GenCRISPR SpCas9 demonstrated high gene editing efficiency in both in vitro cleavage tests and HEK293T cells.
In vitro cleavage test

Gene knockout efficiency_HEK293 T cell

Wild-type Cas9 protein engineered for optimal CRISPR editing performance
Applications: Suitable for a wide range of CRISPR gene editing experiments.
High-fidelity Cas9 variant engineered for enhanced specificity without compromising efficiency
Applications: Ideal for CRISPR experiments that need precise editing or low off-target effects.
GMP-grade GenCRISPR™ SpCas9 and Ultra eSpCas9
Wild-type and high-fidelity Cas9 produced to meet the requirements for clinical development
Applications: Ideal for CRISPR projects that aim for therapeutic applications.
GenCRISPR SpCas9 demonstrated high gene editing efficiency in both in vitro cleavage tests and HEK293T cells.
In vitro cleavage test
Gene knockout efficiency_HEK293 T cell
Gene knockout efficiency_293 T cells & Jurkat cells
GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency of three batches shows no significant difference.
GenCRISPR SpCas9 is used for gene knockout in 293T cells and Jurkat cells. As shown in the figure, the gene knockout efficiency is equivalent to that of the leading supplier I.
The fidelity and editing efficiency of GenCRISPR eSpCas9 Protein were evaluated against wildtype Cas9 on Jurkat cells and primary T cells. GenCRISPR eSpCas9 exhibited a superior ability to reduce off-target edits.
Low off-target effects_Jurkat cells
Low off-target effects_ Primary T cells
GenCRISPR eSpCas9 exhibits comparable knockout and knock-in efficiency to the wildtype SpCas9 in both Jurkat cells and primary T cells.
High Knockout_ Jurkat cells and Primary T cell
High Knock-in_ Primary T cell
The GenCRIPSR™ Ultra eSpCas9 (mt) shows high batch-to-batch consistency, with no significant differences in cell viability and consistent knockout/knock-in efficiency across the three batches at any concentration.
KO at TRAC sites in HEK293T cells
GFP KI at RAB11A in Jurkat cells
GenCRISPR eSpCas9 exhibits lower off-target effects compared to HiFi Cas9 products from leading suppliers, while its knockout and knock-in efficiencies are equivalent to or exceed those of the leading suppliers.
Low off-target effects_HEK293 T cell
High Knockout efficiency_HEK293 T cell
High Knock-in efficiency_Primary T cell
For the seamless transition from research to clinical practice, high-quality materials and thorough documentation are essential. GenCRISPR GMP-grade Cas9 proteins are crafted to strict standards to aid in your cell therapy development projects.
In addition to our catalog of Cas9 products, GenScript provides customized CRISPR Cas Nuclease services. We can produce nucleases for a variety of CRISPR systems, including MAD7, Cas12a, Cas13, and Cas-mini to base editor/prime editor configurations. For more details, visit our custom CRISPR Cas Nuclease page or fill out the contact form.