Overview

GenScript's standard polyclonal antibody services packages use traditional immunization protocols and allow custom polyclonal antibody and antiserum production in rabbits, mice, and rats. Multiple antibody purification options are available and include: affinity purification, protein A/G and IgY purification or just antiserum. The availability of large animal species allows polyclonal antibody and antiserum production in large quantities and choice of animal host grants the use of unrelated animal species to ensure the specificity of your polyclonal antibody.

Highlights

Record Fast Turn-Around-Times

Record Fast
Turn-Around-Times

Higher Purified pAb Yields!

Higher Purified
pAb Yields!

Market High ELISA Titers of 1:256,000

Market High ELISA
Titers of 1:256,000

Advanced Antigen Design Tool

Advanced Antigen
Design Tool

Unsure which service line is appropriate for your application? Try our Antibody Service Selection Tool.

Service Details

Custom Rabbit Polyclonal Antibody Services
(SC2046)
Rabbit pAb
Custom Mouse Polyclonal Antibody Services
(SC2044)
Mouse pAb
Custom Rat Polyclonal Antibody Services
(SC2044)
Rat pAb

These services generate custom rabbit polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool. All rabbit polyclonal antibody packages guarantee an ELISA titer of ≥1:256,000.

Standard Protocol (Two animals)

Steps Time from Project Initiation Time from Immunization Activity Description
Peptide synthesis ≈2 weeks Day 21 -
Peptide conjugation to carrier ≈3 weeks Day 7 -
Primary immunization ≈4 weeks Day 0 0.2 mg per rabbit peptide-KLH conjugate, Freud's Complete Adjuvant, Subcutaneous administration
1st boost ≈6 weeks Day 14 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration
2nd boost ≈8 weeks Day 28 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration
Production bleed ≈9 weeks Day 35 10-20 ml serum per rabbit
3rd boost
(SC1044 only)
≈10 weeks Day 42 0.2 mg per rabbit peptide-KLH conjugate, Freud's Incomplete Adjuvant, Subcutaneous administration
Production bleed ≈11 weeks Day 49 40 ml serum per rabbit
ELISA, purification, and shipping ≈13 weeks Day 63 Extend or terminate

These services generate custom mouse polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool.

Standard Protocol (Five animals)

Steps Time from Project Initiation Time from Immunization Activity Description
Peptide synthesis ≈2 weeks Day 21 -
Peptide conjugation to carrier ≈3 weeks Day 7 -
Primary immunization ≈4 weeks Day 0 50 μg per mouse peptide-KLH conjugate, Freud's Complete Adjuvant, Intrapreitoneal injection.
1st boost ≈6 weeks Day 14 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection.
2nd boost ≈8 weeks Day 28 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection.
Test bleed and ELISA ≈9 weeks Day 35 0.01-0.02 ml serum per mouse. ELISA and response evaluation.
3rd boost ≈10 weeks Day 42 25 µg per mouse peptide-KLH conjugate, Freud's Incomplete Adjuvant, Intrapreitoneal injection.
Production bleed ≈11 weeks Day 49 0.5 ml serum per mouse.
ELISA, purification, and shipping ≈13 weeks Day 63 Extend or terminate

Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.
*During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.

These services generate custom rat polyclonal antibodies using peptide antigens designed and optimized using our OptimumAntigen Design Tool. Both rat antibody packages come with a guaranteed ELISA titer of ≥1:256,000.

Standard Protocol (Three animals)

Steps Time from Project Initiation Time from Immunization Activity Description
Peptide synthesis ≈2 weeks Day 21 -
Peptide conjugation to carrier ≈3 weeks Day 7 -
Primary immunization ≈4 weeks Day 0 50 µg per rat peptide-KLH conjugate, -Freunds's Complete Adjuvant, Intraperitoneal injection
1st boost ≈6 weeks Day 14 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection
2nd boost ≈8 weeks Day 28 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection
Test bleed and ELISA ≈9 weeks Day 35 0.01-0.02 ml serum per rat. ELISA and initial response evaluation.
3rd boost ≈10 weeks Day 42 25 µg per rat peptide-KLH conjugate, Freunds's Incomplete Adjuvant, Intraperitoneal injection
Production bleed ≈11 weeks Day 49 1.5 – 2 ml serum per rat
ELISA, purification, and shipping ≈13 weeks Day 63 Extend or terminate

Note: If no specific request, 0.02% w/v sodium azide will be added in the antisera and antibody products to prevent bacteria growth.
During the development of the antibody, if there is the death of the experimental animal, we will choose the adjusted immunization process for re-immunization to ensure the high quality and efficiency of the order.

Customer pAb Case Study

Learn how polyclonal antibodies maximize your chances of detecting novel target proteins

Learn how polyclonal antibodies maximize your chances of detecting novel target proteins

Get in Touch
with GenScript Custom
Antibody Services

Get in Touch with GenScript Custom Antibody Services
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Quotation and Ordering

Our customer service representatives are available 24 hours Monday through Friday. You may contact us anytime for assistance. Orders can be placed online, over the phone, by email or fax. Please have your payment information (credit card, purchase order or bank information) ready.

Fill out the online form and email to [email protected]
A Technical Account Manager will reply you within 24 hours to finalize the quote
To place order, provide credit card/PO information
Production of your project begins immediately
A Project Manager is in contact with you during the production process to give updates

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