Anti-idiotype Antibody Services

Guaranteed application in immunoassay development for PK and ADA analysis

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Overview

An anti-idiotype antibody (anti-ID Ab, see the schematic diagram) binds to the idiotype of another antibody, usually an antibody-drug. An idiotype can be defined as the specific combination of idiotopes present within an antibody complementary determining regions. Since anti-ID Abs are capable of binding to antibody-drugs within biological fluids, they are commonly used in the preclinical setting to analyze the pharmacokinetics (PK) and pharmacodynamics (PD) of antibody-drugs. Due to the similarity between anti-ID Abs and anti-drug antibodies, anti-ID Abs are also commonly used as a reference standard for antibody-drug immunogenicity, anti-drug antibody (ADA) studies, and neutralization antibody measurements.

Leveraging its extensive expertise in antibody development, GenScript partners with investigators just like you in developing highly sensitive and specific polyclonal and monoclonal anti-ID antibodies to best suit your particular downstream applications.

Antibody Services

Type	1. Antigen-blocking	2. Non-blocking	3. Complex-specific
Schematic diagram
Feature	Paratope-specific	Not Paratope-specific	Partially paratope-specific
Antigen binding	Inhibitory	Not inhibitory	Not inhibitory
Application	Detect free drug only	Detect total drug (free and bound)	Detect bound drug typically
Selection Guideline	Type 1 or type 2 could be applied to detect antibody drug when having a cell-bound drug target e.g. PD1/PDL1 Type 1 & type 2 may both be needed when having a secret drug target e.g. TNF

Highlights

High-quality Delivery

Antibody purity > 90%
ELISA > 729K
Cross-reaction < 2%

Fast Turnaround Time

8 weeks to deliver purified Anti-ID pAb
Patented express immunization technology

Extensive Expertise

> 18 years of global service experience
> 99.5% success rate of anti-ID projects

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Services Details

Anti-ID Polyclonal Antibody Package

Guaranteed Anti-ID Rabbit Polyclonal Antibody Package
Guaranteed Amount	5/10/15/20 mg >20 mg, please request a quote
Target type	Full-length IgG/ADC Bi-specific Ab	scFv/VHH	RNA/VLP (Neo)peptide/recombinant protein/small molecule/Cell etc
Phase I Immunogen preparation	F(ab’) 2 preparation or whole antibody/ADC	scFv/VHH	KLH/BSA conjugation or whole protein/cell
Phase II Animal immunization	Express immunization protocol (Phoenix) with rabbits [OLAW&AAALAC-accredited facility]	Express immunization protocol (Phoenix) with rabbits [OLAW&AAALAC-accredited facility]	Conventional immunization protocol with rabbits [OLAW&AAALAC-accredited facility]
Phase III Purification & QC	Two-step/three-step purification	Two-step purification	One-step purification
TAT	8-11 wks	8-11 wks	10-13 wks

Anti-ID Monoclonal Antibody Package

Anti-ID Mouse Monoclonal Antibody Package

Customized Anti-ID Mouse Monoclonal Antibody Package
Target type		Full length IgG	scFv/VHH/RNA/VLP/(Neo)peptide/recombinant protein/small molecule/Cell etc.	ADC/Bi-specific Ab
Phase I Immunogen preparation		F(ab’) 2 preparation	KLH conjugation	Fab preparation/KLH conjugation
Phase II Animal immunization		Conventional immunization protocol Express immunization protocol (MonoBoost) ^Preferred
Phase III Cell fusion and screening		Cell fusion & one round of subcloning Deliver supernatants of 20 clones, 2ml/clone Confirm the type of anti-ID mAb
Phase IV Antibody production	Option 1 Sequencing & rAb production ^Preferred	5 clones sequencing by NGS Guaranteed 0.1mg recombinant production and ELISA validation
Phase IV Antibody production	Option 2 Clone delivery & Ab hybridoma production	Subcloning for up to 5 clones Clone storage (Optional) Ab hybridoma production and purification
TAT		13-17 wks*	13-16 wks*	13-17 wks*

*: TAT is calculated based on preferred process.

Anti-ID Rabbit Monoclonal Antibody Package

Customized Anti-ID Rabbit Monoclonal Antibody Package
Target type		Full length IgG/ADC/Bi-specific Ab	scFv/VHH	RNA/VLP/(Neo)peptide /recombinant protein/small molecule/Cell etc.
Phase I Immunogen preparation		F(ab’) 2 preparation or whole antibody/ADC	Whole scFv/VHH	KLH/BSA conjugation or whole protein/cell
Phase II Animal immunization		Conventional immunization protocol Express immunization protocol (Phoenix) ^Preferred
Phase III B cell cloning and screening	Blocking format	Blocking ELISA for Indirect ELISA-positive clones Deliver 1 ml supernatants/clone for up to 20 ELISA-positive clones 1-10 blockers & blocking ELISA report.
Phase III B cell cloning and screening	Blocking and non-blocking formats	Blocking ELISA for Indirect ELISA-positive clones Deliver 1 ml supernatants/clone for up to 50 ELISA-positive clones 1-10 blockers & non-blockers or only non-blockers & blocking ELISA report
Phase IV Sequencing & rAb production		5 clones sequencing by Sanger Guaranteed 0.1mg recombinant production and ELISA positive validation
TAT		13-15 wks	13-14 wks	13-15 wks

Case Studies

Anti-ID Polyclonal Antibody

Case 1: Against peptide-drug, 5 rabbits, 16.42 mg purified pAb

Immunogen: Peptide conjugated with KLH

Immunization Protocol: Rabbit express immunization protocol (Phoenix)

After immunization, the serum titer > 512K, the serum inhibition efficiency＞15%

The results show that both the serum and purified pAb have good binding and inhibition abilities to the peptide-drug.

Anti-ID Mouse Monoclonal Antibody

Case 1: Against antibody-drug, 20 clones were selected for blocking application

Project Target: Screening anti-ID mouse antibody for blocking and non-blocking applications

Immunogen: F(ab’) 2 digested by Pepsin

Immunization Protocol: Mouse express immunization protocol (Monoboost)

Clone	C	1	2	3	4	5	6	7	8	9	10
Average OD	1.512	0.213	0.179	0.239	0.281	0.171	0.205	0.226	0.239	0.158	0.127
Inhibition Efficiency（%）	N/A	85.9	88.2	84.2	81.4	88.7	86.4	85.1	84.2	89.6	91.6

Clone	C	11	12	13	14	15	16	17	18	19	20
Average OD	1.512	0.175	0.196	0.261	0.171	0.228	1.631	1.606	1.699	1.115	1.244
Inhibition Efficiency（%）	N/A	88.4	87.0	82.7	88.7	18.8	-7.9	-6.2	-12.4	26.3	17.7

Anti-ID Rabbit Monoclonal Antibody

Case 1: Against peptide-drug, 9 clones were selected for blocking application

Project Target: Screeninganti-ID rabbit antibody for block application

Immunogen: Peptide conjugated with KLH

Immunization Protocol: Rabbit Express immunization protocol (Phoenix)

Clone C 1 2 3 4 5

Average OD 1.527 1.184 1.305 1.345 1.348 1.363

Inhibition Efficiency（%） N/A 22.5% 14.5% 11.9% 11.7% 10.7%

Clone 6 7 8 9 10 11

Average OD 1.381 1.383 1.385 1.39 1.655 1.663

Inhibition Efficiency（%） 9.6% 9.4% 9.3% 9.0% -8.4% -8.9%

Clone 12 13 14 15 16 17

Average OD 1.671 1.701 1.731 1.727 1.752 1.82

Inhibition Efficiency（%） -9.4% -11.4% -12.2% -13.1% -14.7% -19.2%

*: The IC50 was not calculated because the clone was not sufficiently diluted.

Clone	C	1	2	3	4	5
Average OD	1.527	1.184	1.305	1.345	1.348	1.363
Inhibition Efficiency（%）	N/A	22.5%	14.5%	11.9%	11.7%	10.7%

Clone	6	7	8	9	10	11
Average OD	1.381	1.383	1.385	1.39	1.655	1.663
Inhibition Efficiency（%）	9.6%	9.4%	9.3%	9.0%	-8.4%	-8.9%

Clone	12	13	14	15	16	17
Average OD	1.671	1.701	1.731	1.727	1.752	1.82
Inhibition Efficiency（%）	-9.4%	-11.4%	-12.2%	-13.1%	-14.7%	-19.2%

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Anti-idiotype Antibody Services

Overview