GenScript's standard polyclonal antibody services packages use traditional immunization protocols and allow custom polyclonal antibody and antiserum production in rabbits, mice, and rats. Multiple antibody purification options are available and include: affinity purification, protein A/G and IgY purification or just antiserum. The availability of large animal species allows polyclonal antibody and antiserum production in large quantities and choice of animal host grants the use of unrelated animal species to ensure the specificity of your polyclonal antibody.
Record Fast
Turn-Around-Times
Higher Purified
pAb Yields!
Market High ELISA
Titers of 1:256,000
Advanced Antigen
Design Tool
Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.
These services generate custom rabbit polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool.
Standard Protocol (Two animals)
| Steps | Time from Project Initiation | Time from Immunization | Activity Description |
|---|---|---|---|
| Peptide synthesis | ≈2 weeks | Day 21 | - |
| Peptide conjugation to carrier | ≈3 weeks | Day 7 | - |
| Primary immunization | ≈4 weeks | Day 0 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Complete Adjuvant, Subcutaneous administration |
| 1st boost | ≈6 weeks | Day 14 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration |
| 2nd boost | ≈8 weeks | Day 28 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration |
| Production bleed | ≈9 weeks | Day 35 | 10-20 ml serum per rabbit |
| 3rd boost | ≈10 weeks | Day 42 | 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration |
| Production bleed | ≈11 weeks | Day 49 | 40 ml serum per rabbit |
| ELISA, purification, and shipping | ≈13 weeks | Day 63 | Extend or terminate |
These services generate custom mouse polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool.
Standard Protocol (Five animals)
| Steps | Time from Project Initiation | Time from Immunization | Activity Description |
|---|---|---|---|
| Peptide synthesis | ≈2 weeks | Day 21 | - |
| Peptide conjugation to carrier | ≈3 weeks | Day 7 | - |
| Primary immunization | ≈4 weeks | Day 0 | 50 μg per mouse peptide-KLH conjugate, Freud's Complete Adjuvant, Intrapreitoneal injection. |
| 1st boost | ≈6 weeks | Day 14 | 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection. |
| 2nd boost | ≈8 weeks | Day 28 | 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection. |
| Test bleed and ELISA | ≈9 weeks | Day 35 | 0.01-0.02 ml serum per mouse. ELISA and response evaluation. |
| 3rd boost | ≈10 weeks | Day 42 | 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection. |
| Production bleed | ≈11 weeks | Day 49 | 0.5 ml serum per mouse. |
| ELISA, purification, and shipping | ≈13 weeks | Day 63 | Extend or terminate |
Note: If no specific request, 0.02% w/v sodium azide
will be added in the antisera and antibody products to prevent bacteria
growth.
*During the development of the antibody, if there is the death of the experimental
animal, we will choose the adjusted immunization process for re-immunization to ensure the
high quality and efficiency of the order.
These services generate custom rat polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool. Both rat antibody packages come with a guaranteed ELISA titer of ≥1:256,000.
Standard Protocol (Three animals)
| Steps | Time from Project Initiation | Time from Immunization | Activity Description |
|---|---|---|---|
| Peptide synthesis | ≈2 weeks | Day 21 | - |
| Peptide conjugation to carrier | ≈3 weeks | Day 7 | - |
| Primary immunization | ≈4 weeks | Day 0 | 50 µg per rat peptide-KLH conjugate, -Freunds's Complete Adjuvant, Intraperitoneal injection |
| 1st boost | ≈6 weeks | Day 14 | 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection |
| 2nd boost | ≈8 weeks | Day 28 | 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection |
| Test bleed and ELISA | ≈9 weeks | Day 35 | 0.01-0.02 ml serum per rat. ELISA and initial response evaluation. |
| 3rd boost | ≈10 weeks | Day 42 | 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection |
| Production bleed | ≈11 weeks | Day 49 | 1.5 – 2 ml serum per rat |
| ELISA, purification, and shipping | ≈13 weeks | Day 63 | Extend or terminate |
Note: If no specific request, 0.02% w/v sodium azide
will be added in the antisera and antibody products to prevent bacteria
growth.
During the development of the antibody, if there is the death of the experimental
animal, we will choose the adjusted immunization process for re-immunization to ensure the
high quality and efficiency of the order.
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.
