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DHCR7 cDNA ORF clone, Homo sapiens (human)

Gene Symbol DHCR7
Entrez Gene ID 1717
Full Name 7-dehydrocholesterol reductase
Synonyms SLOS
General protein information
Preferred Names
7-dehydrocholesterol reductase
Names
7-dehydrocholesterol reductase
7-DHC reductase
sterol delta-7-reductase
putative sterol reductase SR-2
delta-7-dehydrocholesterol reductase
NP_001157289.1
NP_001351.2
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

11

11q13.4

Summary This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by mental retardation, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]. lac of sum
Disorder MIM:

602858

Disorder Html: Smith-Lemli-Opitz syndrome, 270400 (3)

mRNA and Protein(s)

mRNA Protein Name
XM_011544777 XP_011543079 7-dehydrocholesterol reductase isoform X1
NM_001360 NP_001351 7-dehydrocholesterol reductase
NM_001163817 NP_001157289 7-dehydrocholesterol reductase



Homo sapiens (human) DHCR7 NP_001157289.1
Pan troglodytes (chimpanzee) DHCR7 XP_001174206.1
Canis lupus familiaris (dog) DHCR7 XP_005631421.1
Bos taurus (cattle) DHCR7 NP_001014927.1
Mus musculus (house mouse) Dhcr7 NP_031882.1
Rattus norvegicus (Norway rat) Dhcr7 NP_071784.1
Gallus gallus (chicken) DHCR7 NP_001186419.1
Danio rerio (zebrafish) dhcr7 NP_958487.1
Arabidopsis thaliana (thale cress) DWF5 NP_175460.1
Xenopus (Silurana) tropicalis (western clawed frog) dhcr7 NP_989235.1


GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following DHCR7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DHCR7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu57236 XM_011544777 PREDICTED: Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu21071
NM_001360 Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00
OHu21071
NM_001163817 Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector
in pcDNA3.1+/C-(K)DYK
$49.50-$69.30
$99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu57236
Clone ID Related Accession (Same CDS sequence) XM_011544777
Accession Version XM_011544777.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product 7-dehydrocholesterol reductase isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_167190.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGAAGAGAA CACGGAGGCA AGGCGTGTGT CAGAGGCAGA GCTGGGGTTT GACCCCAGGC
CGCTGGGCCC CCGAGCCCAC ACTCCTGTCC TCTCCCTGGG CAAAGCACCG CTTGACCCCT
TCCCCCTCGC CCCCCAGGGT CTGTACTTGG TGTACCACCC CGTGCAGCTG TCCACCCCGC
ACGCCGTGGG CGTCCTGCTG CTGGGCCTGG TGGGCTACTA CATCTTCCGG GTGGCCAACC
ACCAGAAGGA CCTGTTCCGC CGCACGGATG GGCGCTGCCT CATCTGGGGC AGGAAGCCCA
AGGTCATCGA GTGCTCCTAC ACATCCGCCG ATGGGCAGAG GCACCACAGC AAGCTGCTGG
TGTCGGGCTT CTGGGGCGTG GCCCGCCACT TCAACTACGT CGGCGACCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011543079.1
CDS224..1594
Misc Feature(1)500..>1192(+)
Translation

Target ORF information:

RefSeq Version XM_011544777
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011544777

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGAAGAGAA CACGGAGGCA AGGCGTGTGT CAGAGGCAGA GCTGGGGTTT GACCCCAGGC
CGCTGGGCCC CCGAGCCCAC ACTCCTGTCC TCTCCCTGGG CAAAGCACCG CTTGACCCCT
TCCCCCTCGC CCCCCAGGGT CTGTACTTGG TGTACCACCC CGTGCAGCTG TCCACCCCGC
ACGCCGTGGG CGTCCTGCTG CTGGGCCTGG TGGGCTACTA CATCTTCCGG GTGGCCAACC
ACCAGAAGGA CCTGTTCCGC CGCACGGATG GGCGCTGCCT CATCTGGGGC AGGAAGCCCA
AGGTCATCGA GTGCTCCTAC ACATCCGCCG ATGGGCAGAG GCACCACAGC AAGCTGCTGG
TGTCGGGCTT CTGGGGCGTG GCCCGCCACT TCAACTACGT CGGCGACCTG A

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21071
Clone ID Related Accession (Same CDS sequence) NM_001163817 , NM_001360
Accession Version NM_001360.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 7-dehydrocholesterol reductase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA502590.1, BU848891.1, AF062481.1 and AP002387.4. This sequence is a reference standard in the RefSeqGene project. On Dec 25, 2006 this sequence version replaced gi:4503320. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF034544.1, AK289497.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001351.2
CDS274..1701
Misc Feature(1)193..195(+)
Misc Feature(2)313..315(+)
Misc Feature(3)391..453(+)
Misc Feature(4)550..1698(+)
Misc Feature(5)733..795(+)
Misc Feature(6)802..864(+)
Misc Feature(7)1069..1131(+)
Misc Feature(8)1189..1251(+)
Misc Feature(9)1264..1326(+)
Misc Feature(10)1531..1593(+)
Exon (1)1..142
Gene:DHCR7
Gene Synonym:
Exon (2)143..267
Gene:DHCR7
Gene Synonym:
Exon (3)268..371
Gene:DHCR7
Gene Synonym:
Exon (4)372..594
Gene:DHCR7
Gene Synonym:
Exon (5)595..685
Gene:DHCR7
Gene Synonym:
Exon (6)686..899
Gene:DHCR7
Gene Synonym:
Exon (7)900..1104
Gene:DHCR7
Gene Synonym:
Exon (8)1105..1236
Gene:DHCR7
Gene Synonym:
Exon (9)1237..2665
Gene:DHCR7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001360
Organism Homo sapiens (human)
Definition Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001360

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu21071
Clone ID Related Accession (Same CDS sequence) NM_001163817 , NM_001360
Accession Version NM_001163817.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1428bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags COA
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product 7-dehydrocholesterol reductase
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA502590.1, AP002387.4 and AI888720.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC000054.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001157289.1
CDS251..1678
Misc Feature(1)170..172(+)
Misc Feature(2)290..292(+)
Misc Feature(3)368..430(+)
Misc Feature(4)527..1675(+)
Misc Feature(5)710..772(+)
Misc Feature(6)779..841(+)
Misc Feature(7)1046..1108(+)
Misc Feature(8)1166..1228(+)
Misc Feature(9)1241..1303(+)
Misc Feature(10)1508..1570(+)
Exon (1)1..119
Gene:DHCR7
Gene Synonym:
Exon (2)120..244
Gene:DHCR7
Gene Synonym:
Exon (3)245..348
Gene:DHCR7
Gene Synonym:
Exon (4)349..571
Gene:DHCR7
Gene Synonym:
Exon (5)572..662
Gene:DHCR7
Gene Synonym:
Exon (6)663..876
Gene:DHCR7
Gene Synonym:
Exon (7)877..1081
Gene:DHCR7
Gene Synonym:
Exon (8)1082..1213
Gene:DHCR7
Gene Synonym:
Exon (9)1214..2642
Gene:DHCR7
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001163817
Organism Homo sapiens (human)
Definition Homo sapiens 7-dehydrocholesterol reductase (DHCR7), transcript variant 2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001163817

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
ATGGCTGCAA AATCGCAACC CAACATTCCC AAAGCCAAGA GTCTAGATGG CGTCACCAAT 
GACAGAACCG CATCTCAAGG GCAGTGGGGC CGTGCCTGGG AGGTGGACTG GTTTTCACTG
GCGAGCGTCA TCTTCCTACT GCTGTTCGCC CCCTTCATCG TCTACTACTT CATCATGGCT
TGTGACCAGT ACAGCTGCGC CCTGACTGGC CCTGTGGTGG ACATCGTCAC CGGACATGCT
CGGCTCTCGG ACATCTGGGC CAAGACTCCA CCTATAACGA GGAAAGCCGC CCAGCTCTAT
ACCTTGTGGG TCACCTTCCA GGTGCTTCTG TACACGTCTC TCCCTGACTT CTGCCATAAG
TTTCTACCCG GCTACGTAGG AGGCATCCAG GAGGGGGCCG TGACTCCTGC AGGGGTTGTG
AACAAGTATC AGATCAATGG CCTGCAAGCC TGGCTCCTCA CGCACCTGCT CTGGTTTGCA
AACGCTCATC TCCTGTCCTG GTTCTCGCCC ACCATCATCT TCGACAACTG GATCCCACTG
CTGTGGTGCG CCAACATCCT TGGCTATGCC GTCTCCACCT TCGCCATGGT CAAGGGCTAC
TTCTTCCCCA CCAGCGCCAG AGACTGCAAA TTCACAGGCA ATTTCTTTTA CAACTACATG
ATGGGCATCG AGTTTAACCC TCGGATCGGG AAGTGGTTTG ACTTCAAGCT GTTCTTCAAT
GGGCGCCCCG GGATCGTCGC CTGGACCCTC ATCAACCTGT CCTTCGCAGC GAAGCAGCGG
GAGCTCCACA GCCATGTGAC CAATGCCATG GTCCTGGTCA ACGTCCTGCA GGCCATCTAC
GTGATTGACT TCTTCTGGAA CGAAACCTGG TACCTGAAGA CCATTGACAT CTGCCATGAC
CACTTCGGGT GGTACCTGGG CTGGGGCGAC TGTGTCTGGC TGCCTTATCT TTACACGCTG
CAGGGTCTGT ACTTGGTGTA CCACCCCGTG CAGCTGTCCA CCCCGCACGC CGTGGGCGTC
CTGCTGCTGG GCCTGGTGGG CTACTACATC TTCCGGGTGG CCAACCACCA GAAGGACCTG
TTCCGCCGCA CGGATGGGCG CTGCCTCATC TGGGGCAGGA AGCCCAAGGT CATCGAGTGC
TCCTACACAT CCGCCGATGG GCAGAGGCAC CACAGCAAGC TGCTGGTGTC GGGCTTCTGG
GGCGTGGCCC GCCACTTCAA CTACGTCGGC GACCTGATGG GCAGCCTGGC CTACTGCCTG
GCCTGTGGCG GCGGCCACCT GCTGCCCTAC TTCTACATCA TCTACATGGC CATCCTGCTG
ACCCACCGCT GCCTCCGGGA CGAGCACCGC TGCGCCAGCA AGTACGGCCG GGACTGGGAG
CGCTACACCG CCGCAGTGCC TTACCGCCTG CTGCCTGGAA TCTTCTAA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

 
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