Hace1 cDNA ORF clone, Mus musculus(house mouse)

  • Gene

  • Clones

  • gRNAs

The following Hace1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hace1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OMu21882 NM_172473.3
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $475.30
$679.00
OMu43797 XM_006512634.4
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu43798 XM_006512635.4
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu43799 XM_006512636.4
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu43800 XM_006512637.4
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OMu43801 XM_006512638.4
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu72690 XM_030244983.1
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OMu72691 XM_017313865.2
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OMu85395 XM_030244982.1
Latest version!
Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OMu72691 XM_017313865.1 Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $307.30
$439.00
OMu43799 XM_006512636.3 Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu43797 XM_006512634.3 Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu43798 XM_006512635.3 Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu43800 XM_006512637.3 Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OMu43801 XM_006512638.3 Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $391.30
$559.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OMu72691
    Clone ID Related Accession (Same CDS sequence) XM_017313865.1 , XM_017313865.2
    Accession Version XM_017313865.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT TCGGGAAAAC ATGTTACGGC AAGTTCTGCA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATC CTGACAGGCC TTGCTGAAGT TGCTACGACA
    AATGGCCATA AGCTGCTTAG TTTATCTAGC AATTATGATG CGCAGATGAA GAGCCTTTTG
    AGGATTGTGA GGATATTTTG TCATGTCTTT CGAATTGGTC CCTCATCTCC CAGTAATGGA
    ATCGATATGG GCTACAATGG AAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTTTGGAA
    TTGCTTTGGC ACTCATTGGA TGAATGGTTA GTTTTAATAG CCACAGAATT AATGAAAAAC
    AAAGAGGATT CAACAGATAT TACTTCTATC TTGCTGAAAC AAAAAGGCCA GGATCAAGAG
    GCCCCTTCTC TTTCTGCATT TGAGCCACCA GGCCCTGGGA GCTATGAAAG CCTGCCCCCT
    GGTCCAGGGG ACTCCAAGCC AGAGGTGCTT GCAGGGGAGC AGGAAGCCAG TGCCGACTGT
    CAAGACGTCA TCTCTGTGAC AGCCAACCGG CTGAGTGCTG TCATTCAAGC CTTCTACATG
    TGCTGTTCCT GTCAGATGCC TCCAGGAATG ACTTCACCTC GTTTCATTGA GTTTGTCTGC
    AAGCATGATG AAGTATTGAA ATGCTTTGTG AATAGAAACC CCAAAATTAT ATTTGACCAT
    TTTCACTTTC TTCTTGAATG TCCTGAATTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCATTTA AAGATCGATG TGAGTGGTTC TATGAACATC TGCACTCAGG ACAGCCGGAC
    TCAGACATGG TCCACAGACC AGTGAGTGAG AACGATATCC TCCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AATTGTATCA AAAGCAAACT GTGCAAAGCT AAAGCAGGGG
    ATTGCTGTAC GATTTCATGG AGAAGAAGGC ATGGGTCAAG GCGTTGTGCG TGAATGGTTT
    GATATTCTGT CTAATGAGAT CGTCAACCCT GACTATGCAC TGTTCACCCA GTCAGCAGAC
    GGAACAACTT TCCAGCCCAA CAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC
    CGGTTTGCTG GACAGATCTT GGGGTTAGCT TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CATTCTACAA GCACATTCTC GGTATTCCTG TAAATTACCA AGATGTAGCA
    TCTATTGATC CTGAATATGC CAAAAATTTG CAATGGATTT TAGATAATGA TATTAGTGAC
    TTGGGTCTAG AATTGACTTT TTCTGTTGAG ACTGATGTAT TTGGAGCTAT GGAAGAGGTG
    CCTTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTATGTC
    CAGCTCGTTA CTGAACTGCG TATGACAAGA GCCATTCAGC CGCAGATCAA TGCGTTTTTA
    CAGGGTTTTC ATATGTTCAT CCCGCCTTCC CTCATACAAC TGTTTGACGA GTATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCGGAAATT GATGTAAATG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTGGAA
    GACATTACTC AAGAAGAAAG AGTTCTTCTC TTGCAGTTCG TTACTGGCAG TTCCAGGGTC
    CCGCATGGTG GCTTTGCCAA TATTATGGGT GGAAGTGGTT TGCAAAACTT TACAATTGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TCCTTGTAGC ACTGCATTGT
    GGCAGCTATG GTTATACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169354.1
    CDS772..2718
    Translation

    Target ORF information:

    RefSeq Version XM_017313865.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT TCGGGAAAAC ATGTTACGGC AAGTTCTGCA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATC CTGACAGGCC TTGCTGAAGT TGCTACGACA
    AATGGCCATA AGCTGCTTAG TTTATCTAGC AATTATGATG CGCAGATGAA GAGCCTTTTG
    AGGATTGTGA GGATATTTTG TCATGTCTTT CGAATTGGTC CCTCATCTCC CAGTAATGGA
    ATCGATATGG GCTACAATGG AAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTTTGGAA
    TTGCTTTGGC ACTCATTGGA TGAATGGTTA GTTTTAATAG CCACAGAATT AATGAAAAAC
    AAAGAGGATT CAACAGATAT TACTTCTATC TTGCTGAAAC AAAAAGGCCA GGATCAAGAG
    GCCCCTTCTC TTTCTGCATT TGAGCCACCA GGCCCTGGGA GCTATGAAAG CCTGCCCCCT
    GGTCCAGGGG ACTCCAAGCC AGAGGTGCTT GCAGGGGAGC AGGAAGCCAG TGCCGACTGT
    CAAGACGTCA TCTCTGTGAC AGCCAACCGG CTGAGTGCTG TCATTCAAGC CTTCTACATG
    TGCTGTTCCT GTCAGATGCC TCCAGGAATG ACTTCACCTC GTTTCATTGA GTTTGTCTGC
    AAGCATGATG AAGTATTGAA ATGCTTTGTG AATAGAAACC CCAAAATTAT ATTTGACCAT
    TTTCACTTTC TTCTTGAATG TCCTGAATTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCATTTA AAGATCGATG TGAGTGGTTC TATGAACATC TGCACTCAGG ACAGCCGGAC
    TCAGACATGG TCCACAGACC AGTGAGTGAG AACGATATCC TCCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AATTGTATCA AAAGCAAACT GTGCAAAGCT AAAGCAGGGG
    ATTGCTGTAC GATTTCATGG AGAAGAAGGC ATGGGTCAAG GCGTTGTGCG TGAATGGTTT
    GATATTCTGT CTAATGAGAT CGTCAACCCT GACTATGCAC TGTTCACCCA GTCAGCAGAC
    GGAACAACTT TCCAGCCCAA CAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC
    CGGTTTGCTG GACAGATCTT GGGGTTAGCT TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CATTCTACAA GCACATTCTC GGTATTCCTG TAAATTACCA AGATGTAGCA
    TCTATTGATC CTGAATATGC CAAAAATTTG CAATGGATTT TAGATAATGA TATTAGTGAC
    TTGGGTCTAG AATTGACTTT TTCTGTTGAG ACTGATGTAT TTGGAGCTAT GGAAGAGGTG
    CCTTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTATGTC
    CAGCTCGTTA CTGAACTGCG TATGACAAGA GCCATTCAGC CGCAGATCAA TGCGTTTTTA
    CAGGGTTTTC ATATGTTCAT CCCGCCTTCC CTCATACAAC TGTTTGACGA GTATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCGGAAATT GATGTAAATG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTGGAA
    GACATTACTC AAGAAGAAAG AGTTCTTCTC TTGCAGTTCG TTACTGGCAG TTCCAGGGTC
    CCGCATGGTG GCTTTGCCAA TATTATGGGT GGAAGTGGTT TGCAAAACTT TACAATTGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TCCTTGTAGC ACTGCATTGT
    GGCAGCTATG GTTATACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43797
    Clone ID Related Accession (Same CDS sequence) XM_006512634.4 , XM_006512634.3
    Accession Version XM_006512634.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTCGTTAC TGAACTGCGT
    ATGACAAGAG CCATTCAGCC GCAGATCAAT GCGTTTTTAC AGGGTTTTCA TATGTTCATC
    CCGCCTTCCC TCATACAACT GTTTGACGAG TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCGGAAATTG ATGTAAATGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ACATTACTCA AGAAGAAAGA
    GTTCTTCTCT TGCAGTTCGT TACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT
    ATTATGGGTG GAAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCCAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT CCTTGTAGCA CTGCATTGTG GCAGCTATGG TTATACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512697.1
    CDS499..3264
    Translation

    Target ORF information:

    RefSeq Version XM_006512634.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512634.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTCGTTAC TGAACTGCGT
    ATGACAAGAG CCATTCAGCC GCAGATCAAT GCGTTTTTAC AGGGTTTTCA TATGTTCATC
    CCGCCTTCCC TCATACAACT GTTTGACGAG TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCGGAAATTG ATGTAAATGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ACATTACTCA AGAAGAAAGA
    GTTCTTCTCT TGCAGTTCGT TACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT
    ATTATGGGTG GAAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCCAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT CCTTGTAGCA CTGCATTGTG GCAGCTATGG TTATACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43798
    Clone ID Related Accession (Same CDS sequence) XM_006512635.3 , XM_006512635.4
    Accession Version XM_006512635.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512635.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GCTGTGGATC TGTGGAATGC TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC
    CCTAACTATC AAGATATTTC AGGCTGTACA CCCCTTCATC TCGCTGCTAG AAATGGACAG
    AAGAAATGCA TGAGTAAATT ACTGGAGTAC AGTGCGGATG TCAACATTTG TAATAATGAA
    GGCCTTACAG CAATACACTG GCTGGCTGTG AATGGGAGGA CAGAGCTCCT CCATGACCTC
    GTGCAACACG TCACAGATGT GGATGTGGAG GATGCCATGG GGCAGACAGC GCTACACGTA
    GCCTGCCAGA ACGGGCACAA AACGACAGTG CAGTGCTTGC TAGACAGCGG TGCTGATATT
    AACAGGCCGA ATGTATCAGG AGCCACGCCA CTGTACTTTG CTTGCAGAAG AGTGTGTCTG
    ATTTTCTGGA CCTGGATTCA CAGTCATGGT CAGAGAGATA CTGCACAGAT TCTTCTATTA
    CGAGGAGCCA AATATTTACC AGATAAAAAT GGAGTAACCC CTCTAGATCT ATGTGTACAG
    GGTGGTTACG GACAGACTTG TGAAGTGTTA ATTCAGTACC ACCCGAGGCT TTTCCAGACT
    ATTGTTCAAA TGACACAGAA TGAAGACCTT CGGGAAAACA TGTTACGGCA AGTTCTGCAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATCC TGACAGGCCT TGCTGAAGTT
    GCTACGACAA ATGGCCATAA GCTGCTTAGT TTATCTAGCA ATTATGATGC GCAGATGAAG
    AGCCTTTTGA GGATTGTGAG GATATTTTGT CATGTCTTTC GAATTGGTCC CTCATCTCCC
    AGTAATGGAA TCGATATGGG CTACAATGGA AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTTTGGAAT TGCTTTGGCA CTCATTGGAT GAATGGTTAG TTTTAATAGC CACAGAATTA
    ATGAAAAACA AAGAGGATTC AACAGATATT ACTTCTATCT TGCTGAAACA AAAAGGCCAG
    GATCAAGAGG CCCCTTCTCT TTCTGCATTT GAGCCACCAG GCCCTGGGAG CTATGAAAGC
    CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT
    GCCGACTGTC AAGACGTCAT CTCTGTGACA GCCAACCGGC TGAGTGCTGT CATTCAAGCC
    TTCTACATGT GCTGTTCCTG TCAGATGCCT CCAGGAATGA CTTCACCTCG TTTCATTGAG
    TTTGTCTGCA AGCATGATGA AGTATTGAAA TGCTTTGTGA ATAGAAACCC CAAAATTATA
    TTTGACCATT TTCACTTTCT TCTTGAATGT CCTGAATTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCATTTAA AGATCGATGT GAGTGGTTCT ATGAACATCT GCACTCAGGA
    CAGCCGGACT CAGACATGGT CCACAGACCA GTGAGTGAGA ACGATATCCT CCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA ATTGTATCAA AAGCAAACTG TGCAAAGCTA
    AAGCAGGGGA TTGCTGTACG ATTTCATGGA GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT
    GAATGGTTTG ATATTCTGTC TAATGAGATC GTCAACCCTG ACTATGCACT GTTCACCCAG
    TCAGCAGACG GAACAACTTT CCAGCCCAAC AGCAACTCCT ATGTCAATCC TGATCACTTG
    AACTATTTCC GGTTTGCTGG ACAGATCTTG GGGTTAGCTT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC ATTCTACAAG CACATTCTCG GTATTCCTGT AAATTACCAA
    GATGTAGCAT CTATTGATCC TGAATATGCC AAAAATTTGC AATGGATTTT AGATAATGAT
    ATTAGTGACT TGGGTCTAGA ATTGACTTTT TCTGTTGAGA CTGATGTATT TGGAGCTATG
    GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA AGTATTCTTG TGACACAAAA TAACAAAGCG
    GAGTATGTCC AGCTCGTTAC TGAACTGCGT ATGACAAGAG CCATTCAGCC GCAGATCAAT
    GCGTTTTTAC AGGGTTTTCA TATGTTCATC CCGCCTTCCC TCATACAACT GTTTGACGAG
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCGGAAATTG ATGTAAATGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTGGAAG ACATTACTCA AGAAGAAAGA GTTCTTCTCT TGCAGTTCGT TACTGGCAGT
    TCCAGGGTCC CGCATGGTGG CTTTGCCAAT ATTATGGGTG GAAGTGGTTT GCAAAACTTT
    ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCCAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT CCTTGTAGCA
    CTGCATTGTG GCAGCTATGG TTATACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512698.1
    CDS500..3175
    Translation

    Target ORF information:

    RefSeq Version XM_006512635.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512635.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GCTGTGGATC TGTGGAATGC TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC
    CCTAACTATC AAGATATTTC AGGCTGTACA CCCCTTCATC TCGCTGCTAG AAATGGACAG
    AAGAAATGCA TGAGTAAATT ACTGGAGTAC AGTGCGGATG TCAACATTTG TAATAATGAA
    GGCCTTACAG CAATACACTG GCTGGCTGTG AATGGGAGGA CAGAGCTCCT CCATGACCTC
    GTGCAACACG TCACAGATGT GGATGTGGAG GATGCCATGG GGCAGACAGC GCTACACGTA
    GCCTGCCAGA ACGGGCACAA AACGACAGTG CAGTGCTTGC TAGACAGCGG TGCTGATATT
    AACAGGCCGA ATGTATCAGG AGCCACGCCA CTGTACTTTG CTTGCAGAAG AGTGTGTCTG
    ATTTTCTGGA CCTGGATTCA CAGTCATGGT CAGAGAGATA CTGCACAGAT TCTTCTATTA
    CGAGGAGCCA AATATTTACC AGATAAAAAT GGAGTAACCC CTCTAGATCT ATGTGTACAG
    GGTGGTTACG GACAGACTTG TGAAGTGTTA ATTCAGTACC ACCCGAGGCT TTTCCAGACT
    ATTGTTCAAA TGACACAGAA TGAAGACCTT CGGGAAAACA TGTTACGGCA AGTTCTGCAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATCC TGACAGGCCT TGCTGAAGTT
    GCTACGACAA ATGGCCATAA GCTGCTTAGT TTATCTAGCA ATTATGATGC GCAGATGAAG
    AGCCTTTTGA GGATTGTGAG GATATTTTGT CATGTCTTTC GAATTGGTCC CTCATCTCCC
    AGTAATGGAA TCGATATGGG CTACAATGGA AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTTTGGAAT TGCTTTGGCA CTCATTGGAT GAATGGTTAG TTTTAATAGC CACAGAATTA
    ATGAAAAACA AAGAGGATTC AACAGATATT ACTTCTATCT TGCTGAAACA AAAAGGCCAG
    GATCAAGAGG CCCCTTCTCT TTCTGCATTT GAGCCACCAG GCCCTGGGAG CTATGAAAGC
    CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT
    GCCGACTGTC AAGACGTCAT CTCTGTGACA GCCAACCGGC TGAGTGCTGT CATTCAAGCC
    TTCTACATGT GCTGTTCCTG TCAGATGCCT CCAGGAATGA CTTCACCTCG TTTCATTGAG
    TTTGTCTGCA AGCATGATGA AGTATTGAAA TGCTTTGTGA ATAGAAACCC CAAAATTATA
    TTTGACCATT TTCACTTTCT TCTTGAATGT CCTGAATTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCATTTAA AGATCGATGT GAGTGGTTCT ATGAACATCT GCACTCAGGA
    CAGCCGGACT CAGACATGGT CCACAGACCA GTGAGTGAGA ACGATATCCT CCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA ATTGTATCAA AAGCAAACTG TGCAAAGCTA
    AAGCAGGGGA TTGCTGTACG ATTTCATGGA GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT
    GAATGGTTTG ATATTCTGTC TAATGAGATC GTCAACCCTG ACTATGCACT GTTCACCCAG
    TCAGCAGACG GAACAACTTT CCAGCCCAAC AGCAACTCCT ATGTCAATCC TGATCACTTG
    AACTATTTCC GGTTTGCTGG ACAGATCTTG GGGTTAGCTT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC ATTCTACAAG CACATTCTCG GTATTCCTGT AAATTACCAA
    GATGTAGCAT CTATTGATCC TGAATATGCC AAAAATTTGC AATGGATTTT AGATAATGAT
    ATTAGTGACT TGGGTCTAGA ATTGACTTTT TCTGTTGAGA CTGATGTATT TGGAGCTATG
    GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA AGTATTCTTG TGACACAAAA TAACAAAGCG
    GAGTATGTCC AGCTCGTTAC TGAACTGCGT ATGACAAGAG CCATTCAGCC GCAGATCAAT
    GCGTTTTTAC AGGGTTTTCA TATGTTCATC CCGCCTTCCC TCATACAACT GTTTGACGAG
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCGGAAATTG ATGTAAATGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTGGAAG ACATTACTCA AGAAGAAAGA GTTCTTCTCT TGCAGTTCGT TACTGGCAGT
    TCCAGGGTCC CGCATGGTGG CTTTGCCAAT ATTATGGGTG GAAGTGGTTT GCAAAACTTT
    ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCCAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT CCTTGTAGCA
    CTGCATTGTG GCAGCTATGG TTATACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43799
    Clone ID Related Accession (Same CDS sequence) XM_006512636.3 , XM_006512636.4
    Accession Version XM_006512636.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512636.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCAATGG TGATGGCCGA CCAGCACAGG TCTGTTTCTG AACTCCTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAGAGAAGCT TGCTTCACAT TGCTGCAAAC
    TGTGGATCTG TGGAATGCTT GGTTCTTCTG TTGAAGAAAG GCGCAAACCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATCTC GCTGCTAGAA ATGGACAGAA GAAATGCATG
    AGTAAATTAC TGGAGTACAG TGCGGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACACTGGC TGGCTGTGAA TGGGAGGACA GAGCTCCTCC ATGACCTCGT GCAACACGTC
    ACAGATGTGG ATGTGGAGGA TGCCATGGGG CAGACAGCGC TACACGTAGC CTGCCAGAAC
    GGGCACAAAA CGACAGTGCA GTGCTTGCTA GACAGCGGTG CTGATATTAA CAGGCCGAAT
    GTATCAGGAG CCACGCCACT GTACTTTGCT TGCAGAAGAG TGTGTCTGAT TTTCTGGACC
    TGGATTCACA GTCATGGTCA GAGAGATACT GCACAGATTC TTCTATTACG AGGAGCCAAA
    TATTTACCAG ATAAAAATGG AGTAACCCCT CTAGATCTAT GTGTACAGGG TGGTTACGGA
    CAGACTTGTG AAGTGTTAAT TCAGTACCAC CCGAGGCTTT TCCAGACTAT TGTTCAAATG
    ACACAGAATG AAGACCTTCG GGAAAACATG TTACGGCAAG TTCTGCAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATCCTG ACAGGCCTTG CTGAAGTTGC TACGACAAAT
    GGCCATAAGC TGCTTAGTTT ATCTAGCAAT TATGATGCGC AGATGAAGAG CCTTTTGAGG
    ATTGTGAGGA TATTTTGTCA TGTCTTTCGA ATTGGTCCCT CATCTCCCAG TAATGGAATC
    GATATGGGCT ACAATGGAAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TTTGGAATTG
    CTTTGGCACT CATTGGATGA ATGGTTAGTT TTAATAGCCA CAGAATTAAT GAAAAACAAA
    GAGGATTCAA CAGATATTAC TTCTATCTTG CTGAAACAAA AAGGCCAGGA TCAAGAGGCC
    CCTTCTCTTT CTGCATTTGA GCCACCAGGC CCTGGGAGCT ATGAAAGCCT GCCCCCTGGT
    CCAGGGGACT CCAAGCCAGA GGTGCTTGCA GGGGAGCAGG AAGCCAGTGC CGACTGTCAA
    GACGTCATCT CTGTGACAGC CAACCGGCTG AGTGCTGTCA TTCAAGCCTT CTACATGTGC
    TGTTCCTGTC AGATGCCTCC AGGAATGACT TCACCTCGTT TCATTGAGTT TGTCTGCAAG
    CATGATGAAG TATTGAAATG CTTTGTGAAT AGAAACCCCA AAATTATATT TGACCATTTT
    CACTTTCTTC TTGAATGTCC TGAATTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCATTTAAAG ATCGATGTGA GTGGTTCTAT GAACATCTGC ACTCAGGACA GCCGGACTCA
    GACATGGTCC ACAGACCAGT GAGTGAGAAC GATATCCTCC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAAT TGTATCAAAA GCAAACTGTG CAAAGCTAAA GCAGGGGATT
    GCTGTACGAT TTCATGGAGA AGAAGGCATG GGTCAAGGCG TTGTGCGTGA ATGGTTTGAT
    ATTCTGTCTA ATGAGATCGT CAACCCTGAC TATGCACTGT TCACCCAGTC AGCAGACGGA
    ACAACTTTCC AGCCCAACAG CAACTCCTAT GTCAATCCTG ATCACTTGAA CTATTTCCGG
    TTTGCTGGAC AGATCTTGGG GTTAGCTTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCAT TCTACAAGCA CATTCTCGGT ATTCCTGTAA ATTACCAAGA TGTAGCATCT
    ATTGATCCTG AATATGCCAA AAATTTGCAA TGGATTTTAG ATAATGATAT TAGTGACTTG
    GGTCTAGAAT TGACTTTTTC TGTTGAGACT GATGTATTTG GAGCTATGGA AGAGGTGCCT
    TTAAAGCCTG GGGGTGGAAG TATTCTTGTG ACACAAAATA ACAAAGCGGA GTATGTCCAG
    CTCGTTACTG AACTGCGTAT GACAAGAGCC ATTCAGCCGC AGATCAATGC GTTTTTACAG
    GGTTTTCATA TGTTCATCCC GCCTTCCCTC ATACAACTGT TTGACGAGTA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC GGAAATTGAT GTAAATGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC
    ATTACTCAAG AAGAAAGAGT TCTTCTCTTG CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG
    CATGGTGGCT TTGCCAATAT TATGGGTGGA AGTGGTTTGC AAAACTTTAC AATTGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCCAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTCC TTGTAGCACT GCATTGTGGC
    AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512699.1
    CDS174..2837
    Translation

    Target ORF information:

    RefSeq Version XM_006512636.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512636.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCAATGG TGATGGCCGA CCAGCACAGG TCTGTTTCTG AACTCCTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAGAGAAGCT TGCTTCACAT TGCTGCAAAC
    TGTGGATCTG TGGAATGCTT GGTTCTTCTG TTGAAGAAAG GCGCAAACCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATCTC GCTGCTAGAA ATGGACAGAA GAAATGCATG
    AGTAAATTAC TGGAGTACAG TGCGGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACACTGGC TGGCTGTGAA TGGGAGGACA GAGCTCCTCC ATGACCTCGT GCAACACGTC
    ACAGATGTGG ATGTGGAGGA TGCCATGGGG CAGACAGCGC TACACGTAGC CTGCCAGAAC
    GGGCACAAAA CGACAGTGCA GTGCTTGCTA GACAGCGGTG CTGATATTAA CAGGCCGAAT
    GTATCAGGAG CCACGCCACT GTACTTTGCT TGCAGAAGAG TGTGTCTGAT TTTCTGGACC
    TGGATTCACA GTCATGGTCA GAGAGATACT GCACAGATTC TTCTATTACG AGGAGCCAAA
    TATTTACCAG ATAAAAATGG AGTAACCCCT CTAGATCTAT GTGTACAGGG TGGTTACGGA
    CAGACTTGTG AAGTGTTAAT TCAGTACCAC CCGAGGCTTT TCCAGACTAT TGTTCAAATG
    ACACAGAATG AAGACCTTCG GGAAAACATG TTACGGCAAG TTCTGCAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATCCTG ACAGGCCTTG CTGAAGTTGC TACGACAAAT
    GGCCATAAGC TGCTTAGTTT ATCTAGCAAT TATGATGCGC AGATGAAGAG CCTTTTGAGG
    ATTGTGAGGA TATTTTGTCA TGTCTTTCGA ATTGGTCCCT CATCTCCCAG TAATGGAATC
    GATATGGGCT ACAATGGAAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TTTGGAATTG
    CTTTGGCACT CATTGGATGA ATGGTTAGTT TTAATAGCCA CAGAATTAAT GAAAAACAAA
    GAGGATTCAA CAGATATTAC TTCTATCTTG CTGAAACAAA AAGGCCAGGA TCAAGAGGCC
    CCTTCTCTTT CTGCATTTGA GCCACCAGGC CCTGGGAGCT ATGAAAGCCT GCCCCCTGGT
    CCAGGGGACT CCAAGCCAGA GGTGCTTGCA GGGGAGCAGG AAGCCAGTGC CGACTGTCAA
    GACGTCATCT CTGTGACAGC CAACCGGCTG AGTGCTGTCA TTCAAGCCTT CTACATGTGC
    TGTTCCTGTC AGATGCCTCC AGGAATGACT TCACCTCGTT TCATTGAGTT TGTCTGCAAG
    CATGATGAAG TATTGAAATG CTTTGTGAAT AGAAACCCCA AAATTATATT TGACCATTTT
    CACTTTCTTC TTGAATGTCC TGAATTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCATTTAAAG ATCGATGTGA GTGGTTCTAT GAACATCTGC ACTCAGGACA GCCGGACTCA
    GACATGGTCC ACAGACCAGT GAGTGAGAAC GATATCCTCC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAAT TGTATCAAAA GCAAACTGTG CAAAGCTAAA GCAGGGGATT
    GCTGTACGAT TTCATGGAGA AGAAGGCATG GGTCAAGGCG TTGTGCGTGA ATGGTTTGAT
    ATTCTGTCTA ATGAGATCGT CAACCCTGAC TATGCACTGT TCACCCAGTC AGCAGACGGA
    ACAACTTTCC AGCCCAACAG CAACTCCTAT GTCAATCCTG ATCACTTGAA CTATTTCCGG
    TTTGCTGGAC AGATCTTGGG GTTAGCTTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCAT TCTACAAGCA CATTCTCGGT ATTCCTGTAA ATTACCAAGA TGTAGCATCT
    ATTGATCCTG AATATGCCAA AAATTTGCAA TGGATTTTAG ATAATGATAT TAGTGACTTG
    GGTCTAGAAT TGACTTTTTC TGTTGAGACT GATGTATTTG GAGCTATGGA AGAGGTGCCT
    TTAAAGCCTG GGGGTGGAAG TATTCTTGTG ACACAAAATA ACAAAGCGGA GTATGTCCAG
    CTCGTTACTG AACTGCGTAT GACAAGAGCC ATTCAGCCGC AGATCAATGC GTTTTTACAG
    GGTTTTCATA TGTTCATCCC GCCTTCCCTC ATACAACTGT TTGACGAGTA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC GGAAATTGAT GTAAATGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC
    ATTACTCAAG AAGAAAGAGT TCTTCTCTTG CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG
    CATGGTGGCT TTGCCAATAT TATGGGTGGA AGTGGTTTGC AAAACTTTAC AATTGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCCAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTCC TTGTAGCACT GCATTGTGGC
    AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43800
    Clone ID Related Accession (Same CDS sequence) XM_006512637.3 , XM_006512637.4
    Accession Version XM_006512637.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512637.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGAG CTACTGCTTT CTGGCATGCC GGAAATTGAT
    GTAAATGATT GGATAAAAAA TACAGAATAC ACAAGTGGCT ATGAAAGAGA AGATCCAGTT
    ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC ATTACTCAAG AAGAAAGAGT TCTTCTCTTG
    CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG CATGGTGGCT TTGCCAATAT TATGGGTGGA
    AGTGGTTTGC AAAACTTTAC AATTGCTGCT GTGCCATATA CTCCAAATCT TTTACCAACT
    TCCAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGTAAAGA AATACTCAAG
    GACAGACTCC TTGTAGCACT GCATTGTGGC AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512700.1
    CDS501..3134
    Translation

    Target ORF information:

    RefSeq Version XM_006512637.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512637.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGAG CTACTGCTTT CTGGCATGCC GGAAATTGAT
    GTAAATGATT GGATAAAAAA TACAGAATAC ACAAGTGGCT ATGAAAGAGA AGATCCAGTT
    ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC ATTACTCAAG AAGAAAGAGT TCTTCTCTTG
    CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG CATGGTGGCT TTGCCAATAT TATGGGTGGA
    AGTGGTTTGC AAAACTTTAC AATTGCTGCT GTGCCATATA CTCCAAATCT TTTACCAACT
    TCCAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGTAAAGA AATACTCAAG
    GACAGACTCC TTGTAGCACT GCATTGTGGC AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43801
    Clone ID Related Accession (Same CDS sequence) XM_006512638.3 , XM_006512638.4
    Accession Version XM_006512638.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512638.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGCCTTTG
    GAATTGCTTT GGCACTCATT GGATGAATGG TTAGTTTTAA TAGCCACAGA ATTAATGAAA
    AACAAAGAGG ATTCAACAGA TATTACTTCT ATCTTGCTGA AACAAAAAGG CCAGGATCAA
    GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA CCAGGCCCTG GGAGCTATGA AAGCCTGCCC
    CCTGGTCCAG GGGACTCCAA GCCAGAGGTG CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC
    TGTCAAGACG TCATCTCTGT GACAGCCAAC CGGCTGAGTG CTGTCATTCA AGCCTTCTAC
    ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA ATGACTTCAC CTCGTTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTATT GAAATGCTTT GTGAATAGAA ACCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG ATGTGAGTGG TTCTATGAAC ATCTGCACTC AGGACAGCCG
    GACTCAGACA TGGTCCACAG ACCAGTGAGT GAGAACGATA TCCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATTGTA TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG
    GGGATTGCTG TACGATTTCA TGGAGAAGAA GGCATGGGTC AAGGCGTTGT GCGTGAATGG
    TTTGATATTC TGTCTAATGA GATCGTCAAC CCTGACTATG CACTGTTCAC CCAGTCAGCA
    GACGGAACAA CTTTCCAGCC CAACAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGGTTA GCTTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCATTCTA CAAGCACATT CTCGGTATTC CTGTAAATTA CCAAGATGTA
    GCATCTATTG ATCCTGAATA TGCCAAAAAT TTGCAATGGA TTTTAGATAA TGATATTAGT
    GACTTGGGTC TAGAATTGAC TTTTTCTGTT GAGACTGATG TATTTGGAGC TATGGAAGAG
    GTGCCTTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTCG TTACTGAACT GCGTATGACA AGAGCCATTC AGCCGCAGAT CAATGCGTTT
    TTACAGGGTT TTCATATGTT CATCCCGCCT TCCCTCATAC AACTGTTTGA CGAGTATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCGGAA ATTGATGTAA ATGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGACATTA CTCAAGAAGA AAGAGTTCTT CTCTTGCAGT TCGTTACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATTATG GGTGGAAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTCCTTGT AGCACTGCAT
    TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512701.1
    CDS494..2683
    Translation

    Target ORF information:

    RefSeq Version XM_006512638.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512638.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGCCTTTG
    GAATTGCTTT GGCACTCATT GGATGAATGG TTAGTTTTAA TAGCCACAGA ATTAATGAAA
    AACAAAGAGG ATTCAACAGA TATTACTTCT ATCTTGCTGA AACAAAAAGG CCAGGATCAA
    GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA CCAGGCCCTG GGAGCTATGA AAGCCTGCCC
    CCTGGTCCAG GGGACTCCAA GCCAGAGGTG CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC
    TGTCAAGACG TCATCTCTGT GACAGCCAAC CGGCTGAGTG CTGTCATTCA AGCCTTCTAC
    ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA ATGACTTCAC CTCGTTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTATT GAAATGCTTT GTGAATAGAA ACCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG ATGTGAGTGG TTCTATGAAC ATCTGCACTC AGGACAGCCG
    GACTCAGACA TGGTCCACAG ACCAGTGAGT GAGAACGATA TCCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATTGTA TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG
    GGGATTGCTG TACGATTTCA TGGAGAAGAA GGCATGGGTC AAGGCGTTGT GCGTGAATGG
    TTTGATATTC TGTCTAATGA GATCGTCAAC CCTGACTATG CACTGTTCAC CCAGTCAGCA
    GACGGAACAA CTTTCCAGCC CAACAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGGTTA GCTTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCATTCTA CAAGCACATT CTCGGTATTC CTGTAAATTA CCAAGATGTA
    GCATCTATTG ATCCTGAATA TGCCAAAAAT TTGCAATGGA TTTTAGATAA TGATATTAGT
    GACTTGGGTC TAGAATTGAC TTTTTCTGTT GAGACTGATG TATTTGGAGC TATGGAAGAG
    GTGCCTTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTCG TTACTGAACT GCGTATGACA AGAGCCATTC AGCCGCAGAT CAATGCGTTT
    TTACAGGGTT TTCATATGTT CATCCCGCCT TCCCTCATAC AACTGTTTGA CGAGTATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCGGAA ATTGATGTAA ATGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGACATTA CTCAAGAAGA AAGAGTTCTT CTCTTGCAGT TCGTTACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATTATG GGTGGAAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTCCTTGT AGCACTGCAT
    TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu21882
    Clone ID Related Accession (Same CDS sequence) NM_172473.3
    Accession Version NM_172473.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2730bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC153847.7, AK042879.1 and AC135669.5. On Jan 7, 2010 this sequence version replaced NM_172473.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660817.149667.1, AK156958.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849375, SAMN00849380 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACTGCAC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAATGGAGTA ACCCCTCTAG ATCTATGTGT ACAGGGTGGT
    TACGGACAGA CTTGTGAAGT GTTAATTCAG TACCACCCGA GGCTTTTCCA GACTATTGTT
    CAAATGACAC AGAATGAAGA CCTTCGGGAA AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GCCTTGCTGA AGTTGCTACG
    ACAAATGGCC ATAAGCTGCT TAGTTTATCT AGCAATTATG ATGCGCAGAT GAAGAGCCTT
    TTGAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCCTCATC TCCCAGTAAT
    GGAATCGATA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTTTG
    GAATTGCTTT GGCACTCATT GGATGAATGG TTAGTTTTAA TAGCCACAGA ATTAATGAAA
    AACAAAGAGG ATTCAACAGA TATTACTTCT ATCTTGCTGA AACAAAAAGG CCAGGATCAA
    GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA CCAGGCCCTG GGAGCTATGA AAGCCTGCCC
    CCTGGTCCAG GGGACTCCAA GCCAGAGGTG CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC
    TGTCAAGACG TCATCTCTGT GACAGCCAAC CGGCTGAGTG CTGTCATTCA AGCCTTCTAC
    ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA ATGACTTCAC CTCGTTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTATT GAAATGCTTT GTGAATAGAA ACCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG ATGTGAGTGG TTCTATGAAC ATCTGCACTC AGGACAGCCG
    GACTCAGACA TGGTCCACAG ACCAGTGAGT GAGAACGATA TCCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATTGTA TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG
    GGGATTGCTG TACGATTTCA TGGAGAAGAA GGCATGGGTC AAGGCGTTGT GCGTGAATGG
    TTTGATATTC TGTCTAATGA GATCGTCAAC CCTGACTATG CACTGTTCAC CCAGTCAGCA
    GACGGAACAA CTTTCCAGCC CAACAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGGTTA GCTTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCATTCTA CAAGCACATT CTCGGTATTC CTGTAAATTA CCAAGATGTA
    GCATCTATTG ATCCTGAATA TGCCAAAAAT TTGCAATGGA TTTTAGATAA TGATATTAGT
    GACTTGGGTC TAGAATTGAC TTTTTCTGTT GAGACTGATG TATTTGGAGC TATGGAAGAG
    GTGCCTTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTCG TTACTGAACT GCGTATGACA AGAGCCATTC AGCCGCAGAT CAATGCGTTT
    TTACAGGGTT TTCATATGTT CATCCCGCCT TCCCTCATAC AACTGTTTGA CGAGTATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCGGAA ATTGATGTAA ATGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGACATTA CTCAAGAAGA AAGAGTTCTT CTCTTGCAGT TCGTTACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATTATG GGTGGAAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTCCTTGT AGCACTGCAT
    TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_766061.2
    CDS247..2976
    Translation

    Target ORF information:

    RefSeq Version NM_172473.3
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_172473.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGTCA TGGTCAGAGA GATACTGCAC AGATTCTTCT ATTACGAGGA
    GCCAAATATT TACCAGATAA AAATGGAGTA ACCCCTCTAG ATCTATGTGT ACAGGGTGGT
    TACGGACAGA CTTGTGAAGT GTTAATTCAG TACCACCCGA GGCTTTTCCA GACTATTGTT
    CAAATGACAC AGAATGAAGA CCTTCGGGAA AACATGTTAC GGCAAGTTCT GCAGCATTTG
    TCTCAGCAAA GTGAAAGCCA GTACCTAAAG ATCCTGACAG GCCTTGCTGA AGTTGCTACG
    ACAAATGGCC ATAAGCTGCT TAGTTTATCT AGCAATTATG ATGCGCAGAT GAAGAGCCTT
    TTGAGGATTG TGAGGATATT TTGTCATGTC TTTCGAATTG GTCCCTCATC TCCCAGTAAT
    GGAATCGATA TGGGCTACAA TGGAAATAAA ACTCCAAGAA GCCAGGTGTT CAAGCCTTTG
    GAATTGCTTT GGCACTCATT GGATGAATGG TTAGTTTTAA TAGCCACAGA ATTAATGAAA
    AACAAAGAGG ATTCAACAGA TATTACTTCT ATCTTGCTGA AACAAAAAGG CCAGGATCAA
    GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA CCAGGCCCTG GGAGCTATGA AAGCCTGCCC
    CCTGGTCCAG GGGACTCCAA GCCAGAGGTG CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC
    TGTCAAGACG TCATCTCTGT GACAGCCAAC CGGCTGAGTG CTGTCATTCA AGCCTTCTAC
    ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA ATGACTTCAC CTCGTTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTATT GAAATGCTTT GTGAATAGAA ACCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG ATGTGAGTGG TTCTATGAAC ATCTGCACTC AGGACAGCCG
    GACTCAGACA TGGTCCACAG ACCAGTGAGT GAGAACGATA TCCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATTGTA TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG
    GGGATTGCTG TACGATTTCA TGGAGAAGAA GGCATGGGTC AAGGCGTTGT GCGTGAATGG
    TTTGATATTC TGTCTAATGA GATCGTCAAC CCTGACTATG CACTGTTCAC CCAGTCAGCA
    GACGGAACAA CTTTCCAGCC CAACAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGGTTA GCTTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCATTCTA CAAGCACATT CTCGGTATTC CTGTAAATTA CCAAGATGTA
    GCATCTATTG ATCCTGAATA TGCCAAAAAT TTGCAATGGA TTTTAGATAA TGATATTAGT
    GACTTGGGTC TAGAATTGAC TTTTTCTGTT GAGACTGATG TATTTGGAGC TATGGAAGAG
    GTGCCTTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTCG TTACTGAACT GCGTATGACA AGAGCCATTC AGCCGCAGAT CAATGCGTTT
    TTACAGGGTT TTCATATGTT CATCCCGCCT TCCCTCATAC AACTGTTTGA CGAGTATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCGGAA ATTGATGTAA ATGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGACATTA CTCAAGAAGA AAGAGTTCTT CTCTTGCAGT TCGTTACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATTATG GGTGGAAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTCCTTGT AGCACTGCAT
    TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43797
    Clone ID Related Accession (Same CDS sequence) XM_006512634.4 , XM_006512634.3
    Accession Version XM_006512634.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512634.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTCGTTAC TGAACTGCGT
    ATGACAAGAG CCATTCAGCC GCAGATCAAT GCGTTTTTAC AGGGTTTTCA TATGTTCATC
    CCGCCTTCCC TCATACAACT GTTTGACGAG TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCGGAAATTG ATGTAAATGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ACATTACTCA AGAAGAAAGA
    GTTCTTCTCT TGCAGTTCGT TACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT
    ATTATGGGTG GAAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCCAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT CCTTGTAGCA CTGCATTGTG GCAGCTATGG TTATACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512697.1
    CDS261..3026
    Translation

    Target ORF information:

    RefSeq Version XM_006512634.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512634.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGCG GAGTATGTCC AGCTCGTTAC TGAACTGCGT
    ATGACAAGAG CCATTCAGCC GCAGATCAAT GCGTTTTTAC AGGGTTTTCA TATGTTCATC
    CCGCCTTCCC TCATACAACT GTTTGACGAG TATGAATTGG AGCTACTGCT TTCTGGCATG
    CCGGAAATTG ATGTAAATGA TTGGATAAAA AATACAGAAT ACACAAGTGG CTATGAAAGA
    GAAGATCCAG TTATTCAGTG GTTCTGGGAA GTTGTGGAAG ACATTACTCA AGAAGAAAGA
    GTTCTTCTCT TGCAGTTCGT TACTGGCAGT TCCAGGGTCC CGCATGGTGG CTTTGCCAAT
    ATTATGGGTG GAAGTGGTTT GCAAAACTTT ACAATTGCTG CTGTGCCATA TACTCCAAAT
    CTTTTACCAA CTTCCAGCAC ATGCATCAAC ATGCTCAAGT TACCTGAATA CCCAAGTAAA
    GAAATACTCA AGGACAGACT CCTTGTAGCA CTGCATTGTG GCAGCTATGG TTATACAATG
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43798
    Clone ID Related Accession (Same CDS sequence) XM_006512635.3 , XM_006512635.4
    Accession Version XM_006512635.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2676bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512635.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GCTGTGGATC TGTGGAATGC TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC
    CCTAACTATC AAGATATTTC AGGCTGTACA CCCCTTCATC TCGCTGCTAG AAATGGACAG
    AAGAAATGCA TGAGTAAATT ACTGGAGTAC AGTGCGGATG TCAACATTTG TAATAATGAA
    GGCCTTACAG CAATACACTG GCTGGCTGTG AATGGGAGGA CAGAGCTCCT CCATGACCTC
    GTGCAACACG TCACAGATGT GGATGTGGAG GATGCCATGG GGCAGACAGC GCTACACGTA
    GCCTGCCAGA ACGGGCACAA AACGACAGTG CAGTGCTTGC TAGACAGCGG TGCTGATATT
    AACAGGCCGA ATGTATCAGG AGCCACGCCA CTGTACTTTG CTTGCAGAAG AGTGTGTCTG
    ATTTTCTGGA CCTGGATTCA CAGTCATGGT CAGAGAGATA CTGCACAGAT TCTTCTATTA
    CGAGGAGCCA AATATTTACC AGATAAAAAT GGAGTAACCC CTCTAGATCT ATGTGTACAG
    GGTGGTTACG GACAGACTTG TGAAGTGTTA ATTCAGTACC ACCCGAGGCT TTTCCAGACT
    ATTGTTCAAA TGACACAGAA TGAAGACCTT CGGGAAAACA TGTTACGGCA AGTTCTGCAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATCC TGACAGGCCT TGCTGAAGTT
    GCTACGACAA ATGGCCATAA GCTGCTTAGT TTATCTAGCA ATTATGATGC GCAGATGAAG
    AGCCTTTTGA GGATTGTGAG GATATTTTGT CATGTCTTTC GAATTGGTCC CTCATCTCCC
    AGTAATGGAA TCGATATGGG CTACAATGGA AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTTTGGAAT TGCTTTGGCA CTCATTGGAT GAATGGTTAG TTTTAATAGC CACAGAATTA
    ATGAAAAACA AAGAGGATTC AACAGATATT ACTTCTATCT TGCTGAAACA AAAAGGCCAG
    GATCAAGAGG CCCCTTCTCT TTCTGCATTT GAGCCACCAG GCCCTGGGAG CTATGAAAGC
    CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT
    GCCGACTGTC AAGACGTCAT CTCTGTGACA GCCAACCGGC TGAGTGCTGT CATTCAAGCC
    TTCTACATGT GCTGTTCCTG TCAGATGCCT CCAGGAATGA CTTCACCTCG TTTCATTGAG
    TTTGTCTGCA AGCATGATGA AGTATTGAAA TGCTTTGTGA ATAGAAACCC CAAAATTATA
    TTTGACCATT TTCACTTTCT TCTTGAATGT CCTGAATTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCATTTAA AGATCGATGT GAGTGGTTCT ATGAACATCT GCACTCAGGA
    CAGCCGGACT CAGACATGGT CCACAGACCA GTGAGTGAGA ACGATATCCT CCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA ATTGTATCAA AAGCAAACTG TGCAAAGCTA
    AAGCAGGGGA TTGCTGTACG ATTTCATGGA GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT
    GAATGGTTTG ATATTCTGTC TAATGAGATC GTCAACCCTG ACTATGCACT GTTCACCCAG
    TCAGCAGACG GAACAACTTT CCAGCCCAAC AGCAACTCCT ATGTCAATCC TGATCACTTG
    AACTATTTCC GGTTTGCTGG ACAGATCTTG GGGTTAGCTT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC ATTCTACAAG CACATTCTCG GTATTCCTGT AAATTACCAA
    GATGTAGCAT CTATTGATCC TGAATATGCC AAAAATTTGC AATGGATTTT AGATAATGAT
    ATTAGTGACT TGGGTCTAGA ATTGACTTTT TCTGTTGAGA CTGATGTATT TGGAGCTATG
    GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA AGTATTCTTG TGACACAAAA TAACAAAGCG
    GAGTATGTCC AGCTCGTTAC TGAACTGCGT ATGACAAGAG CCATTCAGCC GCAGATCAAT
    GCGTTTTTAC AGGGTTTTCA TATGTTCATC CCGCCTTCCC TCATACAACT GTTTGACGAG
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCGGAAATTG ATGTAAATGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTGGAAG ACATTACTCA AGAAGAAAGA GTTCTTCTCT TGCAGTTCGT TACTGGCAGT
    TCCAGGGTCC CGCATGGTGG CTTTGCCAAT ATTATGGGTG GAAGTGGTTT GCAAAACTTT
    ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCCAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT CCTTGTAGCA
    CTGCATTGTG GCAGCTATGG TTATACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512698.1
    CDS261..2936
    Translation

    Target ORF information:

    RefSeq Version XM_006512635.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512635.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GCTGTGGATC TGTGGAATGC TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC
    CCTAACTATC AAGATATTTC AGGCTGTACA CCCCTTCATC TCGCTGCTAG AAATGGACAG
    AAGAAATGCA TGAGTAAATT ACTGGAGTAC AGTGCGGATG TCAACATTTG TAATAATGAA
    GGCCTTACAG CAATACACTG GCTGGCTGTG AATGGGAGGA CAGAGCTCCT CCATGACCTC
    GTGCAACACG TCACAGATGT GGATGTGGAG GATGCCATGG GGCAGACAGC GCTACACGTA
    GCCTGCCAGA ACGGGCACAA AACGACAGTG CAGTGCTTGC TAGACAGCGG TGCTGATATT
    AACAGGCCGA ATGTATCAGG AGCCACGCCA CTGTACTTTG CTTGCAGAAG AGTGTGTCTG
    ATTTTCTGGA CCTGGATTCA CAGTCATGGT CAGAGAGATA CTGCACAGAT TCTTCTATTA
    CGAGGAGCCA AATATTTACC AGATAAAAAT GGAGTAACCC CTCTAGATCT ATGTGTACAG
    GGTGGTTACG GACAGACTTG TGAAGTGTTA ATTCAGTACC ACCCGAGGCT TTTCCAGACT
    ATTGTTCAAA TGACACAGAA TGAAGACCTT CGGGAAAACA TGTTACGGCA AGTTCTGCAG
    CATTTGTCTC AGCAAAGTGA AAGCCAGTAC CTAAAGATCC TGACAGGCCT TGCTGAAGTT
    GCTACGACAA ATGGCCATAA GCTGCTTAGT TTATCTAGCA ATTATGATGC GCAGATGAAG
    AGCCTTTTGA GGATTGTGAG GATATTTTGT CATGTCTTTC GAATTGGTCC CTCATCTCCC
    AGTAATGGAA TCGATATGGG CTACAATGGA AATAAAACTC CAAGAAGCCA GGTGTTCAAG
    CCTTTGGAAT TGCTTTGGCA CTCATTGGAT GAATGGTTAG TTTTAATAGC CACAGAATTA
    ATGAAAAACA AAGAGGATTC AACAGATATT ACTTCTATCT TGCTGAAACA AAAAGGCCAG
    GATCAAGAGG CCCCTTCTCT TTCTGCATTT GAGCCACCAG GCCCTGGGAG CTATGAAAGC
    CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT
    GCCGACTGTC AAGACGTCAT CTCTGTGACA GCCAACCGGC TGAGTGCTGT CATTCAAGCC
    TTCTACATGT GCTGTTCCTG TCAGATGCCT CCAGGAATGA CTTCACCTCG TTTCATTGAG
    TTTGTCTGCA AGCATGATGA AGTATTGAAA TGCTTTGTGA ATAGAAACCC CAAAATTATA
    TTTGACCATT TTCACTTTCT TCTTGAATGT CCTGAATTGA TGTCAAGATT CATGCATATC
    ATAAAAGCAC AGCCATTTAA AGATCGATGT GAGTGGTTCT ATGAACATCT GCACTCAGGA
    CAGCCGGACT CAGACATGGT CCACAGACCA GTGAGTGAGA ACGATATCCT CCTGGTTCAC
    AGAGATTCTA TTTTTAGGAG TAGCTGTGAA ATTGTATCAA AAGCAAACTG TGCAAAGCTA
    AAGCAGGGGA TTGCTGTACG ATTTCATGGA GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT
    GAATGGTTTG ATATTCTGTC TAATGAGATC GTCAACCCTG ACTATGCACT GTTCACCCAG
    TCAGCAGACG GAACAACTTT CCAGCCCAAC AGCAACTCCT ATGTCAATCC TGATCACTTG
    AACTATTTCC GGTTTGCTGG ACAGATCTTG GGGTTAGCTT TGAACCACAG GCAGCTGGTC
    AATATTTACT TCACACGATC ATTCTACAAG CACATTCTCG GTATTCCTGT AAATTACCAA
    GATGTAGCAT CTATTGATCC TGAATATGCC AAAAATTTGC AATGGATTTT AGATAATGAT
    ATTAGTGACT TGGGTCTAGA ATTGACTTTT TCTGTTGAGA CTGATGTATT TGGAGCTATG
    GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA AGTATTCTTG TGACACAAAA TAACAAAGCG
    GAGTATGTCC AGCTCGTTAC TGAACTGCGT ATGACAAGAG CCATTCAGCC GCAGATCAAT
    GCGTTTTTAC AGGGTTTTCA TATGTTCATC CCGCCTTCCC TCATACAACT GTTTGACGAG
    TATGAATTGG AGCTACTGCT TTCTGGCATG CCGGAAATTG ATGTAAATGA TTGGATAAAA
    AATACAGAAT ACACAAGTGG CTATGAAAGA GAAGATCCAG TTATTCAGTG GTTCTGGGAA
    GTTGTGGAAG ACATTACTCA AGAAGAAAGA GTTCTTCTCT TGCAGTTCGT TACTGGCAGT
    TCCAGGGTCC CGCATGGTGG CTTTGCCAAT ATTATGGGTG GAAGTGGTTT GCAAAACTTT
    ACAATTGCTG CTGTGCCATA TACTCCAAAT CTTTTACCAA CTTCCAGCAC ATGCATCAAC
    ATGCTCAAGT TACCTGAATA CCCAAGTAAA GAAATACTCA AGGACAGACT CCTTGTAGCA
    CTGCATTGTG GCAGCTATGG TTATACAATG GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43799
    Clone ID Related Accession (Same CDS sequence) XM_006512636.3 , XM_006512636.4
    Accession Version XM_006512636.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2664bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512636.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCAATGG TGATGGCCGA CCAGCACAGG TCTGTTTCTG AACTCCTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAGAGAAGCT TGCTTCACAT TGCTGCAAAC
    TGTGGATCTG TGGAATGCTT GGTTCTTCTG TTGAAGAAAG GCGCAAACCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATCTC GCTGCTAGAA ATGGACAGAA GAAATGCATG
    AGTAAATTAC TGGAGTACAG TGCGGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACACTGGC TGGCTGTGAA TGGGAGGACA GAGCTCCTCC ATGACCTCGT GCAACACGTC
    ACAGATGTGG ATGTGGAGGA TGCCATGGGG CAGACAGCGC TACACGTAGC CTGCCAGAAC
    GGGCACAAAA CGACAGTGCA GTGCTTGCTA GACAGCGGTG CTGATATTAA CAGGCCGAAT
    GTATCAGGAG CCACGCCACT GTACTTTGCT TGCAGAAGAG TGTGTCTGAT TTTCTGGACC
    TGGATTCACA GTCATGGTCA GAGAGATACT GCACAGATTC TTCTATTACG AGGAGCCAAA
    TATTTACCAG ATAAAAATGG AGTAACCCCT CTAGATCTAT GTGTACAGGG TGGTTACGGA
    CAGACTTGTG AAGTGTTAAT TCAGTACCAC CCGAGGCTTT TCCAGACTAT TGTTCAAATG
    ACACAGAATG AAGACCTTCG GGAAAACATG TTACGGCAAG TTCTGCAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATCCTG ACAGGCCTTG CTGAAGTTGC TACGACAAAT
    GGCCATAAGC TGCTTAGTTT ATCTAGCAAT TATGATGCGC AGATGAAGAG CCTTTTGAGG
    ATTGTGAGGA TATTTTGTCA TGTCTTTCGA ATTGGTCCCT CATCTCCCAG TAATGGAATC
    GATATGGGCT ACAATGGAAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TTTGGAATTG
    CTTTGGCACT CATTGGATGA ATGGTTAGTT TTAATAGCCA CAGAATTAAT GAAAAACAAA
    GAGGATTCAA CAGATATTAC TTCTATCTTG CTGAAACAAA AAGGCCAGGA TCAAGAGGCC
    CCTTCTCTTT CTGCATTTGA GCCACCAGGC CCTGGGAGCT ATGAAAGCCT GCCCCCTGGT
    CCAGGGGACT CCAAGCCAGA GGTGCTTGCA GGGGAGCAGG AAGCCAGTGC CGACTGTCAA
    GACGTCATCT CTGTGACAGC CAACCGGCTG AGTGCTGTCA TTCAAGCCTT CTACATGTGC
    TGTTCCTGTC AGATGCCTCC AGGAATGACT TCACCTCGTT TCATTGAGTT TGTCTGCAAG
    CATGATGAAG TATTGAAATG CTTTGTGAAT AGAAACCCCA AAATTATATT TGACCATTTT
    CACTTTCTTC TTGAATGTCC TGAATTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCATTTAAAG ATCGATGTGA GTGGTTCTAT GAACATCTGC ACTCAGGACA GCCGGACTCA
    GACATGGTCC ACAGACCAGT GAGTGAGAAC GATATCCTCC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAAT TGTATCAAAA GCAAACTGTG CAAAGCTAAA GCAGGGGATT
    GCTGTACGAT TTCATGGAGA AGAAGGCATG GGTCAAGGCG TTGTGCGTGA ATGGTTTGAT
    ATTCTGTCTA ATGAGATCGT CAACCCTGAC TATGCACTGT TCACCCAGTC AGCAGACGGA
    ACAACTTTCC AGCCCAACAG CAACTCCTAT GTCAATCCTG ATCACTTGAA CTATTTCCGG
    TTTGCTGGAC AGATCTTGGG GTTAGCTTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCAT TCTACAAGCA CATTCTCGGT ATTCCTGTAA ATTACCAAGA TGTAGCATCT
    ATTGATCCTG AATATGCCAA AAATTTGCAA TGGATTTTAG ATAATGATAT TAGTGACTTG
    GGTCTAGAAT TGACTTTTTC TGTTGAGACT GATGTATTTG GAGCTATGGA AGAGGTGCCT
    TTAAAGCCTG GGGGTGGAAG TATTCTTGTG ACACAAAATA ACAAAGCGGA GTATGTCCAG
    CTCGTTACTG AACTGCGTAT GACAAGAGCC ATTCAGCCGC AGATCAATGC GTTTTTACAG
    GGTTTTCATA TGTTCATCCC GCCTTCCCTC ATACAACTGT TTGACGAGTA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC GGAAATTGAT GTAAATGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC
    ATTACTCAAG AAGAAAGAGT TCTTCTCTTG CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG
    CATGGTGGCT TTGCCAATAT TATGGGTGGA AGTGGTTTGC AAAACTTTAC AATTGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCCAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTCC TTGTAGCACT GCATTGTGGC
    AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512699.1
    CDS174..2837
    Translation

    Target ORF information:

    RefSeq Version XM_006512636.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512636.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGCCAATGG TGATGGCCGA CCAGCACAGG TCTGTTTCTG AACTCCTATC AAATTCAAAA 
    TTTGATGTCA ATTATGCATT CGGACGTGTG AAGAGAAGCT TGCTTCACAT TGCTGCAAAC
    TGTGGATCTG TGGAATGCTT GGTTCTTCTG TTGAAGAAAG GCGCAAACCC TAACTATCAA
    GATATTTCAG GCTGTACACC CCTTCATCTC GCTGCTAGAA ATGGACAGAA GAAATGCATG
    AGTAAATTAC TGGAGTACAG TGCGGATGTC AACATTTGTA ATAATGAAGG CCTTACAGCA
    ATACACTGGC TGGCTGTGAA TGGGAGGACA GAGCTCCTCC ATGACCTCGT GCAACACGTC
    ACAGATGTGG ATGTGGAGGA TGCCATGGGG CAGACAGCGC TACACGTAGC CTGCCAGAAC
    GGGCACAAAA CGACAGTGCA GTGCTTGCTA GACAGCGGTG CTGATATTAA CAGGCCGAAT
    GTATCAGGAG CCACGCCACT GTACTTTGCT TGCAGAAGAG TGTGTCTGAT TTTCTGGACC
    TGGATTCACA GTCATGGTCA GAGAGATACT GCACAGATTC TTCTATTACG AGGAGCCAAA
    TATTTACCAG ATAAAAATGG AGTAACCCCT CTAGATCTAT GTGTACAGGG TGGTTACGGA
    CAGACTTGTG AAGTGTTAAT TCAGTACCAC CCGAGGCTTT TCCAGACTAT TGTTCAAATG
    ACACAGAATG AAGACCTTCG GGAAAACATG TTACGGCAAG TTCTGCAGCA TTTGTCTCAG
    CAAAGTGAAA GCCAGTACCT AAAGATCCTG ACAGGCCTTG CTGAAGTTGC TACGACAAAT
    GGCCATAAGC TGCTTAGTTT ATCTAGCAAT TATGATGCGC AGATGAAGAG CCTTTTGAGG
    ATTGTGAGGA TATTTTGTCA TGTCTTTCGA ATTGGTCCCT CATCTCCCAG TAATGGAATC
    GATATGGGCT ACAATGGAAA TAAAACTCCA AGAAGCCAGG TGTTCAAGCC TTTGGAATTG
    CTTTGGCACT CATTGGATGA ATGGTTAGTT TTAATAGCCA CAGAATTAAT GAAAAACAAA
    GAGGATTCAA CAGATATTAC TTCTATCTTG CTGAAACAAA AAGGCCAGGA TCAAGAGGCC
    CCTTCTCTTT CTGCATTTGA GCCACCAGGC CCTGGGAGCT ATGAAAGCCT GCCCCCTGGT
    CCAGGGGACT CCAAGCCAGA GGTGCTTGCA GGGGAGCAGG AAGCCAGTGC CGACTGTCAA
    GACGTCATCT CTGTGACAGC CAACCGGCTG AGTGCTGTCA TTCAAGCCTT CTACATGTGC
    TGTTCCTGTC AGATGCCTCC AGGAATGACT TCACCTCGTT TCATTGAGTT TGTCTGCAAG
    CATGATGAAG TATTGAAATG CTTTGTGAAT AGAAACCCCA AAATTATATT TGACCATTTT
    CACTTTCTTC TTGAATGTCC TGAATTGATG TCAAGATTCA TGCATATCAT AAAAGCACAG
    CCATTTAAAG ATCGATGTGA GTGGTTCTAT GAACATCTGC ACTCAGGACA GCCGGACTCA
    GACATGGTCC ACAGACCAGT GAGTGAGAAC GATATCCTCC TGGTTCACAG AGATTCTATT
    TTTAGGAGTA GCTGTGAAAT TGTATCAAAA GCAAACTGTG CAAAGCTAAA GCAGGGGATT
    GCTGTACGAT TTCATGGAGA AGAAGGCATG GGTCAAGGCG TTGTGCGTGA ATGGTTTGAT
    ATTCTGTCTA ATGAGATCGT CAACCCTGAC TATGCACTGT TCACCCAGTC AGCAGACGGA
    ACAACTTTCC AGCCCAACAG CAACTCCTAT GTCAATCCTG ATCACTTGAA CTATTTCCGG
    TTTGCTGGAC AGATCTTGGG GTTAGCTTTG AACCACAGGC AGCTGGTCAA TATTTACTTC
    ACACGATCAT TCTACAAGCA CATTCTCGGT ATTCCTGTAA ATTACCAAGA TGTAGCATCT
    ATTGATCCTG AATATGCCAA AAATTTGCAA TGGATTTTAG ATAATGATAT TAGTGACTTG
    GGTCTAGAAT TGACTTTTTC TGTTGAGACT GATGTATTTG GAGCTATGGA AGAGGTGCCT
    TTAAAGCCTG GGGGTGGAAG TATTCTTGTG ACACAAAATA ACAAAGCGGA GTATGTCCAG
    CTCGTTACTG AACTGCGTAT GACAAGAGCC ATTCAGCCGC AGATCAATGC GTTTTTACAG
    GGTTTTCATA TGTTCATCCC GCCTTCCCTC ATACAACTGT TTGACGAGTA TGAATTGGAG
    CTACTGCTTT CTGGCATGCC GGAAATTGAT GTAAATGATT GGATAAAAAA TACAGAATAC
    ACAAGTGGCT ATGAAAGAGA AGATCCAGTT ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC
    ATTACTCAAG AAGAAAGAGT TCTTCTCTTG CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG
    CATGGTGGCT TTGCCAATAT TATGGGTGGA AGTGGTTTGC AAAACTTTAC AATTGCTGCT
    GTGCCATATA CTCCAAATCT TTTACCAACT TCCAGCACAT GCATCAACAT GCTCAAGTTA
    CCTGAATACC CAAGTAAAGA AATACTCAAG GACAGACTCC TTGTAGCACT GCATTGTGGC
    AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43800
    Clone ID Related Accession (Same CDS sequence) XM_006512637.3 , XM_006512637.4
    Accession Version XM_006512637.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2634bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512637.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGAG CTACTGCTTT CTGGCATGCC GGAAATTGAT
    GTAAATGATT GGATAAAAAA TACAGAATAC ACAAGTGGCT ATGAAAGAGA AGATCCAGTT
    ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC ATTACTCAAG AAGAAAGAGT TCTTCTCTTG
    CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG CATGGTGGCT TTGCCAATAT TATGGGTGGA
    AGTGGTTTGC AAAACTTTAC AATTGCTGCT GTGCCATATA CTCCAAATCT TTTACCAACT
    TCCAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGTAAAGA AATACTCAAG
    GACAGACTCC TTGTAGCACT GCATTGTGGC AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512700.1
    CDS261..2894
    Translation

    Target ORF information:

    RefSeq Version XM_006512637.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512637.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGACAGTG
    CAGTGCTTGC TAGACAGCGG TGCTGATATT AACAGGCCGA ATGTATCAGG AGCCACGCCA
    CTGTACTTTG CTTGCAGAAG AGTGTGTCTG ATTTTCTGGA CCTGGATTCA CAGTCATGGT
    CAGAGAGATA CTGCACAGAT TCTTCTATTA CGAGGAGCCA AATATTTACC AGATAAAAAT
    GGAGTAACCC CTCTAGATCT ATGTGTACAG GGTGGTTACG GACAGACTTG TGAAGTGTTA
    ATTCAGTACC ACCCGAGGCT TTTCCAGACT ATTGTTCAAA TGACACAGAA TGAAGACCTT
    CGGGAAAACA TGTTACGGCA AGTTCTGCAG CATTTGTCTC AGCAAAGTGA AAGCCAGTAC
    CTAAAGATCC TGACAGGCCT TGCTGAAGTT GCTACGACAA ATGGCCATAA GCTGCTTAGT
    TTATCTAGCA ATTATGATGC GCAGATGAAG AGCCTTTTGA GGATTGTGAG GATATTTTGT
    CATGTCTTTC GAATTGGTCC CTCATCTCCC AGTAATGGAA TCGATATGGG CTACAATGGA
    AATAAAACTC CAAGAAGCCA GGTGTTCAAG CCTTTGGAAT TGCTTTGGCA CTCATTGGAT
    GAATGGTTAG TTTTAATAGC CACAGAATTA ATGAAAAACA AAGAGGATTC AACAGATATT
    ACTTCTATCT TGCTGAAACA AAAAGGCCAG GATCAAGAGG CCCCTTCTCT TTCTGCATTT
    GAGCCACCAG GCCCTGGGAG CTATGAAAGC CTGCCCCCTG GTCCAGGGGA CTCCAAGCCA
    GAGGTGCTTG CAGGGGAGCA GGAAGCCAGT GCCGACTGTC AAGACGTCAT CTCTGTGACA
    GCCAACCGGC TGAGTGCTGT CATTCAAGCC TTCTACATGT GCTGTTCCTG TCAGATGCCT
    CCAGGAATGA CTTCACCTCG TTTCATTGAG TTTGTCTGCA AGCATGATGA AGTATTGAAA
    TGCTTTGTGA ATAGAAACCC CAAAATTATA TTTGACCATT TTCACTTTCT TCTTGAATGT
    CCTGAATTGA TGTCAAGATT CATGCATATC ATAAAAGCAC AGCCATTTAA AGATCGATGT
    GAGTGGTTCT ATGAACATCT GCACTCAGGA CAGCCGGACT CAGACATGGT CCACAGACCA
    GTGAGTGAGA ACGATATCCT CCTGGTTCAC AGAGATTCTA TTTTTAGGAG TAGCTGTGAA
    ATTGTATCAA AAGCAAACTG TGCAAAGCTA AAGCAGGGGA TTGCTGTACG ATTTCATGGA
    GAAGAAGGCA TGGGTCAAGG CGTTGTGCGT GAATGGTTTG ATATTCTGTC TAATGAGATC
    GTCAACCCTG ACTATGCACT GTTCACCCAG TCAGCAGACG GAACAACTTT CCAGCCCAAC
    AGCAACTCCT ATGTCAATCC TGATCACTTG AACTATTTCC GGTTTGCTGG ACAGATCTTG
    GGGTTAGCTT TGAACCACAG GCAGCTGGTC AATATTTACT TCACACGATC ATTCTACAAG
    CACATTCTCG GTATTCCTGT AAATTACCAA GATGTAGCAT CTATTGATCC TGAATATGCC
    AAAAATTTGC AATGGATTTT AGATAATGAT ATTAGTGACT TGGGTCTAGA ATTGACTTTT
    TCTGTTGAGA CTGATGTATT TGGAGCTATG GAAGAGGTGC CTTTAAAGCC TGGGGGTGGA
    AGTATTCTTG TGACACAAAA TAACAAAGAG CTACTGCTTT CTGGCATGCC GGAAATTGAT
    GTAAATGATT GGATAAAAAA TACAGAATAC ACAAGTGGCT ATGAAAGAGA AGATCCAGTT
    ATTCAGTGGT TCTGGGAAGT TGTGGAAGAC ATTACTCAAG AAGAAAGAGT TCTTCTCTTG
    CAGTTCGTTA CTGGCAGTTC CAGGGTCCCG CATGGTGGCT TTGCCAATAT TATGGGTGGA
    AGTGGTTTGC AAAACTTTAC AATTGCTGCT GTGCCATATA CTCCAAATCT TTTACCAACT
    TCCAGCACAT GCATCAACAT GCTCAAGTTA CCTGAATACC CAAGTAAAGA AATACTCAAG
    GACAGACTCC TTGTAGCACT GCATTGTGGC AGCTATGGTT ATACAATGGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu43801
    Clone ID Related Accession (Same CDS sequence) XM_006512638.3 , XM_006512638.4
    Accession Version XM_006512638.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512638.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGCCTTTG
    GAATTGCTTT GGCACTCATT GGATGAATGG TTAGTTTTAA TAGCCACAGA ATTAATGAAA
    AACAAAGAGG ATTCAACAGA TATTACTTCT ATCTTGCTGA AACAAAAAGG CCAGGATCAA
    GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA CCAGGCCCTG GGAGCTATGA AAGCCTGCCC
    CCTGGTCCAG GGGACTCCAA GCCAGAGGTG CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC
    TGTCAAGACG TCATCTCTGT GACAGCCAAC CGGCTGAGTG CTGTCATTCA AGCCTTCTAC
    ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA ATGACTTCAC CTCGTTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTATT GAAATGCTTT GTGAATAGAA ACCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG ATGTGAGTGG TTCTATGAAC ATCTGCACTC AGGACAGCCG
    GACTCAGACA TGGTCCACAG ACCAGTGAGT GAGAACGATA TCCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATTGTA TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG
    GGGATTGCTG TACGATTTCA TGGAGAAGAA GGCATGGGTC AAGGCGTTGT GCGTGAATGG
    TTTGATATTC TGTCTAATGA GATCGTCAAC CCTGACTATG CACTGTTCAC CCAGTCAGCA
    GACGGAACAA CTTTCCAGCC CAACAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGGTTA GCTTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCATTCTA CAAGCACATT CTCGGTATTC CTGTAAATTA CCAAGATGTA
    GCATCTATTG ATCCTGAATA TGCCAAAAAT TTGCAATGGA TTTTAGATAA TGATATTAGT
    GACTTGGGTC TAGAATTGAC TTTTTCTGTT GAGACTGATG TATTTGGAGC TATGGAAGAG
    GTGCCTTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTCG TTACTGAACT GCGTATGACA AGAGCCATTC AGCCGCAGAT CAATGCGTTT
    TTACAGGGTT TTCATATGTT CATCCCGCCT TCCCTCATAC AACTGTTTGA CGAGTATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCGGAA ATTGATGTAA ATGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGACATTA CTCAAGAAGA AAGAGTTCTT CTCTTGCAGT TCGTTACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATTATG GGTGGAAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTCCTTGT AGCACTGCAT
    TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006512701.1
    CDS261..2450
    Translation

    Target ORF information:

    RefSeq Version XM_006512638.4
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006512638.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GGTCTGTTTC TGAACTCCTA TCAAATTCAA AATTTGATGT CAATTATGCA
    TTCGGACGTG TGAAGAGAAG CTTGCTTCAC ATTGCTGCAA ACTGTGGATC TGTGGAATGC
    TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC CCTAACTATC AAGATATTTC AGGCTGTACA
    CCCCTTCATC TCGCTGCTAG AAATGGACAG AAGAAATGCA TGAGTAAATT ACTGGAGTAC
    AGTGCGGATG TCAACATTTG TAATAATGAA GGCCTTACAG CAATACACTG GCTGGCTGTG
    AATGGGAGGA CAGAGCTCCT CCATGACCTC GTGCAACACG TCACAGATGT GGATGTGGAG
    GATGCCATGG GGCAGACAGC GCTACACGTA GCCTGCCAGA ACGGGCACAA AACGCCTTTG
    GAATTGCTTT GGCACTCATT GGATGAATGG TTAGTTTTAA TAGCCACAGA ATTAATGAAA
    AACAAAGAGG ATTCAACAGA TATTACTTCT ATCTTGCTGA AACAAAAAGG CCAGGATCAA
    GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA CCAGGCCCTG GGAGCTATGA AAGCCTGCCC
    CCTGGTCCAG GGGACTCCAA GCCAGAGGTG CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC
    TGTCAAGACG TCATCTCTGT GACAGCCAAC CGGCTGAGTG CTGTCATTCA AGCCTTCTAC
    ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA ATGACTTCAC CTCGTTTCAT TGAGTTTGTC
    TGCAAGCATG ATGAAGTATT GAAATGCTTT GTGAATAGAA ACCCCAAAAT TATATTTGAC
    CATTTTCACT TTCTTCTTGA ATGTCCTGAA TTGATGTCAA GATTCATGCA TATCATAAAA
    GCACAGCCAT TTAAAGATCG ATGTGAGTGG TTCTATGAAC ATCTGCACTC AGGACAGCCG
    GACTCAGACA TGGTCCACAG ACCAGTGAGT GAGAACGATA TCCTCCTGGT TCACAGAGAT
    TCTATTTTTA GGAGTAGCTG TGAAATTGTA TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG
    GGGATTGCTG TACGATTTCA TGGAGAAGAA GGCATGGGTC AAGGCGTTGT GCGTGAATGG
    TTTGATATTC TGTCTAATGA GATCGTCAAC CCTGACTATG CACTGTTCAC CCAGTCAGCA
    GACGGAACAA CTTTCCAGCC CAACAGCAAC TCCTATGTCA ATCCTGATCA CTTGAACTAT
    TTCCGGTTTG CTGGACAGAT CTTGGGGTTA GCTTTGAACC ACAGGCAGCT GGTCAATATT
    TACTTCACAC GATCATTCTA CAAGCACATT CTCGGTATTC CTGTAAATTA CCAAGATGTA
    GCATCTATTG ATCCTGAATA TGCCAAAAAT TTGCAATGGA TTTTAGATAA TGATATTAGT
    GACTTGGGTC TAGAATTGAC TTTTTCTGTT GAGACTGATG TATTTGGAGC TATGGAAGAG
    GTGCCTTTAA AGCCTGGGGG TGGAAGTATT CTTGTGACAC AAAATAACAA AGCGGAGTAT
    GTCCAGCTCG TTACTGAACT GCGTATGACA AGAGCCATTC AGCCGCAGAT CAATGCGTTT
    TTACAGGGTT TTCATATGTT CATCCCGCCT TCCCTCATAC AACTGTTTGA CGAGTATGAA
    TTGGAGCTAC TGCTTTCTGG CATGCCGGAA ATTGATGTAA ATGATTGGAT AAAAAATACA
    GAATACACAA GTGGCTATGA AAGAGAAGAT CCAGTTATTC AGTGGTTCTG GGAAGTTGTG
    GAAGACATTA CTCAAGAAGA AAGAGTTCTT CTCTTGCAGT TCGTTACTGG CAGTTCCAGG
    GTCCCGCATG GTGGCTTTGC CAATATTATG GGTGGAAGTG GTTTGCAAAA CTTTACAATT
    GCTGCTGTGC CATATACTCC AAATCTTTTA CCAACTTCCA GCACATGCAT CAACATGCTC
    AAGTTACCTG AATACCCAAG TAAAGAAATA CTCAAGGACA GACTCCTTGT AGCACTGCAT
    TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72690
    Clone ID Related Accession (Same CDS sequence) XM_030244983.1
    Accession Version XM_030244983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGGCAGA CAGCGCTACA CGTAGCCTGC CAGAACGGGC ACAAAACGAC AGTGCAGTGC 
    TTGCTAGACA GCGGTGCTGA TATTAACAGG CCGAATGTAT CAGGAGCCAC GCCACTGTAC
    TTTGCTTGCA GAAGAGTGTG TCTGATTTTC TGGACCTGGA TTCACAGTCA TGGTCAGAGA
    GATACTGCAC AGATTCTTCT ATTACGAGGA GCCAAATATT TACCAGATAA AAATGGAGTA
    ACCCCTCTAG ATCTATGTGT ACAGGGTGGT TACGGACAGA CTTGTGAAGT GTTAATTCAG
    TACCACCCGA GGCTTTTCCA GACTATTGTT CAAATGACAC AGAATGAAGA CCTTCGGGAA
    AACATGTTAC GGCAAGTTCT GCAGCATTTG TCTCAGCAAA GTGAAAGCCA GTACCTAAAG
    ATCCTGACAG GCCTTGCTGA AGTTGCTACG ACAAATGGCC ATAAGCTGCT TAGTTTATCT
    AGCAATTATG ATGCGCAGAT GAAGAGCCTT TTGAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCCTCATC TCCCAGTAAT GGAATCGATA TGGGCTACAA TGGAAATAAA
    ACTCCAAGAA GCCAGGTGTT CAAGCCTTTG GAATTGCTTT GGCACTCATT GGATGAATGG
    TTAGTTTTAA TAGCCACAGA ATTAATGAAA AACAAAGAGG ATTCAACAGA TATTACTTCT
    ATCTTGCTGA AACAAAAAGG CCAGGATCAA GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA
    CCAGGCCCTG GGAGCTATGA AAGCCTGCCC CCTGGTCCAG GGGACTCCAA GCCAGAGGTG
    CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC TGTCAAGACG TCATCTCTGT GACAGCCAAC
    CGGCTGAGTG CTGTCATTCA AGCCTTCTAC ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA
    ATGACTTCAC CTCGTTTCAT TGAGTTTGTC TGCAAGCATG ATGAAGTATT GAAATGCTTT
    GTGAATAGAA ACCCCAAAAT TATATTTGAC CATTTTCACT TTCTTCTTGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAA GCACAGCCAT TTAAAGATCG ATGTGAGTGG
    TTCTATGAAC ATCTGCACTC AGGACAGCCG GACTCAGACA TGGTCCACAG ACCAGTGAGT
    GAGAACGATA TCCTCCTGGT TCACAGAGAT TCTATTTTTA GGAGTAGCTG TGAAATTGTA
    TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG GGGATTGCTG TACGATTTCA TGGAGAAGAA
    GGCATGGGTC AAGGCGTTGT GCGTGAATGG TTTGATATTC TGTCTAATGA GATCGTCAAC
    CCTGACTATG CACTGTTCAC CCAGTCAGCA GACGGAACAA CTTTCCAGCC CAACAGCAAC
    TCCTATGTCA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGGTTA
    GCTTTGAACC ACAGGCAGCT GGTCAATATT TACTTCACAC GATCATTCTA CAAGCACATT
    CTCGGTATTC CTGTAAATTA CCAAGATGTA GCATCTATTG ATCCTGAATA TGCCAAAAAT
    TTGCAATGGA TTTTAGATAA TGATATTAGT GACTTGGGTC TAGAATTGAC TTTTTCTGTT
    GAGACTGATG TATTTGGAGC TATGGAAGAG GTGCCTTTAA AGCCTGGGGG TGGAAGTATT
    CTTGTGACAC AAAATAACAA AGCGGAGTAT GTCCAGCTCG TTACTGAACT GCGTATGACA
    AGAGCCATTC AGCCGCAGAT CAATGCGTTT TTACAGGGTT TTCATATGTT CATCCCGCCT
    TCCCTCATAC AACTGTTTGA CGAGTATGAA TTGGAGCTAC TGCTTTCTGG CATGCCGGAA
    ATTGATGTAA ATGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGACATTA CTCAAGAAGA AAGAGTTCTT
    CTCTTGCAGT TCGTTACTGG CAGTTCCAGG GTCCCGCATG GTGGCTTTGC CAATATTATG
    GGTGGAAGTG GTTTGCAAAA CTTTACAATT GCTGCTGTGC CATATACTCC AAATCTTTTA
    CCAACTTCCA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG TAAAGAAATA
    CTCAAGGACA GACTCCTTGT AGCACTGCAT TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100843.1
    CDS228..2507
    Translation

    Target ORF information:

    RefSeq Version XM_030244983.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030244983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGGGCAGA CAGCGCTACA CGTAGCCTGC CAGAACGGGC ACAAAACGAC AGTGCAGTGC 
    TTGCTAGACA GCGGTGCTGA TATTAACAGG CCGAATGTAT CAGGAGCCAC GCCACTGTAC
    TTTGCTTGCA GAAGAGTGTG TCTGATTTTC TGGACCTGGA TTCACAGTCA TGGTCAGAGA
    GATACTGCAC AGATTCTTCT ATTACGAGGA GCCAAATATT TACCAGATAA AAATGGAGTA
    ACCCCTCTAG ATCTATGTGT ACAGGGTGGT TACGGACAGA CTTGTGAAGT GTTAATTCAG
    TACCACCCGA GGCTTTTCCA GACTATTGTT CAAATGACAC AGAATGAAGA CCTTCGGGAA
    AACATGTTAC GGCAAGTTCT GCAGCATTTG TCTCAGCAAA GTGAAAGCCA GTACCTAAAG
    ATCCTGACAG GCCTTGCTGA AGTTGCTACG ACAAATGGCC ATAAGCTGCT TAGTTTATCT
    AGCAATTATG ATGCGCAGAT GAAGAGCCTT TTGAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCCTCATC TCCCAGTAAT GGAATCGATA TGGGCTACAA TGGAAATAAA
    ACTCCAAGAA GCCAGGTGTT CAAGCCTTTG GAATTGCTTT GGCACTCATT GGATGAATGG
    TTAGTTTTAA TAGCCACAGA ATTAATGAAA AACAAAGAGG ATTCAACAGA TATTACTTCT
    ATCTTGCTGA AACAAAAAGG CCAGGATCAA GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA
    CCAGGCCCTG GGAGCTATGA AAGCCTGCCC CCTGGTCCAG GGGACTCCAA GCCAGAGGTG
    CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC TGTCAAGACG TCATCTCTGT GACAGCCAAC
    CGGCTGAGTG CTGTCATTCA AGCCTTCTAC ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA
    ATGACTTCAC CTCGTTTCAT TGAGTTTGTC TGCAAGCATG ATGAAGTATT GAAATGCTTT
    GTGAATAGAA ACCCCAAAAT TATATTTGAC CATTTTCACT TTCTTCTTGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAA GCACAGCCAT TTAAAGATCG ATGTGAGTGG
    TTCTATGAAC ATCTGCACTC AGGACAGCCG GACTCAGACA TGGTCCACAG ACCAGTGAGT
    GAGAACGATA TCCTCCTGGT TCACAGAGAT TCTATTTTTA GGAGTAGCTG TGAAATTGTA
    TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG GGGATTGCTG TACGATTTCA TGGAGAAGAA
    GGCATGGGTC AAGGCGTTGT GCGTGAATGG TTTGATATTC TGTCTAATGA GATCGTCAAC
    CCTGACTATG CACTGTTCAC CCAGTCAGCA GACGGAACAA CTTTCCAGCC CAACAGCAAC
    TCCTATGTCA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGGTTA
    GCTTTGAACC ACAGGCAGCT GGTCAATATT TACTTCACAC GATCATTCTA CAAGCACATT
    CTCGGTATTC CTGTAAATTA CCAAGATGTA GCATCTATTG ATCCTGAATA TGCCAAAAAT
    TTGCAATGGA TTTTAGATAA TGATATTAGT GACTTGGGTC TAGAATTGAC TTTTTCTGTT
    GAGACTGATG TATTTGGAGC TATGGAAGAG GTGCCTTTAA AGCCTGGGGG TGGAAGTATT
    CTTGTGACAC AAAATAACAA AGCGGAGTAT GTCCAGCTCG TTACTGAACT GCGTATGACA
    AGAGCCATTC AGCCGCAGAT CAATGCGTTT TTACAGGGTT TTCATATGTT CATCCCGCCT
    TCCCTCATAC AACTGTTTGA CGAGTATGAA TTGGAGCTAC TGCTTTCTGG CATGCCGGAA
    ATTGATGTAA ATGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGACATTA CTCAAGAAGA AAGAGTTCTT
    CTCTTGCAGT TCGTTACTGG CAGTTCCAGG GTCCCGCATG GTGGCTTTGC CAATATTATG
    GGTGGAAGTG GTTTGCAAAA CTTTACAATT GCTGCTGTGC CATATACTCC AAATCTTTTA
    CCAACTTCCA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG TAAAGAAATA
    CTCAAGGACA GACTCCTTGT AGCACTGCAT TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu72691
    Clone ID Related Accession (Same CDS sequence) XM_017313865.1 , XM_017313865.2
    Accession Version XM_017313865.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1947bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_017313865.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT TCGGGAAAAC ATGTTACGGC AAGTTCTGCA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATC CTGACAGGCC TTGCTGAAGT TGCTACGACA
    AATGGCCATA AGCTGCTTAG TTTATCTAGC AATTATGATG CGCAGATGAA GAGCCTTTTG
    AGGATTGTGA GGATATTTTG TCATGTCTTT CGAATTGGTC CCTCATCTCC CAGTAATGGA
    ATCGATATGG GCTACAATGG AAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTTTGGAA
    TTGCTTTGGC ACTCATTGGA TGAATGGTTA GTTTTAATAG CCACAGAATT AATGAAAAAC
    AAAGAGGATT CAACAGATAT TACTTCTATC TTGCTGAAAC AAAAAGGCCA GGATCAAGAG
    GCCCCTTCTC TTTCTGCATT TGAGCCACCA GGCCCTGGGA GCTATGAAAG CCTGCCCCCT
    GGTCCAGGGG ACTCCAAGCC AGAGGTGCTT GCAGGGGAGC AGGAAGCCAG TGCCGACTGT
    CAAGACGTCA TCTCTGTGAC AGCCAACCGG CTGAGTGCTG TCATTCAAGC CTTCTACATG
    TGCTGTTCCT GTCAGATGCC TCCAGGAATG ACTTCACCTC GTTTCATTGA GTTTGTCTGC
    AAGCATGATG AAGTATTGAA ATGCTTTGTG AATAGAAACC CCAAAATTAT ATTTGACCAT
    TTTCACTTTC TTCTTGAATG TCCTGAATTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCATTTA AAGATCGATG TGAGTGGTTC TATGAACATC TGCACTCAGG ACAGCCGGAC
    TCAGACATGG TCCACAGACC AGTGAGTGAG AACGATATCC TCCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AATTGTATCA AAAGCAAACT GTGCAAAGCT AAAGCAGGGG
    ATTGCTGTAC GATTTCATGG AGAAGAAGGC ATGGGTCAAG GCGTTGTGCG TGAATGGTTT
    GATATTCTGT CTAATGAGAT CGTCAACCCT GACTATGCAC TGTTCACCCA GTCAGCAGAC
    GGAACAACTT TCCAGCCCAA CAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC
    CGGTTTGCTG GACAGATCTT GGGGTTAGCT TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CATTCTACAA GCACATTCTC GGTATTCCTG TAAATTACCA AGATGTAGCA
    TCTATTGATC CTGAATATGC CAAAAATTTG CAATGGATTT TAGATAATGA TATTAGTGAC
    TTGGGTCTAG AATTGACTTT TTCTGTTGAG ACTGATGTAT TTGGAGCTAT GGAAGAGGTG
    CCTTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTATGTC
    CAGCTCGTTA CTGAACTGCG TATGACAAGA GCCATTCAGC CGCAGATCAA TGCGTTTTTA
    CAGGGTTTTC ATATGTTCAT CCCGCCTTCC CTCATACAAC TGTTTGACGA GTATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCGGAAATT GATGTAAATG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTGGAA
    GACATTACTC AAGAAGAAAG AGTTCTTCTC TTGCAGTTCG TTACTGGCAG TTCCAGGGTC
    CCGCATGGTG GCTTTGCCAA TATTATGGGT GGAAGTGGTT TGCAAAACTT TACAATTGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TCCTTGTAGC ACTGCATTGT
    GGCAGCTATG GTTATACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017169354.1
    CDS772..2718
    Translation

    Target ORF information:

    RefSeq Version XM_017313865.2
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017313865.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGACACAGA ATGAAGACCT TCGGGAAAAC ATGTTACGGC AAGTTCTGCA GCATTTGTCT 
    CAGCAAAGTG AAAGCCAGTA CCTAAAGATC CTGACAGGCC TTGCTGAAGT TGCTACGACA
    AATGGCCATA AGCTGCTTAG TTTATCTAGC AATTATGATG CGCAGATGAA GAGCCTTTTG
    AGGATTGTGA GGATATTTTG TCATGTCTTT CGAATTGGTC CCTCATCTCC CAGTAATGGA
    ATCGATATGG GCTACAATGG AAATAAAACT CCAAGAAGCC AGGTGTTCAA GCCTTTGGAA
    TTGCTTTGGC ACTCATTGGA TGAATGGTTA GTTTTAATAG CCACAGAATT AATGAAAAAC
    AAAGAGGATT CAACAGATAT TACTTCTATC TTGCTGAAAC AAAAAGGCCA GGATCAAGAG
    GCCCCTTCTC TTTCTGCATT TGAGCCACCA GGCCCTGGGA GCTATGAAAG CCTGCCCCCT
    GGTCCAGGGG ACTCCAAGCC AGAGGTGCTT GCAGGGGAGC AGGAAGCCAG TGCCGACTGT
    CAAGACGTCA TCTCTGTGAC AGCCAACCGG CTGAGTGCTG TCATTCAAGC CTTCTACATG
    TGCTGTTCCT GTCAGATGCC TCCAGGAATG ACTTCACCTC GTTTCATTGA GTTTGTCTGC
    AAGCATGATG AAGTATTGAA ATGCTTTGTG AATAGAAACC CCAAAATTAT ATTTGACCAT
    TTTCACTTTC TTCTTGAATG TCCTGAATTG ATGTCAAGAT TCATGCATAT CATAAAAGCA
    CAGCCATTTA AAGATCGATG TGAGTGGTTC TATGAACATC TGCACTCAGG ACAGCCGGAC
    TCAGACATGG TCCACAGACC AGTGAGTGAG AACGATATCC TCCTGGTTCA CAGAGATTCT
    ATTTTTAGGA GTAGCTGTGA AATTGTATCA AAAGCAAACT GTGCAAAGCT AAAGCAGGGG
    ATTGCTGTAC GATTTCATGG AGAAGAAGGC ATGGGTCAAG GCGTTGTGCG TGAATGGTTT
    GATATTCTGT CTAATGAGAT CGTCAACCCT GACTATGCAC TGTTCACCCA GTCAGCAGAC
    GGAACAACTT TCCAGCCCAA CAGCAACTCC TATGTCAATC CTGATCACTT GAACTATTTC
    CGGTTTGCTG GACAGATCTT GGGGTTAGCT TTGAACCACA GGCAGCTGGT CAATATTTAC
    TTCACACGAT CATTCTACAA GCACATTCTC GGTATTCCTG TAAATTACCA AGATGTAGCA
    TCTATTGATC CTGAATATGC CAAAAATTTG CAATGGATTT TAGATAATGA TATTAGTGAC
    TTGGGTCTAG AATTGACTTT TTCTGTTGAG ACTGATGTAT TTGGAGCTAT GGAAGAGGTG
    CCTTTAAAGC CTGGGGGTGG AAGTATTCTT GTGACACAAA ATAACAAAGC GGAGTATGTC
    CAGCTCGTTA CTGAACTGCG TATGACAAGA GCCATTCAGC CGCAGATCAA TGCGTTTTTA
    CAGGGTTTTC ATATGTTCAT CCCGCCTTCC CTCATACAAC TGTTTGACGA GTATGAATTG
    GAGCTACTGC TTTCTGGCAT GCCGGAAATT GATGTAAATG ATTGGATAAA AAATACAGAA
    TACACAAGTG GCTATGAAAG AGAAGATCCA GTTATTCAGT GGTTCTGGGA AGTTGTGGAA
    GACATTACTC AAGAAGAAAG AGTTCTTCTC TTGCAGTTCG TTACTGGCAG TTCCAGGGTC
    CCGCATGGTG GCTTTGCCAA TATTATGGGT GGAAGTGGTT TGCAAAACTT TACAATTGCT
    GCTGTGCCAT ATACTCCAAA TCTTTTACCA ACTTCCAGCA CATGCATCAA CATGCTCAAG
    TTACCTGAAT ACCCAAGTAA AGAAATACTC AAGGACAGAC TCCTTGTAGC ACTGCATTGT
    GGCAGCTATG GTTATACAAT GGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OMu85395
    Clone ID Related Accession (Same CDS sequence) XM_030244982.1
    Accession Version XM_030244982.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2640bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-07
    Organism Mus musculus(house mouse)
    Product E3 ubiquitin-protein ligase HACE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000076.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GCTGTGGATC TGTGGAATGC TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC
    CCTAACTATC AAGATATTTC AGGCTGTACA CCCCTTCATC TCGCTGCTAG AAATGGACAG
    AAGAAATGCA TGAGTAAATT ACTGGAGTAC AGTGCGGATG TCAACATTTG TAATAATGAA
    GGCCTTACAG CAATACACTG GCTGGCTGTG AATGGGAGGA CAGAGCTCCT CCATGACCTC
    GTGCAACACG TCACAGATGT GGATGTGGAG GATGCCATGG GGCAGACAGC GCTACACGTA
    GCCTGCCAGA ACGGGCACAA AACGACAGTG CAGTGCTTGC TAGACAGCGG TGCTGATATT
    AACAGGCCGA ATGTATCAGG AGCCACGCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GATACTGCAC AGATTCTTCT ATTACGAGGA GCCAAATATT TACCAGATAA AAATGGAGTA
    ACCCCTCTAG ATCTATGTGT ACAGGGTGGT TACGGACAGA CTTGTGAAGT GTTAATTCAG
    TACCACCCGA GGCTTTTCCA GACTATTGTT CAAATGACAC AGAATGAAGA CCTTCGGGAA
    AACATGTTAC GGCAAGTTCT GCAGCATTTG TCTCAGCAAA GTGAAAGCCA GTACCTAAAG
    ATCCTGACAG GCCTTGCTGA AGTTGCTACG ACAAATGGCC ATAAGCTGCT TAGTTTATCT
    AGCAATTATG ATGCGCAGAT GAAGAGCCTT TTGAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCCTCATC TCCCAGTAAT GGAATCGATA TGGGCTACAA TGGAAATAAA
    ACTCCAAGAA GCCAGGTGTT CAAGCCTTTG GAATTGCTTT GGCACTCATT GGATGAATGG
    TTAGTTTTAA TAGCCACAGA ATTAATGAAA AACAAAGAGG ATTCAACAGA TATTACTTCT
    ATCTTGCTGA AACAAAAAGG CCAGGATCAA GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA
    CCAGGCCCTG GGAGCTATGA AAGCCTGCCC CCTGGTCCAG GGGACTCCAA GCCAGAGGTG
    CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC TGTCAAGACG TCATCTCTGT GACAGCCAAC
    CGGCTGAGTG CTGTCATTCA AGCCTTCTAC ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA
    ATGACTTCAC CTCGTTTCAT TGAGTTTGTC TGCAAGCATG ATGAAGTATT GAAATGCTTT
    GTGAATAGAA ACCCCAAAAT TATATTTGAC CATTTTCACT TTCTTCTTGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAA GCACAGCCAT TTAAAGATCG ATGTGAGTGG
    TTCTATGAAC ATCTGCACTC AGGACAGCCG GACTCAGACA TGGTCCACAG ACCAGTGAGT
    GAGAACGATA TCCTCCTGGT TCACAGAGAT TCTATTTTTA GGAGTAGCTG TGAAATTGTA
    TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG GGGATTGCTG TACGATTTCA TGGAGAAGAA
    GGCATGGGTC AAGGCGTTGT GCGTGAATGG TTTGATATTC TGTCTAATGA GATCGTCAAC
    CCTGACTATG CACTGTTCAC CCAGTCAGCA GACGGAACAA CTTTCCAGCC CAACAGCAAC
    TCCTATGTCA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGGTTA
    GCTTTGAACC ACAGGCAGCT GGTCAATATT TACTTCACAC GATCATTCTA CAAGCACATT
    CTCGGTATTC CTGTAAATTA CCAAGATGTA GCATCTATTG ATCCTGAATA TGCCAAAAAT
    TTGCAATGGA TTTTAGATAA TGATATTAGT GACTTGGGTC TAGAATTGAC TTTTTCTGTT
    GAGACTGATG TATTTGGAGC TATGGAAGAG GTGCCTTTAA AGCCTGGGGG TGGAAGTATT
    CTTGTGACAC AAAATAACAA AGCGGAGTAT GTCCAGCTCG TTACTGAACT GCGTATGACA
    AGAGCCATTC AGCCGCAGAT CAATGCGTTT TTACAGGGTT TTCATATGTT CATCCCGCCT
    TCCCTCATAC AACTGTTTGA CGAGTATGAA TTGGAGCTAC TGCTTTCTGG CATGCCGGAA
    ATTGATGTAA ATGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGACATTA CTCAAGAAGA AAGAGTTCTT
    CTCTTGCAGT TCGTTACTGG CAGTTCCAGG GTCCCGCATG GTGGCTTTGC CAATATTATG
    GGTGGAAGTG GTTTGCAAAA CTTTACAATT GCTGCTGTGC CATATACTCC AAATCTTTTA
    CCAACTTCCA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG TAAAGAAATA
    CTCAAGGACA GACTCCTTGT AGCACTGCAT TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_030100842.1
    CDS261..2900
    Translation

    Target ORF information:

    RefSeq Version XM_030244982.1
    Organism Mus musculus(house mouse)
    Definition Mus musculus HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 (Hace1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_030244982.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGAGAGAG CGATGGAGCA GCTCAACCGT CTGACGCGCT CGCTGCGCCG CGCGCGCACC 
    GTGGAGCTGC CCGAGGATAA TGAGACTGCT GTTTACACAC TGATGCCAAT GGTGATGGCC
    GACCAGCACA GCTGTGGATC TGTGGAATGC TTGGTTCTTC TGTTGAAGAA AGGCGCAAAC
    CCTAACTATC AAGATATTTC AGGCTGTACA CCCCTTCATC TCGCTGCTAG AAATGGACAG
    AAGAAATGCA TGAGTAAATT ACTGGAGTAC AGTGCGGATG TCAACATTTG TAATAATGAA
    GGCCTTACAG CAATACACTG GCTGGCTGTG AATGGGAGGA CAGAGCTCCT CCATGACCTC
    GTGCAACACG TCACAGATGT GGATGTGGAG GATGCCATGG GGCAGACAGC GCTACACGTA
    GCCTGCCAGA ACGGGCACAA AACGACAGTG CAGTGCTTGC TAGACAGCGG TGCTGATATT
    AACAGGCCGA ATGTATCAGG AGCCACGCCA CTGTACTTTG CTTGCAGTCA TGGTCAGAGA
    GATACTGCAC AGATTCTTCT ATTACGAGGA GCCAAATATT TACCAGATAA AAATGGAGTA
    ACCCCTCTAG ATCTATGTGT ACAGGGTGGT TACGGACAGA CTTGTGAAGT GTTAATTCAG
    TACCACCCGA GGCTTTTCCA GACTATTGTT CAAATGACAC AGAATGAAGA CCTTCGGGAA
    AACATGTTAC GGCAAGTTCT GCAGCATTTG TCTCAGCAAA GTGAAAGCCA GTACCTAAAG
    ATCCTGACAG GCCTTGCTGA AGTTGCTACG ACAAATGGCC ATAAGCTGCT TAGTTTATCT
    AGCAATTATG ATGCGCAGAT GAAGAGCCTT TTGAGGATTG TGAGGATATT TTGTCATGTC
    TTTCGAATTG GTCCCTCATC TCCCAGTAAT GGAATCGATA TGGGCTACAA TGGAAATAAA
    ACTCCAAGAA GCCAGGTGTT CAAGCCTTTG GAATTGCTTT GGCACTCATT GGATGAATGG
    TTAGTTTTAA TAGCCACAGA ATTAATGAAA AACAAAGAGG ATTCAACAGA TATTACTTCT
    ATCTTGCTGA AACAAAAAGG CCAGGATCAA GAGGCCCCTT CTCTTTCTGC ATTTGAGCCA
    CCAGGCCCTG GGAGCTATGA AAGCCTGCCC CCTGGTCCAG GGGACTCCAA GCCAGAGGTG
    CTTGCAGGGG AGCAGGAAGC CAGTGCCGAC TGTCAAGACG TCATCTCTGT GACAGCCAAC
    CGGCTGAGTG CTGTCATTCA AGCCTTCTAC ATGTGCTGTT CCTGTCAGAT GCCTCCAGGA
    ATGACTTCAC CTCGTTTCAT TGAGTTTGTC TGCAAGCATG ATGAAGTATT GAAATGCTTT
    GTGAATAGAA ACCCCAAAAT TATATTTGAC CATTTTCACT TTCTTCTTGA ATGTCCTGAA
    TTGATGTCAA GATTCATGCA TATCATAAAA GCACAGCCAT TTAAAGATCG ATGTGAGTGG
    TTCTATGAAC ATCTGCACTC AGGACAGCCG GACTCAGACA TGGTCCACAG ACCAGTGAGT
    GAGAACGATA TCCTCCTGGT TCACAGAGAT TCTATTTTTA GGAGTAGCTG TGAAATTGTA
    TCAAAAGCAA ACTGTGCAAA GCTAAAGCAG GGGATTGCTG TACGATTTCA TGGAGAAGAA
    GGCATGGGTC AAGGCGTTGT GCGTGAATGG TTTGATATTC TGTCTAATGA GATCGTCAAC
    CCTGACTATG CACTGTTCAC CCAGTCAGCA GACGGAACAA CTTTCCAGCC CAACAGCAAC
    TCCTATGTCA ATCCTGATCA CTTGAACTAT TTCCGGTTTG CTGGACAGAT CTTGGGGTTA
    GCTTTGAACC ACAGGCAGCT GGTCAATATT TACTTCACAC GATCATTCTA CAAGCACATT
    CTCGGTATTC CTGTAAATTA CCAAGATGTA GCATCTATTG ATCCTGAATA TGCCAAAAAT
    TTGCAATGGA TTTTAGATAA TGATATTAGT GACTTGGGTC TAGAATTGAC TTTTTCTGTT
    GAGACTGATG TATTTGGAGC TATGGAAGAG GTGCCTTTAA AGCCTGGGGG TGGAAGTATT
    CTTGTGACAC AAAATAACAA AGCGGAGTAT GTCCAGCTCG TTACTGAACT GCGTATGACA
    AGAGCCATTC AGCCGCAGAT CAATGCGTTT TTACAGGGTT TTCATATGTT CATCCCGCCT
    TCCCTCATAC AACTGTTTGA CGAGTATGAA TTGGAGCTAC TGCTTTCTGG CATGCCGGAA
    ATTGATGTAA ATGATTGGAT AAAAAATACA GAATACACAA GTGGCTATGA AAGAGAAGAT
    CCAGTTATTC AGTGGTTCTG GGAAGTTGTG GAAGACATTA CTCAAGAAGA AAGAGTTCTT
    CTCTTGCAGT TCGTTACTGG CAGTTCCAGG GTCCCGCATG GTGGCTTTGC CAATATTATG
    GGTGGAAGTG GTTTGCAAAA CTTTACAATT GCTGCTGTGC CATATACTCC AAATCTTTTA
    CCAACTTCCA GCACATGCAT CAACATGCTC AAGTTACCTG AATACCCAAG TAAAGAAATA
    CTCAAGGACA GACTCCTTGT AGCACTGCAT TGTGGCAGCT ATGGTTATAC AATGGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    HACE1 is essential for astrocyte mitochondrial function and influences Huntington disease phenotypes in vivo.
    Human molecular genetics27(2)239-253(2018 Jan)
    Ehrnhoefer DE,Southwell AL,Sivasubramanian M,Qiu X,Villanueva EB,Xie Y,Waltl S,Anderson L,Fazeli A,Casal L,Felczak B,Tsang M,Hayden MR


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