Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.
The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)
Cost effective and comprehensive
pricing starts from $899 and includes antigen synthesis and purification!
Fast turnaround time
delivery of purified custom polyclonal antibodies in as little as 45 days.
Optimal immunization
Our OptimumAntigen design tool and intelligent Antigen Strategy increase specificity and affinity of antibodies.
Guaranteed results
affinity purified custom polyclonal antibody quantities and ELISA titers of 1:256,000.
Certified facility
AAALAC International accreditation and OLAW certification, demonstrating our commitment to responsible animal care and use.
Details of PolyExpress™ Custom Polyclonal Antibody Production Services
| Feature | Details | SC1180 | SC1676 |
|---|---|---|---|
| Immunization | Antigen Synthesized | 1 peptide | 1 protein |
| No. of Rabbits Immunized | 2 | ||
| Deliverables | Negative Control | Total IgG (optional: pre-immune serum or total serum protein delivery) | |
| Antigen Sample | 2 mg peptide antigen | 200 μg protein | |
| Antigen Affinity Purified pAb(s) | 1 (2-6 mg total) | 1 (2-10 mg antigen affinity purified total antibody) | |
| Guaranteed Items | Application | ELISA titer 1:256,000 | Dot Blot/WB positive for target antigen binding; ELISA titer 1:256,000 |
| Antibody Yield | 1 purified pAb ≥ 2 mg | 2-10 mg antigen affinity purified total antibody | |
| Timeline | 45 days | 10-11 weeks | |
*GenScript provides peptide synthesis, peptide design, carrier conjugation and antibody purification for
free.
**During the development of the antibody, if there is the death of the experimental animal, we will choose
the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the
order.
Western blot analyses were performed to measure the effect of the Osi21 functional loss on the degradation of endocytosed rhodopsin.
The polyclonal rabbit antiarrestin2 antibody is diluted 1:30,000. (GenScript, NY)
Antibodies produced using PolyExpress™ have ELISA titers as high as polyclonal antibodies produced using a traditional protocol.
ELISA analysis of purified antibody generated by either PolyExpress™ protocol or traditional protocol.
ELISA analysis of purified antibody generated by PolyExpress™ Antibody
Service.
PolyExpress™ generated an antibody with ELISA titer> 1: 256,000 which is higher than guaranteed.
| Assays | Western Blot | Sandwich ELISA | IF, IHC, Flow Cytometry |
IP | ChIP |
|---|---|---|---|---|---|
| Requirements&Rating Rationale |
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| SC1676 | 
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| SC1180 | 
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For polyclonal antibodies targeting poorly defined epitopes we recommend protein antigens. Protein antigens provide the immune system the complete profile of exposed epitopes in the same conformational context the antibody will ultimately recognize the antigen such as FACS, sandwich ELISA, or receptor blocking for example.
High quality, purified monoclonal antibodies guaranteed to work in your application.
A novel rabbit mAb discovery platform that delivers antibodies of high target specificity & affinity for your research application.
Generating the Yield You Need With The Speed You Want!
Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.
