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Custom Polyclonal Antibody Services (Rabbit)

These services generate custom rabbit polyclonal antibodies using peptide antigens designed and optimized using our OptimunAntigen™ Design Tool. All rabbit polyclonal antibody packages guarantee an ELISA titer of ≥1:64,000.

polyclonal antibody services quotation

Affinity-Purified Peptide Supplied Polyclonal Antibody
Package (Rabbit)
(Cat. No.: SC1031)

(Additional information: guarantees, advantages, key features etc.)

Includes:
Delivery:
Time Frame:
1) Peptide synthesis: (<15 a.a, >85% purity, 15 mg) 1-2 ml pre-immune serum 2-3 weeks
2) Conjugation: A cysteine is automatically applied to the C terminus (or N terminus) of the peptide and conjugated to KLH. ELISA titer results, ≥1:64,000 guaranteed 1 week
3) Immunization: Two rabbits, pre-immune bleed, three immunizations/rabbit 5 mg peptide, MS and HPLC reports 6 weeks
4) Final bleed
5) Peptide (5 mg) conjugated to affinity resin -10-50 mg affinity-purified antibodies 1 week
6) Antibody purification by column
7) ELISA test
8) Shipping 1-2 weeks
Total 13 weeks
polyclonal antibody services quotation

Peptide Supplied Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1015)

Includes:
Delivery:
Time Frame:
1) Peptide synthesis: (<15 a.a,>85% purity, 10 mg) 1-2 ml pre-immune serum 2-3 weeks
2) Conjugation: A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. ELISA titer results, ≥1:64,000 guaranteed 1 week
3) Immunization: Two rabbits, pre-immune bleed, four immunizations/rabbit 100-120 ml of antisera from two rabbits 10 weeks
4) Final bleed
5) ELISA -5 mg peptide, MS and HPLC reports
6) Shipping 1-2 weeks
Total 16 weeks
polyclonal antibody services quotation

Protein A-Purified Peptide Supplied Polyclonal Antibody Package (Rabbit)
(Cat. No.: SC1045)

Includes:
Delivery:
Time Frame:
1) Peptide synthesis: (<15 a.a, >85% purity, 10 mg) 1-2 ml pre-immune serum 2-3 weeks
2) Conjugation: A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. ELISA titer results, ≥1:64,000 guaranteed 1 week
3) Immunization: Two rabbits, pre-immune bleed, three immunizations/rabbit 5 mg peptide, MS and HPLC reports 7 weeks
4) Final bleed
5) Antibody purification by protein A column -200-500 mg protein A-purified antibodies 1 week
6) ELISA test
7) Shipping 1-2 weeks
Total 13 weeks
polyclonal antibody services quotation

Phospho-Specific Polyclonal Antibody Package (Rabbit) (Cat. No.: SC1044)

(Additional information: guarantees, advantages, key features etc.)

Includes:
Delivery:
Time Frame:
1) Peptide synthesis: synthesis of two peptides one with a modified amino acid (<15 a.a, >90% purity, 15 mg) and the other without modified amino acid (<15 a.a, >90% purity, 10 mg) 1-2 ml pre-immune serum 2-4 weeks
2) Conjugation: A cysteine is automatically added to the C terminus (or N terminus) of the peptide and conjugated to KLH. ELISA titer results, ≥1:64,000 guaranteed 1 week
3) Immunization: Two rabbits, pre-immune bleed, four immunizations/rabbit 5 mg phospho-peptide and 5 mg non-phospho peptide, MS and HPLC reports 10 weeks
4) Prepare two affinity columns 1-5 mg phospho-specific antibodies from two rabbits 1 week
5) Antibody purification by modified-peptide affinity column and unmodified-peptide cross-adsorption
6) ELISA test
7) Shipping 1-2 weeks
Total 17 weeks

* For collection and purification of antibodies from separated animals, a total of 2 ml of pre-immune serum (1 ml from each of the 2 animals) are provided. For antibody purification from pooled serum, 1 ml of pre-immune serum is provided.

Standard Protocol (Two animals)

More Details
Steps
Time from
Project Initiation
Time from Immunization
Activity Description
Peptide synthesis
≈2 weeks
Day -14
-
Peptide conjugation to carrier
≈3 weeks
Day -7
-
Control sera collection
≈4 weeks
Day -1
Pre-bleed (≈2-3 ml serum)
Primary immunization
≈4 weeks
Day 0
0.2 mg per rabbit peptide-KLH conjugate, Freud's Complete Adjuvant, Subcutaneous administration.
1st boost
≈6 weeks
Day 14
0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration
Test bleed and ELISA
≈7 weeks
Day 21
100 μl serum per rabbit. ELISA and immune response evaluation.
2nd boost
≈9 weeks
Day 35
0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration
Production bleed
≈10 weeks
Day 42
10-20 ml serum per rabbit
3rd boost
(SC1015 and SC1044 only)
≈12 weeks
Day 56
0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration
Production bleed
≈13 weeks
Day 63
40 ml serum per rabbit
ELISA and shipping
≈15weeks
Day 77
Extend or terminate

Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.

Quotations and Ordering

Ordering Process
polyclonal antibody services quotation
  • Request a quote and our professional account managers will contact you within 24 hours
  • If you need to submit your own physical antigen, mail it with the antigen submission form to: Antibody Services, GenScript, 860 Centennial Ave., Piscataway, NJ 08854, US.

Our customer service representatives are available 24 hours a day, Monday through Friday, to assist you.

 
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