Rims3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rims3
Entrez Gene ID	65025
Full Name	regulating synaptic membrane exocytosis 3
Synonyms	Nim2,Nim3
General protein information	Preferred Names regulating synaptic membrane exocytosis 3 Names regulating synaptic membrane exocytosis protein 3 RIM 3 RIM3 gamma rab-3-interacting molecule 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 5 Map Location: 5q36
Summary	mediates calcium-induced exocytosis [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022931.1	NP_075220.1	regulating synaptic membrane exocytosis protein 3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rims3	NP_891559.1
Canis lupus familiaris (dog)	RIMS3	XP_005628929.1
Danio rerio (zebrafish)	rims3	NP_001093479.1
Pan troglodytes (chimpanzee)	RIMS3	XP_003308066.1
Rattus norvegicus (Norway rat)	Rims3	NP_075220.1
Gallus gallus (chicken)	RIMS3	XP_004947844.1
Xenopus tropicalis (tropical clawed frog)	rims3	NP_001072491.1
Homo sapiens (human)	RIMS3	NP_055562.2
	RIMS3	XP_001084240.1
Bos taurus (cattle)	RIMS3	NP_001192796.1

Gene Ontology
Bibliography

Related articles in PubMed

RIM3? and RIM4? are key regulators of neuronal arborization.
Alvarez-Baron E, Michel K, Mittelstaedt T, Opitz T, Schmitz F, Beck H, Dietrich D, Becker AJ, Schoch S
The Journal of neuroscience : the official journal of the Society for Neuroscience33(2)824-39(2013 Jan)

Rab3-interacting molecule gamma isoforms lacking the Rab3-binding domain induce long lasting currents but block neurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca2+ channels.
Uriu Y, Kiyonaka S, Miki T, Yagi M, Akiyama S, Mori E, Nakao A, Beedle AM, Campbell KP, Wakamori M, Mori Y
The Journal of biological chemistry285(28)21750-67(2010 Jul)

RIM3gamma is a postsynaptic protein in the rat central nervous system.
Liang F, Zhang B, Tang J, Guo J, Li W, Ling EA, Chu H, Wu Y, Chan YG, Cao Q
The Journal of comparative neurology503(4)501-10(2007 Aug)

Genomic definition of RIM proteins: evolutionary amplification of a family of synaptic regulatory proteins.
Wang Y, S?dhof TC
Genomics81(2)126-37(2003 Feb)

The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins.
Wang Y, Sugita S, Sudhof TC
The Journal of biological chemistry275(26)20033-44(2000 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

gamma-RIMs are involved in cell biological functions distinct from the regulation of synaptic vesicle exocytosis and play a role in the molecular mechanisms controlling the establishment of dendritic complexity and axonal outgrowth
Title: RIM3? and RIM4? are key regulators of neuronal arborization.

The following Rims3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rims3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06445	NM_022931.1 Latest version!	Rattus norvegicus regulating synaptic membrane exocytosis 3 (Rims3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rims3 ( NM_022931.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_075220.1 Rattus norvegicus regulating synaptic membrane exocytosis 3 (Rims3), mRNA.

Price & Availability↑

CloneID	ORa06445
Clone ID Related Accession (Same CDS sequence)	NM_022931.1
Accession Version	NM_022931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 924bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Rattus norvegicus(Norway rat)
Product	regulating synaptic membrane exocytosis protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF199334.1. ##Evidence-Data-START## CDS exon combination :: AF199334.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN02642617, SAMN04563758 [ECO:0000350] ##Evidence-Data-END##

ATGTTTAACG GGGAGCCTGG TCCAGCCTCG GCTGGGGCCT CCAGGAATGT AGTGAGGAGC 
TCCAGCATCA GCGGTGAGAT CTGTGGATCC CAGCAAGCCG GAGGCGGGGC CGGGACCACC 
ACAGCCAAGA AACGGCGCAG CAGCCTGGGC GCCAAGATGG TGGCTATTGT CGGCTTGACC 
CAGTGGAGCA AAAGCACACT GCAGCTCCCC CAGCCTGAAG GGGCCACCAA GAAGCTGCGC 
AGCAACATTC GACGGAGCAC AGAGACGGGC ATTGCCGTGG AGATGCGGAG CCGTGTAACC 
CGCCAGGGCA GCCGGGAGTC TACCGATGGG AGCACCAACA GCAACAGCTC CGAGGGCACG 
TTTATTTTCC CCACACGGCT TGGAGCTGAA AGCCAGTTCA GTGACTTTCT GGATGGCCTG 
GGACCAGCCC AGATAGTGGG ACGACAGACA CTGGCCACAC CCCCCATGGG AGATGTGCAC 
ATTGCCATCA TGGACCGGAG TGGCCAGCTG GAGGTAGAAG TGATTGAAGC TCGGGGCCTG 
ACCCCCAAAC CAGGCTCCAA ATCCCTCCCA GCCACCTATA TCAAGGCTTA CCTGCTGGAG 
AACGGGGCAT GTGTGGCCAA GAAAAAGACA AAGGTGGCCA AGAAGACTTG TGATCCCTTG 
TACCAGCAAG CTTTGCTCTT TGATGAGGGG CCCCAAGGCA AGGTTCTACA GGTGATCGTC 
TGGGGAGACT ATGGCCGTAT GGACCACAAG TGCTTCATGG GTATGGCCCA GATCATGCTG 
GACGAGCTGG ACCTGAGTGC TGTGGTCACT GGCTGGTACA AATTCTTCCC CACATCCTCT 
GTGGCCGACT CCACACTTGG ATCCCTCACC AGGCGCCTGT CTCAGTCCTC CTTGGAGAGT 
GCCACCAGCC CCTCGTGCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_075220.1
CDS	444..1367
Translation	MFNGEPGPASAGASRNVVRSSSISGEICGSQQAGGGAGTTTAKKRRSSLGAKMVAIVGLTQWSKSTLQLPQPEGATKKLRSNIRRSTETGIAVEMRSRVTRQGSRESTDGSTNSNSSEGTFIFPTRLGAESQFSDFLDGLGPAQIVGRQTLATPPMGDVHIAIMDRSGQLEVEVIEARGLTPKPGSKSLPATYIKAYLLENGACVAKKKTKVAKKTCDPLYQQALLFDEGPQGKVLQVIVWGDYGRMDHKCFMGMAQIMLDELDLSAVVTGWYKFFPTSSVADSTLGSLTRRLSQSSLESATSPSCS

Target ORF information:

RefSeq Version	NM_022931.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus regulating synaptic membrane exocytosis 3 (Rims3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022931.1

ORF Insert Sequence:

ATGTTTAACG GGGAGCCTGG TCCAGCCTCG GCTGGGGCCT CCAGGAATGT AGTGAGGAGC 
TCCAGCATCA GCGGTGAGAT CTGTGGATCC CAGCAAGCCG GAGGCGGGGC CGGGACCACC 
ACAGCCAAGA AACGGCGCAG CAGCCTGGGC GCCAAGATGG TGGCTATTGT CGGCTTGACC 
CAGTGGAGCA AAAGCACACT GCAGCTCCCC CAGCCTGAAG GGGCCACCAA GAAGCTGCGC 
AGCAACATTC GACGGAGCAC AGAGACGGGC ATTGCCGTGG AGATGCGGAG CCGTGTAACC 
CGCCAGGGCA GCCGGGAGTC TACCGATGGG AGCACCAACA GCAACAGCTC CGAGGGCACG 
TTTATTTTCC CCACACGGCT TGGAGCTGAA AGCCAGTTCA GTGACTTTCT GGATGGCCTG 
GGACCAGCCC AGATAGTGGG ACGACAGACA CTGGCCACAC CCCCCATGGG AGATGTGCAC 
ATTGCCATCA TGGACCGGAG TGGCCAGCTG GAGGTAGAAG TGATTGAAGC TCGGGGCCTG 
ACCCCCAAAC CAGGCTCCAA ATCCCTCCCA GCCACCTATA TCAAGGCTTA CCTGCTGGAG 
AACGGGGCAT GTGTGGCCAA GAAAAAGACA AAGGTGGCCA AGAAGACTTG TGATCCCTTG 
TACCAGCAAG CTTTGCTCTT TGATGAGGGG CCCCAAGGCA AGGTTCTACA GGTGATCGTC 
TGGGGAGACT ATGGCCGTAT GGACCACAAG TGCTTCATGG GTATGGCCCA GATCATGCTG 
GACGAGCTGG ACCTGAGTGC TGTGGTCACT GGCTGGTACA AATTCTTCCC CACATCCTCT 
GTGGCCGACT CCACACTTGG ATCCCTCACC AGGCGCCTGT CTCAGTCCTC CTTGGAGAGT 
GCCACCAGCC CCTCGTGCTC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RIM3? and RIM4? are key regulators of neuronal arborization.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(2)824-39(2013 Jan)
Alvarez-Baron E,Michel K,Mittelstaedt T,Opitz T,Schmitz F,Beck H,Dietrich D,Becker AJ,Schoch S

Rab3-interacting molecule gamma isoforms lacking the Rab3-binding domain induce long lasting currents but block neurotransmitter vesicle anchoring in voltage-dependent P/Q-type Ca2+ channels.
The Journal of biological chemistry285(28)21750-67(2010 Jul)
Uriu Y,Kiyonaka S,Miki T,Yagi M,Akiyama S,Mori E,Nakao A,Beedle AM,Campbell KP,Wakamori M,Mori Y

RIM3gamma is a postsynaptic protein in the rat central nervous system.
The Journal of comparative neurology503(4)501-10(2007 Aug)
Liang F,Zhang B,Tang J,Guo J,Li W,Ling EA,Chu H,Wu Y,Chan YG,Cao Q

Genomic definition of RIM proteins: evolutionary amplification of a family of synaptic regulatory proteins.
Genomics81(2)126-37(2003 Feb)
Wang Y,S?dhof TC

The RIM/NIM family of neuronal C2 domain proteins. Interactions with Rab3 and a new class of Src homology 3 domain proteins.
The Journal of biological chemistry275(26)20033-44(2000 Jun)
Wang Y,Sugita S,Sudhof TC