    CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT
    CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT
    TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT
    CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA
    CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA
    GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC
    ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT
    TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC
    AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT
    ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA
    CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA
    ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA
    TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT
    GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT
    GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG
    GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG
    CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA
    CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT
    GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG
    GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG
    GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG
    GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG
    CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG
    AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT
    GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG
    TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA
    GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT
    GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG
    GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC
    CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC
    TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522000.1
    CDS249..5051
    Translation

    Target ORF information:

    RefSeq Version XM_006521937.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    GCTGGCCTGG GACCCAGGCG CTCTCAGCAG GGCCCACGAA AGGAAACTCG CAAGATCATT
    TCCTCAGTGA TAATGACTGA AGACATAAAA CTAAATAAAG CAGAGAAGGC TTGGAAACCC
    AGTAGCAAAC GGACAGCAGC TGATAAGGAT CGAGGGGAAG AGGATGCTGA TGGAAGCAAG
    ACCCAGGACC TGTTCCGCAG GGTACGCTCC ATCCTGAATA AGCTGACACC CCAGATGTTT
    CAGCAGCTGA TGAAGCAGGT GACACAGCTG GCCATTGACA CGGAGGAACG CCTCAAAGGA
    GTTATTGACC TCATCTTTGA GAAAGCCATT TCAGAGCCCA ACTTCTCTGT GGCTTATGCC
    AACATGTGCC GCTGCCTCAT GGCGCTGAAA GTGCCCACTA CAGAAAAGCC AACAGTAACT
    GTGAATTTTC GAAAACTGTT GTTAAATCGG TGCCAGAAGG AATTTGAGAA AGACAAAGAT
    GATGATGAAG TTTTTGAAAA AAAGCAAAAA GAGATGGATG AAGCTGCTAC GGCAGAAGAA
    CGGGGACGCC TGAAAGAAGA GCTAGAAGAA GCCCGGGACA TAGCTAGGCG GCGCTCTTTA
    GGGAATATCA AGTTTATTGG AGAGTTATTC AAGTTGAAGA TGTTAACAGA AGCAATAATG
    CATGACTGTG TGGTCAAGCT ACTTAAGAAC CATGATGAGG AGTCCCTGGA ATGCCTCTGC
    CGCCTCCTCA CCACTATAGG CAAAGACCTG GACTTTGCAA AAGCCAAGCC TCGAATGGAT
    CAGTATTTCA ACCAAATGGA AAAAATCATT AAAGAAAAGA AGACCTCATC TCGCATCCGT
    TTTATGCTGC AGGATGTACT CGATCTGCGG CAGAGTAATT GGGTGCCACG CCGAGGGGAT
    CAGGGTCCTA AGACTATTGA TCAAATTCAC AAGGAGGCTG AGATGGAAGA GCACCGAGAA
    CATATTAAAG TACAGCAGCT CATGGCCAAG GGCAGCGACA AACGTCGGGG TGGCCCTCCA
    GGCCCGCCTA TCAACCGTGG CCTTCCACTT GTAGATGATG GTGGCTGGAA TACAGTCCCC
    ATTAGCAAAG GCAGTCGCCC CATTGACACC TCACGGCTCA CCAAGATCAC AAAGCCTGGT
    TCCATTGATT CTAACAACCA GCTTTTTGCA CCTGGAGGAC GACTGAGTTG GGGCAAGGGC
    AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA GACACAGCAT CGGAAGCTAC TCGTCCAGCT
    ACCTTGAATC GCTTTTCTGC TCTTCAACAA ACATTACCCG CAGAGAACAC AGATAACAGA
    CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG GAACGAGGTG AGAAAGCTGG GGACCGGGGA
    GACCGACTAG AGCGGAGTGA GCGGGGAGGT GACCGTGGGG ACCGACTTGA TCGTGCCAGA
    ACACCTGCCA CCAAGCGAAG TTTTAGCAAA GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA
    TCCCAGCCTG AGGGACTCCG CAAGGCAGCT AGCCTCACAG AGGATCGTGG TCGGGATCCT
    GTGAAGCGGG AAGCCACTCT ACCTCCAGTG AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT
    GAGGTGGAGA AGAAATCTAA GGCCATCATT GAGGAATATC TCCATCTCAA TGACATGAAG
    GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT TCACCCTCCT TGCTCTTCAT CTTCGTACGG
    CTTGGTATCG AGTCCACGCT GGAGCGTAGT ACCATTGCTC GTGAGCATAT GGGGCGACTA
    CTGCACCAAC TGCTCTGTGC GGGGCACCTC TCTACTGCCC AGTACTATCA AGGGCTGTAT
    GAAACACTAG AATTGGCTGA GGACATGGAA ATTGATATCC CTCATGTATG GCTTTACCTG
    GCAGAACTGA TTACACCTAT TCTCCAGGAA GATGGGGTGC CCATGGGAGA GCTCTTCAGG
    GAAATTACGA AGCCTCTGAG ACCCATGGGC AAAGCTACTT CTTTATTGCT GGAGATCCTG
    GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG
    CTGAGCTGGA GGGAATTTCT AGCAGAGGGC CAAGACGTTG GCTCATTTGT GGCTGAAAAG
    AAGGTGGAAT ATACCTTGGG AGAAGAATCT GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT
    GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG CTGAAGGACG GCGGCAGTAA TCAGCGTGTG
    TTCGACTGGA TAGACGCCAA CCTAAATGAG CAGCAGATAG CATCCAATAC ATTAGTTCGA
    GCCCTCATGA CAACTGTCTG TTATTCTGCA ATTATCTTTG AGACTCCTCT CCGAGTGGAT
    GTTCAAGTGT TGAAAGTGCG AGCAAGACTG CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG
    GAGCTACAAG CACTCTATGC TCTCCAGGCC CTTGTAGTGA CCTTAGAACA GCCTGCCAAC
    CTGCTTCGGA TGTTCTTTGA TGCTCTATAT GATGAGGACG TGGTGAAGGA AGACGCCTTC
    TACAGCTGGG AGAGCAGCAA GGATCCTGCT GAACAGCAGG GCAAGGGTGT GGCCCTTAAA
    TCTGTCACAG CATTCTTCAA TTGGCTTCGT GAGGCTGAGG ACGAGGAGTC TGATCACAAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14547
    Clone ID Related Accession (Same CDS sequence) XM_006521940.4 , XM_006521940.3
    Accession Version XM_006521940.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521940.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC
    TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA
    GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT
    GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG
    GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG
    ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG
    AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG
    TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG
    CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA
    GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA
    CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC
    AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA
    AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC
    GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT
    CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC
    ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC
    CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC
    ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG
    CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC
    AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT
    CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC
    TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG
    CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT
    TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC
    AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA
    CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG
    GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG
    GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG
    GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG
    GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG
    GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT
    CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA
    CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG
    GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA
    GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA
    GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA
    GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC
    CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT
    TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA
    GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT
    GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG
    GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT
    TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522003.1
    CDS230..5011
    Translation

    Target ORF information:

    RefSeq Version XM_006521940.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521940.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC
    TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA
    GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT
    GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG
    GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG
    ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG
    AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG
    TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG
    CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA
    GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA
    CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC
    AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA
    AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC
    GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT
    CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC
    ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC
    CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC
    ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG
    CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC
    AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT
    CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC
    TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG
    CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT
    TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC
    AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA
    CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG
    GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG
    GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG
    GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG
    GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG
    GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT
    CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA
    CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG
    GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA
    GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA
    GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA
    GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC
    CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT
    TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA
    GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT
    GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG
    GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT
    TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51987
    Clone ID Related Accession (Same CDS sequence) XM_006521938.1 , XM_006521939.1 , XM_006521938.2
    Accession Version XM_006521938.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006521938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522001.1
    CDS242..5026
    Translation

    Target ORF information:

    RefSeq Version XM_006521938.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521938.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51990
    Clone ID Related Accession (Same CDS sequence) XM_030249025.1 , XM_017316919.1 , XM_006521943.1
    Accession Version XM_030249025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104885.1
    CDS218..4903
    Translation

    Target ORF information:

    RefSeq Version XM_030249025.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51991
    Clone ID Related Accession (Same CDS sequence) XM_006521944.3 , XM_030249026.1
    Accession Version XM_030249026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104886.1
    CDS218..4882
    Translation

    Target ORF information:

    RefSeq Version XM_030249026.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86905
    Clone ID Related Accession (Same CDS sequence) XM_030249023.1
    Accession Version XM_030249023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4773bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG
    CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA
    GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT
    TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA
    GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC
    TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG
    CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG
    CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT
    TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC
    CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG
    ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC
    GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT
    TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC
    AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT
    CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG
    CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT
    GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT
    GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT
    GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT
    GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG
    GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT
    GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT
    CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG
    CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC
    ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT
    GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC
    ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA
    ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC
    ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT
    GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG GCTGGCCTGG GACCCAGGCG CTCTCAGCAG
    GGCCCACGAA AGGAAACTCG CAAGATCATT TCCTCAGTGA TAATGACTGA AGACATAAAA
    CTAAATAAAG CAGAGAAGGC TTGGAAACCC AGTAGCAAAC GGACAGCAGC TGATAAGGAT
    CGAGGGGAAG AGGATGCTGA TGGAAGCAAG ACCCAGGACC TGTTCCGCAG GGTACGCTCC
    ATCCTGAATA AGCTGACACC CCAGATGTTT CAGCAGCTGA TGAAGCAGGT GACACAGCTG
    GCCATTGACA CGGAGGAACG CCTCAAAGGA GTTATTGACC TCATCTTTGA GAAAGCCATT
    TCAGAGCCCA ACTTCTCTGT GGCTTATGCC AACATGTGCC GCTGCCTCAT GGCGCTGAAA
    GTGCCCACTA CAGAAAAGCC AACAGTAACT GTGAATTTTC GAAAACTGTT GTTAAATCGG
    TGCCAGAAGG AATTTGAGAA AGACAAAGAT GATGATGAAG TTTTTGAAAA AAAGCAAAAA
    GAGATGGATG AAGCTGCTAC GGCAGAAGAA CGGGGACGCC TGAAAGAAGA GCTAGAAGAA
    GCCCGGGACA TAGCTAGGCG GCGCTCTTTA GGGAATATCA AGTTTATTGG AGAGTTATTC
    AAGTTGAAGA TGTTAACAGA AGCAATAATG CATGACTGTG TGGTCAAGCT ACTTAAGAAC
    CATGATGAGG AGTCCCTGGA ATGCCTCTGC CGCCTCCTCA CCACTATAGG CAAAGACCTG
    GACTTTGCAA AAGCCAAGCC TCGAATGGAT CAGTATTTCA ACCAAATGGA AAAAATCATT
    AAAGAAAAGA AGACCTCATC TCGCATCCGT TTTATGCTGC AGGATGTACT CGATCTGCGG
    CAGAGTAATT GGGTGCCACG CCGAGGGGAT CAGGGTCCTA AGACTATTGA TCAAATTCAC
    AAGGAGGCTG AGATGGAAGA GCACCGAGAA CATATTAAAG TACAGCAGCT CATGGCCAAG
    GGCAGCGACA AACGTCGGGG TGGCCCTCCA GGCCCGCCTA TCAACCGTGG CCTTCCACTT
    GTAGATGATG GTGGCTGGAA TACAGTCCCC ATTAGCAAAG GCAGTCGCCC CATTGACACC
    TCACGGCTCA CCAAGATCAC AAAGCCTGGT TCCATTGATT CTAACAACCA GCTTTTTGCA
    CCTGGAGGAC GACTGAGTTG GGGCAAGGGC AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA
    GACACAGCAT CGGAAGCTAC TCGTCCAGCT ACCTTGAATC GCTTTTCTGC TCTTCAACAA
    ACATTACCCG CAGAGAACAC AGATAACAGA CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG
    GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGACTAG AGCGGAGTGA GCGGGGAGGT
    GACCGTGGGG ACCGACTTGA TCGTGCCAGA ACACCTGCCA CCAAGCGAAG TTTTAGCAAA
    GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA TCCCAGCCTG AGGGACTCCG CAAGGCAGCT
    AGCCTCACAG AGGATCGTGG TCGGGATCCT GTGAAGCGGG AAGCCACTCT ACCTCCAGTG
    AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT GAGGTGGAGA AGAAATCTAA GGCCATCATT
    GAGGAATATC TCCATCTCAA TGACATGAAG GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT
    TCACCCTCCT TGCTCTTCAT CTTCGTACGG CTTGGTATCG AGTCCACGCT GGAGCGTAGT
    ACCATTGCTC GTGAGCATAT GGGGCGACTA CTGCACCAAC TGCTCTGTGC GGGGCACCTC
    TCTACTGCCC AGTACTATCA AGGGCTGTAT GAAACACTAG AATTGGCTGA GGACATGGAA
    ATTGATATCC CTCATGTATG GCTTTACCTG GCAGAACTGA TTACACCTAT TCTCCAGGAA
    GATGGGGTGC CCATGGGAGA GCTCTTCAGG GAAATTACGA AGCCTCTGAG ACCCATGGGC
    AAAGCTACTT CTTTATTGCT GGAGATCCTG GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA
    AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG CTGAGCTGGA GGGAATTTCT AGCAGAGGGC
    CAAGACGTTG GCTCATTTGT GGCTGAAAAG AAGGTGGAAT ATACCTTGGG AGAAGAATCT
    GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG
    CTGAAGGACG GCGGCAGTAA TCAGCGTGTG TTCGACTGGA TAGACGCCAA CCTAAATGAG
    CAGCAGATAG CATCCAATAC ATTAGTTCGA GCCCTCATGA CAACTGTCTG TTATTCTGCA
    ATTATCTTTG AGACTCCTCT CCGAGTGGAT GTTCAAGTGT TGAAAGTGCG AGCAAGACTG
    CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG GAGCTACAAG CACTCTATGC TCTCCAGGCC
    CTTGTAGTGA CCTTAGAACA GCCTGCCAAC CTGCTTCGGA TGTTCTTTGA TGCTCTATAT
    GATGAGGACG TGGTGAAGGA AGACGCCTTC TACAGCTGGG AGAGCAGCAA GGATCCTGCT
    GAACAGCAGG GCAAGGGTGT GGCCCTTAAA TCTGTCACAG CATTCTTCAA TTGGCTTCGT
    GAGGCTGAGG ACGAGGAGTC TGATCACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104883.1
    CDS249..5021
    Translation

    Target ORF information:

    RefSeq Version XM_030249023.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG
    CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA
    GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT
    TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA
    GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC
    TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG
    CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG
    CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT
    TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC
    CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG
    ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC
    GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT
    TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC
    AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT
    CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG
    CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT
    GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT
    GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT
    GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT
    GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG
    GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT
    GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT
    CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG
    CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC
    ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT
    GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC
    ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA
    ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC
    ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT
    GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG GCTGGCCTGG GACCCAGGCG CTCTCAGCAG
    GGCCCACGAA AGGAAACTCG CAAGATCATT TCCTCAGTGA TAATGACTGA AGACATAAAA
    CTAAATAAAG CAGAGAAGGC TTGGAAACCC AGTAGCAAAC GGACAGCAGC TGATAAGGAT
    CGAGGGGAAG AGGATGCTGA TGGAAGCAAG ACCCAGGACC TGTTCCGCAG GGTACGCTCC
    ATCCTGAATA AGCTGACACC CCAGATGTTT CAGCAGCTGA TGAAGCAGGT GACACAGCTG
    GCCATTGACA CGGAGGAACG CCTCAAAGGA GTTATTGACC TCATCTTTGA GAAAGCCATT
    TCAGAGCCCA ACTTCTCTGT GGCTTATGCC AACATGTGCC GCTGCCTCAT GGCGCTGAAA
    GTGCCCACTA CAGAAAAGCC AACAGTAACT GTGAATTTTC GAAAACTGTT GTTAAATCGG
    TGCCAGAAGG AATTTGAGAA AGACAAAGAT GATGATGAAG TTTTTGAAAA AAAGCAAAAA
    GAGATGGATG AAGCTGCTAC GGCAGAAGAA CGGGGACGCC TGAAAGAAGA GCTAGAAGAA
    GCCCGGGACA TAGCTAGGCG GCGCTCTTTA GGGAATATCA AGTTTATTGG AGAGTTATTC
    AAGTTGAAGA TGTTAACAGA AGCAATAATG CATGACTGTG TGGTCAAGCT ACTTAAGAAC
    CATGATGAGG AGTCCCTGGA ATGCCTCTGC CGCCTCCTCA CCACTATAGG CAAAGACCTG
    GACTTTGCAA AAGCCAAGCC TCGAATGGAT CAGTATTTCA ACCAAATGGA AAAAATCATT
    AAAGAAAAGA AGACCTCATC TCGCATCCGT TTTATGCTGC AGGATGTACT CGATCTGCGG
    CAGAGTAATT GGGTGCCACG CCGAGGGGAT CAGGGTCCTA AGACTATTGA TCAAATTCAC
    AAGGAGGCTG AGATGGAAGA GCACCGAGAA CATATTAAAG TACAGCAGCT CATGGCCAAG
    GGCAGCGACA AACGTCGGGG TGGCCCTCCA GGCCCGCCTA TCAACCGTGG CCTTCCACTT
    GTAGATGATG GTGGCTGGAA TACAGTCCCC ATTAGCAAAG GCAGTCGCCC CATTGACACC
    TCACGGCTCA CCAAGATCAC AAAGCCTGGT TCCATTGATT CTAACAACCA GCTTTTTGCA
    CCTGGAGGAC GACTGAGTTG GGGCAAGGGC AGCAGTGGGG GCTCAGGAGC CAAGCCCTCA
    GACACAGCAT CGGAAGCTAC TCGTCCAGCT ACCTTGAATC GCTTTTCTGC TCTTCAACAA
    ACATTACCCG CAGAGAACAC AGATAACAGA CGTGTTGTAC AGAGGAGTAG CTTGAGCCGG
    GAACGAGGTG AGAAAGCTGG GGACCGGGGA GACCGACTAG AGCGGAGTGA GCGGGGAGGT
    GACCGTGGGG ACCGACTTGA TCGTGCCAGA ACACCTGCCA CCAAGCGAAG TTTTAGCAAA
    GAAGTGGAGG AGCGAAGTAG AGAGCGGCCA TCCCAGCCTG AGGGACTCCG CAAGGCAGCT
    AGCCTCACAG AGGATCGTGG TCGGGATCCT GTGAAGCGGG AAGCCACTCT ACCTCCAGTG
    AGCCCTCCAA AGGCTGCGCT GTCTGTGGAT GAGGTGGAGA AGAAATCTAA GGCCATCATT
    GAGGAATATC TCCATCTCAA TGACATGAAG GAGGCAGTAC AGTGTGTCCA GGAGCTGGCT
    TCACCCTCCT TGCTCTTCAT CTTCGTACGG CTTGGTATCG AGTCCACGCT GGAGCGTAGT
    ACCATTGCTC GTGAGCATAT GGGGCGACTA CTGCACCAAC TGCTCTGTGC GGGGCACCTC
    TCTACTGCCC AGTACTATCA AGGGCTGTAT GAAACACTAG AATTGGCTGA GGACATGGAA
    ATTGATATCC CTCATGTATG GCTTTACCTG GCAGAACTGA TTACACCTAT TCTCCAGGAA
    GATGGGGTGC CCATGGGAGA GCTCTTCAGG GAAATTACGA AGCCTCTGAG ACCCATGGGC
    AAAGCTACTT CTTTATTGCT GGAGATCCTG GGTCTCTTAT GCAAGAGCAT GGGTCCCAAA
    AAGGTGGGGA TGCTGTGGCG AGAGGCTGGG CTGAGCTGGA GGGAATTTCT AGCAGAGGGC
    CAAGACGTTG GCTCATTTGT GGCTGAAAAG AAGGTGGAAT ATACCTTGGG AGAAGAATCT
    GAAGCTCCTG GCCAGAGGAC ACTTGCCTTT GAGGAGCTTC GTAGGCAGCT AGAGAAGCTG
    CTGAAGGACG GCGGCAGTAA TCAGCGTGTG TTCGACTGGA TAGACGCCAA CCTAAATGAG
    CAGCAGATAG CATCCAATAC ATTAGTTCGA GCCCTCATGA CAACTGTCTG TTATTCTGCA
    ATTATCTTTG AGACTCCTCT CCGAGTGGAT GTTCAAGTGT TGAAAGTGCG AGCAAGACTG
    CTGCAGAAAT ACCTGTGTGA TGAGCAGAAG GAGCTACAAG CACTCTATGC TCTCCAGGCC
    CTTGTAGTGA CCTTAGAACA GCCTGCCAAC CTGCTTCGGA TGTTCTTTGA TGCTCTATAT
    GATGAGGACG TGGTGAAGGA AGACGCCTTC TACAGCTGGG AGAGCAGCAA GGATCCTGCT
    GAACAGCAGG GCAAGGGTGT GGCCCTTAAA TCTGTCACAG CATTCTTCAA TTGGCTTCGT
    GAGGCTGAGG ACGAGGAGTC TGATCACAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu86906
    Clone ID Related Accession (Same CDS sequence) XM_030249024.1
    Accession Version XM_030249024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT
    CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT
    GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG
    ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG
    GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG
    ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC
    CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA
    GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC
    CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT
    ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT
    GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA
    GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC
    ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG
    GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT
    GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA
    GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG
    AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA
    GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA
    GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC
    CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC
    CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC
    TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG
    TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC
    AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG
    CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT
    CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT
    GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC
    AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT
    TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT
    GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC
    CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC
    CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA
    GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA
    GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA
    TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT
    CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT
    CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG
    CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT
    GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT
    ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA
    AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG
    GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT
    CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA
    GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG
    GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT
    CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA
    GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT
    CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG
    TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT
    GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC
    TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG
    GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA
    GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG
    CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG
    CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG
    GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA
    GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG
    GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA
    CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT
    CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA
    TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC
    CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT
    GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG
    CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA
    GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG
    GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT
    GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030104884.1
    CDS249..5000
    Translation

    Target ORF information:

    RefSeq Version XM_030249024.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030249024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT
    CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT
    GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG
    ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG
    GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG
    ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC
    CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA
    GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC
    CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT
    ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT
    GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA
    GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC
    ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG
    GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT
    GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA
    GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG
    AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA
    GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA
    GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC
    CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC
    CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC
    TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG
    TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC
    AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG
    CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT
    CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT
    GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC TCTCAGCAGG GCCCACGAAA GGAAACTCGC
    AAGATCATTT CCTCAGTGAT AATGACTGAA GACATAAAAC TAAATAAAGC AGAGAAGGCT
    TGGAAACCCA GTAGCAAACG GACAGCAGCT GATAAGGATC GAGGGGAAGA GGATGCTGAT
    GGAAGCAAGA CCCAGGACCT GTTCCGCAGG GTACGCTCCA TCCTGAATAA GCTGACACCC
    CAGATGTTTC AGCAGCTGAT GAAGCAGGTG ACACAGCTGG CCATTGACAC GGAGGAACGC
    CTCAAAGGAG TTATTGACCT CATCTTTGAG AAAGCCATTT CAGAGCCCAA CTTCTCTGTG
    GCTTATGCCA ACATGTGCCG CTGCCTCATG GCGCTGAAAG TGCCCACTAC AGAAAAGCCA
    ACAGTAACTG TGAATTTTCG AAAACTGTTG TTAAATCGGT GCCAGAAGGA ATTTGAGAAA
    GACAAAGATG ATGATGAAGT TTTTGAAAAA AAGCAAAAAG AGATGGATGA AGCTGCTACG
    GCAGAAGAAC GGGGACGCCT GAAAGAAGAG CTAGAAGAAG CCCGGGACAT AGCTAGGCGG
    CGCTCTTTAG GGAATATCAA GTTTATTGGA GAGTTATTCA AGTTGAAGAT GTTAACAGAA
    GCAATAATGC ATGACTGTGT GGTCAAGCTA CTTAAGAACC ATGATGAGGA GTCCCTGGAA
    TGCCTCTGCC GCCTCCTCAC CACTATAGGC AAAGACCTGG ACTTTGCAAA AGCCAAGCCT
    CGAATGGATC AGTATTTCAA CCAAATGGAA AAAATCATTA AAGAAAAGAA GACCTCATCT
    CGCATCCGTT TTATGCTGCA GGATGTACTC GATCTGCGGC AGAGTAATTG GGTGCCACGC
    CGAGGGGATC AGGGTCCTAA GACTATTGAT CAAATTCACA AGGAGGCTGA GATGGAAGAG
    CACCGAGAAC ATATTAAAGT ACAGCAGCTC ATGGCCAAGG GCAGCGACAA ACGTCGGGGT
    GGCCCTCCAG GCCCGCCTAT CAACCGTGGC CTTCCACTTG TAGATGATGG TGGCTGGAAT
    ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC ATTGACACCT CACGGCTCAC CAAGATCACA
    AAGCCTGGTT CCATTGATTC TAACAACCAG CTTTTTGCAC CTGGAGGACG ACTGAGTTGG
    GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC AAGCCCTCAG ACACAGCATC GGAAGCTACT
    CGTCCAGCTA CCTTGAATCG CTTTTCTGCT CTTCAACAAA CATTACCCGC AGAGAACACA
    GATAACAGAC GTGTTGTACA GAGGAGTAGC TTGAGCCGGG AACGAGGTGA GAAAGCTGGG
    GACCGGGGAG ACCGACTAGA GCGGAGTGAG CGGGGAGGTG ACCGTGGGGA CCGACTTGAT
    CGTGCCAGAA CACCTGCCAC CAAGCGAAGT TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA
    GAGCGGCCAT CCCAGCCTGA GGGACTCCGC AAGGCAGCTA GCCTCACAGA GGATCGTGGT
    CGGGATCCTG TGAAGCGGGA AGCCACTCTA CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG
    TCTGTGGATG AGGTGGAGAA GAAATCTAAG GCCATCATTG AGGAATATCT CCATCTCAAT
    GACATGAAGG AGGCAGTACA GTGTGTCCAG GAGCTGGCTT CACCCTCCTT GCTCTTCATC
    TTCGTACGGC TTGGTATCGA GTCCACGCTG GAGCGTAGTA CCATTGCTCG TGAGCATATG
    GGGCGACTAC TGCACCAACT GCTCTGTGCG GGGCACCTCT CTACTGCCCA GTACTATCAA
    GGGCTGTATG AAACACTAGA ATTGGCTGAG GACATGGAAA TTGATATCCC TCATGTATGG
    CTTTACCTGG CAGAACTGAT TACACCTATT CTCCAGGAAG ATGGGGTGCC CATGGGAGAG
    CTCTTCAGGG AAATTACGAA GCCTCTGAGA CCCATGGGCA AAGCTACTTC TTTATTGCTG
    GAGATCCTGG GTCTCTTATG CAAGAGCATG GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA
    GAGGCTGGGC TGAGCTGGAG GGAATTTCTA GCAGAGGGCC AAGACGTTGG CTCATTTGTG
    GCTGAAAAGA AGGTGGAATA TACCTTGGGA GAAGAATCTG AAGCTCCTGG CCAGAGGACA
    CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA GAGAAGCTGC TGAAGGACGG CGGCAGTAAT
    CAGCGTGTGT TCGACTGGAT AGACGCCAAC CTAAATGAGC AGCAGATAGC ATCCAATACA
    TTAGTTCGAG CCCTCATGAC AACTGTCTGT TATTCTGCAA TTATCTTTGA GACTCCTCTC
    CGAGTGGATG TTCAAGTGTT GAAAGTGCGA GCAAGACTGC TGCAGAAATA CCTGTGTGAT
    GAGCAGAAGG AGCTACAAGC ACTCTATGCT CTCCAGGCCC TTGTAGTGAC CTTAGAACAG
    CCTGCCAACC TGCTTCGGAT GTTCTTTGAT GCTCTATATG ATGAGGACGT GGTGAAGGAA
    GACGCCTTCT ACAGCTGGGA GAGCAGCAAG GATCCTGCTG AACAGCAGGG CAAGGGTGTG
    GCCCTTAAAT CTGTCACAGC ATTCTTCAAT TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT
    GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu14547
    Clone ID Related Accession (Same CDS sequence) XM_006521940.4 , XM_006521940.3
    Accession Version XM_006521940.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521940.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC
    TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA
    GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT
    GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG
    GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG
    ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG
    AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG
    TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG
    CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA
    GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA
    CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC
    AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA
    AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC
    GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT
    CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC
    ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC
    CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC
    ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG
    CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC
    AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT
    CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC
    TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG
    CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT
    TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC
    AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA
    CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG
    GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG
    GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG
    GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG
    GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG
    GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT
    CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA
    CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG
    GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA
    GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA
    GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA
    GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC
    CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT
    TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA
    GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT
    GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG
    GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT
    TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522003.1
    CDS220..5001
    Translation

    Target ORF information:

    RefSeq Version XM_006521940.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521940.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGG CTGGCCTGGG ACCCAGGCGC
    TCTCAGCAGG GCCCACGAAA GGAAACTCGC AAGATCATTT CCTCAGTGAT AATGACTGAA
    GACATAAAAC TAAATAAAGC AGAGAAGGCT TGGAAACCCA GTAGCAAACG GACAGCAGCT
    GATAAGGATC GAGGGGAAGA GGATGCTGAT GGAAGCAAGA CCCAGGACCT GTTCCGCAGG
    GTACGCTCCA TCCTGAATAA GCTGACACCC CAGATGTTTC AGCAGCTGAT GAAGCAGGTG
    ACACAGCTGG CCATTGACAC GGAGGAACGC CTCAAAGGAG TTATTGACCT CATCTTTGAG
    AAAGCCATTT CAGAGCCCAA CTTCTCTGTG GCTTATGCCA ACATGTGCCG CTGCCTCATG
    GCGCTGAAAG TGCCCACTAC AGAAAAGCCA ACAGTAACTG TGAATTTTCG AAAACTGTTG
    TTAAATCGGT GCCAGAAGGA ATTTGAGAAA GACAAAGATG ATGATGAAGT TTTTGAAAAA
    AAGCAAAAAG AGATGGATGA AGCTGCTACG GCAGAAGAAC GGGGACGCCT GAAAGAAGAG
    CTAGAAGAAG CCCGGGACAT AGCTAGGCGG CGCTCTTTAG GGAATATCAA GTTTATTGGA
    GAGTTATTCA AGTTGAAGAT GTTAACAGAA GCAATAATGC ATGACTGTGT GGTCAAGCTA
    CTTAAGAACC ATGATGAGGA GTCCCTGGAA TGCCTCTGCC GCCTCCTCAC CACTATAGGC
    AAAGACCTGG ACTTTGCAAA AGCCAAGCCT CGAATGGATC AGTATTTCAA CCAAATGGAA
    AAAATCATTA AAGAAAAGAA GACCTCATCT CGCATCCGTT TTATGCTGCA GGATGTACTC
    GATCTGCGGC AGAGTAATTG GGTGCCACGC CGAGGGGATC AGGGTCCTAA GACTATTGAT
    CAAATTCACA AGGAGGCTGA GATGGAAGAG CACCGAGAAC ATATTAAAGT ACAGCAGCTC
    ATGGCCAAGG GCAGCGACAA ACGTCGGGGT GGCCCTCCAG GCCCGCCTAT CAACCGTGGC
    CTTCCACTTG TAGATGATGG TGGCTGGAAT ACAGTCCCCA TTAGCAAAGG CAGTCGCCCC
    ATTGACACCT CACGGCTCAC CAAGATCACA AAGCCTGGTT CCATTGATTC TAACAACCAG
    CTTTTTGCAC CTGGAGGACG ACTGAGTTGG GGCAAGGGCA GCAGTGGGGG CTCAGGAGCC
    AAGCCCTCAG ACACAGCATC GGAAGCTACT CGTCCAGCTA CCTTGAATCG CTTTTCTGCT
    CTTCAACAAA CATTACCCGC AGAGAACACA GATAACAGAC GTGTTGTACA GAGGAGTAGC
    TTGAGCCGGG AACGAGGTGA GAAAGCTGGG GACCGGGGAG ACCGACTAGA GCGGAGTGAG
    CGGGGAGGTG ACCGTGGGGA CCGACTTGAT CGTGCCAGAA CACCTGCCAC CAAGCGAAGT
    TTTAGCAAAG AAGTGGAGGA GCGAAGTAGA GAGCGGCCAT CCCAGCCTGA GGGACTCCGC
    AAGGCAGCTA GCCTCACAGA GGATCGTGGT CGGGATCCTG TGAAGCGGGA AGCCACTCTA
    CCTCCAGTGA GCCCTCCAAA GGCTGCGCTG TCTGTGGATG AGGTGGAGAA GAAATCTAAG
    GCCATCATTG AGGAATATCT CCATCTCAAT GACATGAAGG AGGCAGTACA GTGTGTCCAG
    GAGCTGGCTT CACCCTCCTT GCTCTTCATC TTCGTACGGC TTGGTATCGA GTCCACGCTG
    GAGCGTAGTA CCATTGCTCG TGAGCATATG GGGCGACTAC TGCACCAACT GCTCTGTGCG
    GGGCACCTCT CTACTGCCCA GTACTATCAA GGGCTGTATG AAACACTAGA ATTGGCTGAG
    GACATGGAAA TTGATATCCC TCATGTATGG CTTTACCTGG CAGAACTGAT TACACCTATT
    CTCCAGGAAG ATGGGGTGCC CATGGGAGAG CTCTTCAGGG AAATTACGAA GCCTCTGAGA
    CCCATGGGCA AAGCTACTTC TTTATTGCTG GAGATCCTGG GTCTCTTATG CAAGAGCATG
    GGTCCCAAAA AGGTGGGGAT GCTGTGGCGA GAGGCTGGGC TGAGCTGGAG GGAATTTCTA
    GCAGAGGGCC AAGACGTTGG CTCATTTGTG GCTGAAAAGA AGGTGGAATA TACCTTGGGA
    GAAGAATCTG AAGCTCCTGG CCAGAGGACA CTTGCCTTTG AGGAGCTTCG TAGGCAGCTA
    GAGAAGCTGC TGAAGGACGG CGGCAGTAAT CAGCGTGTGT TCGACTGGAT AGACGCCAAC
    CTAAATGAGC AGCAGATAGC ATCCAATACA TTAGTTCGAG CCCTCATGAC AACTGTCTGT
    TATTCTGCAA TTATCTTTGA GACTCCTCTC CGAGTGGATG TTCAAGTGTT GAAAGTGCGA
    GCAAGACTGC TGCAGAAATA CCTGTGTGAT GAGCAGAAGG AGCTACAAGC ACTCTATGCT
    CTCCAGGCCC TTGTAGTGAC CTTAGAACAG CCTGCCAACC TGCTTCGGAT GTTCTTTGAT
    GCTCTATATG ATGAGGACGT GGTGAAGGAA GACGCCTTCT ACAGCTGGGA GAGCAGCAAG
    GATCCTGCTG AACAGCAGGG CAAGGGTGTG GCCCTTAAAT CTGTCACAGC ATTCTTCAAT
    TGGCTTCGTG AGGCTGAGGA CGAGGAGTCT GATCACAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51986
    Clone ID Related Accession (Same CDS sequence) XM_006521935.1 , XM_006521936.2
    Accession Version XM_006521935.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006521998.1
    CDS249..5054
    Translation

    Target ORF information:

    RefSeq Version XM_006521935.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521935.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51986
    Clone ID Related Accession (Same CDS sequence) XM_006521935.1 , XM_006521936.2
    Accession Version XM_006521936.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4806bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521936.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006521999.1
    CDS137..4942
    Translation

    Target ORF information:

    RefSeq Version XM_006521936.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521936.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51987
    Clone ID Related Accession (Same CDS sequence) XM_006521938.1 , XM_006521939.1 , XM_006521938.2
    Accession Version XM_006521938.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522001.1
    CDS249..5033
    Translation

    Target ORF information:

    RefSeq Version XM_006521938.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521938.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51987
    Clone ID Related Accession (Same CDS sequence) XM_006521938.1 , XM_006521939.1 , XM_006521938.2
    Accession Version XM_006521939.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522002.1
    CDS192..4976
    Translation

    Target ORF information:

    RefSeq Version XM_006521939.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521939.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51989
    Clone ID Related Accession (Same CDS sequence) XM_006521942.1
    Accession Version XM_006521942.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT
    CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT
    GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG
    ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG
    GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG
    ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC
    CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA
    GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC
    CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT
    ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT
    GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA
    GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC
    ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG
    GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT
    GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA
    GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG
    AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA
    GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA
    GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC
    CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC
    CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC
    TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG
    TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC
    AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG
    CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT
    CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT
    GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGC AGGCTGGCCT GGGACCCAGG CGCTCTCAGC AGGGCCCACG AAAGGAAACT
    CGCAAGATCA TTTCCTCAGT GATAATGACT GAAGACATAA AACTAAATAA AGCAGAGAAG
    GCTTGGAAAC CCAGTAGCAA ACGGACAGCA GCTGATAAGG ATCGAGGGGA AGAGGATGCT
    GATGGAAGCA AGACCCAGGA CCTGTTCCGC AGGGTACGCT CCATCCTGAA TAAGCTGACA
    CCCCAGATGT TTCAGCAGCT GATGAAGCAG GTGACACAGC TGGCCATTGA CACGGAGGAA
    CGCCTCAAAG GAGTTATTGA CCTCATCTTT GAGAAAGCCA TTTCAGAGCC CAACTTCTCT
    GTGGCTTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG
    CCAACAGTAA CTGTGAATTT TCGAAAACTG TTGTTAAATC GGTGCCAGAA GGAATTTGAG
    AAAGACAAAG ATGATGATGA AGTTTTTGAA AAAAAGCAAA AAGAGATGGA TGAAGCTGCT
    ACGGCAGAAG AACGGGGACG CCTGAAAGAA GAGCTAGAAG AAGCCCGGGA CATAGCTAGG
    CGGCGCTCTT TAGGGAATAT CAAGTTTATT GGAGAGTTAT TCAAGTTGAA GATGTTAACA
    GAAGCAATAA TGCATGACTG TGTGGTCAAG CTACTTAAGA ACCATGATGA GGAGTCCCTG
    GAATGCCTCT GCCGCCTCCT CACCACTATA GGCAAAGACC TGGACTTTGC AAAAGCCAAG
    CCTCGAATGG ATCAGTATTT CAACCAAATG GAAAAAATCA TTAAAGAAAA GAAGACCTCA
    TCTCGCATCC GTTTTATGCT GCAGGATGTA CTCGATCTGC GGCAGAGTAA TTGGGTGCCA
    CGCCGAGGGG ATCAGGGTCC TAAGACTATT GATCAAATTC ACAAGGAGGC TGAGATGGAA
    GAGCACCGAG AACATATTAA AGTACAGCAG CTCATGGCCA AGGGCAGCGA CAAACGTCGG
    GGTGGCCCTC CAGGCCCGCC TATCAACCGT GGCCTTCCAC TTGTAGATGA TGGTGGCTGG
    AATACAGTCC CCATTAGCAA AGGCAGTCGC CCCATTGACA CCTCACGGCT CACCAAGATC
    ACAAAGCCTG GTTCCATTGA TTCTAACAAC CAGCTTTTTG CACCTGGAGG ACGACTGAGT
    TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA GCCAAGCCCT CAGACACAGC ATCGGAAGCT
    ACTCGTCCAG CTACCTTGAA TCGCTTTTCT GCTCTTCAAC AAACATTACC CGCAGAGAAC
    ACAGATAACA GACGTGTTGT ACAGAGGAGT AGCTTGAGCC GGGAACGAGG TGAGAAAGCT
    GGGGACCGGG GAGACCGACT AGAGCGGAGT GAGCGGGGAG GTGACCGTGG GGACCGACTT
    GATCGTGCCA GAACACCTGC CACCAAGCGA AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT
    AGAGAGCGGC CATCCCAGCC TGAGGGACTC CGCAAGGCAG CTAGCCTCAC AGAGGATCGT
    GGTCGGGATC CTGTGAAGCG GGAAGCCACT CTACCTCCAG TGAGCCCTCC AAAGGCTGCG
    CTGTCTGTGG ATGAGGTGGA GAAGAAATCT AAGGCCATCA TTGAGGAATA TCTCCATCTC
    AATGACATGA AGGAGGCAGT ACAGTGTGTC CAGGAGCTGG CTTCACCCTC CTTGCTCTTC
    ATCTTCGTAC GGCTTGGTAT CGAGTCCACG CTGGAGCGTA GTACCATTGC TCGTGAGCAT
    ATGGGGCGAC TACTGCACCA ACTGCTCTGT GCGGGGCACC TCTCTACTGC CCAGTACTAT
    CAAGGGCTGT ATGAAACACT AGAATTGGCT GAGGACATGG AAATTGATAT CCCTCATGTA
    TGGCTTTACC TGGCAGAACT GATTACACCT ATTCTCCAGG AAGATGGGGT GCCCATGGGA
    GAGCTCTTCA GGGAAATTAC GAAGCCTCTG AGACCCATGG GCAAAGCTAC TTCTTTATTG
    CTGGAGATCC TGGGTCTCTT ATGCAAGAGC ATGGGTCCCA AAAAGGTGGG GATGCTGTGG
    CGAGAGGCTG GGCTGAGCTG GAGGGAATTT CTAGCAGAGG GCCAAGACGT TGGCTCATTT
    GTGGCTGAAA AGAAGGTGGA ATATACCTTG GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG
    ACACTTGCCT TTGAGGAGCT TCGTAGGCAG CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT
    AATCAGCGTG TGTTCGACTG GATAGACGCC AACCTAAATG AGCAGCAGAT AGCATCCAAT
    ACATTAGTTC GAGCCCTCAT GACAACTGTC TGTTATTCTG CAATTATCTT TGAGACTCCT
    CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG CGAGCAAGAC TGCTGCAGAA ATACCTGTGT
    GATGAGCAGA AGGAGCTACA AGCACTCTAT GCTCTCCAGG CCCTTGTAGT GACCTTAGAA
    CAGCCTGCCA ACCTGCTTCG GATGTTCTTT GATGCTCTAT ATGATGAGGA CGTGGTGAAG
    GAAGACGCCT TCTACAGCTG GGAGAGCAGC AAGGATCCTG CTGAACAGCA GGGCAAGGGT
    GTGGCCCTTA AATCTGTCAC AGCATTCTTC AATTGGCTTC GTGAGGCTGA GGACGAGGAG
    TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522005.1
    CDS249..5003
    Translation

    Target ORF information:

    RefSeq Version XM_006521942.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521942.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGCAGTAC CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT
    CCAGGTGCAA GCCCTACCGA GTTTGGGACC TATGCTGGAG CCTATTACCC AGCCCAAGGT
    GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT GCCCCAGTTT TGATGAACCA GCCACCCCAG
    ATTGCTCCTA AGAGGGAACG GAAAACTATC CGAATTCGAG ACCCAAACCA AGGAGGGAAG
    GATATCACAG AAGAGATCAT GTCTGGGGCC CGCACTGCCT CCACACCCAC TCCTCCCCAG
    ACGGGAGGCA GTCTGGAGCC TCAACCCAAT GGGGAGTCGC CTCAGGTTGC TGTCATTATC
    CGGCCAGATG ACCGGTCGCA GGGAGCAGCC ATTGGGGGGC GGCCAGGACT GCCTGGCCCA
    GAGCATAGCC CTGGCACAGA ATCTCAGCCT TCGTCGCCTT CTCCAACCCC ATCACCACCC
    CCAATTTTGG AGCCGGGGTC TGAGTCTAAT CTTGGAGTCC TCTCTATTCC TGGGGACACT
    ATGACAACAG GGATGATACC AATGTCTGTA GAAGAATCGA CCCCTATCTC TTGTGAAACT
    GGGGAGCCGT ATTGCCTCTC TCCAGAACCC ACTCTTGCCG AACCCATACT GGAAGTAGAA
    GTGACACTCA GCAAACCCAT TCCAGAATCT GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC
    ACGGCCCTGG TGCCTCACAA GGTTGAAACT CATGAGCCCA ATGGCGTGAT CCCATCTGAG
    GATCTGGAAC CAGAGGTGGA GTCAAGCACA GAGCCAGCTC CTCCCCCTCT ATCACCTTGT
    GCTTCTGAAT CGCTCGTGCC CATTGCTCCA ACTGCCCAGC CTGAGGAACT GCTCAACGGA
    GCCCCCTCAC CACCAGCTGT GGATTTAAGC CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG
    AAGGTTTCAT CAGCAGCACT GGCCAGCATT CTCTCTCCTG CTCCACCTGT GGCTCCTTCA
    GATACTTCTC CTGCTCAGGA GGAAGAAATG GAAGAAGATG ACGATGACGA AGAAGGTGGA
    GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC GTCCCCCTTG ACAGTACTCC TGTCCCAGCC
    CAGCTGTCTC AGAATTTAGA GGTGGCAGCA GCTACCCAGG TGGCAGTGTC TGTGCCAAAG
    AGGAGACGGA AAATTAAAGA GCTAAATAAG AAGGAGGCTG TAGGAGACCT TCTAGATGCC
    TTCAAGGAGG TGGACCCAGC AGTACCAGAG GTAGAGAATC AGCCTCCCAC TGGCAGCAAC
    CCAAGCCCAG AGTCTGAGGG CAGCATGGTG CCCACACAGC CTGAGGAAAC AGAAGAAACC
    TGGGACTCTA AGGAAGACAA AATTCATAAT GCTGAGAACA TCCAGCCTGG GGAGCAGAAG
    TATGAGTACA AGTCAGATCA GTGGAAGCCT CTAAACCTTG AAGAAAAGAA GCGTTATGAC
    AGAGAATTCC TGCTGGGCTT TCAGTTCATC TTTGCCAGCA TGCAGAAGCC AGAAGGATTG
    CCCCATATCA CTGATGTGGT GCTGGACAAG GCCAATAAAA CACCACTTCG GCAACTGGAT
    CCCTCCAGAC TACCTGGCAT AAACTGTGGC CCAGATTTCA CTCCATCTTT TGCCAATCTT
    GGCCGACCAG CCCTCAGCAA CCGTGGGCCC CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC
    CGAGGGCCGC AGGCTGGCCT GGGACCCAGG CGCTCTCAGC AGGGCCCACG AAAGGAAACT
    CGCAAGATCA TTTCCTCAGT GATAATGACT GAAGACATAA AACTAAATAA AGCAGAGAAG
    GCTTGGAAAC CCAGTAGCAA ACGGACAGCA GCTGATAAGG ATCGAGGGGA AGAGGATGCT
    GATGGAAGCA AGACCCAGGA CCTGTTCCGC AGGGTACGCT CCATCCTGAA TAAGCTGACA
    CCCCAGATGT TTCAGCAGCT GATGAAGCAG GTGACACAGC TGGCCATTGA CACGGAGGAA
    CGCCTCAAAG GAGTTATTGA CCTCATCTTT GAGAAAGCCA TTTCAGAGCC CAACTTCTCT
    GTGGCTTATG CCAACATGTG CCGCTGCCTC ATGGCGCTGA AAGTGCCCAC TACAGAAAAG
    CCAACAGTAA CTGTGAATTT TCGAAAACTG TTGTTAAATC GGTGCCAGAA GGAATTTGAG
    AAAGACAAAG ATGATGATGA AGTTTTTGAA AAAAAGCAAA AAGAGATGGA TGAAGCTGCT
    ACGGCAGAAG AACGGGGACG CCTGAAAGAA GAGCTAGAAG AAGCCCGGGA CATAGCTAGG
    CGGCGCTCTT TAGGGAATAT CAAGTTTATT GGAGAGTTAT TCAAGTTGAA GATGTTAACA
    GAAGCAATAA TGCATGACTG TGTGGTCAAG CTACTTAAGA ACCATGATGA GGAGTCCCTG
    GAATGCCTCT GCCGCCTCCT CACCACTATA GGCAAAGACC TGGACTTTGC AAAAGCCAAG
    CCTCGAATGG ATCAGTATTT CAACCAAATG GAAAAAATCA TTAAAGAAAA GAAGACCTCA
    TCTCGCATCC GTTTTATGCT GCAGGATGTA CTCGATCTGC GGCAGAGTAA TTGGGTGCCA
    CGCCGAGGGG ATCAGGGTCC TAAGACTATT GATCAAATTC ACAAGGAGGC TGAGATGGAA
    GAGCACCGAG AACATATTAA AGTACAGCAG CTCATGGCCA AGGGCAGCGA CAAACGTCGG
    GGTGGCCCTC CAGGCCCGCC TATCAACCGT GGCCTTCCAC TTGTAGATGA TGGTGGCTGG
    AATACAGTCC CCATTAGCAA AGGCAGTCGC CCCATTGACA CCTCACGGCT CACCAAGATC
    ACAAAGCCTG GTTCCATTGA TTCTAACAAC CAGCTTTTTG CACCTGGAGG ACGACTGAGT
    TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA GCCAAGCCCT CAGACACAGC ATCGGAAGCT
    ACTCGTCCAG CTACCTTGAA TCGCTTTTCT GCTCTTCAAC AAACATTACC CGCAGAGAAC
    ACAGATAACA GACGTGTTGT ACAGAGGAGT AGCTTGAGCC GGGAACGAGG TGAGAAAGCT
    GGGGACCGGG GAGACCGACT AGAGCGGAGT GAGCGGGGAG GTGACCGTGG GGACCGACTT
    GATCGTGCCA GAACACCTGC CACCAAGCGA AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT
    AGAGAGCGGC CATCCCAGCC TGAGGGACTC CGCAAGGCAG CTAGCCTCAC AGAGGATCGT
    GGTCGGGATC CTGTGAAGCG GGAAGCCACT CTACCTCCAG TGAGCCCTCC AAAGGCTGCG
    CTGTCTGTGG ATGAGGTGGA GAAGAAATCT AAGGCCATCA TTGAGGAATA TCTCCATCTC
    AATGACATGA AGGAGGCAGT ACAGTGTGTC CAGGAGCTGG CTTCACCCTC CTTGCTCTTC
    ATCTTCGTAC GGCTTGGTAT CGAGTCCACG CTGGAGCGTA GTACCATTGC TCGTGAGCAT
    ATGGGGCGAC TACTGCACCA ACTGCTCTGT GCGGGGCACC TCTCTACTGC CCAGTACTAT
    CAAGGGCTGT ATGAAACACT AGAATTGGCT GAGGACATGG AAATTGATAT CCCTCATGTA
    TGGCTTTACC TGGCAGAACT GATTACACCT ATTCTCCAGG AAGATGGGGT GCCCATGGGA
    GAGCTCTTCA GGGAAATTAC GAAGCCTCTG AGACCCATGG GCAAAGCTAC TTCTTTATTG
    CTGGAGATCC TGGGTCTCTT ATGCAAGAGC ATGGGTCCCA AAAAGGTGGG GATGCTGTGG
    CGAGAGGCTG GGCTGAGCTG GAGGGAATTT CTAGCAGAGG GCCAAGACGT TGGCTCATTT
    GTGGCTGAAA AGAAGGTGGA ATATACCTTG GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG
    ACACTTGCCT TTGAGGAGCT TCGTAGGCAG CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT
    AATCAGCGTG TGTTCGACTG GATAGACGCC AACCTAAATG AGCAGCAGAT AGCATCCAAT
    ACATTAGTTC GAGCCCTCAT GACAACTGTC TGTTATTCTG CAATTATCTT TGAGACTCCT
    CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG CGAGCAAGAC TGCTGCAGAA ATACCTGTGT
    GATGAGCAGA AGGAGCTACA AGCACTCTAT GCTCTCCAGG CCCTTGTAGT GACCTTAGAA
    CAGCCTGCCA ACCTGCTTCG GATGTTCTTT GATGCTCTAT ATGATGAGGA CGTGGTGAAG
    GAAGACGCCT TCTACAGCTG GGAGAGCAGC AAGGATCCTG CTGAACAGCA GGGCAAGGGT
    GTGGCCCTTA AATCTGTCAC AGCATTCTTC AATTGGCTTC GTGAGGCTGA GGACGAGGAG
    TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51990
    Clone ID Related Accession (Same CDS sequence) XM_030249025.1 , XM_017316919.1 , XM_006521943.1
    Accession Version XM_017316919.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017172408.1
    CDS654..5339
    Translation

    Target ORF information:

    RefSeq Version XM_017316919.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017316919.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51990
    Clone ID Related Accession (Same CDS sequence) XM_030249025.1 , XM_017316919.1 , XM_006521943.1
    Accession Version XM_006521943.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4686bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522006.1
    CDS275..4960
    Translation

    Target ORF information:

    RefSeq Version XM_006521943.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521943.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGGGGCG TTCCACATAT
    GTTGTCCCAA CACAGCAGTA CCCTGTGCAG CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA
    AGCCCTACCG AGTTTGGGAC CTATGCTGGA GCCTATTACC CAGCCCAAGG TGTGCAGCAG
    TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT TTGATGAACC AGCCACCCCA GATTGCTCCT
    AAGAGGGAAC GGAAAACTAT CCGAATTCGA GACCCAAACC AAGGAGGGAA GGATATCACA
    GAAGAGATCA TGTCTGGGGC CCGCACTGCC TCCACACCCA CTCCTCCCCA GACGGGAGGC
    AGTCTGGAGC CTCAACCCAA TGGGGAGTCG CCTCAGGTTG CTGTCATTAT CCGGCCAGAT
    GACCGGTCGC AGGGAGCAGC CATTGGGGGG CGGCCAGGAC TGCCTGGCCC AGAGCATAGC
    CCTGGCACAG AATCTCAGCC TTCGTCGCCT TCTCCAACCC CATCACCACC CCCAATTTTG
    GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC CTCTCTATTC CTGGGGACAC TATGACAACA
    GGGATGATAC CAATGTCTGT AGAAGAATCG ACCCCTATCT CTTGTGAAAC TGGGGAGCCG
    TATTGCCTCT CTCCAGAACC CACTCTTGCC GAACCCATAC TGGAAGTAGA AGTGACACTC
    AGCAAACCCA TTCCAGAATC TGAGTTCTCT TCCAGTCCTC TCCAGGTTTC CACGGCCCTG
    GTGCCTCACA AGGTTGAAAC TCATGAGCCC AATGGCGTGA TCCCATCTGA GGATCTGGAA
    CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT CCTCCCCCTC TATCACCTTG TGCTTCTGAA
    TCGCTCGTGC CCATTGCTCC AACTGCCCAG CCTGAGGAAC TGCTCAACGG AGCCCCCTCA
    CCACCAGCTG TGGATTTAAG CCCAGTCAGT GAGCCAGAGG AACAGGCCAA GAAGGTTTCA
    TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT GCTCCACCTG TGGCTCCTTC AGATACTTCT
    CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT GACGATGACG AAGAAGGTGG AGAAGCTGAG
    AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT GACAGTACTC CTGTCCCAGC CCAGCTGTCT
    CAGAATTTAG AGGTGGCAGC AGCTACCCAG GTGGCAGTGT CTGTGCCAAA GAGGAGACGG
    AAAATTAAAG AGCTAAATAA GAAGGAGGCT GTAGGAGACC TTCTAGATGC CTTCAAGGAG
    GTGGACCCAG CAGTACCAGA GGTAGAGAAT CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA
    GAGTCTGAGG GCAGCATGGT GCCCACACAG CCTGAGGAAA CAGAAGAAAC CTGGGACTCT
    AAGGAAGACA AAATTCATAA TGCTGAGAAC ATCCAGCCTG GGGAGCAGAA GTATGAGTAC
    AAGTCAGATC AGTGGAAGCC TCTAAACCTT GAAGAAAAGA AGCGTTATGA CAGAGAATTC
    CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC ATGCAGAAGC CAGAAGGATT GCCCCATATC
    ACTGATGTGG TGCTGGACAA GGCCAATAAA ACACCACTTC GGCAACTGGA TCCCTCCAGA
    CTACCTGGCA TAAACTGTGG CCCAGATTTC ACTCCATCTT TTGCCAATCT TGGCCGACCA
    GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG
    CAGGCTGGCC TGGGACCCAG GCGCTCTCAG CAGGGCCCAC GAAAGGAAAC TCGCAAGATC
    ATTTCCTCAG TGATAATGAC TGAAGACATA AAACTAAATA AAGCAGAGAA GGCTTGGAAA
    CCCAGTAGCA AACGGACAGC AGCTGATAAG GATCGAGGGG AAGAGGATGC TGATGGAAGC
    AAGACCCAGG ACCTGTTCCG CAGGGTACGC TCCATCCTGA ATAAGCTGAC ACCCCAGATG
    TTTCAGCAGC TGATGAAGCA GGTGACACAG CTGGCCATTG ACACGGAGGA ACGCCTCAAA
    GGAGTTATTG ACCTCATCTT TGAGAAAGCC ATTTCAGAGC CCAACTTCTC TGTGGCTTAT
    GCCAACATGT GCCGCTGCCT CATGGCGCTG AAAGTGCCCA CTACAGAAAA GCCAACAGTA
    ACTGTGAATT TTCGAAAACT GTTGTTAAAT CGGTGCCAGA AGGAATTTGA GAAAGACAAA
    GATGATGATG AAGTTTTTGA AAAAAAGCAA AAAGAGATGG ATGAAGCTGC TACGGCAGAA
    GAACGGGGAC GCCTGAAAGA AGAGCTAGAA GAAGCCCGGG ACATAGCTAG GCGGCGCTCT
    TTAGGGAATA TCAAGTTTAT TGGAGAGTTA TTCAAGTTGA AGATGTTAAC AGAAGCAATA
    ATGCATGACT GTGTGGTCAA GCTACTTAAG AACCATGATG AGGAGTCCCT GGAATGCCTC
    TGCCGCCTCC TCACCACTAT AGGCAAAGAC CTGGACTTTG CAAAAGCCAA GCCTCGAATG
    GATCAGTATT TCAACCAAAT GGAAAAAATC ATTAAAGAAA AGAAGACCTC ATCTCGCATC
    CGTTTTATGC TGCAGGATGT ACTCGATCTG CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG
    GATCAGGGTC CTAAGACTAT TGATCAAATT CACAAGGAGG CTGAGATGGA AGAGCACCGA
    GAACATATTA AAGTACAGCA GCTCATGGCC AAGGGCAGCG ACAAACGTCG GGGTGGCCCT
    CCAGGCCCGC CTATCAACCG TGGCCTTCCA CTTGTAGATG ATGGTGGCTG GAATACAGTC
    CCCATTAGCA AAGGCAGTCG CCCCATTGAC ACCTCACGGC TCACCAAGAT CACAAAGCCT
    GGTTCCATTG ATTCTAACAA CCAGCTTTTT GCACCTGGAG GACGACTGAG TTGGGGCAAG
    GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC TCAGACACAG CATCGGAAGC TACTCGTCCA
    GCTACCTTGA ATCGCTTTTC TGCTCTTCAA CAAACATTAC CCGCAGAGAA CACAGATAAC
    AGACGTGTTG TACAGAGGAG TAGCTTGAGC CGGGAACGAG GTGAGAAAGC TGGGGACCGG
    GGAGACCGAC TAGAGCGGAG TGAGCGGGGA GGTGACCGTG GGGACCGACT TGATCGTGCC
    AGAACACCTG CCACCAAGCG AAGTTTTAGC AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG
    CCATCCCAGC CTGAGGGACT CCGCAAGGCA GCTAGCCTCA CAGAGGATCG TGGTCGGGAT
    CCTGTGAAGC GGGAAGCCAC TCTACCTCCA GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG
    GATGAGGTGG AGAAGAAATC TAAGGCCATC ATTGAGGAAT ATCTCCATCT CAATGACATG
    AAGGAGGCAG TACAGTGTGT CCAGGAGCTG GCTTCACCCT CCTTGCTCTT CATCTTCGTA
    CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT AGTACCATTG CTCGTGAGCA TATGGGGCGA
    CTACTGCACC AACTGCTCTG TGCGGGGCAC CTCTCTACTG CCCAGTACTA TCAAGGGCTG
    TATGAAACAC TAGAATTGGC TGAGGACATG GAAATTGATA TCCCTCATGT ATGGCTTTAC
    CTGGCAGAAC TGATTACACC TATTCTCCAG GAAGATGGGG TGCCCATGGG AGAGCTCTTC
    AGGGAAATTA CGAAGCCTCT GAGACCCATG GGCAAAGCTA CTTCTTTATT GCTGGAGATC
    CTGGGTCTCT TATGCAAGAG CATGGGTCCC AAAAAGGTGG GGATGCTGTG GCGAGAGGCT
    GGGCTGAGCT GGAGGGAATT TCTAGCAGAG GGCCAAGACG TTGGCTCATT TGTGGCTGAA
    AAGAAGGTGG AATATACCTT GGGAGAAGAA TCTGAAGCTC CTGGCCAGAG GACACTTGCC
    TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG CTGCTGAAGG ACGGCGGCAG TAATCAGCGT
    GTGTTCGACT GGATAGACGC CAACCTAAAT GAGCAGCAGA TAGCATCCAA TACATTAGTT
    CGAGCCCTCA TGACAACTGT CTGTTATTCT GCAATTATCT TTGAGACTCC TCTCCGAGTG
    GATGTTCAAG TGTTGAAAGT GCGAGCAAGA CTGCTGCAGA AATACCTGTG TGATGAGCAG
    AAGGAGCTAC AAGCACTCTA TGCTCTCCAG GCCCTTGTAG TGACCTTAGA ACAGCCTGCC
    AACCTGCTTC GGATGTTCTT TGATGCTCTA TATGATGAGG ACGTGGTGAA GGAAGACGCC
    TTCTACAGCT GGGAGAGCAG CAAGGATCCT GCTGAACAGC AGGGCAAGGG TGTGGCCCTT
    AAATCTGTCA CAGCATTCTT CAATTGGCTT CGTGAGGCTG AGGACGAGGA GTCTGATCAC
    AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51991
    Clone ID Related Accession (Same CDS sequence) XM_006521944.3 , XM_030249026.1
    Accession Version XM_006521944.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006521944.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522007.1
    CDS275..4939
    Translation

    Target ORF information:

    RefSeq Version XM_006521944.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521944.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGCAC TTCTACCCTA GCCGGGCCCA GCCCCCTAGC 
    AGTGCAGCCT CCCGAGTGCA GAGTGCAGCC CCTGCCCGTC CTGGCCCAGC TCCCCATGTC
    TACCCTGCTG GATCCCAAGT AATGATGATC CCTTCCCAGA TCTCCTACTC AGCCTCCCAA
    GGAGCCTACT ATATCCCTGG ACAGGGGCGT TCCACATATG TTGTCCCAAC ACAGCAGTAC
    CCTGTGCAGC CAGGAGCCCC AGGCTTCTAT CCAGGTGCAA GCCCTACCGA GTTTGGGACC
    TATGCTGGAG CCTATTACCC AGCCCAAGGT GTGCAGCAGT TTCCTGCTAG TGTGGCTCCT
    GCCCCAGTTT TGATGAACCA GCCACCCCAG ATTGCTCCTA AGAGGGAACG GAAAACTATC
    CGAATTCGAG ACCCAAACCA AGGAGGGAAG GATATCACAG AAGAGATCAT GTCTGGGGCC
    CGCACTGCCT CCACACCCAC TCCTCCCCAG ACGGGAGGCA GTCTGGAGCC TCAACCCAAT
    GGGGAGTCGC CTCAGGTTGC TGTCATTATC CGGCCAGATG ACCGGTCGCA GGGAGCAGCC
    ATTGGGGGGC GGCCAGGACT GCCTGGCCCA GAGCATAGCC CTGGCACAGA ATCTCAGCCT
    TCGTCGCCTT CTCCAACCCC ATCACCACCC CCAATTTTGG AGCCGGGGTC TGAGTCTAAT
    CTTGGAGTCC TCTCTATTCC TGGGGACACT ATGACAACAG GGATGATACC AATGTCTGTA
    GAAGAATCGA CCCCTATCTC TTGTGAAACT GGGGAGCCGT ATTGCCTCTC TCCAGAACCC
    ACTCTTGCCG AACCCATACT GGAAGTAGAA GTGACACTCA GCAAACCCAT TCCAGAATCT
    GAGTTCTCTT CCAGTCCTCT CCAGGTTTCC ACGGCCCTGG TGCCTCACAA GGTTGAAACT
    CATGAGCCCA ATGGCGTGAT CCCATCTGAG GATCTGGAAC CAGAGGTGGA GTCAAGCACA
    GAGCCAGCTC CTCCCCCTCT ATCACCTTGT GCTTCTGAAT CGCTCGTGCC CATTGCTCCA
    ACTGCCCAGC CTGAGGAACT GCTCAACGGA GCCCCCTCAC CACCAGCTGT GGATTTAAGC
    CCAGTCAGTG AGCCAGAGGA ACAGGCCAAG AAGGTTTCAT CAGCAGCACT GGCCAGCATT
    CTCTCTCCTG CTCCACCTGT GGCTCCTTCA GATACTTCTC CTGCTCAGGA GGAAGAAATG
    GAAGAAGATG ACGATGACGA AGAAGGTGGA GAAGCTGAGA GTGAGAAGGG AGGAGAGGAC
    GTCCCCCTTG ACAGTACTCC TGTCCCAGCC CAGCTGTCTC AGAATTTAGA GGTGGCAGCA
    GCTACCCAGG TGGCAGTGTC TGTGCCAAAG AGGAGACGGA AAATTAAAGA GCTAAATAAG
    AAGGAGGCTG TAGGAGACCT TCTAGATGCC TTCAAGGAGG TGGACCCAGC AGTACCAGAG
    GTAGAGAATC AGCCTCCCAC TGGCAGCAAC CCAAGCCCAG AGTCTGAGGG CAGCATGGTG
    CCCACACAGC CTGAGGAAAC AGAAGAAACC TGGGACTCTA AGGAAGACAA AATTCATAAT
    GCTGAGAACA TCCAGCCTGG GGAGCAGAAG TATGAGTACA AGTCAGATCA GTGGAAGCCT
    CTAAACCTTG AAGAAAAGAA GCGTTATGAC AGAGAATTCC TGCTGGGCTT TCAGTTCATC
    TTTGCCAGCA TGCAGAAGCC AGAAGGATTG CCCCATATCA CTGATGTGGT GCTGGACAAG
    GCCAATAAAA CACCACTTCG GCAACTGGAT CCCTCCAGAC TACCTGGCAT AAACTGTGGC
    CCAGATTTCA CTCCATCTTT TGCCAATCTT GGCCGACCAG CCCTCAGCAA CCGTGGGCCC
    CCGAGGGGTG GGCCAGGTGG GGAGCTGCCC CGAGGGCCGC AGGCTGGCCT GGGACCCAGG
    CGCTCTCAGC AGGGCCCACG AAAGGAAACT CGCAAGATCA TTTCCTCAGT GATAATGACT
    GAAGACATAA AACTAAATAA AGCAGAGAAG GCTTGGAAAC CCAGTAGCAA ACGGACAGCA
    GCTGATAAGG ATCGAGGGGA AGAGGATGCT GATGGAAGCA AGACCCAGGA CCTGTTCCGC
    AGGGTACGCT CCATCCTGAA TAAGCTGACA CCCCAGATGT TTCAGCAGCT GATGAAGCAG
    GTGACACAGC TGGCCATTGA CACGGAGGAA CGCCTCAAAG GAGTTATTGA CCTCATCTTT
    GAGAAAGCCA TTTCAGAGCC CAACTTCTCT GTGGCTTATG CCAACATGTG CCGCTGCCTC
    ATGGCGCTGA AAGTGCCCAC TACAGAAAAG CCAACAGTAA CTGTGAATTT TCGAAAACTG
    TTGTTAAATC GGTGCCAGAA GGAATTTGAG AAAGACAAAG ATGATGATGA AGTTTTTGAA
    AAAAAGCAAA AAGAGATGGA TGAAGCTGCT ACGGCAGAAG AACGGGGACG CCTGAAAGAA
    GAGCTAGAAG AAGCCCGGGA CATAGCTAGG CGGCGCTCTT TAGGGAATAT CAAGTTTATT
    GGAGAGTTAT TCAAGTTGAA GATGTTAACA GAAGCAATAA TGCATGACTG TGTGGTCAAG
    CTACTTAAGA ACCATGATGA GGAGTCCCTG GAATGCCTCT GCCGCCTCCT CACCACTATA
    GGCAAAGACC TGGACTTTGC AAAAGCCAAG CCTCGAATGG ATCAGTATTT CAACCAAATG
    GAAAAAATCA TTAAAGAAAA GAAGACCTCA TCTCGCATCC GTTTTATGCT GCAGGATGTA
    CTCGATCTGC GGCAGAGTAA TTGGGTGCCA CGCCGAGGGG ATCAGGGTCC TAAGACTATT
    GATCAAATTC ACAAGGAGGC TGAGATGGAA GAGCACCGAG AACATATTAA AGTACAGCAG
    CTCATGGCCA AGGGCAGCGA CAAACGTCGG GGTGGCCCTC CAGGCCCGCC TATCAACCGT
    GGCCTTCCAC TTGTAGATGA TGGTGGCTGG AATACAGTCC CCATTAGCAA AGGCAGTCGC
    CCCATTGACA CCTCACGGCT CACCAAGATC ACAAAGCCTG GTTCCATTGA TTCTAACAAC
    CAGCTTTTTG CACCTGGAGG ACGACTGAGT TGGGGCAAGG GCAGCAGTGG GGGCTCAGGA
    GCCAAGCCCT CAGACACAGC ATCGGAAGCT ACTCGTCCAG CTACCTTGAA TCGCTTTTCT
    GCTCTTCAAC AAACATTACC CGCAGAGAAC ACAGATAACA GACGTGTTGT ACAGAGGAGT
    AGCTTGAGCC GGGAACGAGG TGAGAAAGCT GGGGACCGGG GAGACCGACT AGAGCGGAGT
    GAGCGGGGAG GTGACCGTGG GGACCGACTT GATCGTGCCA GAACACCTGC CACCAAGCGA
    AGTTTTAGCA AAGAAGTGGA GGAGCGAAGT AGAGAGCGGC CATCCCAGCC TGAGGGACTC
    CGCAAGGCAG CTAGCCTCAC AGAGGATCGT GGTCGGGATC CTGTGAAGCG GGAAGCCACT
    CTACCTCCAG TGAGCCCTCC AAAGGCTGCG CTGTCTGTGG ATGAGGTGGA GAAGAAATCT
    AAGGCCATCA TTGAGGAATA TCTCCATCTC AATGACATGA AGGAGGCAGT ACAGTGTGTC
    CAGGAGCTGG CTTCACCCTC CTTGCTCTTC ATCTTCGTAC GGCTTGGTAT CGAGTCCACG
    CTGGAGCGTA GTACCATTGC TCGTGAGCAT ATGGGGCGAC TACTGCACCA ACTGCTCTGT
    GCGGGGCACC TCTCTACTGC CCAGTACTAT CAAGGGCTGT ATGAAACACT AGAATTGGCT
    GAGGACATGG AAATTGATAT CCCTCATGTA TGGCTTTACC TGGCAGAACT GATTACACCT
    ATTCTCCAGG AAGATGGGGT GCCCATGGGA GAGCTCTTCA GGGAAATTAC GAAGCCTCTG
    AGACCCATGG GCAAAGCTAC TTCTTTATTG CTGGAGATCC TGGGTCTCTT ATGCAAGAGC
    ATGGGTCCCA AAAAGGTGGG GATGCTGTGG CGAGAGGCTG GGCTGAGCTG GAGGGAATTT
    CTAGCAGAGG GCCAAGACGT TGGCTCATTT GTGGCTGAAA AGAAGGTGGA ATATACCTTG
    GGAGAAGAAT CTGAAGCTCC TGGCCAGAGG ACACTTGCCT TTGAGGAGCT TCGTAGGCAG
    CTAGAGAAGC TGCTGAAGGA CGGCGGCAGT AATCAGCGTG TGTTCGACTG GATAGACGCC
    AACCTAAATG AGCAGCAGAT AGCATCCAAT ACATTAGTTC GAGCCCTCAT GACAACTGTC
    TGTTATTCTG CAATTATCTT TGAGACTCCT CTCCGAGTGG ATGTTCAAGT GTTGAAAGTG
    CGAGCAAGAC TGCTGCAGAA ATACCTGTGT GATGAGCAGA AGGAGCTACA AGCACTCTAT
    GCTCTCCAGG CCCTTGTAGT GACCTTAGAA CAGCCTGCCA ACCTGCTTCG GATGTTCTTT
    GATGCTCTAT ATGATGAGGA CGTGGTGAAG GAAGACGCCT TCTACAGCTG GGAGAGCAGC
    AAGGATCCTG CTGAACAGCA GGGCAAGGGT GTGGCCCTTA AATCTGTCAC AGCATTCTTC
    AATTGGCTTC GTGAGGCTGA GGACGAGGAG TCTGATCACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51992
    Clone ID Related Accession (Same CDS sequence) XM_006521945.1
    Accession Version XM_006521945.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG
    CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA
    GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT
    TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA
    GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC
    TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG
    CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG
    CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT
    TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC
    CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG
    ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC
    GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT
    TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC
    AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT
    CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG
    CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT
    GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT
    GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT
    GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT
    GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG
    GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT
    GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT
    CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG
    CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC
    ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT
    GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC
    ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA
    ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC
    ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT
    GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG
    CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA
    AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG
    GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC
    TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG
    CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC
    ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG
    AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT
    CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA
    AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA
    GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA
    TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG
    AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC
    CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC
    ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG
    CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT
    CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC
    AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA
    CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC
    ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT
    GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC
    TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA
    CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC
    CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA
    GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC
    AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA
    GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA
    GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC
    ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG
    GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT
    AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC
    CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG
    GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG
    GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG
    GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC
    AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG
    GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA
    TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG
    CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT
    GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT
    GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA
    CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG
    GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA
    TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT
    GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT
    CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522008.1
    CDS218..4873
    Translation

    Target ORF information:

    RefSeq Version XM_006521945.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521945.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT 
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG
    CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA
    GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT
    TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA
    GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC
    TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG
    CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG
    CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT
    TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC
    CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG
    ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC
    GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT
    TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC
    AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT
    CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG
    CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT
    GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT
    GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT
    GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT
    GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG
    GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT
    GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT
    CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG
    CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC
    ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT
    GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC
    ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA
    ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC
    ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT
    GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG
    CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA
    AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG
    GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC
    TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG
    CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC
    ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG
    AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT
    CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA
    AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA
    GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA
    TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG
    AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC
    CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC
    ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG
    CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT
    CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC
    AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA
    CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC
    ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT
    GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC
    TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA
    CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC
    CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA
    GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC
    AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA
    GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA
    GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC
    ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG
    GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT
    AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC
    CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG
    GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG
    GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG
    GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC
    AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG
    GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA
    TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG
    CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT
    GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT
    GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA
    CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG
    GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA
    TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT
    GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT
    CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu51993
    Clone ID Related Accession (Same CDS sequence) XM_006521946.1
    Accession Version XM_006521946.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
    CAGGGGCGTT CCACATATGT TGTCCCAACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA
    GGCTTCTATC CAGGTGCAAG CCCTACCGAG TTTGGGACCT ATGCTGGAGC CTATTACCCA
    GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT GTGGCTCCTG CCCCAGTTTT GATGAACCAG
    CCACCCCAGA TTGCTCCTAA GAGGGAACGG AAAACTATCC GAATTCGAGA CCCAAACCAA
    GGAGGGAAGG ATATCACAGA AGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACT
    CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT CAACCCAATG GGGAGTCGCC TCAGGTTGCT
    GTCATTATCC GGCCAGATGA CCGGTCGCAG GGAGCAGCCA TTGGGGGGCG GCCAGGACTG
    CCTGGCCCAG AGCATAGCCC TGGCACAGAA TCTCAGCCTT CGTCGCCTTC TCCAACCCCA
    TCACCACCCC CAATTTTGGA GCCGGGGTCT GAGTCTAATC TTGGAGTCCT CTCTATTCCT
    GGGGACACTA TGACAACAGG GATGATACCA ATGTCTGTAG AAGAATCGAC CCCTATCTCT
    TGTGAAACTG GGGAGCCGTA TTGCCTCTCT CCAGAACCCA CTCTTGCCGA ACCCATACTG
    GAAGTAGAAG TGACACTCAG CAAACCCATT CCAGAATCTG AGTTCTCTTC CAGTCCTCTC
    CAGGTTTCCA CGGCCCTGGT GCCTCACAAG GTTGAAACTC ATGAGCCCAA TGGCGTGATC
    CCATCTGAGG ATCTGGAACC AGAGGTGGAG TCAAGCACAG AGCCAGCTCC TCCCCCTCTA
    TCACCTTGTG CTTCTGAATC GCTCGTGCCC ATTGCTCCAA CTGCCCAGCC TGAGGAACTG
    CTCAACGGAG CCCCCTCACC ACCAGCTGTG GATTTAAGCC CAGTCAGTGA GCCAGAGGAA
    CAGGCCAAGA AGGTTTCATC AGCAGCACTG GCCAGCATTC TCTCTCCTGC TCCACCTGTG
    GCTCCTTCAG ATACTTCTCC TGCTCAGGAG GAAGAAATGG AAGAAGATGA CGATGACGAA
    GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA GGAGAGGACG TCCCCCTTGA CAGTACTCCT
    GTCCCAGCCC AGCTGTCTCA GAATTTAGAG GTGGCAGCAG CTACCCAGGT GGCAGTGTCT
    GTGCCAAAGA GGAGACGGAA AATTAAAGAG CTAAATAAGA AGGAGGCTGT AGGAGACCTT
    CTAGATGCCT TCAAGGAGGT GGACCCAGCA GTACCAGAGG TAGAGAATCA GCCTCCCACT
    GGCAGCAACC CAAGCCCAGA GTCTGAGGGC AGCATGGTGC CCACACAGCC TGAGGAAACA
    GAAGAAACCT GGGACTCTAA GGAAGACAAA ATTCATAATG CTGAGAACAT CCAGCCTGGG
    GAGCAGAAGT ATGAGTACAA GTCAGATCAG TGGAAGCCTC TAAACCTTGA AGAAAAGAAG
    CGTTATGACA GAGAATTCCT GCTGGGCTTT CAGTTCATCT TTGCCAGCAT GCAGAAGCCA
    GAAGGATTGC CCCATATCAC TGATGTGGTG CTGGACAAGG CCAATAAAAC ACCACTTCGG
    CAACTGGATC CCTCCAGACT ACCTGGCATA AACTGTGGCC CAGATTTCAC TCCATCTTTT
    GCCAATCTTG GCCGACCAGC CCTCAGCAAC CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG
    GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG GGACCCAGGC GCTCTCAGCA GGGCCCACGA
    AAGGAAACTC GCAAGATCAT TTCCTCAGTG ATAATGACTG AAGACATAAA ACTAAATAAA
    GCAGAGAAGG CTTGGAAACC CAGTAGCAAA CGGACAGCAG CTGATAAGGA TCGAGGGGAA
    GAGGATGCTG ATGGAAGCAA GACCCAGGAC CTGTTCCGCA GGGTACGCTC CATCCTGAAT
    AAGCTGACAC CCCAGATGTT TCAGCAGCTG ATGAAGCAGG TGACACAGCT GGCCATTGAC
    ACGGAGGAAC GCCTCAAAGG AGTTATTGAC CTCATCTTTG AGAAAGCCAT TTCAGAGCCC
    AACTTCTCTG TGGCTTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT
    ACAGAAAAGC CAACAGTAAC TGTGAATTTT CGAAAACTGT TGTTAAATCG GTGCCAGAAG
    GAATTTGAGA AAGACAAAGA TGATGATGAA GTTTTTGAAA AAAAGCAAAA AGAGATGGAT
    GAAGCTGCTA CGGCAGAAGA ACGGGGACGC CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC
    ATAGCTAGGC GGCGCTCTTT AGGGAATATC AAGTTTATTG GAGAGTTATT CAAGTTGAAG
    ATGTTAACAG AAGCAATAAT GCATGACTGT GTGGTCAAGC TACTTAAGAA CCATGATGAG
    GAGTCCCTGG AATGCCTCTG CCGCCTCCTC ACCACTATAG GCAAAGACCT GGACTTTGCA
    AAAGCCAAGC CTCGAATGGA TCAGTATTTC AACCAAATGG AAAAAATCAT TAAAGAAAAG
    AAGACCTCAT CTCGCATCCG TTTTATGCTG CAGGATGTAC TCGATCTGCG GCAGAGTAAT
    TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT AAGACTATTG ATCAAATTCA CAAGGAGGCT
    GAGATGGAAG AGCACCGAGA ACATATTAAA GTACAGCAGC TCATGGCCAA GGGCAGCGAC
    AAACGTCGGG GTGGCCCTCC AGGCCCGCCT ATCAACCGTG GCCTTCCACT TGTAGATGAT
    GGTGGCTGGA ATACAGTCCC CATTAGCAAA GGCAGTCGCC CCATTGACAC CTCACGGCTC
    ACCAAGATCA CAAAGCCTGG TTCCATTGAT TCTAACAACC AGCTTTTTGC ACCTGGAGGA
    CGACTGAGTT GGGGCAAGGG CAGCAGTGGG GGCTCAGGAG CCAAGCCCTC AGACACAGCA
    TCGGAAGCTA CTCGTCCAGC TACCTTGAAT CGCTTTTCTG CTCTTCAACA AACATTACCC
    GCAGAGAACA CAGATAACAG ACGTGTTGTA CAGAGGAGTA GCTTGAGCCG GGAACGAGGT
    GAGAAAGCTG GGGACCGGGG AGACCGACTA GAGCGGAGTG AGCGGGGAGG TGACCGTGGG
    GACCGACTTG ATCGTGCCAG AACACCTGCC ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG
    GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT GAGGGACTCC GCAAGGCAGC TAGCCTCACA
    GAGGATCGTG GTCGGGATCC TGTGAAGCGG GAAGCCACTC TACCTCCAGT GAGCCCTCCA
    AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG AAGAAATCTA AGGCCATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA GGAGGCAGTA CAGTGTGTCC AGGAGCTGGC TTCACCCTCC
    TTGCTCTTCA TCTTCGTACG GCTTGGTATC GAGTCCACGC TGGAGCGTAG TACCATTGCT
    CGTGAGCATA TGGGGCGACT ACTGCACCAA CTGCTCTGTG CGGGGCACCT CTCTACTGCC
    CAGTACTATC AAGGGCTGTA TGAAACACTA GAATTGGCTG AGGACATGGA AATTGATATC
    CCTCATGTAT GGCTTTACCT GGCAGAACTG ATTACACCTA TTCTCCAGGA AGATGGGGTG
    CCCATGGGAG AGCTCTTCAG GGAAATTACG AAGCCTCTGA GACCCATGGG CAAAGCTACT
    TCTTTATTGC TGGAGATCCT GGGTCTCTTA TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG
    ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG AGGGAATTTC TAGCAGAGGG CCAAGACGTT
    GGCTCATTTG TGGCTGAAAA GAAGGTGGAA TATACCTTGG GAGAAGAATC TGAAGCTCCT
    GGCCAGAGGA CACTTGCCTT TGAGGAGCTT CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC
    GGCGGCAGTA ATCAGCGTGT GTTCGACTGG ATAGACGCCA ACCTAAATGA GCAGCAGATA
    GCATCCAATA CATTAGTTCG AGCCCTCATG ACAACTGTCT GTTATTCTGC AATTATCTTT
    GAGACTCCTC TCCGAGTGGA TGTTCAAGTG TTGAAAGTGC GAGCAAGACT GCTGCAGAAA
    TACCTGTGTG ATGAGCAGAA GGAGCTACAA GCACTCTATG CTCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG ATGTTCTTTG ATGCTCTATA TGATGAGGAC
    GTGGTGAAGG AAGACGCCTT CTACAGCTGG GAGAGCAGCA AGGATCCTGC TGAACAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCATTCTTCA ATTGGCTTCG TGAGGCTGAG
    GACGAGGAGT CTGATCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006522009.1
    CDS272..4795
    Translation

    Target ORF information:

    RefSeq Version XM_006521946.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006521946.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGATGATCC CTTCCCAGAT CTCCTACTCA GCCTCCCAAG GAGCCTACTA TATCCCTGGA 
    CAGGGGCGTT CCACATATGT TGTCCCAACA CAGCAGTACC CTGTGCAGCC AGGAGCCCCA
    GGCTTCTATC CAGGTGCAAG CCCTACCGAG TTTGGGACCT ATGCTGGAGC CTATTACCCA
    GCCCAAGGTG TGCAGCAGTT TCCTGCTAGT GTGGCTCCTG CCCCAGTTTT GATGAACCAG
    CCACCCCAGA TTGCTCCTAA GAGGGAACGG AAAACTATCC GAATTCGAGA CCCAAACCAA
    GGAGGGAAGG ATATCACAGA AGAGATCATG TCTGGGGCCC GCACTGCCTC CACACCCACT
    CCTCCCCAGA CGGGAGGCAG TCTGGAGCCT CAACCCAATG GGGAGTCGCC TCAGGTTGCT
    GTCATTATCC GGCCAGATGA CCGGTCGCAG GGAGCAGCCA TTGGGGGGCG GCCAGGACTG
    CCTGGCCCAG AGCATAGCCC TGGCACAGAA TCTCAGCCTT CGTCGCCTTC TCCAACCCCA
    TCACCACCCC CAATTTTGGA GCCGGGGTCT GAGTCTAATC TTGGAGTCCT CTCTATTCCT
    GGGGACACTA TGACAACAGG GATGATACCA ATGTCTGTAG AAGAATCGAC CCCTATCTCT
    TGTGAAACTG GGGAGCCGTA TTGCCTCTCT CCAGAACCCA CTCTTGCCGA ACCCATACTG
    GAAGTAGAAG TGACACTCAG CAAACCCATT CCAGAATCTG AGTTCTCTTC CAGTCCTCTC
    CAGGTTTCCA CGGCCCTGGT GCCTCACAAG GTTGAAACTC ATGAGCCCAA TGGCGTGATC
    CCATCTGAGG ATCTGGAACC AGAGGTGGAG TCAAGCACAG AGCCAGCTCC TCCCCCTCTA
    TCACCTTGTG CTTCTGAATC GCTCGTGCCC ATTGCTCCAA CTGCCCAGCC TGAGGAACTG
    CTCAACGGAG CCCCCTCACC ACCAGCTGTG GATTTAAGCC CAGTCAGTGA GCCAGAGGAA
    CAGGCCAAGA AGGTTTCATC AGCAGCACTG GCCAGCATTC TCTCTCCTGC TCCACCTGTG
    GCTCCTTCAG ATACTTCTCC TGCTCAGGAG GAAGAAATGG AAGAAGATGA CGATGACGAA
    GAAGGTGGAG AAGCTGAGAG TGAGAAGGGA GGAGAGGACG TCCCCCTTGA CAGTACTCCT
    GTCCCAGCCC AGCTGTCTCA GAATTTAGAG GTGGCAGCAG CTACCCAGGT GGCAGTGTCT
    GTGCCAAAGA GGAGACGGAA AATTAAAGAG CTAAATAAGA AGGAGGCTGT AGGAGACCTT
    CTAGATGCCT TCAAGGAGGT GGACCCAGCA GTACCAGAGG TAGAGAATCA GCCTCCCACT
    GGCAGCAACC CAAGCCCAGA GTCTGAGGGC AGCATGGTGC CCACACAGCC TGAGGAAACA
    GAAGAAACCT GGGACTCTAA GGAAGACAAA ATTCATAATG CTGAGAACAT CCAGCCTGGG
    GAGCAGAAGT ATGAGTACAA GTCAGATCAG TGGAAGCCTC TAAACCTTGA AGAAAAGAAG
    CGTTATGACA GAGAATTCCT GCTGGGCTTT CAGTTCATCT TTGCCAGCAT GCAGAAGCCA
    GAAGGATTGC CCCATATCAC TGATGTGGTG CTGGACAAGG CCAATAAAAC ACCACTTCGG
    CAACTGGATC CCTCCAGACT ACCTGGCATA AACTGTGGCC CAGATTTCAC TCCATCTTTT
    GCCAATCTTG GCCGACCAGC CCTCAGCAAC CGTGGGCCCC CGAGGGGTGG GCCAGGTGGG
    GAGCTGCCCC GAGGGCCGCA GGCTGGCCTG GGACCCAGGC GCTCTCAGCA GGGCCCACGA
    AAGGAAACTC GCAAGATCAT TTCCTCAGTG ATAATGACTG AAGACATAAA ACTAAATAAA
    GCAGAGAAGG CTTGGAAACC CAGTAGCAAA CGGACAGCAG CTGATAAGGA TCGAGGGGAA
    GAGGATGCTG ATGGAAGCAA GACCCAGGAC CTGTTCCGCA GGGTACGCTC CATCCTGAAT
    AAGCTGACAC CCCAGATGTT TCAGCAGCTG ATGAAGCAGG TGACACAGCT GGCCATTGAC
    ACGGAGGAAC GCCTCAAAGG AGTTATTGAC CTCATCTTTG AGAAAGCCAT TTCAGAGCCC
    AACTTCTCTG TGGCTTATGC CAACATGTGC CGCTGCCTCA TGGCGCTGAA AGTGCCCACT
    ACAGAAAAGC CAACAGTAAC TGTGAATTTT CGAAAACTGT TGTTAAATCG GTGCCAGAAG
    GAATTTGAGA AAGACAAAGA TGATGATGAA GTTTTTGAAA AAAAGCAAAA AGAGATGGAT
    GAAGCTGCTA CGGCAGAAGA ACGGGGACGC CTGAAAGAAG AGCTAGAAGA AGCCCGGGAC
    ATAGCTAGGC GGCGCTCTTT AGGGAATATC AAGTTTATTG GAGAGTTATT CAAGTTGAAG
    ATGTTAACAG AAGCAATAAT GCATGACTGT GTGGTCAAGC TACTTAAGAA CCATGATGAG
    GAGTCCCTGG AATGCCTCTG CCGCCTCCTC ACCACTATAG GCAAAGACCT GGACTTTGCA
    AAAGCCAAGC CTCGAATGGA TCAGTATTTC AACCAAATGG AAAAAATCAT TAAAGAAAAG
    AAGACCTCAT CTCGCATCCG TTTTATGCTG CAGGATGTAC TCGATCTGCG GCAGAGTAAT
    TGGGTGCCAC GCCGAGGGGA TCAGGGTCCT AAGACTATTG ATCAAATTCA CAAGGAGGCT
    GAGATGGAAG AGCACCGAGA ACATATTAAA GTACAGCAGC TCATGGCCAA GGGCAGCGAC
    AAACGTCGGG GTGGCCCTCC AGGCCCGCCT ATCAACCGTG GCCTTCCACT TGTAGATGAT
    GGTGGCTGGA ATACAGTCCC CATTAGCAAA GGCAGTCGCC CCATTGACAC CTCACGGCTC
    ACCAAGATCA CAAAGCCTGG TTCCATTGAT TCTAACAACC AGCTTTTTGC ACCTGGAGGA
    CGACTGAGTT GGGGCAAGGG CAGCAGTGGG GGCTCAGGAG CCAAGCCCTC AGACACAGCA
    TCGGAAGCTA CTCGTCCAGC TACCTTGAAT CGCTTTTCTG CTCTTCAACA AACATTACCC
    GCAGAGAACA CAGATAACAG ACGTGTTGTA CAGAGGAGTA GCTTGAGCCG GGAACGAGGT
    GAGAAAGCTG GGGACCGGGG AGACCGACTA GAGCGGAGTG AGCGGGGAGG TGACCGTGGG
    GACCGACTTG ATCGTGCCAG AACACCTGCC ACCAAGCGAA GTTTTAGCAA AGAAGTGGAG
    GAGCGAAGTA GAGAGCGGCC ATCCCAGCCT GAGGGACTCC GCAAGGCAGC TAGCCTCACA
    GAGGATCGTG GTCGGGATCC TGTGAAGCGG GAAGCCACTC TACCTCCAGT GAGCCCTCCA
    AAGGCTGCGC TGTCTGTGGA TGAGGTGGAG AAGAAATCTA AGGCCATCAT TGAGGAATAT
    CTCCATCTCA ATGACATGAA GGAGGCAGTA CAGTGTGTCC AGGAGCTGGC TTCACCCTCC
    TTGCTCTTCA TCTTCGTACG GCTTGGTATC GAGTCCACGC TGGAGCGTAG TACCATTGCT
    CGTGAGCATA TGGGGCGACT ACTGCACCAA CTGCTCTGTG CGGGGCACCT CTCTACTGCC
    CAGTACTATC AAGGGCTGTA TGAAACACTA GAATTGGCTG AGGACATGGA AATTGATATC
    CCTCATGTAT GGCTTTACCT GGCAGAACTG ATTACACCTA TTCTCCAGGA AGATGGGGTG
    CCCATGGGAG AGCTCTTCAG GGAAATTACG AAGCCTCTGA GACCCATGGG CAAAGCTACT
    TCTTTATTGC TGGAGATCCT GGGTCTCTTA TGCAAGAGCA TGGGTCCCAA AAAGGTGGGG
    ATGCTGTGGC GAGAGGCTGG GCTGAGCTGG AGGGAATTTC TAGCAGAGGG CCAAGACGTT
    GGCTCATTTG TGGCTGAAAA GAAGGTGGAA TATACCTTGG GAGAAGAATC TGAAGCTCCT
    GGCCAGAGGA CACTTGCCTT TGAGGAGCTT CGTAGGCAGC TAGAGAAGCT GCTGAAGGAC
    GGCGGCAGTA ATCAGCGTGT GTTCGACTGG ATAGACGCCA ACCTAAATGA GCAGCAGATA
    GCATCCAATA CATTAGTTCG AGCCCTCATG ACAACTGTCT GTTATTCTGC AATTATCTTT
    GAGACTCCTC TCCGAGTGGA TGTTCAAGTG TTGAAAGTGC GAGCAAGACT GCTGCAGAAA
    TACCTGTGTG ATGAGCAGAA GGAGCTACAA GCACTCTATG CTCTCCAGGC CCTTGTAGTG
    ACCTTAGAAC AGCCTGCCAA CCTGCTTCGG ATGTTCTTTG ATGCTCTATA TGATGAGGAC
    GTGGTGAAGG AAGACGCCTT CTACAGCTGG GAGAGCAGCA AGGATCCTGC TGAACAGCAG
    GGCAAGGGTG TGGCCCTTAA ATCTGTCACA GCATTCTTCA ATTGGCTTCG TGAGGCTGAG
    GACGAGGAGT CTGATCACAA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72646
    Clone ID Related Accession (Same CDS sequence) XM_011245861.1
    Accession Version XM_011245861.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4776bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product eukaryotic translation initiation factor 4 gamma 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000082.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG
    CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA
    GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT
    TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA
    GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC
    TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG
    CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG
    CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT
    TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC
    CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG
    ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC
    GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT
    TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC
    AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT
    CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG
    CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT
    GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT
    GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT
    GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT
    GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG
    GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT
    GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT
    CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG
    CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC
    ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT
    GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC
    ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA
    ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC
    ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT
    GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG
    CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA
    AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG
    GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC
    TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG
    CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC
    ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG
    AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT
    CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA
    AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA
    GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA
    TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG
    AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC
    CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC
    ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG
    CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT
    CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC
    AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA
    CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC
    ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT
    GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC
    TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA
    CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC
    CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA
    GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC
    AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA
    GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA
    GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC
    ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG
    GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT
    AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC
    CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG
    GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG
    GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG
    GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC
    AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG
    GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA
    TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG
    CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT
    GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT
    GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA
    CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG
    GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA
    TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT
    GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT
    CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011244163.1
    CDS249..5024
    Translation

    Target ORF information:

    RefSeq Version XM_011245861.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus eukaryotic translation initiation factor 4, gamma 1 (Eif4g1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011245861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAACAAAG CTCCCCAGCC CACAGGCCCC CCACCCGCCA GGTCCCCTGG ACTCCCACAG 
    CCAGCGTTTC CCCCGGGGCA GACTGCACCG GTGGTGTTTA GCACGCCTCA AGCGACACAA
    ATGAACACGC CTTCTCAGCC CCGCCAGGGA GGATTCAGGT CTCTGCAGCA CTTCTACCCT
    AGCCGGGCCC AGCCCCCTAG CAGTGCAGCC TCCCGAGTGC AGAGTGCAGC CCCTGCCCGT
    CCTGGCCCAG CTCCCCATGT CTACCCTGCT GGATCCCAAG TAATGATGAT CCCTTCCCAG
    ATCTCCTACT CAGCCTCCCA AGGAGCCTAC TATATCCCTG GACAGCAGTA CCCTGTGCAG
    CCAGGAGCCC CAGGCTTCTA TCCAGGTGCA AGCCCTACCG AGTTTGGGAC CTATGCTGGA
    GCCTATTACC CAGCCCAAGG TGTGCAGCAG TTTCCTGCTA GTGTGGCTCC TGCCCCAGTT
    TTGATGAACC AGCCACCCCA GATTGCTCCT AAGAGGGAAC GGAAAACTAT CCGAATTCGA
    GACCCAAACC AAGGAGGGAA GGATATCACA GAAGAGATCA TGTCTGGGGC CCGCACTGCC
    TCCACACCCA CTCCTCCCCA GACGGGAGGC AGTCTGGAGC CTCAACCCAA TGGGGAGTCG
    CCTCAGGTTG CTGTCATTAT CCGGCCAGAT GACCGGTCGC AGGGAGCAGC CATTGGGGGG
    CGGCCAGGAC TGCCTGGCCC AGAGCATAGC CCTGGCACAG AATCTCAGCC TTCGTCGCCT
    TCTCCAACCC CATCACCACC CCCAATTTTG GAGCCGGGGT CTGAGTCTAA TCTTGGAGTC
    CTCTCTATTC CTGGGGACAC TATGACAACA GGGATGATAC CAATGTCTGT AGAAGAATCG
    ACCCCTATCT CTTGTGAAAC TGGGGAGCCG TATTGCCTCT CTCCAGAACC CACTCTTGCC
    GAACCCATAC TGGAAGTAGA AGTGACACTC AGCAAACCCA TTCCAGAATC TGAGTTCTCT
    TCCAGTCCTC TCCAGGTTTC CACGGCCCTG GTGCCTCACA AGGTTGAAAC TCATGAGCCC
    AATGGCGTGA TCCCATCTGA GGATCTGGAA CCAGAGGTGG AGTCAAGCAC AGAGCCAGCT
    CCTCCCCCTC TATCACCTTG TGCTTCTGAA TCGCTCGTGC CCATTGCTCC AACTGCCCAG
    CCTGAGGAAC TGCTCAACGG AGCCCCCTCA CCACCAGCTG TGGATTTAAG CCCAGTCAGT
    GAGCCAGAGG AACAGGCCAA GAAGGTTTCA TCAGCAGCAC TGGCCAGCAT TCTCTCTCCT
    GCTCCACCTG TGGCTCCTTC AGATACTTCT CCTGCTCAGG AGGAAGAAAT GGAAGAAGAT
    GACGATGACG AAGAAGGTGG AGAAGCTGAG AGTGAGAAGG GAGGAGAGGA CGTCCCCCTT
    GACAGTACTC CTGTCCCAGC CCAGCTGTCT CAGAATTTAG AGGTGGCAGC AGCTACCCAG
    GTGGCAGTGT CTGTGCCAAA GAGGAGACGG AAAATTAAAG AGCTAAATAA GAAGGAGGCT
    GTAGGAGACC TTCTAGATGC CTTCAAGGAG GTGGACCCAG CAGTACCAGA GGTAGAGAAT
    CAGCCTCCCA CTGGCAGCAA CCCAAGCCCA GAGTCTGAGG GCAGCATGGT GCCCACACAG
    CCTGAGGAAA CAGAAGAAAC CTGGGACTCT AAGGAAGACA AAATTCATAA TGCTGAGAAC
    ATCCAGCCTG GGGAGCAGAA GTATGAGTAC AAGTCAGATC AGTGGAAGCC TCTAAACCTT
    GAAGAAAAGA AGCGTTATGA CAGAGAATTC CTGCTGGGCT TTCAGTTCAT CTTTGCCAGC
    ATGCAGAAGC CAGAAGGATT GCCCCATATC ACTGATGTGG TGCTGGACAA GGCCAATAAA
    ACACCACTTC GGCAACTGGA TCCCTCCAGA CTACCTGGCA TAAACTGTGG CCCAGATTTC
    ACTCCATCTT TTGCCAATCT TGGCCGACCA GCCCTCAGCA ACCGTGGGCC CCCGAGGGGT
    GGGCCAGGTG GGGAGCTGCC CCGAGGGCCG CAGGCTGGCC TGGGACCCAG GCGCTCTCAG
    CAGGGCCCAC GAAAGGAAAC TCGCAAGATC ATTTCCTCAG TGATAATGAC TGAAGACATA
    AAACTAAATA AAGCAGAGAA GGCTTGGAAA CCCAGTAGCA AACGGACAGC AGCTGATAAG
    GATCGAGGGG AAGAGGATGC TGATGGAAGC AAGACCCAGG ACCTGTTCCG CAGGGTACGC
    TCCATCCTGA ATAAGCTGAC ACCCCAGATG TTTCAGCAGC TGATGAAGCA GGTGACACAG
    CTGGCCATTG ACACGGAGGA ACGCCTCAAA GGAGTTATTG ACCTCATCTT TGAGAAAGCC
    ATTTCAGAGC CCAACTTCTC TGTGGCTTAT GCCAACATGT GCCGCTGCCT CATGGCGCTG
    AAAGTGCCCA CTACAGAAAA GCCAACAGTA ACTGTGAATT TTCGAAAACT GTTGTTAAAT
    CGGTGCCAGA AGGAATTTGA GAAAGACAAA GATGATGATG AAGTTTTTGA AAAAAAGCAA
    AAAGAGATGG ATGAAGCTGC TACGGCAGAA GAACGGGGAC GCCTGAAAGA AGAGCTAGAA
    GAAGCCCGGG ACATAGCTAG GCGGCGCTCT TTAGGGAATA TCAAGTTTAT TGGAGAGTTA
    TTCAAGTTGA AGATGTTAAC AGAAGCAATA ATGCATGACT GTGTGGTCAA GCTACTTAAG
    AACCATGATG AGGAGTCCCT GGAATGCCTC TGCCGCCTCC TCACCACTAT AGGCAAAGAC
    CTGGACTTTG CAAAAGCCAA GCCTCGAATG GATCAGTATT TCAACCAAAT GGAAAAAATC
    ATTAAAGAAA AGAAGACCTC ATCTCGCATC CGTTTTATGC TGCAGGATGT ACTCGATCTG
    CGGCAGAGTA ATTGGGTGCC ACGCCGAGGG GATCAGGGTC CTAAGACTAT TGATCAAATT
    CACAAGGAGG CTGAGATGGA AGAGCACCGA GAACATATTA AAGTACAGCA GCTCATGGCC
    AAGGGCAGCG ACAAACGTCG GGGTGGCCCT CCAGGCCCGC CTATCAACCG TGGCCTTCCA
    CTTGTAGATG ATGGTGGCTG GAATACAGTC CCCATTAGCA AAGGCAGTCG CCCCATTGAC
    ACCTCACGGC TCACCAAGAT CACAAAGCCT GGTTCCATTG ATTCTAACAA CCAGCTTTTT
    GCACCTGGAG GACGACTGAG TTGGGGCAAG GGCAGCAGTG GGGGCTCAGG AGCCAAGCCC
    TCAGACACAG CATCGGAAGC TACTCGTCCA GCTACCTTGA ATCGCTTTTC TGCTCTTCAA
    CAAACATTAC CCGCAGAGAA CACAGATAAC AGACGTGTTG TACAGAGGAG TAGCTTGAGC
    CGGGAACGAG GTGAGAAAGC TGGGGACCGG GGAGACCGAC TAGAGCGGAG TGAGCGGGGA
    GGTGACCGTG GGGACCGACT TGATCGTGCC AGAACACCTG CCACCAAGCG AAGTTTTAGC
    AAAGAAGTGG AGGAGCGAAG TAGAGAGCGG CCATCCCAGC CTGAGGGACT CCGCAAGGCA
    GCTAGCCTCA CAGAGGATCG TGGTCGGGAT CCTGTGAAGC GGGAAGCCAC TCTACCTCCA
    GTGAGCCCTC CAAAGGCTGC GCTGTCTGTG GATGAGGTGG AGAAGAAATC TAAGGCCATC
    ATTGAGGAAT ATCTCCATCT CAATGACATG AAGGAGGCAG TACAGTGTGT CCAGGAGCTG
    GCTTCACCCT CCTTGCTCTT CATCTTCGTA CGGCTTGGTA TCGAGTCCAC GCTGGAGCGT
    AGTACCATTG CTCGTGAGCA TATGGGGCGA CTACTGCACC AACTGCTCTG TGCGGGGCAC
    CTCTCTACTG CCCAGTACTA TCAAGGGCTG TATGAAACAC TAGAATTGGC TGAGGACATG
    GAAATTGATA TCCCTCATGT ATGGCTTTAC CTGGCAGAAC TGATTACACC TATTCTCCAG
    GAAGATGGGG TGCCCATGGG AGAGCTCTTC AGGGAAATTA CGAAGCCTCT GAGACCCATG
    GGCAAAGCTA CTTCTTTATT GCTGGAGATC CTGGGTCTCT TATGCAAGAG CATGGGTCCC
    AAAAAGGTGG GGATGCTGTG GCGAGAGGCT GGGCTGAGCT GGAGGGAATT TCTAGCAGAG
    GGCCAAGACG TTGGCTCATT TGTGGCTGAA AAGAAGGTGG AATATACCTT GGGAGAAGAA
    TCTGAAGCTC CTGGCCAGAG GACACTTGCC TTTGAGGAGC TTCGTAGGCA GCTAGAGAAG
    CTGCTGAAGG ACGGCGGCAG TAATCAGCGT GTGTTCGACT GGATAGACGC CAACCTAAAT
    GAGCAGCAGA TAGCATCCAA TACATTAGTT CGAGCCCTCA TGACAACTGT CTGTTATTCT
    GCAATTATCT TTGAGACTCC TCTCCGAGTG GATGTTCAAG TGTTGAAAGT GCGAGCAAGA
    CTGCTGCAGA AATACCTGTG TGATGAGCAG AAGGAGCTAC AAGCACTCTA TGCTCTCCAG
    GCCCTTGTAG TGACCTTAGA ACAGCCTGCC AACCTGCTTC GGATGTTCTT TGATGCTCTA
    TATGATGAGG ACGTGGTGAA GGAAGACGCC TTCTACAGCT GGGAGAGCAG CAAGGATCCT
    GCTGAACAGC AGGGCAAGGG TGTGGCCCTT AAATCTGTCA CAGCATTCTT CAATTGGCTT
    CGTGAGGCTG AGGACGAGGA GTCTGATCAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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