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Eif2a cDNA ORF clone, Mus musculus(house mouse)

Summary

Gene Symbol Eif2a
Entrez Gene ID 229317
Full Name eukaryotic translation initiation factor 2A
Synonyms D030048D22,D3Ertd194e
General protein information
Preferred Names

eukaryotic translation initiation factor 2A

Names

eukaryotic translation initiation factor 2A
eIF-2A
Gene Type protein-coding
Organism Mus musculus(house mouse)
Genome

3

3 D|3 28.58 cM

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_006501321.3 XP_006501384.1 eukaryotic translation initiation factor 2A isoform X1
NM_001005509.2 NP_001005509.1 eukaryotic translation initiation factor 2A
XM_006501321.4 XP_006501384.1 eukaryotic translation initiation factor 2A isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) EIF2A NP_114414.2
Pan troglodytes (chimpanzee) EIF2A XP_001143578.1
Canis lupus familiaris (dog) EIF2A XP_534306.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP002525 XP_312412.4
Caenorhabditis elegans (roundworm) E04D5.1 NP_496239.2
Schizosaccharomyces pombe (fission yeast) SPBC4B4.04 NP_596421.1
Bos taurus (cattle) EIF2A NP_001095753.1
Drosophila melanogaster (fruit fly) CG7414 NP_649365.1
EIF2A XP_001108004.2
Mus musculus (house mouse) Eif2a NP_001005509.1
Saccharomyces cerevisiae (baker's yeast) YGR054W NP_011568.3
Eremothecium gossypii AGOS_AEL286C NP_984574.2
Arabidopsis thaliana (thale cress) AT1G73180 NP_565058.1
Kluyveromyces lactis KLLA0A02761g XP_451122.1
Magnaporthe oryzae (rice blast fungus) MGG_00847 XP_003718132.1
Neurospora crassa NCU06099 XP_959961.1
Xenopus tropicalis (tropical clawed frog) eif2a NP_001120561.1
Rattus norvegicus (Norway rat) Eif2a NP_001102809.1
Gallus gallus (chicken) EIF2A NP_001026494.1
Danio rerio (zebrafish) eif2a NP_001020341.1

Gene Ontology


Bibliography

Related articles in PubMed

HRD1 prevents apoptosis in renal tubular epithelial cells by mediating eIF2? ubiquitylation and degradation.
Huang Y, Sun Y, Cao Y, Sun H, Li M, You H, Su D, Li Y, Liang X
Cell death & disease8(12)3202(2017 Dec)

Neuroprotection by eIF2?-CHOP inhibition and XBP-1 activation in EAE/optic neuritiss.
Huang H, Miao L, Liang F, Liu X, Xu L, Teng X, Wang Q, Ridder WH 3rd, Shindler KS, Sun Y, Hu Y
Cell death & disease8(7)e2936(2017 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results further extended the understanding of Heme-regulated eIF2alpha kinase on the regulation of non-erythroid lineages and might provide new aspects for preventing and treating Cd-induced detrimental effects
Title: A protective role of Heme-regulated eIF2? kinase in cadmium-induced liver and kidney injuries.

Data, including data from studies using cells from knockout mice, suggest that gasotransmitter H(2)S up-regulates eIF2a phosphorylation by inhibiting PPP1CA via persulfidation, which in turn leads to transient suppression of global translation and activation of Atf4 expression. (eIF2a = eukaryotic initiation factor-2alpha; PPP1CA = protein phosphatase 1 catalytic subunit alpha; Atf4 = activating transcription factor 4)
Title: Hydrogen sulfide modulates eukaryotic translation initiation factor 2? (eIF2?) phosphorylation status in the integrated stress-response pathway.

stress granule formation is a downstream event of eIF2alpha phosphorylation that may not directly protect cells from apoptosis, at least in spermatocytes of seminiferous tubules in early stages.
Title: Importance of eIF2? phosphorylation as a protective mechanism against heat stress in mouse male germ cells.

Results mechanistically link multiple forms of dystonia and put forth a new overall cellular mechanism for dystonia pathogenesis, impairment of eIF2alpha signaling, a pathway known for its roles in cellular stress responses and synaptic plasticity.
Title: Functional Genomic Analyses of Mendelian and Sporadic Disease Identify Impaired eIF2? Signaling as a Generalizable Mechanism for Dystonia.

The eIF2A knock out mice strain is viable and to date displays no apparent phenotype.
Title: The eIF2A knockout mouse.

The following Eif2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Eif2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu18130 NM_001005509.2
Latest version!		 Mus musculus eukaryotic translation initiation factor 2A (Eif2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $342.30
$489.00
OMu31804 XM_006501321.4
Latest version!		 Mus musculus eukaryotic translation initiation factor 2A (Eif2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OMu31804 XM_006501321.3 Mus musculus eukaryotic translation initiation factor 2A (Eif2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OMu31804
Clone ID Related Accession (Same CDS sequence) XM_006501321.3 , XM_006501321.4
Accession Version XM_006501321.3 Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1569bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-06-21
Organism Mus musculus(house mouse)
Product eukaryotic translation initiation factor 2A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006501321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGACCACC ACACTTCACA GAAAGCACGG TGCTTCCCAG AGAATCTGGA AGAAATTGCA 
AAGTGTACAC TTTTAGTAAG GATGGGACAT TGTTTGCCTG GAGCAATGGA GAAAACTTCT 
AAAGATGGCA CTGCTGGGAC GCCTAACCTA CAACTTTATG ACATGAAAAC TGGAGCATGT 
TTGAAATCGT TCATCCAGAA AAAAATGCAA AATTGGTGTC CTTCCTGGTC AGATGATGAA 
ATTATTTGTG CCCGGAATGT TAACAACGAA GTTCACTTCT TTGAGAACAA CAATTTTAAC 
ACAATTGCAA ATAAATTACA TTTGCAAAAA GTTAATGACT TTAATTTATC ACCTGGAACC 
CAACCTTATA AGGTGGCAGT TTATGTCCCA GGAAGTAAAG GTGCCCCCTC ATTTGTTCGA 
CTGTATCAGT ATCCCAACTT TGCCGGACCT CAGGCAGCAC TGGCCAATAA AAGCTTCTTT 
AAAGCTGATA AGGTTACAAT GCTGTGGAAT AAAAAAGCTA CTGCTGTGCT GGTAATAGCC 
AGTACAGAGG TTGACAAGAC AGGAGCCTCC TACTATGGAG AACAAACACT GCACTACATT 
GCAACAAATG GAGAAAGTGC TGTGGTACAA TTACCAAAAA ATGGCCCAAT TTATGATGTA 
GTTTGGAATT CCAGTTCTAC TGAGTTTTGC GCTGTTTATG GTTTTATGCC TGCGAAAGCA 
ACAGTTTTCA ACCTGAAGTG TGATCCTGTG TTTGACTTTG GGACTGGTCC TCGCAACGCA 
GCCTTCTATA GCCCTCATGG ACATATATTA GTGCTGGCTG GATTTGGAAA TCTTCGAGGA 
CAAATGGAAG TATGGGATGT TAAAAACTAC AAGCTTATTT CTAAGCCAGT GGCTTCTGAT 
TCTACATATT TTGCCTGGTG CCCAGATGGT GAGCATATCT TAACAGCCAC ATGTGCTCCT 
AGGTTACGTG TTAATAATGG GTATAAGATC TGGCATTATA CTGGCTCTCT TTTGCATAAG 
TATGATGTGC CATCAAATGG AGAATTATGG CAGGTTTCTT GGCAGCCATT TTTGGATGGA 
ATATTTCCAG CAAAAACGAT AAAGTACCAA GCAGTTCCAA GTGAAGTGCC TAGTGAGGAG 
CCTAAAGTTG CAACAGCTTA TAGACCTCCA GCCTTAAGAA ATAAGCCAGT CACCAATTCC 
AAACTGCATG AAGAGGAACC TCCCCAGAAT ATGAAGCCAC ATCCAGGAAG TGACAAGCCA 
TTATCAAAAA CAGCCCTTAA AAATCAAAGG AAGCATGAAG CTAAAAAAGC TGCAAAACAG 
GAAGCAAGAA GTGATGCGGC TCCTACTCCT GTCCCACAGA GTGCACCACG GAACACGGTC 
ACCCAGTCTG CTTCTGGTGA TCCTGAAGTA GACAAAAAAA TTAAGAATCT AAAGAAGAAA 
CTGAAAGCCA TAGAGCAGCT AAAAGAGCAG GCAGCCGCCG GGAAACAGCT AGAAAAAAAT 
CAGTTGGAGA AAATTCAGAA AGAGACAGCC CTTCTCCAAG AGCTTGAAGA TTTGGAATTG 
GGAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006501384.1
CDS207..1775
Translation

Target ORF information:

RefSeq Version XM_006501321.3
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2A (Eif2a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006501321.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGACCACC ACACTTCACA GAAAGCACGG TGCTTCCCAG AGAATCTGGA AGAAATTGCA 
AAGTGTACAC TTTTAGTAAG GATGGGACAT TGTTTGCCTG GAGCAATGGA GAAAACTTCT 
AAAGATGGCA CTGCTGGGAC GCCTAACCTA CAACTTTATG ACATGAAAAC TGGAGCATGT 
TTGAAATCGT TCATCCAGAA AAAAATGCAA AATTGGTGTC CTTCCTGGTC AGATGATGAA 
ATTATTTGTG CCCGGAATGT TAACAACGAA GTTCACTTCT TTGAGAACAA CAATTTTAAC 
ACAATTGCAA ATAAATTACA TTTGCAAAAA GTTAATGACT TTAATTTATC ACCTGGAACC 
CAACCTTATA AGGTGGCAGT TTATGTCCCA GGAAGTAAAG GTGCCCCCTC ATTTGTTCGA 
CTGTATCAGT ATCCCAACTT TGCCGGACCT CAGGCAGCAC TGGCCAATAA AAGCTTCTTT 
AAAGCTGATA AGGTTACAAT GCTGTGGAAT AAAAAAGCTA CTGCTGTGCT GGTAATAGCC 
AGTACAGAGG TTGACAAGAC AGGAGCCTCC TACTATGGAG AACAAACACT GCACTACATT 
GCAACAAATG GAGAAAGTGC TGTGGTACAA TTACCAAAAA ATGGCCCAAT TTATGATGTA 
GTTTGGAATT CCAGTTCTAC TGAGTTTTGC GCTGTTTATG GTTTTATGCC TGCGAAAGCA 
ACAGTTTTCA ACCTGAAGTG TGATCCTGTG TTTGACTTTG GGACTGGTCC TCGCAACGCA 
GCCTTCTATA GCCCTCATGG ACATATATTA GTGCTGGCTG GATTTGGAAA TCTTCGAGGA 
CAAATGGAAG TATGGGATGT TAAAAACTAC AAGCTTATTT CTAAGCCAGT GGCTTCTGAT 
TCTACATATT TTGCCTGGTG CCCAGATGGT GAGCATATCT TAACAGCCAC ATGTGCTCCT 
AGGTTACGTG TTAATAATGG GTATAAGATC TGGCATTATA CTGGCTCTCT TTTGCATAAG 
TATGATGTGC CATCAAATGG AGAATTATGG CAGGTTTCTT GGCAGCCATT TTTGGATGGA 
ATATTTCCAG CAAAAACGAT AAAGTACCAA GCAGTTCCAA GTGAAGTGCC TAGTGAGGAG 
CCTAAAGTTG CAACAGCTTA TAGACCTCCA GCCTTAAGAA ATAAGCCAGT CACCAATTCC 
AAACTGCATG AAGAGGAACC TCCCCAGAAT ATGAAGCCAC ATCCAGGAAG TGACAAGCCA 
TTATCAAAAA CAGCCCTTAA AAATCAAAGG AAGCATGAAG CTAAAAAAGC TGCAAAACAG 
GAAGCAAGAA GTGATGCGGC TCCTACTCCT GTCCCACAGA GTGCACCACG GAACACGGTC 
ACCCAGTCTG CTTCTGGTGA TCCTGAAGTA GACAAAAAAA TTAAGAATCT AAAGAAGAAA 
CTGAAAGCCA TAGAGCAGCT AAAAGAGCAG GCAGCCGCCG GGAAACAGCT AGAAAAAAAT 
CAGTTGGAGA AAATTCAGAA AGAGACAGCC CTTCTCCAAG AGCTTGAAGA TTTGGAATTG 
GGAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu18130
Clone ID Related Accession (Same CDS sequence) NM_001005509.2
Accession Version NM_001005509.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1746bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-27
Organism Mus musculus(house mouse)
Product eukaryotic translation initiation factor 2A
Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY035972.1 and BC082557.1. On Jun 20, 2009 this sequence version replaced NM_001005509.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC082557.1, AK145275.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGCCGT CCACACCGCT GTTGACAGTC AGAGGATCAG AGGGACTGTA CATGGTGAAT 
GGACCACCAC ACTTCACAGA AAGCACGGTG CTTCCCAGAG AATCTGGAAG AAATTGCAAA 
GTGTACACTT TTAGTAAGGA TGGGACATTG TTTGCCTGGA GCAATGGAGA AAAAGTAAAT 
ATAATCAATG TCGCTAACAA GGGACTACTG CACTCCTTCG ACCTCCCAAA AGCAGTTTGC 
CTTGAATTCT CACCAAATAA CACTGTCCTG GCAACGTGGC AGCCTTACAC TACTTCTAAA 
GATGGCACTG CTGGGACGCC TAACCTACAA CTTTATGACA TGAAAACTGG AGCATGTTTG 
AAATCGTTCA TCCAGAAAAA AATGCAAAAT TGGTGTCCTT CCTGGTCAGA TGATGAAATT 
ATTTGTGCCC GGAATGTTAA CAACGAAGTT CACTTCTTTG AGAACAACAA TTTTAACACA 
ATTGCAAATA AATTACATTT GCAAAAAGTT AATGACTTTA ATTTATCACC TGGAACCCAA 
CCTTATAAGG TGGCAGTTTA TGTCCCAGGA AGTAAAGGTG CCCCCTCATT TGTTCGACTG 
TATCAGTATC CCAACTTTGC CGGACCTCAG GCAGCACTGG CCAATAAAAG CTTCTTTAAA 
GCTGATAAGG TTACAATGCT GTGGAATAAA AAAGCTACTG CTGTGCTGGT AATAGCCAGT 
ACAGAGGTTG ACAAGACAGG AGCCTCCTAC TATGGAGAAC AAACACTGCA CTACATTGCA 
ACAAATGGAG AAAGTGCTGT GGTACAATTA CCAAAAAATG GCCCAATTTA TGATGTAGTT 
TGGAATTCCA GTTCTACTGA GTTTTGCGCT GTTTATGGTT TTATGCCTGC GAAAGCAACA 
GTTTTCAACC TGAAGTGTGA TCCTGTGTTT GACTTTGGGA CTGGTCCTCG CAACGCAGCC 
TTCTATAGCC CTCATGGACA TATATTAGTG CTGGCTGGAT TTGGAAATCT TCGAGGACAA 
ATGGAAGTAT GGGATGTTAA AAACTACAAG CTTATTTCTA AGCCAGTGGC TTCTGATTCT 
ACATATTTTG CCTGGTGCCC AGATGGTGAG CATATCTTAA CAGCCACATG TGCTCCTAGG 
TTACGTGTTA ATAATGGGTA TAAGATCTGG CATTATACTG GCTCTCTTTT GCATAAGTAT 
GATGTGCCAT CAAATGGAGA ATTATGGCAG GTTTCTTGGC AGCCATTTTT GGATGGAATA 
TTTCCAGCAA AAACGATAAA GTACCAAGCA GTTCCAAGTG AAGTGCCTAG TGAGGAGCCT 
AAAGTTGCAA CAGCTTATAG ACCTCCAGCC TTAAGAAATA AGCCAGTCAC CAATTCCAAA 
CTGCATGAAG AGGAACCTCC CCAGAATATG AAGCCACATC CAGGAAGTGA CAAGCCATTA 
TCAAAAACAG CCCTTAAAAA TCAAAGGAAG CATGAAGCTA AAAAAGCTGC AAAACAGGAA 
GCAAGAAGTG ATGCGGCTCC TACTCCTGTC CCACAGAGTG CACCACGGAA CACGGTCACC 
CAGTCTGCTT CTGGTGATCC TGAAGTAGAC AAAAAAATTA AGAATCTAAA GAAGAAACTG 
AAAGCCATAG AGCAGCTAAA AGAGCAGGCA GCCGCCGGGA AACAGCTAGA AAAAAATCAG 
TTGGAGAAAA TTCAGAAAGA GACAGCCCTT CTCCAAGAGC TTGAAGATTT GGAATTGGGA 
GTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001005509.1
CDS239..1984
Translation

Target ORF information:

RefSeq Version NM_001005509.2
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2A (Eif2a), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001005509.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGCGCCGT CCACACCGCT GTTGACAGTC AGAGGATCAG AGGGACTGTA CATGGTGAAT 
GGACCACCAC ACTTCACAGA AAGCACGGTG CTTCCCAGAG AATCTGGAAG AAATTGCAAA 
GTGTACACTT TTAGTAAGGA TGGGACATTG TTTGCCTGGA GCAATGGAGA AAAAGTAAAT 
ATAATCAATG TCGCTAACAA GGGACTACTG CACTCCTTCG ACCTCCCAAA AGCAGTTTGC 
CTTGAATTCT CACCAAATAA CACTGTCCTG GCAACGTGGC AGCCTTACAC TACTTCTAAA 
GATGGCACTG CTGGGACGCC TAACCTACAA CTTTATGACA TGAAAACTGG AGCATGTTTG 
AAATCGTTCA TCCAGAAAAA AATGCAAAAT TGGTGTCCTT CCTGGTCAGA TGATGAAATT 
ATTTGTGCCC GGAATGTTAA CAACGAAGTT CACTTCTTTG AGAACAACAA TTTTAACACA 
ATTGCAAATA AATTACATTT GCAAAAAGTT AATGACTTTA ATTTATCACC TGGAACCCAA 
CCTTATAAGG TGGCAGTTTA TGTCCCAGGA AGTAAAGGTG CCCCCTCATT TGTTCGACTG 
TATCAGTATC CCAACTTTGC CGGACCTCAG GCAGCACTGG CCAATAAAAG CTTCTTTAAA 
GCTGATAAGG TTACAATGCT GTGGAATAAA AAAGCTACTG CTGTGCTGGT AATAGCCAGT 
ACAGAGGTTG ACAAGACAGG AGCCTCCTAC TATGGAGAAC AAACACTGCA CTACATTGCA 
ACAAATGGAG AAAGTGCTGT GGTACAATTA CCAAAAAATG GCCCAATTTA TGATGTAGTT 
TGGAATTCCA GTTCTACTGA GTTTTGCGCT GTTTATGGTT TTATGCCTGC GAAAGCAACA 
GTTTTCAACC TGAAGTGTGA TCCTGTGTTT GACTTTGGGA CTGGTCCTCG CAACGCAGCC 
TTCTATAGCC CTCATGGACA TATATTAGTG CTGGCTGGAT TTGGAAATCT TCGAGGACAA 
ATGGAAGTAT GGGATGTTAA AAACTACAAG CTTATTTCTA AGCCAGTGGC TTCTGATTCT 
ACATATTTTG CCTGGTGCCC AGATGGTGAG CATATCTTAA CAGCCACATG TGCTCCTAGG 
TTACGTGTTA ATAATGGGTA TAAGATCTGG CATTATACTG GCTCTCTTTT GCATAAGTAT 
GATGTGCCAT CAAATGGAGA ATTATGGCAG GTTTCTTGGC AGCCATTTTT GGATGGAATA 
TTTCCAGCAA AAACGATAAA GTACCAAGCA GTTCCAAGTG AAGTGCCTAG TGAGGAGCCT 
AAAGTTGCAA CAGCTTATAG ACCTCCAGCC TTAAGAAATA AGCCAGTCAC CAATTCCAAA 
CTGCATGAAG AGGAACCTCC CCAGAATATG AAGCCACATC CAGGAAGTGA CAAGCCATTA 
TCAAAAACAG CCCTTAAAAA TCAAAGGAAG CATGAAGCTA AAAAAGCTGC AAAACAGGAA 
GCAAGAAGTG ATGCGGCTCC TACTCCTGTC CCACAGAGTG CACCACGGAA CACGGTCACC 
CAGTCTGCTT CTGGTGATCC TGAAGTAGAC AAAAAAATTA AGAATCTAAA GAAGAAACTG 
AAAGCCATAG AGCAGCTAAA AGAGCAGGCA GCCGCCGGGA AACAGCTAGA AAAAAATCAG 
TTGGAGAAAA TTCAGAAAGA GACAGCCCTT CTCCAAGAGC TTGAAGATTT GGAATTGGGA 
GTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OMu31804
Clone ID Related Accession (Same CDS sequence) XM_006501321.3 , XM_006501321.4
Accession Version XM_006501321.4 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1569bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-08-07
Organism Mus musculus(house mouse)
Product eukaryotic translation initiation factor 2A isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000069.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006501321.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGACCACC ACACTTCACA GAAAGCACGG TGCTTCCCAG AGAATCTGGA AGAAATTGCA 
AAGTGTACAC TTTTAGTAAG GATGGGACAT TGTTTGCCTG GAGCAATGGA GAAAACTTCT 
AAAGATGGCA CTGCTGGGAC GCCTAACCTA CAACTTTATG ACATGAAAAC TGGAGCATGT 
TTGAAATCGT TCATCCAGAA AAAAATGCAA AATTGGTGTC CTTCCTGGTC AGATGATGAA 
ATTATTTGTG CCCGGAATGT TAACAACGAA GTTCACTTCT TTGAGAACAA CAATTTTAAC 
ACAATTGCAA ATAAATTACA TTTGCAAAAA GTTAATGACT TTAATTTATC ACCTGGAACC 
CAACCTTATA AGGTGGCAGT TTATGTCCCA GGAAGTAAAG GTGCCCCCTC ATTTGTTCGA 
CTGTATCAGT ATCCCAACTT TGCCGGACCT CAGGCAGCAC TGGCCAATAA AAGCTTCTTT 
AAAGCTGATA AGGTTACAAT GCTGTGGAAT AAAAAAGCTA CTGCTGTGCT GGTAATAGCC 
AGTACAGAGG TTGACAAGAC AGGAGCCTCC TACTATGGAG AACAAACACT GCACTACATT 
GCAACAAATG GAGAAAGTGC TGTGGTACAA TTACCAAAAA ATGGCCCAAT TTATGATGTA 
GTTTGGAATT CCAGTTCTAC TGAGTTTTGC GCTGTTTATG GTTTTATGCC TGCGAAAGCA 
ACAGTTTTCA ACCTGAAGTG TGATCCTGTG TTTGACTTTG GGACTGGTCC TCGCAACGCA 
GCCTTCTATA GCCCTCATGG ACATATATTA GTGCTGGCTG GATTTGGAAA TCTTCGAGGA 
CAAATGGAAG TATGGGATGT TAAAAACTAC AAGCTTATTT CTAAGCCAGT GGCTTCTGAT 
TCTACATATT TTGCCTGGTG CCCAGATGGT GAGCATATCT TAACAGCCAC ATGTGCTCCT 
AGGTTACGTG TTAATAATGG GTATAAGATC TGGCATTATA CTGGCTCTCT TTTGCATAAG 
TATGATGTGC CATCAAATGG AGAATTATGG CAGGTTTCTT GGCAGCCATT TTTGGATGGA 
ATATTTCCAG CAAAAACGAT AAAGTACCAA GCAGTTCCAA GTGAAGTGCC TAGTGAGGAG 
CCTAAAGTTG CAACAGCTTA TAGACCTCCA GCCTTAAGAA ATAAGCCAGT CACCAATTCC 
AAACTGCATG AAGAGGAACC TCCCCAGAAT ATGAAGCCAC ATCCAGGAAG TGACAAGCCA 
TTATCAAAAA CAGCCCTTAA AAATCAAAGG AAGCATGAAG CTAAAAAAGC TGCAAAACAG 
GAAGCAAGAA GTGATGCGGC TCCTACTCCT GTCCCACAGA GTGCACCACG GAACACGGTC 
ACCCAGTCTG CTTCTGGTGA TCCTGAAGTA GACAAAAAAA TTAAGAATCT AAAGAAGAAA 
CTGAAAGCCA TAGAGCAGCT AAAAGAGCAG GCAGCCGCCG GGAAACAGCT AGAAAAAAAT 
CAGTTGGAGA AAATTCAGAA AGAGACAGCC CTTCTCCAAG AGCTTGAAGA TTTGGAATTG 
GGAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006501384.1
CDS287..1855
Translation

Target ORF information:

RefSeq Version XM_006501321.4
Organism Mus musculus(house mouse)
Definition Mus musculus eukaryotic translation initiation factor 2A (Eif2a), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006501321.4

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
ATGGACCACC ACACTTCACA GAAAGCACGG TGCTTCCCAG AGAATCTGGA AGAAATTGCA 
AAGTGTACAC TTTTAGTAAG GATGGGACAT TGTTTGCCTG GAGCAATGGA GAAAACTTCT 
AAAGATGGCA CTGCTGGGAC GCCTAACCTA CAACTTTATG ACATGAAAAC TGGAGCATGT 
TTGAAATCGT TCATCCAGAA AAAAATGCAA AATTGGTGTC CTTCCTGGTC AGATGATGAA 
ATTATTTGTG CCCGGAATGT TAACAACGAA GTTCACTTCT TTGAGAACAA CAATTTTAAC 
ACAATTGCAA ATAAATTACA TTTGCAAAAA GTTAATGACT TTAATTTATC ACCTGGAACC 
CAACCTTATA AGGTGGCAGT TTATGTCCCA GGAAGTAAAG GTGCCCCCTC ATTTGTTCGA 
CTGTATCAGT ATCCCAACTT TGCCGGACCT CAGGCAGCAC TGGCCAATAA AAGCTTCTTT 
AAAGCTGATA AGGTTACAAT GCTGTGGAAT AAAAAAGCTA CTGCTGTGCT GGTAATAGCC 
AGTACAGAGG TTGACAAGAC AGGAGCCTCC TACTATGGAG AACAAACACT GCACTACATT 
GCAACAAATG GAGAAAGTGC TGTGGTACAA TTACCAAAAA ATGGCCCAAT TTATGATGTA 
GTTTGGAATT CCAGTTCTAC TGAGTTTTGC GCTGTTTATG GTTTTATGCC TGCGAAAGCA 
ACAGTTTTCA ACCTGAAGTG TGATCCTGTG TTTGACTTTG GGACTGGTCC TCGCAACGCA 
GCCTTCTATA GCCCTCATGG ACATATATTA GTGCTGGCTG GATTTGGAAA TCTTCGAGGA 
CAAATGGAAG TATGGGATGT TAAAAACTAC AAGCTTATTT CTAAGCCAGT GGCTTCTGAT 
TCTACATATT TTGCCTGGTG CCCAGATGGT GAGCATATCT TAACAGCCAC ATGTGCTCCT 
AGGTTACGTG TTAATAATGG GTATAAGATC TGGCATTATA CTGGCTCTCT TTTGCATAAG 
TATGATGTGC CATCAAATGG AGAATTATGG CAGGTTTCTT GGCAGCCATT TTTGGATGGA 
ATATTTCCAG CAAAAACGAT AAAGTACCAA GCAGTTCCAA GTGAAGTGCC TAGTGAGGAG 
CCTAAAGTTG CAACAGCTTA TAGACCTCCA GCCTTAAGAA ATAAGCCAGT CACCAATTCC 
AAACTGCATG AAGAGGAACC TCCCCAGAAT ATGAAGCCAC ATCCAGGAAG TGACAAGCCA 
TTATCAAAAA CAGCCCTTAA AAATCAAAGG AAGCATGAAG CTAAAAAAGC TGCAAAACAG 
GAAGCAAGAA GTGATGCGGC TCCTACTCCT GTCCCACAGA GTGCACCACG GAACACGGTC 
ACCCAGTCTG CTTCTGGTGA TCCTGAAGTA GACAAAAAAA TTAAGAATCT AAAGAAGAAA 
CTGAAAGCCA TAGAGCAGCT AAAAGAGCAG GCAGCCGCCG GGAAACAGCT AGAAAAAAAT 
CAGTTGGAGA AAATTCAGAA AGAGACAGCC CTTCTCCAAG AGCTTGAAGA TTTGGAATTG 
GGAGTTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

HRD1 prevents apoptosis in renal tubular epithelial cells by mediating eIF2? ubiquitylation and degradation.
Cell death & disease8(12)3202(2017 Dec)
Huang Y,Sun Y,Cao Y,Sun H,Li M,You H,Su D,Li Y,Liang X


book

Neuroprotection by eIF2?-CHOP inhibition and XBP-1 activation in EAE/optic neuritiss.
Cell death & disease8(7)e2936(2017 Jul)
Huang H,Miao L,Liang F,Liu X,Xu L,Teng X,Wang Q,Ridder WH 3rd,Shindler KS,Sun Y,Hu Y