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COL9A2 cDNA ORF clone, Homo sapiens (human)

Summary

Gene Symbol COL9A2
Entrez Gene ID 1298
Full Name collagen, type IX, alpha 2
Synonyms DJ39G22.4, EDM2, MED, STL5
General protein information
Preferred Names
collagen alpha-2(IX) chain
Names
collagen alpha-2(IX) chain
alpha 2 type IX collagen
collagen IX, alpha-2 polypeptide
Gene Type protein-coding
Organism Homo sapiens (human)
Genome

1

1p33-p32
Summary This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. This chain is unusual in that, unlike the other two type IX alpha chains, it contains a covalently attached glycosaminoglycan side chain. Mutations in this gene are associated with multiple epiphyseal dysplasia. [provided by RefSeq, Jul 2008]. lac of sum
Disorder MIM:

120260

Disorder Html: Epiphyseal dysplasia, multiple, 2, 600204 (3); {Intervertebral

Reference Sequences (RefSeq)

mRNA and Protein(s)

mRNA Protein Name
XM_011540714 XP_011539016 collagen alpha-2(IX) chain isoform X2
XM_006710365 XP_006710428 collagen alpha-2(IX) chain isoform X1
XM_011540715 XP_011539017 collagen alpha-2(IX) chain isoform X3
XM_011540716 XP_011539018 collagen alpha-2(IX) chain isoform X3
XM_011540717 XP_011539019 collagen alpha-2(IX) chain isoform X4
XM_011540718 XP_011539020 collagen alpha-2(IX) chain isoform X5
NM_001852 NP_001843 collagen alpha-2(IX) chain precursor

Pathways from BioSystems


Homologies


Homo sapiens (human) COL9A2 NP_001843.1
Pan troglodytes (chimpanzee) COL9A2 XP_003949434.1
Macaca mulatta (Rhesus monkey) COL9A2 XP_001082953.2
Canis lupus familiaris (dog) COL9A2 NP_001184099.1
Bos taurus (cattle) COL9A2 XP_002686588.2
Mus musculus (house mouse) Col9a2 NP_031767.2
Rattus norvegicus (Norway rat) Col9a2 NP_001102145.1
Gallus gallus (chicken) COL9A2 NP_001258917.1
Danio rerio (zebrafish) col9a2 NP_997744.1
Xenopus (Silurana) tropicalis (western clawed frog) col9a2 NP_001090661.1

Gene Ontology


Bibliography

Related articles in PubMed

  1. The NC2 domain of type IX collagen determines the chain register of the triple helix. Boudko SP, et al. J Biol Chem, 2012 Dec 28. PMID 23132862.
  2. A loss of function mutation in the COL9A2 gene causes autosomal recessive Stickler syndrome. Baker S, et al. Am J Med Genet A, 2011 Jul. PMID 21671392.
  3. Type IX collagen neo-deposition in degenerative discs of surgical patients whether genotyped plus or minus for COL9 risk alleles. Zhu Y, et al. Spine (Phila Pa 1976), 2011 Nov 15. PMID 21311409.
  4. A haplotype at the COL9A2 gene locus contributes to the genetic risk for lumbar spinal stenosis in the Korean population. Hyun SJ, et al. Spine (Phila Pa 1976), 2011 Jul 15. PMID 21228751.
  5. Candidate gene association study of magnetic resonance imaging-based hip osteoarthritis (OA): evidence for COL9A2 gene as a common predisposing factor for hip OA and lumbar disc degeneration. Näkki A, et al. J Rheumatol, 2011 Apr. PMID 21159828.

See all (47) citations in PubMed

See citations in PubMed for homologs of this gene provided by HomoloGene

GeneRIFs: Gene References Into Functions What's a GeneRIF?

  1. The NC2 domain of type IX collagen determines the chain composition but also the chain register of the triple helix.
    Title: The NC2 domain of type IX collagen determines the chain register of the triple helix.
  2. Studies indicate that two SNPs that introduce tryptophan polymorphisms in COL9A2 and COL9A3 are independently linked to an increased risk of lumbar disc disease.
    Title: Type IX collagen neo-deposition in degenerative discs of surgical patients whether genotyped plus or minus for COL9 risk alleles.
  3. Data indicate that no causal SNPs in COL9A2 were significantly associated with LSS, but Haplotype Analysis showed that the "GCAGCG" haplotype (HAP2) was overrepresented in LSS patients.
    Title: A haplotype at the COL9A2 gene locus contributes to the genetic risk for lumbar spinal stenosis in the Korean population.
  4. loss of function mutation in the COL9A2 gene causes autosomal recessive Stickler syndrome
    Title: A loss of function mutation in the COL9A2 gene causes autosomal recessive Stickler syndrome.
  5. Data show that the associating G allele in COL9A2 changes a glutamine to arginine or to tryptophan and may predispose to both hip osteoarthritis and lumbar disc degeneration, making it a candidate for degenerative connective tissue diseases.
    Title: Candidate gene association study of magnetic resonance imaging-based hip osteoarthritis (OA): evidence for COL9A2 gene as a common predisposing factor for hip OA and lumbar disc degeneration.
  6. Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
    Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.
  7. This study extends the range of gene-mutations that can cause multiple epiphyseal dysplasia-related myopathy.
    Title: Type IX collagen gene mutations can result in multiple epiphyseal dysplasia that is associated with osteochondritis dissecans and a mild myopathy.
  8. Observational study of gene-disease association and gene-gene interaction. (HuGE Navigator)
    Title: Genetic factors are associated with modic changes in endplates of lumbar vertebral bodies.
  9. Homozygosity for the Arg allele of Col9A2 seems to be more frequent in the patient group with early recurrence although the differences in the allele frequencies were statistically not significant.
    Title: Absence of the mutated Trp2 allele but a common polymorphism of the COL9A2 collagen gene is associated with early recurrence after lumbar discectomy in a German population.
  10. Observational study of genotype prevalence and gene-disease association. (HuGE Navigator)
    Title: Mutations in the known genes are not the major cause of MED; distinctive phenotypic entities among patients with no identified mutations.
Submit: New GeneRIF Correction See all (18)

The following COL9A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL9A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID RefSeq Accession Definition **Vector *Turnaround time Price Select
OHu56997 XM_011540714 PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu24811 XM_006710365 PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu56998 XM_011540715 PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu56998 XM_011540716 PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu56999 XM_011540717 PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu57000 XM_011540718 PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $223.30
$319.00
OHu24811 NM_001852 Homo sapiens collagen, type IX, alpha 2 (COL9A2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)


CloneID OHu56997
Clone ID Related Accession (Same CDS sequence) XM_011540714
Accession Version XM_011540714.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2082bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-2(IX) chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGG TGAGAGACGA GTGTCCAACC 
AACTGTCCAC CCGGAATGAA AGGTCCCCCA GGGCTGCAGG GAGTGAAGGG GCATGCGGGC 
AAACGCGGGA TTCTGGGTGA TCCTGGCCAC CAGGGGAAGC CGGGTCCCAA GGGAGATGTG 
GGTGCCTCTG GAGAGCAAGG CATCCCTGGA CCACCGGGTC CCCAGGGCAT CAGGGGCTAC 
CCAGGCATGG CAGGGCCCAA GGGAGAGACG GGCCCTCATG GATATAAAGG CATGGTGGGC 
GCTATCGGTG CCACTGGGCC ACCGGGTGAG GAAGGTCCTA GGGGACCGCC AGGCCGAGCT 
GGGGAGAAGG GTGACGAGGG CAGCCCAGGT ATTCGTGGAC CCCAGGGGAT CACAGGCCCG 
AAAGGAGCAA CGGGCCCCCC AGGCATCAAC GGCAAGGATG GGACCCCAGG CACGCCTGGC 
ATGAAGGGCA GTGCAGGACA GGCGGGACAG CCCGGAAGTC CAGGCCACCA GGGCCTAGCG 
GGTGTGCCAG GCCAGCCTGG GACAAAAGGA GGCCCTGGAG ACCAGGGTGA GCCGGGCCCG 
CAGGGCCTTC CTGGATTCTC TGGTCCCCCT GGGAAAGAGG GAGAGCCAGG GCCTCGAGGA 
GAAATTGGTC CCCAGGGCAT CATGGGACAG AAGGGTGACC AAGGCGAGAG GGGTCCAGTG 
GGGCAACCAG GCCCTCAGGG AAGGCAGGGC CCTAAGGGGG AGCAGGGCCC CCCCGGAATT 
CCAGGGCCCC AAGGCTTGCC AGGCGTCAAA GGAGACAAGG GCTCCCCAGG GAAGACCGGG 
CCCCGCGGCA AAGTGGGTGA CCCAGGGGTG GCCGGCCTCC CCGGAGAGAA AGGCGAGAAG 
GGCGAGTCCG GCGAGCCGGG GCCCAAGGGA CAGCAAGGAG TACGTGGAGA ACCCGGCTAC 
CCTGGCCCCA GCGGGGATGC GGGCGCCCCA GGGGTTCAGG GCTACCCTGG TCCCCCCGGC 
CCTCGAGGAC TGGCCGGGAA CCGAGGCGTG CCAGGACAGC CCGGGAGACA GGGCGTGGAG 
GGCCGGGATG CCACTGACCA GCACATCGTG GATGTGGCGC TGAAGATGCT GCAAGAGCAA 
CTGGCAGAGG TCGCCGTGAG TGCCAAGCGG GAAGCCCTGG GTGCGGTGGG CATGATGGGT 
CCTCCAGGAC CTCCTGGGCC CCCTGGGTAC CCAGGCAAGC AGGGCCCCCA TGGGCACCCT 
GGCCCTCGGG GCGTTCCTGG CATCGTGGGA GCCGTGGGTC AGATCGGCAA CACGGGGCCC 
AAGGGAAAAC GTGGAGAGAA GGGTGATCCA GGAGAAGTGG GACGGGGGCA CCCCGGGATG 
CCTGGGCCCC CAGGGATCCC AGGACTCCCT GGCCGGCCTG GCCAGGCAAT CAACGGCAAG 
GATGGAGATC GAGGGTCCCC AGGGGCTCCA GGAGAGGCAG GTCGACCTGG CCTGCCAGGC 
CCCGTGGGGC TGCCGGGCTT CTGTGAACCT GCCGCCTGCC TTGGAGCTTC GGCCTATGCC 
TCTGCCCGCC TTACAGAGCC TGGATCCATC AAGGGGCCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539016.1
CDS200..2281
Misc Feature(1)986..1159(+)
Misc Feature(2)1193..1369(+)
Misc Feature(3)1292..1453(+)
Misc Feature(4)1487..1660(+)
Translation

Target ORF information:

RefSeq Version XM_011540714
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011540714

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGG TGAGAGACGA GTGTCCAACC 
AACTGTCCAC CCGGAATGAA AGGTCCCCCA GGGCTGCAGG GAGTGAAGGG GCATGCGGGC 
AAACGCGGGA TTCTGGGTGA TCCTGGCCAC CAGGGGAAGC CGGGTCCCAA GGGAGATGTG 
GGTGCCTCTG GAGAGCAAGG CATCCCTGGA CCACCGGGTC CCCAGGGCAT CAGGGGCTAC 
CCAGGCATGG CAGGGCCCAA GGGAGAGACG GGCCCTCATG GATATAAAGG CATGGTGGGC 
GCTATCGGTG CCACTGGGCC ACCGGGTGAG GAAGGTCCTA GGGGACCGCC AGGCCGAGCT 
GGGGAGAAGG GTGACGAGGG CAGCCCAGGT ATTCGTGGAC CCCAGGGGAT CACAGGCCCG 
AAAGGAGCAA CGGGCCCCCC AGGCATCAAC GGCAAGGATG GGACCCCAGG CACGCCTGGC 
ATGAAGGGCA GTGCAGGACA GGCGGGACAG CCCGGAAGTC CAGGCCACCA GGGCCTAGCG 
GGTGTGCCAG GCCAGCCTGG GACAAAAGGA GGCCCTGGAG ACCAGGGTGA GCCGGGCCCG 
CAGGGCCTTC CTGGATTCTC TGGTCCCCCT GGGAAAGAGG GAGAGCCAGG GCCTCGAGGA 
GAAATTGGTC CCCAGGGCAT CATGGGACAG AAGGGTGACC AAGGCGAGAG GGGTCCAGTG 
GGGCAACCAG GCCCTCAGGG AAGGCAGGGC CCTAAGGGGG AGCAGGGCCC CCCCGGAATT 
CCAGGGCCCC AAGGCTTGCC AGGCGTCAAA GGAGACAAGG GCTCCCCAGG GAAGACCGGG 
CCCCGCGGCA AAGTGGGTGA CCCAGGGGTG GCCGGCCTCC CCGGAGAGAA AGGCGAGAAG 
GGCGAGTCCG GCGAGCCGGG GCCCAAGGGA CAGCAAGGAG TACGTGGAGA ACCCGGCTAC 
CCTGGCCCCA GCGGGGATGC GGGCGCCCCA GGGGTTCAGG GCTACCCTGG TCCCCCCGGC 
CCTCGAGGAC TGGCCGGGAA CCGAGGCGTG CCAGGACAGC CCGGGAGACA GGGCGTGGAG 
GGCCGGGATG CCACTGACCA GCACATCGTG GATGTGGCGC TGAAGATGCT GCAAGAGCAA 
CTGGCAGAGG TCGCCGTGAG TGCCAAGCGG GAAGCCCTGG GTGCGGTGGG CATGATGGGT 
CCTCCAGGAC CTCCTGGGCC CCCTGGGTAC CCAGGCAAGC AGGGCCCCCA TGGGCACCCT 
GGCCCTCGGG GCGTTCCTGG CATCGTGGGA GCCGTGGGTC AGATCGGCAA CACGGGGCCC 
AAGGGAAAAC GTGGAGAGAA GGGTGATCCA GGAGAAGTGG GACGGGGGCA CCCCGGGATG 
CCTGGGCCCC CAGGGATCCC AGGACTCCCT GGCCGGCCTG GCCAGGCAAT CAACGGCAAG 
GATGGAGATC GAGGGTCCCC AGGGGCTCCA GGAGAGGCAG GTCGACCTGG CCTGCCAGGC 
CCCGTGGGGC TGCCGGGCTT CTGTGAACCT GCCGCCTGCC TTGGAGCTTC GGCCTATGCC 
TCTGCCCGCC TTACAGAGCC TGGATCCATC AAGGGGCCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24811
Clone ID Related Accession (Same CDS sequence) NM_001852 , XM_006710365
Accession Version XM_006710365.2 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-2(IX) chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced gi:578798413. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC 
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT 
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA 
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA 
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC 
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT 
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG 
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT 
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC 
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT 
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC 
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC 
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA 
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA 
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC 
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC 
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG 
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC 
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC 
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT 
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC 
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT 
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA 
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA 
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG 
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT 
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006710428.1
CDS197..2266
Misc Feature(1)971..1144(+)
Misc Feature(2)1178..1354(+)
Misc Feature(3)1277..1438(+)
Misc Feature(4)1472..1645(+)
Translation

Target ORF information:

RefSeq Version XM_006710365
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_006710365

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC 
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT 
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA 
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA 
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC 
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT 
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG 
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT 
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC 
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT 
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC 
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC 
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA 
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA 
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC 
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC 
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG 
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC 
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC 
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT 
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC 
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT 
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA 
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA 
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG 
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT 
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56998
Clone ID Related Accession (Same CDS sequence) XM_011540715 , XM_011540716
Accession Version XM_011540715.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-2(IX) chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA 
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT 
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG 
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA 
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG 
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC 
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA 
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG 
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC 
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG 
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA 
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC 
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA 
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA 
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG 
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC 
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC 
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA 
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC 
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC 
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG 
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT 
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG 
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG 
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA 
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC 
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT 
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT 
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539017.1
CDS907..2706
Misc Feature(1)1411..1584(+)
Misc Feature(2)1618..1794(+)
Misc Feature(3)1717..1878(+)
Misc Feature(4)1912..2085(+)
Translation

Target ORF information:

RefSeq Version XM_011540715
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011540715

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA 
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT 
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG 
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA 
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG 
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC 
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA 
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG 
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC 
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG 
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA 
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC 
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA 
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA 
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG 
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC 
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC 
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA 
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC 
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC 
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG 
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT 
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG 
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG 
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA 
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC 
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT 
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT 
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56998
Clone ID Related Accession (Same CDS sequence) XM_011540715 , XM_011540716
Accession Version XM_011540716.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-2(IX) chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA 
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT 
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG 
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA 
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG 
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC 
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA 
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG 
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC 
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG 
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA 
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC 
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA 
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA 
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG 
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC 
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC 
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA 
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC 
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC 
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG 
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT 
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG 
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG 
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA 
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC 
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT 
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT 
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539018.1
CDS410..2209
Misc Feature(1)914..1087(+)
Misc Feature(2)1121..1297(+)
Misc Feature(3)1220..1381(+)
Misc Feature(4)1415..1588(+)
Translation

Target ORF information:

RefSeq Version XM_011540716
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011540716

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA 
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT 
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG 
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA 
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG 
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC 
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA 
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG 
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC 
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG 
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA 
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC 
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA 
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA 
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG 
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC 
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC 
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA 
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC 
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC 
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG 
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT 
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG 
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG 
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA 
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC 
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT 
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT 
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56999
Clone ID Related Accession (Same CDS sequence) XM_011540717
Accession Version XM_011540717.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-2(IX) chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAAGGTC CCCCAGGGCT GCAGGGAGTG AAGGGGCATG CGGGCAAACG CGGGATTCTG 
GGTGATCCTG GCCACCAGGG GAAGCCGGGT CCCAAGGGAG ATGTGGGTGC CTCTGGAGAG 
CAAGGCATCC CTGGACCACC GGGTCCCCAG GGCATCAGGG GCTACCCAGG CATGGCAGGG 
CCCAAGGGAG AGACGGGCCC TCATGGATAT AAAGGCATGG TGGGCGCTAT CGGTGCCACT 
GGGCCACCGG GTGAGGAAGG TCCTAGGGGA CCGCCAGGCC GAGCTGGGGA GAAGGGTGAC 
GAGGGCAGCC CAGGTATTCG TGGACCCCAG GGGATCACAG GCCCGAAAGG AGCAACGGGC 
CCCCCAGGCA TCAACGGCAA GGATGGGACC CCAGGCACGC CTGGCATGAA GGGCAGTGCA 
GGACAGGCGG GACAGCCCGG AAGTCCAGGC CACCAGGGCC TAGCGGGTGT GCCAGGCCAG 
CCTGGGACAA AAGGAGGCCC TGGAGACCAG GGTGAGCCGG GCCCGCAGGG CCTTCCTGGA 
TTCTCTGGTC CCCCTGGGAA AGAGGGAGAG CCAGGGCCTC GAGGAGAAAT TGGTCCCCAG 
GGCATCATGG GACAGAAGGG TGACCAAGGC GAGAGGGGTC CAGTGGGGCA ACCAGGCCCT 
CAGGGAAGGC AGGGCCCTAA GGGGGAGCAG GGCCCCCCCG GAATTCCAGG GCCCCAAGGC 
TTGCCAGGCG TCAAAGGAGA CAAGGGCTCC CCAGGGAAGA CCGGGCCCCG CGGCAAAGTG 
GGTGACCCAG GGGTGGCCGG CCTCCCCGGA GAGAAAGGCG AGAAGGGCGA GTCCGGCGAG 
CCGGGGCCCA AGGGACAGCA AGGAGTACGT GGAGAACCCG GCTACCCTGG CCCCAGCGGG 
GATGCGGGCG CCCCAGGGGT TCAGGGCTAC CCTGGTCCCC CCGGCCCTCG AGGACTGGCC 
GGGAACCGAG GCGTGCCAGG ACAGCCCGGG AGACAGGGCG TGGAGGGCCG GGATGCCACT 
GACCAGCACA TCGTGGATGT GGCGCTGAAG ATGCTGCAAG AGCAACTGGC AGAGGTCGCC 
GTGAGTGCCA AGCGGGAAGC CCTGGGTGCG GTGGGCATGA TGGGTCCTCC AGGACCTCCT 
GGGCCCCCTG GGTACCCAGG CAAGCAGGGC CCCCATGGGC ACCCTGGCCC TCGGGGCGTT 
CCTGGCATCG TGGGAGCCGT GGGTCAGATC GGCAACACGG GGCCCAAGGG AAAACGTGGA 
GAGAAGGGTG ATCCAGGAGA AGTGGGACGG GGGCACCCCG GGATGCCTGG GCCCCCAGGG 
ATCCCAGGAC TCCCTGGCCG GCCTGGCCAG GCAATCAACG GCAAGGATGG AGATCGAGGG 
TCCCCAGGGG CTCCAGGAGA GGCAGGTCGA CCTGGCCTGC CAGGCCCCGT GGGGCTGCCG 
GGCTTCTGTG AACCTGCCGC CTGCCTTGGA GCTTCGGCCT ATGCCTCTGC CCGCCTTACA 
GAGCCTGGAT CCATCAAGGG GCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539019.1
CDS1503..3029
Misc Feature(1)1734..1907(+)
Misc Feature(2)1941..2117(+)
Misc Feature(3)2040..2201(+)
Misc Feature(4)2235..2408(+)
Translation

Target ORF information:

RefSeq Version XM_011540717
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011540717

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAAGGTC CCCCAGGGCT GCAGGGAGTG AAGGGGCATG CGGGCAAACG CGGGATTCTG 
GGTGATCCTG GCCACCAGGG GAAGCCGGGT CCCAAGGGAG ATGTGGGTGC CTCTGGAGAG 
CAAGGCATCC CTGGACCACC GGGTCCCCAG GGCATCAGGG GCTACCCAGG CATGGCAGGG 
CCCAAGGGAG AGACGGGCCC TCATGGATAT AAAGGCATGG TGGGCGCTAT CGGTGCCACT 
GGGCCACCGG GTGAGGAAGG TCCTAGGGGA CCGCCAGGCC GAGCTGGGGA GAAGGGTGAC 
GAGGGCAGCC CAGGTATTCG TGGACCCCAG GGGATCACAG GCCCGAAAGG AGCAACGGGC 
CCCCCAGGCA TCAACGGCAA GGATGGGACC CCAGGCACGC CTGGCATGAA GGGCAGTGCA 
GGACAGGCGG GACAGCCCGG AAGTCCAGGC CACCAGGGCC TAGCGGGTGT GCCAGGCCAG 
CCTGGGACAA AAGGAGGCCC TGGAGACCAG GGTGAGCCGG GCCCGCAGGG CCTTCCTGGA 
TTCTCTGGTC CCCCTGGGAA AGAGGGAGAG CCAGGGCCTC GAGGAGAAAT TGGTCCCCAG 
GGCATCATGG GACAGAAGGG TGACCAAGGC GAGAGGGGTC CAGTGGGGCA ACCAGGCCCT 
CAGGGAAGGC AGGGCCCTAA GGGGGAGCAG GGCCCCCCCG GAATTCCAGG GCCCCAAGGC 
TTGCCAGGCG TCAAAGGAGA CAAGGGCTCC CCAGGGAAGA CCGGGCCCCG CGGCAAAGTG 
GGTGACCCAG GGGTGGCCGG CCTCCCCGGA GAGAAAGGCG AGAAGGGCGA GTCCGGCGAG 
CCGGGGCCCA AGGGACAGCA AGGAGTACGT GGAGAACCCG GCTACCCTGG CCCCAGCGGG 
GATGCGGGCG CCCCAGGGGT TCAGGGCTAC CCTGGTCCCC CCGGCCCTCG AGGACTGGCC 
GGGAACCGAG GCGTGCCAGG ACAGCCCGGG AGACAGGGCG TGGAGGGCCG GGATGCCACT 
GACCAGCACA TCGTGGATGT GGCGCTGAAG ATGCTGCAAG AGCAACTGGC AGAGGTCGCC 
GTGAGTGCCA AGCGGGAAGC CCTGGGTGCG GTGGGCATGA TGGGTCCTCC AGGACCTCCT 
GGGCCCCCTG GGTACCCAGG CAAGCAGGGC CCCCATGGGC ACCCTGGCCC TCGGGGCGTT 
CCTGGCATCG TGGGAGCCGT GGGTCAGATC GGCAACACGG GGCCCAAGGG AAAACGTGGA 
GAGAAGGGTG ATCCAGGAGA AGTGGGACGG GGGCACCCCG GGATGCCTGG GCCCCCAGGG 
ATCCCAGGAC TCCCTGGCCG GCCTGGCCAG GCAATCAACG GCAAGGATGG AGATCGAGGG 
TCCCCAGGGG CTCCAGGAGA GGCAGGTCGA CCTGGCCTGC CAGGCCCCGT GGGGCTGCCG 
GGCTTCTGTG AACCTGCCGC CTGCCTTGGA GCTTCGGCCT ATGCCTCTGC CCGCCTTACA 
GAGCCTGGAT CCATCAAGGG GCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu57000
Clone ID Related Accession (Same CDS sequence) XM_011540718
Accession Version XM_011540718.1 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1215bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 12-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-2(IX) chain isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_032977.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 107 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGG TGAGAGACGA GTGTCCAACC 
AACTGTCCAC CCGGAATGAA AGGTCCCCCA GGGCTGCAGG GAGTGAAGGG GCATGCGGGC 
AAACGCGGGA TTCTGGGTGA TCCTGGCCAC CAGGGGAAGC CGGGTCCCAA GGGAGATGTG 
GGTGCCTCTG GAGAGCAAGG CATCCCTGGA CCACCGGGTC CCCAGGGCAT CAGGGGCTAC 
CCAGGCATGG CAGGGCCCAA GGGAGAGACG GGCCCTCATG GATATAAAGG CATGGTGGGC 
GCTATCGGTG CCACTGGGCC ACCGGGTGAG GAAGGTCCTA GGGGACCGCC AGGCCGAGCT 
GGGGAGAAGG GTGACGAGGG CAGCCCAGGT ATTCGTGGAC CCCAGGGGAT CACAGGCCCG 
AAAGGAGCAA CGGGCCCCCC AGGCATCAAC GGCAAGGATG GGACCCCAGG CACGCCTGGC 
ATGAAGGGCA GTGCAGGACA GGCGGGACAG CCCGGAAGTC CAGGCCACCA GGGCCTAGCG 
ACCCCAGGTC CTGGAAGACA GGCCATTCTC TGTGCCCCAC TCCTCTGCCA CCACTGTGGG 
AATGTACAGT TAAGTTCATC ATGCCGTGGC TGGACCTTCT GTTGTTCAGC TCCCACAGGT 
GGGGGAAATC TGGATTGGGG ATGGGAAGCA AAGCAGCAGG TGCATGGGGC TCCCTCATGC 
CAGGGCAGAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539020.1
CDS216..1430
Misc Feature(1)1002..1175(+)
Translation

Target ORF information:

RefSeq Version XM_011540718
Organism Homo sapiens (human)
Definition PREDICTED: Homo sapiens collagen, type IX, alpha 2 (COL9A2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 XM_011540718

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGG TGAGAGACGA GTGTCCAACC 
AACTGTCCAC CCGGAATGAA AGGTCCCCCA GGGCTGCAGG GAGTGAAGGG GCATGCGGGC 
AAACGCGGGA TTCTGGGTGA TCCTGGCCAC CAGGGGAAGC CGGGTCCCAA GGGAGATGTG 
GGTGCCTCTG GAGAGCAAGG CATCCCTGGA CCACCGGGTC CCCAGGGCAT CAGGGGCTAC 
CCAGGCATGG CAGGGCCCAA GGGAGAGACG GGCCCTCATG GATATAAAGG CATGGTGGGC 
GCTATCGGTG CCACTGGGCC ACCGGGTGAG GAAGGTCCTA GGGGACCGCC AGGCCGAGCT 
GGGGAGAAGG GTGACGAGGG CAGCCCAGGT ATTCGTGGAC CCCAGGGGAT CACAGGCCCG 
AAAGGAGCAA CGGGCCCCCC AGGCATCAAC GGCAAGGATG GGACCCCAGG CACGCCTGGC 
ATGAAGGGCA GTGCAGGACA GGCGGGACAG CCCGGAAGTC CAGGCCACCA GGGCCTAGCG 
ACCCCAGGTC CTGGAAGACA GGCCATTCTC TGTGCCCCAC TCCTCTGCCA CCACTGTGGG 
AATGTACAGT TAAGTTCATC ATGCCGTGGC TGGACCTTCT GTTGTTCAGC TCCCACAGGT 
GGGGGAAATC TGGATTGGGG ATGGGAAGCA AAGCAGCAGG TGCATGGGGC TCCCTCATGC 
CAGGGCAGAA ACTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24811
Clone ID Related Accession (Same CDS sequence) NM_001852 , XM_006710365
Accession Version NM_001852.3 Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Structure linear
Update Date 15-MAR-2015
Organism Homo sapiens (human)
Product collagen alpha-2(IX) chain precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF019406.1 and AL050341.18. This sequence is a reference standard in the RefSeqGene project. On May 27, 2003 this sequence version replaced gi:17978505. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95610.1, BC136326.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length. 

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC 
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT 
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA 
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA 
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC 
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT 
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG 
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT 
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC 
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT 
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC 
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC 
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA 
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA 
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC 
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC 
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG 
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC 
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC 
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT 
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC 
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT 
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA 
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA 
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG 
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT 
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001843.1
CDS71..2140
Misc Feature(1)149..559(+)
Misc Feature(2)560..610(+)
Misc Feature(3)611..1627(+)
Misc Feature(4)845..1018(+)
Misc Feature(5)1052..1228(+)
Misc Feature(6)1151..1312(+)
Misc Feature(7)1346..1519(+)
Misc Feature(8)1628..1717(+)
Misc Feature(9)1718..1966(+)
Misc Feature(10)1967..1972(+)
Misc Feature(11)1973..2062(+)
Misc Feature(12)2063..2137(+)
Exon (1)1..145
Gene:COL9A2
Gene Synonym:
Exon (2)146..220
Gene:COL9A2
Gene Synonym:
Exon (3)221..256
Gene:COL9A2
Gene Synonym:
Exon (4)257..319
Gene:COL9A2
Gene Synonym:
Exon (5)320..373
Gene:COL9A2
Gene Synonym:
Exon (6)374..409
Gene:COL9A2
Gene Synonym:
Exon (7)410..433
Gene:COL9A2
Gene Synonym:
Exon (8)434..487
Gene:COL9A2
Gene Synonym:
Exon (9)488..541
Gene:COL9A2
Gene Synonym:
Exon (10)542..589
Gene:COL9A2
Gene Synonym:
Exon (11)590..646
Gene:COL9A2
Gene Synonym:
Exon (12)647..700
Gene:COL9A2
Gene Synonym:
Exon (13)701..754
Gene:COL9A2
Gene Synonym:
Exon (14)755..808
Gene:COL9A2
Gene Synonym:
Exon (15)809..862
Gene:COL9A2
Gene Synonym:
Exon (16)863..916
Gene:COL9A2
Gene Synonym:
Exon (17)917..970
Gene:COL9A2
Gene Synonym:
Exon (18)971..1024
Gene:COL9A2
Gene Synonym:
Exon (19)1025..1078
Gene:COL9A2
Gene Synonym:
Exon (20)1079..1123
Gene:COL9A2
Gene Synonym:
Exon (21)1124..1177
Gene:COL9A2
Gene Synonym:
Exon (22)1178..1231
Gene:COL9A2
Gene Synonym:
Exon (23)1232..1285
Gene:COL9A2
Gene Synonym:
Exon (24)1286..1357
Gene:COL9A2
Gene Synonym:
Exon (25)1358..1393
Gene:COL9A2
Gene Synonym:
Exon (26)1394..1438
Gene:COL9A2
Gene Synonym:
Exon (27)1439..1471
Gene:COL9A2
Gene Synonym:
Exon (28)1472..1618
Gene:COL9A2
Gene Synonym:
Exon (29)1619..1673
Gene:COL9A2
Gene Synonym:
Exon (30)1674..1862
Gene:COL9A2
Gene Synonym:
Exon (31)1863..1940
Gene:COL9A2
Gene Synonym:
Exon (32)1941..2831
Gene:COL9A2
Gene Synonym:
Translation

Target ORF information:

RefSeq Version NM_001852
Organism Homo sapiens (human)
Definition Homo sapiens collagen, type IX, alpha 2 (COL9A2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
 NM_001852

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG 
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC 
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC 
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC 
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT 
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG 
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG 
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC 
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT 
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA 
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA 
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC 
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT 
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG 
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT 
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC 
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT 
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC 
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC 
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA 
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA 
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC 
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC 
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG 
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC 
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC 
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT 
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC 
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT 
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA 
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA 
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG 
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT 
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

PubMed

 
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