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polyclonal antibody services quotation

Custom Polyclonal Antibody Services (Mouse)

These services generate custom mouse polyclonal antibodies using peptide antigens designed and optimized using our OptimunAntigen™ Design Tool.

Peptide Supplied Polyclonal Antibody Package (Mouse) (Cat. No.: SC1046)

Includes:
Delivery:
Time Frame:
1) Peptide synthesis: (<15 a.a, >85% purity, 7 mg) -50-100 μl of pre-immune serum 2-3 weeks
2) Conjugation: A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. -ELISA results 1 week
3) Immunization: Five Balb/c mice, pre-immune bleed, four immunizations/mouse-2.5 ml of antisera from all mice10 weeks
4) ELISA test -5 mg peptide, MS and HPLC report
5) Shipping 1-2 weeks
Total 16 weeks

Protein A-Purified Peptide Supplied Polyclonal Antibody Package (Mouse) (Cat. No.: SC1049)

polyclonal antibody services quotation
Includes:
Delivery:
Time Frame:
1) Peptide synthesis: (<15 a.a, >85% purity, 7 mg) -50-100 μl of pre-immune serum 2-3 weeks
2) Conjugation: A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. -ELISA results 1 week
3) Immunization: Five Balb/c mice, pre-immune bleed, four immunizations/mouse -5 mg peptide, MS and HPLC report 10 weeks
4) Antibody purification by protein A column -5-15 mg purified antibody from all antisera1 week
5) ELISA test
6) Shipping 1-2 weeks
Total 17 weeks

Standard Protocol (Five animals)

Steps Time from
Project Initiation
Time from Immunization Activity Description
Peptide synthesis
≈2 weeks
Day-14
-
Peptide conjugation to carrier
≈3 weeks
Day-7
-
Control serum collection
≈4 weeks
Day-1
Pre-bleed (≈0.1 ml serum)
Primary immunization
≈4 weeks
Day 0
50 μg per mouse peptide-KLH conjugate, Freud's Complete Adjuvant, Intrapreitoneal injection
1st boost
≈6 weeks
Day 14
25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection
Test bleed and ELISA
≈7 weeks
Day 21
0.01-0.02 ml serum per mouse. ELISA and response evaluation.
2nd boost
≈9 weeks
Day 35
25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection.
Test bleed and ELISA
≈10 weeks
Day 42
0.01-0.02 ml serum per mouse. ELISA and response evaluation.
3rd boost
≈12 weeks
Day 56
25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection
Production bleed
≈13 weeks
Day 63
0.5 ml serum per mouse
ELISA and shipping
≈15 weeks
Day 77
Extend or terminate

Note:

If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.

Quotations and Ordering

Ordering Process
polyclonal antibody services quotation
  • Request a quote and our professional account managers will contact you within 24 hours
  • If you need to submit your own physical antigen, mail it with the antigen submission form to: Antibody Services, GenScript, 860 Centennial Ave., Piscataway, NJ 08854, US.

Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.

 
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