Pnmt cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pnmt
Entrez Gene ID	24661
Full Name	phenylethanolamine-N-methyltransferase
General protein information	Preferred Names phenylethanolamine-N-methyltransferase Names phenylethanolamine N-methyltransferase PNMTase noradrenaline N-methyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	catalyzes the conversion of norepinephrine to epinephrine in catecholamine biosynthesis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_031526.1	NP_113714.1	phenylethanolamine N-methyltransferase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PNMT	XP_511459.2
Canis lupus familiaris (dog)	PNMT	XP_548143.3
Xenopus tropicalis (tropical clawed frog)	pnmt	XP_002940421.1
Homo sapiens (human)	PNMT	NP_002677.1
	PNMT	XP_001089850.2
Mus musculus (house mouse)	Pnmt	NP_032916.1
Gallus gallus (chicken)	PNMT	XP_003642859.1
Rattus norvegicus (Norway rat)	Pnmt	NP_113714.1
Bos taurus (cattle)	PNMT	NP_803471.2
Danio rerio (zebrafish)	LOC100332609	XP_002666387.2

Gene Ontology
Bibliography

Related articles in PubMed

Prenatal glucocorticoid exposure programs adrenal PNMT expression and adult hypertension.
Nguyen P, Khurana S, Peltsch H, Grandbois J, Eibl J, Crispo J, Ansell D, Tai TC
The Journal of endocrinology227(2)117-27(2015 Nov)

Adolescent alcohol exposure alters the central brain circuits known to regulate the stress response.
Allen CD, Rivier CL, Lee SY
Neuroscience182162-8(2011 May)

Activation of different neuronal phenotypes in the rat brain induced by liver ischemia?reperfusion injury: dual Fos/neuropeptide immunohistochemistry.
Bundzikova J, Pirnik Z, Lackovicova L, Mravec B, Kiss A
Cellular and molecular neurobiology31(2)293-301(2011 Mar)

Regulation of catecholamine-synthesising enzymes and beta-adrenoceptors gene expression in ventricles of stressed rats.
Spasojevic N, Gavrilovic L, Dronjak S
Physiological research60 Suppl 1S171-6(2011)

Role of reactive oxygen species in the neural and hormonal regulation of the PNMT gene in PC12 cells.
Crispo JA, Ansell DR, Ubriaco G, Tai TC
Oxidative medicine and cellular longevity2011756938(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results suggest that increased PNMT gene expression via altered transcriptional activity is a possible mechanism by which prenatal exposure to elevated levels of glucocorticoids may program for hypertension later in life.
Title: Prenatal glucocorticoid exposure programs adrenal PNMT expression and adult hypertension.

Stress elevated PNMT levels in heart ventricles.
Title: Regulation of catecholamine-synthesising enzymes and beta-adrenoceptors gene expression in ventricles of stressed rats.

Findings demonstrate that CoCl(2)-induced ROS have synergistic effects on hormonal and neural activation of the PNMT promoter.
Title: Role of reactive oxygen species in the neural and hormonal regulation of the PNMT gene in PC12 cells.

Altered transcriptional regulation of PNMT is a contributing factor to altered adrenergic function in hypertension.
Title: Regulation of the phenylethanolamine N-methyltransferase gene in the adrenal gland of the spontaneous hypertensive rat.

Data show that voluntary wheel running failed to modify sensitivity to cardiovascular action of oxytocin but resulted in increased gene expression of PNMT in left, but not right, heart atrium in a running activity-dependent manner.
Title: Phenylethanolamine N-methyltransferase gene expression in the heart and blood pressure response to oxytocin treatment in rats exposed to voluntary wheel running.

The following Pnmt gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pnmt cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07986	NM_031526.1 Latest version!	Rattus norvegicus phenylethanolamine-N-methyltransferase (Pnmt), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pnmt ( NM_031526.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113714.1 Rattus norvegicus phenylethanolamine-N-methyltransferase (Pnmt), mRNA.

Price & Availability↑

CloneID	ORa07986
Clone ID Related Accession (Same CDS sequence)	NM_031526.1
Accession Version	NM_031526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	phenylethanolamine N-methyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X75333.1. On Oct 11, 2007 this sequence version replaced XM_001081395.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X14211.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACCGTG GCTCAGACCC GAAGCACACT GCAGGGATGG ACTCTGACTC CGACCCTGGC 
CAGGCAGAGG TCGCCTTGGC TTACCAGCGC TTTGAGCCCC GTGCCTACCT CCGCAACAAC 
TACGCGCCTC CTCGTGGGGA CCTGAGCAAC CCTGATGGTG TGGGGCCTTG GAAGCTTCGC 
TGCATGGCAC AAGTCTTTGC CACCGGTGAG GTGTCTGGAC AGGTCCTCAT TGACATCGGC 
TCAGGCCCCA CCATATACCA GCTGCTCAGC GCCTGTGCCC ACTTCGAGGA CATCACCATG 
ACAGACTTCT TGGAGGTCAA CCGGCAGGAG CTGGGACTCT GGCTGCGAGA GGAACCAGGA 
GCCTTCGACT GGAGTGTGTA TAGCCAGCAT GTCTGCCTCA TCGAGGACAA GGGAGAGTCC 
TGGCAGGAGA AAGAACGCCA GCTCCGAGCG AGGGTGAAGC GAGTCTTGCC CATTGATGTG 
CACAAGCCCC AGCCCCTGGG AGCTTCGGGC CTGGCACCCC TGCCTGCTGA CGCCTTGGTC 
TCTGCCTTCT GCCTGGAGGC TGTGAGCCCG GATCTCCCAA GCTTCCGGCA GGCTTTGTAT 
CATATCACGA CGCTGCTGAG GCCCGGGGGT AATCTCCTCT TCATCGGGGC CCTGGAGGAG 
TCGTGGTACC TTGCTGGGGA GGCCAGGCTA TCTGTGGTTC CAGTGTCAGA GGAGGAGGTG 
AGGGAGGCCC TGGTCTGTAG TGGTTATGAG GTCCGAGACC TTCGCACCTA CATCATGCCT 
GCCCACCTCC GCACGGGTGT GGATGACGTC AAGGGTATCT TCTTTGCCTG GGCCCAGAAG 
ATAGAGGTGC AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113714.1
CDS	21..878
Translation	MDRGSDPKHTAGMDSDSDPGQAEVALAYQRFEPRAYLRNNYAPPRGDLSNPDGVGPWKLRCMAQVFATGEVSGQVLIDIGSGPTIYQLLSACAHFEDITMTDFLEVNRQELGLWLREEPGAFDWSVYSQHVCLIEDKGESWQEKERQLRARVKRVLPIDVHKPQPLGASGLAPLPADALVSAFCLEAVSPDLPSFRQALYHITTLLRPGGNLLFIGALEESWYLAGEARLSVVPVSEEEVREALVCSGYEVRDLRTYIMPAHLRTGVDDVKGIFFAWAQKIEVQV

Target ORF information:

RefSeq Version	NM_031526.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phenylethanolamine-N-methyltransferase (Pnmt), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031526.1

ORF Insert Sequence:

ATGGACCGTG GCTCAGACCC GAAGCACACT GCAGGGATGG ACTCTGACTC CGACCCTGGC 
CAGGCAGAGG TCGCCTTGGC TTACCAGCGC TTTGAGCCCC GTGCCTACCT CCGCAACAAC 
TACGCGCCTC CTCGTGGGGA CCTGAGCAAC CCTGATGGTG TGGGGCCTTG GAAGCTTCGC 
TGCATGGCAC AAGTCTTTGC CACCGGTGAG GTGTCTGGAC AGGTCCTCAT TGACATCGGC 
TCAGGCCCCA CCATATACCA GCTGCTCAGC GCCTGTGCCC ACTTCGAGGA CATCACCATG 
ACAGACTTCT TGGAGGTCAA CCGGCAGGAG CTGGGACTCT GGCTGCGAGA GGAACCAGGA 
GCCTTCGACT GGAGTGTGTA TAGCCAGCAT GTCTGCCTCA TCGAGGACAA GGGAGAGTCC 
TGGCAGGAGA AAGAACGCCA GCTCCGAGCG AGGGTGAAGC GAGTCTTGCC CATTGATGTG 
CACAAGCCCC AGCCCCTGGG AGCTTCGGGC CTGGCACCCC TGCCTGCTGA CGCCTTGGTC 
TCTGCCTTCT GCCTGGAGGC TGTGAGCCCG GATCTCCCAA GCTTCCGGCA GGCTTTGTAT 
CATATCACGA CGCTGCTGAG GCCCGGGGGT AATCTCCTCT TCATCGGGGC CCTGGAGGAG 
TCGTGGTACC TTGCTGGGGA GGCCAGGCTA TCTGTGGTTC CAGTGTCAGA GGAGGAGGTG 
AGGGAGGCCC TGGTCTGTAG TGGTTATGAG GTCCGAGACC TTCGCACCTA CATCATGCCT 
GCCCACCTCC GCACGGGTGT GGATGACGTC AAGGGTATCT TCTTTGCCTG GGCCCAGAAG 
ATAGAGGTGC AGGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Prenatal glucocorticoid exposure programs adrenal PNMT expression and adult hypertension.
The Journal of endocrinology227(2)117-27(2015 Nov)
Nguyen P,Khurana S,Peltsch H,Grandbois J,Eibl J,Crispo J,Ansell D,Tai TC

Adolescent alcohol exposure alters the central brain circuits known to regulate the stress response.
Neuroscience182162-8(2011 May)
Allen CD,Rivier CL,Lee SY

Activation of different neuronal phenotypes in the rat brain induced by liver ischemia?reperfusion injury: dual Fos/neuropeptide immunohistochemistry.
Cellular and molecular neurobiology31(2)293-301(2011 Mar)
Bundzikova J,Pirnik Z,Lackovicova L,Mravec B,Kiss A

Regulation of catecholamine-synthesising enzymes and beta-adrenoceptors gene expression in ventricles of stressed rats.
Physiological research60 Suppl 1S171-6(2011)
Spasojevic N,Gavrilovic L,Dronjak S

Role of reactive oxygen species in the neural and hormonal regulation of the PNMT gene in PC12 cells.
Oxidative medicine and cellular longevity2011756938(2011)
Crispo JA,Ansell DR,Ubriaco G,Tai TC