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Nfs1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Summary

Gene Symbol Nfs1
Entrez Gene ID 84594
Full Name NFS1 cysteine desulfurase
Synonyms Nifs
General protein information
Preferred Names

NFS1 cysteine desulfurase

Names

cysteine desulfurase, mitochondrial
NFS1 nitrogen fixation 1 homolog
cysteine desulfurase, mitochondrial-like
nitrogen fixation gene 1
Gene Type protein-coding
Organism Rattus norvegicus(Norway rat)
Genome

3

3q42
Summary a putative cysteine desulfurase [RGD, Feb 2006].

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
NM_053462.2 NP_445914.2 cysteine desulfurase, mitochondrial
XM_006235365.2 XP_006235427.1 cysteine desulfurase, mitochondrial isoform X1

Pathways from BioSystems


Homologies


Homo sapiens (human) NFS1 NP_066923.3
Drosophila melanogaster (fruit fly) CG12264 NP_609533.1
Kluyveromyces lactis KLLA0D04928g XP_453279.1
Oryza sativa (rice) Os09g0338400 NP_001062914.1
Xenopus tropicalis (tropical clawed frog) nfs1 XP_002932937.2
Pan troglodytes (chimpanzee) NFS1 XP_001165456.1
NFS1 XP_001097983.1
Mus musculus (house mouse) Nfs1 NP_035041.2
Rattus norvegicus (Norway rat) Nfs1 NP_445914.2
Rattus norvegicus (Norway rat) LOC100911034 XP_003749657.1
Gallus gallus (chicken) NFS1 NP_001026018.1
Saccharomyces cerevisiae (baker's yeast) NFS1 NP_009912.2
Eremothecium gossypii AGOS_AAL013W NP_982529.1
Arabidopsis thaliana (thale cress) NFS1 NP_201373.1
Bos taurus (cattle) NFS1 NP_001092471.1
Gallus gallus (chicken) LOC100858210 XP_003642486.1
Caenorhabditis elegans (roundworm) B0205.6 NP_492812.2
Schizosaccharomyces pombe (fission yeast) nfs1 NP_596002.2
Magnaporthe oryzae (rice blast fungus) MGG_17371 XP_003718229.1
Neurospora crassa NCU04636 XP_959179.1
Canis lupus familiaris (dog) NFS1 XP_534405.2
Anopheles gambiae (African malaria mosquito) AgaP_AGAP009094 XP_319845.3
Danio rerio (zebrafish) nfs1 NP_001038458.1

Gene Ontology


Bibliography

Related articles in PubMed

Mitochondrial Hspa9/Mortalin regulates erythroid differentiation via iron-sulfur cluster assembly.
Shan Y, Cortopassi G
Mitochondrion2694-103(2016 Jan)

Iron-sulfur cluster biogenesis in mammalian cells: New insights into the molecular mechanisms of cluster delivery.
Maio N, Rouault TA
Biochimica et biophysica acta1853(6)1493-512(2015 Jun)

Human frataxin is an allosteric switch that activates the Fe-S cluster biosynthetic complex.
Tsai CL, Barondeau DP
Biochemistry49(43)9132-9(2010 Nov)

RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity both in mitochondria and cytosol of mammalian cells.
Fosset C, Chauveau MJ, Guillon B, Canal F, Drapier JC, Bouton C
The Journal of biological chemistry281(35)25398-406(2006 Sep)

Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation.
Biederbick A, Stehling O, R?sser R, Niggemeyer B, Nakai Y, Els?sser HP, Lill R
Molecular and cellular biology26(15)5675-87(2006 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Nfs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Nfs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa12481 NM_053462.2
Latest version!		 Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 5-7 $223.30
$319.00
ORa26573 XM_006235365.2
Latest version!		 Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID ORa12481
Clone ID Related Accession (Same CDS sequence) NM_053462.2
Accession Version NM_053462.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1380bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2019-12-24
Organism Rattus norvegicus(Norway rat)
Product cysteine desulfurase, mitochondrial
Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC104699.1. On Oct 4, 2005 this sequence version replaced NM_053462.1. ##Evidence-Data-START## Transcript exon combination :: BC104699.1, BC089205.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906349 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG CAGGTAGCGT CTCTGATTGG AGCGGATCCT 
CGGGAGATCA TTTTCACTAG TGGAGCTACG GAATCCAACA ACATAGCAAT TAAGGGAGTG 
GCCAGGTTCT ACAGGTCACG GAAAAAGCAC TTGGTCACCA CGCAGACAGA ACACAAGTGC 
GTGCTGGATT CGTGCCGCTC CCTGGAAGCT GAGGGCTTTC GGGTCACTTA CCTCCCCGTT 
CAGAAGAGTG GGATCATTGA CTTAAAGGAA CTAGAGGCTG CCATCCAGCC AGATACCAGC 
CTGGTCTCCG TTATGACTGT AAACAATGAG ATTGGAGTAA AGCAACCGAT TGCAGAAATA 
GGCCAGATTT GCAGTTCCAG AAAGCTGTAC TTCCATACTG ATGCAGCCCA AGCTGTTGGG 
AAAATCCCAC TTGATGTCAA TGACATGAAA ATTGATCTCA TGAGTATCAG TGGTCACAAA 
CTCTACGGCC CTAAAGGGGT TGGTGCTATC TATATCCGCC GTAGGCCCCG GGTGCGTGTG 
GAGGCCCTAC AAAGTGGAGG CGGGCAGGAG CGGGGTATGC GGTCTGGGAC AGTGCCCACA 
CCCTTGGTGG TGGGCCTGGG AGCTGCATGT GAGCTGGCAC AACAAGAGAT GGAGTACGAC 
CATAAACGGA TCTCAAAGTT AGCAGAGCGA CTCATACAGA AGATAATGAA GAACCTTCCA 
GATGTGGTGA TGAACGGGGA CCCGAAGCAG CACTACCCTG GCTGTATCAA CCTCTCCTTT 
GCGTATGTGG AAGGGGAAAG CCTGCTGATG GCACTCAAGG ACGTTGCCTT GTCCTCGGGG 
AGTGCCTGCA CTTCCGCATC ATTGGAGCCC TCGTATGTCC TCAGAGCCAT CGGTACGGAT 
GAGGATTTAG CACACTCCTC TATCAGGTTC GGCATCGGCC GCTTCACTAC AGAGGAGGAG 
GTAGACTACA CCGTGCAGAA GTGCATCCAC CATGTGAAAC GTCTTCGAGA AATGAGTCCT 
CTCTGGGAGA TGGTGCAGGA TGGCATTGAC CTCAAGAGCA TCAAGTGGAC CCAGCATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_445914.2
CDS16..1395
Translation

Target ORF information:

RefSeq Version NM_053462.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_053462.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG CAGGTAGCGT CTCTGATTGG AGCGGATCCT 
CGGGAGATCA TTTTCACTAG TGGAGCTACG GAATCCAACA ACATAGCAAT TAAGGGAGTG 
GCCAGGTTCT ACAGGTCACG GAAAAAGCAC TTGGTCACCA CGCAGACAGA ACACAAGTGC 
GTGCTGGATT CGTGCCGCTC CCTGGAAGCT GAGGGCTTTC GGGTCACTTA CCTCCCCGTT 
CAGAAGAGTG GGATCATTGA CTTAAAGGAA CTAGAGGCTG CCATCCAGCC AGATACCAGC 
CTGGTCTCCG TTATGACTGT AAACAATGAG ATTGGAGTAA AGCAACCGAT TGCAGAAATA 
GGCCAGATTT GCAGTTCCAG AAAGCTGTAC TTCCATACTG ATGCAGCCCA AGCTGTTGGG 
AAAATCCCAC TTGATGTCAA TGACATGAAA ATTGATCTCA TGAGTATCAG TGGTCACAAA 
CTCTACGGCC CTAAAGGGGT TGGTGCTATC TATATCCGCC GTAGGCCCCG GGTGCGTGTG 
GAGGCCCTAC AAAGTGGAGG CGGGCAGGAG CGGGGTATGC GGTCTGGGAC AGTGCCCACA 
CCCTTGGTGG TGGGCCTGGG AGCTGCATGT GAGCTGGCAC AACAAGAGAT GGAGTACGAC 
CATAAACGGA TCTCAAAGTT AGCAGAGCGA CTCATACAGA AGATAATGAA GAACCTTCCA 
GATGTGGTGA TGAACGGGGA CCCGAAGCAG CACTACCCTG GCTGTATCAA CCTCTCCTTT 
GCGTATGTGG AAGGGGAAAG CCTGCTGATG GCACTCAAGG ACGTTGCCTT GTCCTCGGGG 
AGTGCCTGCA CTTCCGCATC ATTGGAGCCC TCGTATGTCC TCAGAGCCAT CGGTACGGAT 
GAGGATTTAG CACACTCCTC TATCAGGTTC GGCATCGGCC GCTTCACTAC AGAGGAGGAG 
GTAGACTACA CCGTGCAGAA GTGCATCCAC CATGTGAAAC GTCTTCGAGA AATGAGTCCT 
CTCTGGGAGA TGGTGCAGGA TGGCATTGAC CTCAAGAGCA TCAAGTGGAC CCAGCATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID ORa26573
Clone ID Related Accession (Same CDS sequence) XM_006235365.2
Accession Version XM_006235365.2 Latest version! Documents for ORF clone product in default vector
Sequence Information ORF Nucleotide Sequence (Length: 1377bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ™ Seamless cloning technology
Insert Structure linear
Update Date 2016-07-25
Organism Rattus norvegicus(Norway rat)
Product cysteine desulfurase, mitochondrial isoform X1
Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006235365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG GTAGCGTCTC TGATTGGAGC GGATCCTCGG 
GAGATCATTT TCACTAGTGG AGCTACGGAA TCCAACAACA TAGCAATTAA GGGAGTGGCC 
AGGTTCTACA GGTCACGGAA AAAGCACTTG GTCACCACGC AGACAGAACA CAAGTGCGTG 
CTGGATTCGT GCCGCTCCCT GGAAGCTGAG GGCTTTCGGG TCACTTACCT CCCCGTTCAG 
AAGAGTGGGA TCATTGACTT AAAGGAACTA GAGGCTGCCA TCCAGCCAGA TACCAGCCTG 
GTCTCCGTTA TGACTGTAAA CAATGAGATT GGAGTAAAGC AACCGATTGC AGAAATAGGC 
CAGATTTGCA GTTCCAGAAA GCTGTACTTC CATACTGATG CAGCCCAAGC TGTTGGGAAA 
ATCCCACTTG ATGTCAATGA CATGAAAATT GATCTCATGA GTATCAGTGG TCACAAACTC 
TACGGCCCTA AAGGGGTTGG TGCTATCTAT ATCCGCCGTA GGCCCCGGGT GCGTGTGGAG 
GCCCTACAAA GTGGAGGCGG GCAGGAGCGG GGTATGCGGT CTGGGACAGT GCCCACACCC 
TTGGTGGTGG GCCTGGGAGC TGCATGTGAG CTGGCACAAC AAGAGATGGA GTACGACCAT 
AAACGGATCT CAAAGTTAGC AGAGCGACTC ATACAGAAGA TAATGAAGAA CCTTCCAGAT 
GTGGTGATGA ACGGGGACCC GAAGCAGCAC TACCCTGGCT GTATCAACCT CTCCTTTGCG 
TATGTGGAAG GGGAAAGCCT GCTGATGGCA CTCAAGGACG TTGCCTTGTC CTCGGGGAGT 
GCCTGCACTT CCGCATCATT GGAGCCCTCG TATGTCCTCA GAGCCATCGG TACGGATGAG 
GATTTAGCAC ACTCCTCTAT CAGGTTCGGC ATCGGCCGCT TCACTACAGA GGAGGAGGTA 
GACTACACCG TGCAGAAGTG CATCCACCAT GTGAAACGTC TTCGAGAAAT GAGTCCTCTC 
TGGGAGATGG TGCAGGATGG CATTGACCTC AAGAGCATCA AGTGGACCCA GCATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006235427.1
CDS18..1394
Translation

Target ORF information:

RefSeq Version XM_006235365.2
Organism Rattus norvegicus(Norway rat)
Definition Rattus norvegicus NFS1 cysteine desulfurase (Nfs1), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006235365.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
ATGGTGGGCT CTGTTGCTGG GAACATGCTG CTGCGAGCCG CTTGGAGGCG AGCGTCGTTG 
GCGGCTACCT CTGTGGCCCT GCGAAGGTCC TCGGTGCCCA CCCGGGGACT GCGCCTGCGC 
GTTGTAGATC ATGCCCCCCA TTCGGCTGTT CCCTCCGAGG CAGAGGCAGT GCTGCGACCT 
CTCTATATGG ACGTGCAGGC TACAACTCCT CTGGACCCCA GGGTGCTTGA TGCCATGCTC 
CCATACCTTG TCAACTACTA TGGGAACCCA CATTCTCGGA CTCATGCGTA TGGCTGGGAG 
AGCGAGGCAG CCATGGAACG TGCTCGCCAG GTAGCGTCTC TGATTGGAGC GGATCCTCGG 
GAGATCATTT TCACTAGTGG AGCTACGGAA TCCAACAACA TAGCAATTAA GGGAGTGGCC 
AGGTTCTACA GGTCACGGAA AAAGCACTTG GTCACCACGC AGACAGAACA CAAGTGCGTG 
CTGGATTCGT GCCGCTCCCT GGAAGCTGAG GGCTTTCGGG TCACTTACCT CCCCGTTCAG 
AAGAGTGGGA TCATTGACTT AAAGGAACTA GAGGCTGCCA TCCAGCCAGA TACCAGCCTG 
GTCTCCGTTA TGACTGTAAA CAATGAGATT GGAGTAAAGC AACCGATTGC AGAAATAGGC 
CAGATTTGCA GTTCCAGAAA GCTGTACTTC CATACTGATG CAGCCCAAGC TGTTGGGAAA 
ATCCCACTTG ATGTCAATGA CATGAAAATT GATCTCATGA GTATCAGTGG TCACAAACTC 
TACGGCCCTA AAGGGGTTGG TGCTATCTAT ATCCGCCGTA GGCCCCGGGT GCGTGTGGAG 
GCCCTACAAA GTGGAGGCGG GCAGGAGCGG GGTATGCGGT CTGGGACAGT GCCCACACCC 
TTGGTGGTGG GCCTGGGAGC TGCATGTGAG CTGGCACAAC AAGAGATGGA GTACGACCAT 
AAACGGATCT CAAAGTTAGC AGAGCGACTC ATACAGAAGA TAATGAAGAA CCTTCCAGAT 
GTGGTGATGA ACGGGGACCC GAAGCAGCAC TACCCTGGCT GTATCAACCT CTCCTTTGCG 
TATGTGGAAG GGGAAAGCCT GCTGATGGCA CTCAAGGACG TTGCCTTGTC CTCGGGGAGT 
GCCTGCACTT CCGCATCATT GGAGCCCTCG TATGTCCTCA GAGCCATCGG TACGGATGAG 
GATTTAGCAC ACTCCTCTAT CAGGTTCGGC ATCGGCCGCT TCACTACAGA GGAGGAGGTA 
GACTACACCG TGCAGAAGTG CATCCACCAT GTGAAACGTC TTCGAGAAAT GAGTCCTCTC 
TGGGAGATGG TGCAGGATGG CATTGACCTC AAGAGCATCA AGTGGACCCA GCATTAG

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

book

Mitochondrial Hspa9/Mortalin regulates erythroid differentiation via iron-sulfur cluster assembly.
Mitochondrion2694-103(2016 Jan)
Shan Y,Cortopassi G


book

Iron-sulfur cluster biogenesis in mammalian cells: New insights into the molecular mechanisms of cluster delivery.
Biochimica et biophysica acta1853(6)1493-512(2015 Jun)
Maio N,Rouault TA


book

Human frataxin is an allosteric switch that activates the Fe-S cluster biosynthetic complex.
Biochemistry49(43)9132-9(2010 Nov)
Tsai CL,Barondeau DP


book

RNA silencing of mitochondrial m-Nfs1 reduces Fe-S enzyme activity both in mitochondria and cytosol of mammalian cells.
The Journal of biological chemistry281(35)25398-406(2006 Sep)
Fosset C,Chauveau MJ,Guillon B,Canal F,Drapier JC,Bouton C


book

Role of human mitochondrial Nfs1 in cytosolic iron-sulfur protein biogenesis and iron regulation.
Molecular and cellular biology26(15)5675-87(2006 Aug)
Biederbick A,Stehling O,R?sser R,Niggemeyer B,Nakai Y,Els?sser HP,Lill R