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COL9A2 cDNA ORF clone, Homo sapiens(Human)

Gene Symbol COL9A2
Entrez Gene ID 1298
Full Name collagen type IX alpha 2 chain
Synonyms DJ39G22.4,EDM2,MED,STL5
General protein information
Preferred Names

collagen type IX alpha 2 chain

Names

collagen alpha-2(IX) chain
alpha 2 type IX collagen
collagen IX, alpha-2 polypeptide
collagen, type IX, alpha 2

Gene Type protein-coding
Organism Homo sapiens(Human)
Genome

1

1p34.2

Summary This gene encodes one of the three alpha chains of type IX collagen, the major collagen component of hyaline cartilage. Type IX collagen, a heterotrimeric molecule, is usually found in tissues containing type II collagen, a fibrillar collagen. This chain is unusual in that, unlike the other two type IX alpha chains, it contains a covalently attached glycosaminoglycan side chain. Mutations in this gene are associated with multiple epiphyseal dysplasia. [provided by RefSeq, Jul 2008].
Disorder MIM:

120260

Disorder Html: Epiphyseal dysplasia, multiple, 2, 600204 (3); {Intervertebral

mRNA and Protein(s)

mRNA Protein Name
NM_001852.3 NP_001843.1 collagen alpha-2(IX) chain precursor
XM_017000332.1 XP_016855821.1 collagen alpha-2(IX) chain isoform X1
XM_006710365.3 XP_006710428.1 collagen alpha-2(IX) chain isoform X2
XM_011540717.2 XP_011539019.1 collagen alpha-2(IX) chain isoform X5
XM_011540715.2 XP_011539017.1 collagen alpha-2(IX) chain isoform X3
XM_017000333.1 XP_016855822.1 collagen alpha-2(IX) chain isoform X4
XM_011540716.2 XP_011539018.1 collagen alpha-2(IX) chain isoform X3



Pan troglodytes (chimpanzee) COL9A2 XP_003949434.1
Canis lupus familiaris (dog) COL9A2 NP_001184099.1
Xenopus tropicalis (tropical clawed frog) col9a2 NP_001090661.1
Macaca mulatta (rhesus monkey) COL9A2 XP_001082953.2
Bos taurus (cattle) COL9A2 XP_002686588.2
Mus musculus (house mouse) Col9a2 NP_031767.2
Rattus norvegicus (Norway rat) Col9a2 NP_001102145.1
Danio rerio (zebrafish) col9a2 NP_997744.1
Gallus gallus (chicken) COL9A2 NP_001258917.1
Homo sapiens (human) COL9A2 NP_001843.1


Related articles in PubMed

Association between COL9A2 Gln326Arg mutations and the development of intervertebral disc disease in a Chinese population.
Meng T, Ren Q, Wang JM, Shi H, Zhang ST, Liu MT
Genetics and molecular research : GMR15(4)(2016 Dec)

Meta-analysis of the association between COL9A2 genetic polymorphisms and lumbar disc disease susceptibility.
Zhang Z, Zhang J, Ding L, Teng X
Spine39(20)1699-706(2014 Sep)

[Analysis of COL9A2 gene mutations in a Chinese Han population with pathological myopia].
Chen R, Gong B, Li Q, Zeng G, Hao F, Li N, Shi Y, Zhang D
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics31(2)129-33(2014 Apr)

The role of polymorphisms of genes encoding collagen IX and XI in lumbar disc disease.
Janeczko Ł, Janeczko M, Chrzanowski R, Zieliński G
Neurologia i neurochirurgia polska48(1)60-2(2014 Jan-Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Study provides evidence that the COL9A2 Gln326Arg polymorphism contributes to the development of intervertebral disc disease in the Chinese population.
Title: Association between COL9A2 Gln326Arg mutations and the development of intervertebral disc disease in a Chinese population.

Meta-analysis. Our results suggest that the COL9A2 rs12077871, rs12722877, and rs7533552 polymorphisms may not be associated with lumbar disc disease.
Title: Meta-analysis of the association between COL9A2 genetic polymorphisms and lumbar disc disease susceptibility.

Two novel mutations, c.143G>C in exon 2 and c.884G>A in exon 17 of the COL9A2 gene, may contribute to the development of pathological myopia.
Title: [Analysis of COL9A2 gene mutations in a Chinese Han population with pathological myopia].

The allelic variants in the collagen IX genes - COL9A2 and COL9A3 have been identified as genetic risk factors for intervertebral disc disease--{review}
Title: The role of polymorphisms of genes encoding collagen IX and XI in lumbar disc disease.

The NC2 domain of type IX collagen determines the chain composition but also the chain register of the triple helix.
Title: The NC2 domain of type IX collagen determines the chain register of the triple helix.

The following COL9A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL9A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID RefSeq Accession Definition **Vector *Turnaround time Price (USD) Select
OHu56998 XM_011540716.2
Latest version!
Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X4, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu56998 XM_011540715.2
Latest version!
Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X3, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu56999 XM_011540717.2
Latest version!
Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X6, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 7-9 $307.30
$439.00
OHu24811 NM_001852.3
Latest version!
Homo sapiens collagen type IX alpha 2 chain (COL9A2), mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu24811 XM_006710365.3
Latest version!
Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X2, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $265.30
$379.00
OHu56997 XM_017000332.1
Latest version!
Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X1, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 12-14 $391.30
$559.00
OHu83146 XM_017000333.1
Latest version!
Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X5, mRNA. pcDNA3.1+/C-(K)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.


CloneID OHu56998
Clone ID Related Accession (Same CDS sequence) XM_011540715.2 , XM_011540716.2
Accession Version XM_011540715.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-2(IX) chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540715.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539017.1
CDS929..2728
Misc Feature(1)1433..1606
Misc Feature(2)1640..1816
Misc Feature(3)1739..1900
Misc Feature(4)1934..2107
Translation

Target ORF information:

RefSeq Version XM_011540715.2
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X3, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011540715.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56998
Clone ID Related Accession (Same CDS sequence) XM_011540715.2 , XM_011540716.2
Accession Version XM_011540716.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1800bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-2(IX) chain isoform X3
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540716.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539018.1
CDS409..2208
Misc Feature(1)913..1086
Misc Feature(2)1120..1296
Misc Feature(3)1219..1380
Misc Feature(4)1414..1587
Translation

Target ORF information:

RefSeq Version XM_011540716.2
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X4, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011540716.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGGTG
AGAGACGAGT GTCCAACCAA CTGTCCACCC GGAATGAAAG GTCCCCCAGG GCTGCAGGGA
GTGAAGGGGC ATGCGGGCAA ACGCGGGATT CTGGGTGATC CTGGCCACCA GGGGAAGCCG
GGTCCCAAGG GAGATGTGGG TGCCTCTGGA GAGCAAGGCA TCCCTGGACC ACCGGGTCCC
CAGGGCATCA GGGGCTACCC AGGCATGGCA GGGCCCAAGG GAGAGACGGG CCCTCATGGA
TATAAAGGCA TGGTGGGCGC TATCGGTGCC ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG
GGACCGCCAG GCCGAGCTGG GGAGAAGGGT GACGAGGGCA GCCCAGGTAT TCGTGGACCC
CAGGGGATCA CAGGCCCGAA AGGAGCAACG GGCCCCCCAG GCATCAACGG CAAGGATGGG
ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT GCAGGACAGG CGGGACAGCC CGGAAGTCCA
GGCCACCAGG GCCTAGCGGG TGTGCCAGGC CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC
CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT GGATTCTCTG GTCCCCCTGG GAAAGAGGGA
GAGCCAGGGC CTCGAGGAGA AATTGGTCCC CAGGGCATCA TGGGACAGAA GGGTGACCAA
GGCGAGAGGG GTCCAGTGGG GCAACCAGGC CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG
CAGGGCCCCC CCGGAATTCC AGGGCCCCAA GGCTTGCCAG GCGTCAAAGG AGACAAGGGC
TCCCCAGGGA AGACCGGGCC CCGCGGCAAA GTGGGTGACC CAGGGGTGGC CGGCCTCCCC
GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC GAGCCGGGGC CCAAGGGACA GCAAGGAGTA
CGTGGAGAAC CCGGCTACCC TGGCCCCAGC GGGGATGCGG GCGCCCCAGG GGTTCAGGGC
TACCCTGGTC CCCCCGGCCC TCGAGGACTG GCCGGGAACC GAGGCGTGCC AGGACAGCCC
GGGAGACAGG GCGTGGAGGG CCGGGATGCC ACTGACCAGC ACATCGTGGA TGTGGCGCTG
AAGATGCTGC AAGAGCAACT GGCAGAGGTC GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT
GCGGTGGGCA TGATGGGTCC TCCAGGACCT CCTGGGCCCC CTGGGTACCC AGGCAAGCAG
GGCCCCCATG GGCACCCTGG CCCTCGGGGC GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG
ATCGGCAACA CGGGGCCCAA GGGAAAACGT GGAGAGAAGG GTGATCCAGG AGAAGTGGGA
CGGGGGCACC CCGGGATGCC TGGGCCCCCA GGGATCCCAG GACTCCCTGG CCGGCCTGGC
CAGGCAATCA ACGGCAAGGA TGGAGATCGA GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT
CGACCTGGCC TGCCAGGCCC CGTGGGGCTG CCGGGCTTCT GTGAACCTGC CGCCTGCCTT
GGAGCTTCGG CCTATGCCTC TGCCCGCCTT ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56999
Clone ID Related Accession (Same CDS sequence) XM_011540717.2
Accession Version XM_011540717.2 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1527bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-2(IX) chain isoform X5
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540717.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAAGGTC CCCCAGGGCT GCAGGGAGTG AAGGGGCATG CGGGCAAACG CGGGATTCTG 
GGTGATCCTG GCCACCAGGG GAAGCCGGGT CCCAAGGGAG ATGTGGGTGC CTCTGGAGAG
CAAGGCATCC CTGGACCACC GGGTCCCCAG GGCATCAGGG GCTACCCAGG CATGGCAGGG
CCCAAGGGAG AGACGGGCCC TCATGGATAT AAAGGCATGG TGGGCGCTAT CGGTGCCACT
GGGCCACCGG GTGAGGAAGG TCCTAGGGGA CCGCCAGGCC GAGCTGGGGA GAAGGGTGAC
GAGGGCAGCC CAGGTATTCG TGGACCCCAG GGGATCACAG GCCCGAAAGG AGCAACGGGC
CCCCCAGGCA TCAACGGCAA GGATGGGACC CCAGGCACGC CTGGCATGAA GGGCAGTGCA
GGACAGGCGG GACAGCCCGG AAGTCCAGGC CACCAGGGCC TAGCGGGTGT GCCAGGCCAG
CCTGGGACAA AAGGAGGCCC TGGAGACCAG GGTGAGCCGG GCCCGCAGGG CCTTCCTGGA
TTCTCTGGTC CCCCTGGGAA AGAGGGAGAG CCAGGGCCTC GAGGAGAAAT TGGTCCCCAG
GGCATCATGG GACAGAAGGG TGACCAAGGC GAGAGGGGTC CAGTGGGGCA ACCAGGCCCT
CAGGGAAGGC AGGGCCCTAA GGGGGAGCAG GGCCCCCCCG GAATTCCAGG GCCCCAAGGC
TTGCCAGGCG TCAAAGGAGA CAAGGGCTCC CCAGGGAAGA CCGGGCCCCG CGGCAAAGTG
GGTGACCCAG GGGTGGCCGG CCTCCCCGGA GAGAAAGGCG AGAAGGGCGA GTCCGGCGAG
CCGGGGCCCA AGGGACAGCA AGGAGTACGT GGAGAACCCG GCTACCCTGG CCCCAGCGGG
GATGCGGGCG CCCCAGGGGT TCAGGGCTAC CCTGGTCCCC CCGGCCCTCG AGGACTGGCC
GGGAACCGAG GCGTGCCAGG ACAGCCCGGG AGACAGGGCG TGGAGGGCCG GGATGCCACT
GACCAGCACA TCGTGGATGT GGCGCTGAAG ATGCTGCAAG AGCAACTGGC AGAGGTCGCC
GTGAGTGCCA AGCGGGAAGC CCTGGGTGCG GTGGGCATGA TGGGTCCTCC AGGACCTCCT
GGGCCCCCTG GGTACCCAGG CAAGCAGGGC CCCCATGGGC ACCCTGGCCC TCGGGGCGTT
CCTGGCATCG TGGGAGCCGT GGGTCAGATC GGCAACACGG GGCCCAAGGG AAAACGTGGA
GAGAAGGGTG ATCCAGGAGA AGTGGGACGG GGGCACCCCG GGATGCCTGG GCCCCCAGGG
ATCCCAGGAC TCCCTGGCCG GCCTGGCCAG GCAATCAACG GCAAGGATGG AGATCGAGGG
TCCCCAGGGG CTCCAGGAGA GGCAGGTCGA CCTGGCCTGC CAGGCCCCGT GGGGCTGCCG
GGCTTCTGTG AACCTGCCGC CTGCCTTGGA GCTTCGGCCT ATGCCTCTGC CCGCCTTACA
GAGCCTGGAT CCATCAAGGG GCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_011539019.1
CDS1530..3056
Misc Feature(1)1761..1934
Misc Feature(2)1968..2144
Misc Feature(3)2067..2228
Misc Feature(4)2262..2435
Translation

Target ORF information:

RefSeq Version XM_011540717.2
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X6, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_011540717.2

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
ATGAAAGGTC CCCCAGGGCT GCAGGGAGTG AAGGGGCATG CGGGCAAACG CGGGATTCTG 
GGTGATCCTG GCCACCAGGG GAAGCCGGGT CCCAAGGGAG ATGTGGGTGC CTCTGGAGAG
CAAGGCATCC CTGGACCACC GGGTCCCCAG GGCATCAGGG GCTACCCAGG CATGGCAGGG
CCCAAGGGAG AGACGGGCCC TCATGGATAT AAAGGCATGG TGGGCGCTAT CGGTGCCACT
GGGCCACCGG GTGAGGAAGG TCCTAGGGGA CCGCCAGGCC GAGCTGGGGA GAAGGGTGAC
GAGGGCAGCC CAGGTATTCG TGGACCCCAG GGGATCACAG GCCCGAAAGG AGCAACGGGC
CCCCCAGGCA TCAACGGCAA GGATGGGACC CCAGGCACGC CTGGCATGAA GGGCAGTGCA
GGACAGGCGG GACAGCCCGG AAGTCCAGGC CACCAGGGCC TAGCGGGTGT GCCAGGCCAG
CCTGGGACAA AAGGAGGCCC TGGAGACCAG GGTGAGCCGG GCCCGCAGGG CCTTCCTGGA
TTCTCTGGTC CCCCTGGGAA AGAGGGAGAG CCAGGGCCTC GAGGAGAAAT TGGTCCCCAG
GGCATCATGG GACAGAAGGG TGACCAAGGC GAGAGGGGTC CAGTGGGGCA ACCAGGCCCT
CAGGGAAGGC AGGGCCCTAA GGGGGAGCAG GGCCCCCCCG GAATTCCAGG GCCCCAAGGC
TTGCCAGGCG TCAAAGGAGA CAAGGGCTCC CCAGGGAAGA CCGGGCCCCG CGGCAAAGTG
GGTGACCCAG GGGTGGCCGG CCTCCCCGGA GAGAAAGGCG AGAAGGGCGA GTCCGGCGAG
CCGGGGCCCA AGGGACAGCA AGGAGTACGT GGAGAACCCG GCTACCCTGG CCCCAGCGGG
GATGCGGGCG CCCCAGGGGT TCAGGGCTAC CCTGGTCCCC CCGGCCCTCG AGGACTGGCC
GGGAACCGAG GCGTGCCAGG ACAGCCCGGG AGACAGGGCG TGGAGGGCCG GGATGCCACT
GACCAGCACA TCGTGGATGT GGCGCTGAAG ATGCTGCAAG AGCAACTGGC AGAGGTCGCC
GTGAGTGCCA AGCGGGAAGC CCTGGGTGCG GTGGGCATGA TGGGTCCTCC AGGACCTCCT
GGGCCCCCTG GGTACCCAGG CAAGCAGGGC CCCCATGGGC ACCCTGGCCC TCGGGGCGTT
CCTGGCATCG TGGGAGCCGT GGGTCAGATC GGCAACACGG GGCCCAAGGG AAAACGTGGA
GAGAAGGGTG ATCCAGGAGA AGTGGGACGG GGGCACCCCG GGATGCCTGG GCCCCCAGGG
ATCCCAGGAC TCCCTGGCCG GCCTGGCCAG GCAATCAACG GCAAGGATGG AGATCGAGGG
TCCCCAGGGG CTCCAGGAGA GGCAGGTCGA CCTGGCCTGC CAGGCCCCGT GGGGCTGCCG
GGCTTCTGTG AACCTGCCGC CTGCCTTGGA GCTTCGGCCT ATGCCTCTGC CCGCCTTACA
GAGCCTGGAT CCATCAAGGG GCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24811
Clone ID Related Accession (Same CDS sequence) NM_001852.3 , XM_006710365.3
Accession Version NM_001852.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2017-04-25
Organism Homo sapiens(Human)
Product collagen alpha-2(IX) chain precursor
Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF019406.1 and AL050341.18. This sequence is a reference standard in the RefSeqGene project. On May 27, 2003 this sequence version replaced NM_001852.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M95610.1, BC136326.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq NP_001843.1
CDS71..2140
Misc Feature(1)149..559
Misc Feature(2)560..610
Misc Feature(3)611..1627
Misc Feature(4)845..1018
Misc Feature(5)1052..1228
Misc Feature(6)1151..1312
Misc Feature(7)1346..1519
Misc Feature(8)1628..1717
Misc Feature(9)1718..1966
Misc Feature(10)1967..1972
Misc Feature(11)1973..2062
Misc Feature(12)2063..2137
Exon (1)1..145
Exon (2)146..220
Exon (3)221..256
Exon (4)257..319
Exon (5)320..373
Exon (6)374..409
Exon (7)410..433
Exon (8)434..487
Exon (9)488..541
Exon (10)542..589
Exon (11)590..646
Exon (12)647..700
Exon (13)701..754
Exon (14)755..808
Exon (15)809..862
Exon (16)863..916
Exon (17)917..970
Exon (18)971..1024
Exon (19)1025..1078
Exon (20)1079..1123
Exon (21)1124..1177
Exon (22)1178..1231
Exon (23)1232..1285
Exon (24)1286..1357
Exon (25)1358..1393
Exon (26)1394..1438
Exon (27)1439..1471
Exon (28)1472..1618
Exon (29)1619..1673
Exon (30)1674..1862
Exon (31)1863..1940
Exon (32)1941..2831
Translation

Target ORF information:

RefSeq Version NM_001852.3
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type IX alpha 2 chain (COL9A2), mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
NM_001852.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu24811
Clone ID Related Accession (Same CDS sequence) NM_001852.3 , XM_006710365.3
Accession Version XM_006710365.3 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2070bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-2(IX) chain isoform X2
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006710365.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_006710428.1
CDS179..2248
Misc Feature(1)953..1126
Misc Feature(2)1160..1336
Misc Feature(3)1259..1420
Misc Feature(4)1454..1627
Translation

Target ORF information:

RefSeq Version XM_006710365.3
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X2, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_006710365.3

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGT GTCCAACCAA CTGTCCACCC
GGAATGAAAG GTCCCCCAGG GCTGCAGGGA GTGAAGGGGC ATGCGGGCAA ACGCGGGATT
CTGGGTGATC CTGGCCACCA GGGGAAGCCG GGTCCCAAGG GAGATGTGGG TGCCTCTGGA
GAGCAAGGCA TCCCTGGACC ACCGGGTCCC CAGGGCATCA GGGGCTACCC AGGCATGGCA
GGGCCCAAGG GAGAGACGGG CCCTCATGGA TATAAAGGCA TGGTGGGCGC TATCGGTGCC
ACTGGGCCAC CGGGTGAGGA AGGTCCTAGG GGACCGCCAG GCCGAGCTGG GGAGAAGGGT
GACGAGGGCA GCCCAGGTAT TCGTGGACCC CAGGGGATCA CAGGCCCGAA AGGAGCAACG
GGCCCCCCAG GCATCAACGG CAAGGATGGG ACCCCAGGCA CGCCTGGCAT GAAGGGCAGT
GCAGGACAGG CGGGACAGCC CGGAAGTCCA GGCCACCAGG GCCTAGCGGG TGTGCCAGGC
CAGCCTGGGA CAAAAGGAGG CCCTGGAGAC CAGGGTGAGC CGGGCCCGCA GGGCCTTCCT
GGATTCTCTG GTCCCCCTGG GAAAGAGGGA GAGCCAGGGC CTCGAGGAGA AATTGGTCCC
CAGGGCATCA TGGGACAGAA GGGTGACCAA GGCGAGAGGG GTCCAGTGGG GCAACCAGGC
CCTCAGGGAA GGCAGGGCCC TAAGGGGGAG CAGGGCCCCC CCGGAATTCC AGGGCCCCAA
GGCTTGCCAG GCGTCAAAGG AGACAAGGGC TCCCCAGGGA AGACCGGGCC CCGCGGCAAA
GTGGGTGACC CAGGGGTGGC CGGCCTCCCC GGAGAGAAAG GCGAGAAGGG CGAGTCCGGC
GAGCCGGGGC CCAAGGGACA GCAAGGAGTA CGTGGAGAAC CCGGCTACCC TGGCCCCAGC
GGGGATGCGG GCGCCCCAGG GGTTCAGGGC TACCCTGGTC CCCCCGGCCC TCGAGGACTG
GCCGGGAACC GAGGCGTGCC AGGACAGCCC GGGAGACAGG GCGTGGAGGG CCGGGATGCC
ACTGACCAGC ACATCGTGGA TGTGGCGCTG AAGATGCTGC AAGAGCAACT GGCAGAGGTC
GCCGTGAGTG CCAAGCGGGA AGCCCTGGGT GCGGTGGGCA TGATGGGTCC TCCAGGACCT
CCTGGGCCCC CTGGGTACCC AGGCAAGCAG GGCCCCCATG GGCACCCTGG CCCTCGGGGC
GTTCCTGGCA TCGTGGGAGC CGTGGGTCAG ATCGGCAACA CGGGGCCCAA GGGAAAACGT
GGAGAGAAGG GTGATCCAGG AGAAGTGGGA CGGGGGCACC CCGGGATGCC TGGGCCCCCA
GGGATCCCAG GACTCCCTGG CCGGCCTGGC CAGGCAATCA ACGGCAAGGA TGGAGATCGA
GGGTCCCCAG GGGCTCCAGG AGAGGCAGGT CGACCTGGCC TGCCAGGCCC CGTGGGGCTG
CCGGGCTTCT GTGAACCTGC CGCCTGCCTT GGAGCTTCGG CCTATGCCTC TGCCCGCCTT
ACAGAGCCTG GATCCATCAA GGGGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu56997
Clone ID Related Accession (Same CDS sequence) XM_017000332.1
Accession Version XM_017000332.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 2082bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-2(IX) chain isoform X1
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGG TGAGAGACGA GTGTCCAACC
AACTGTCCAC CCGGAATGAA AGGTCCCCCA GGGCTGCAGG GAGTGAAGGG GCATGCGGGC
AAACGCGGGA TTCTGGGTGA TCCTGGCCAC CAGGGGAAGC CGGGTCCCAA GGGAGATGTG
GGTGCCTCTG GAGAGCAAGG CATCCCTGGA CCACCGGGTC CCCAGGGCAT CAGGGGCTAC
CCAGGCATGG CAGGGCCCAA GGGAGAGACG GGCCCTCATG GATATAAAGG CATGGTGGGC
GCTATCGGTG CCACTGGGCC ACCGGGTGAG GAAGGTCCTA GGGGACCGCC AGGCCGAGCT
GGGGAGAAGG GTGACGAGGG CAGCCCAGGT ATTCGTGGAC CCCAGGGGAT CACAGGCCCG
AAAGGAGCAA CGGGCCCCCC AGGCATCAAC GGCAAGGATG GGACCCCAGG CACGCCTGGC
ATGAAGGGCA GTGCAGGACA GGCGGGACAG CCCGGAAGTC CAGGCCACCA GGGCCTAGCG
GGTGTGCCAG GCCAGCCTGG GACAAAAGGA GGCCCTGGAG ACCAGGGTGA GCCGGGCCCG
CAGGGCCTTC CTGGATTCTC TGGTCCCCCT GGGAAAGAGG GAGAGCCAGG GCCTCGAGGA
GAAATTGGTC CCCAGGGCAT CATGGGACAG AAGGGTGACC AAGGCGAGAG GGGTCCAGTG
GGGCAACCAG GCCCTCAGGG AAGGCAGGGC CCTAAGGGGG AGCAGGGCCC CCCCGGAATT
CCAGGGCCCC AAGGCTTGCC AGGCGTCAAA GGAGACAAGG GCTCCCCAGG GAAGACCGGG
CCCCGCGGCA AAGTGGGTGA CCCAGGGGTG GCCGGCCTCC CCGGAGAGAA AGGCGAGAAG
GGCGAGTCCG GCGAGCCGGG GCCCAAGGGA CAGCAAGGAG TACGTGGAGA ACCCGGCTAC
CCTGGCCCCA GCGGGGATGC GGGCGCCCCA GGGGTTCAGG GCTACCCTGG TCCCCCCGGC
CCTCGAGGAC TGGCCGGGAA CCGAGGCGTG CCAGGACAGC CCGGGAGACA GGGCGTGGAG
GGCCGGGATG CCACTGACCA GCACATCGTG GATGTGGCGC TGAAGATGCT GCAAGAGCAA
CTGGCAGAGG TCGCCGTGAG TGCCAAGCGG GAAGCCCTGG GTGCGGTGGG CATGATGGGT
CCTCCAGGAC CTCCTGGGCC CCCTGGGTAC CCAGGCAAGC AGGGCCCCCA TGGGCACCCT
GGCCCTCGGG GCGTTCCTGG CATCGTGGGA GCCGTGGGTC AGATCGGCAA CACGGGGCCC
AAGGGAAAAC GTGGAGAGAA GGGTGATCCA GGAGAAGTGG GACGGGGGCA CCCCGGGATG
CCTGGGCCCC CAGGGATCCC AGGACTCCCT GGCCGGCCTG GCCAGGCAAT CAACGGCAAG
GATGGAGATC GAGGGTCCCC AGGGGCTCCA GGAGAGGCAG GTCGACCTGG CCTGCCAGGC
CCCGTGGGGC TGCCGGGCTT CTGTGAACCT GCCGCCTGCC TTGGAGCTTC GGCCTATGCC
TCTGCCCGCC TTACAGAGCC TGGATCCATC AAGGGGCCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016855821.1
CDS113..2194
Misc Feature(1)899..1072
Misc Feature(2)1106..1282
Misc Feature(3)1205..1366
Misc Feature(4)1400..1573
Translation

Target ORF information:

RefSeq Version XM_017000332.1
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X1, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017000332.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
1801
1861
1921
1981
2041
ATGGCCGCCG CTACGGCCTC CCCCCGCAGC CTCCTTGTTC TCCTCCAGGT GGTAGTGCTC 
GCTCTGGCGC AGATTAGAGG TCCACCGGGA GAGCGGGGCC CCCCGGGTCC CCCGGGACCG
CCGGGAGTGC CTGGATCCGA CGGCATCGAC GGTGACAATG GGCCCCCTGG AAAAGCTGGC
CCTCCGGGAC CCAAGGGCGA GCCTGGCAAA GCTGGGCCAG ATGGGCCAGA CGGGAAGCCC
GGGATTGATG GTTTAACTGG AGCCAAGGGG GAGCCTGGCC CCATGGGGAT CCCTGGAGTC
AAGGGCCAGC CCGGGCTTCC TGGTCCTCCT GGCCTTCCGG GCCCTGGTTT TGCTGGACCT
CCTGGGCCTC CTGGACCTGT TGGCCTCCCT GGTGAGATTG GAATCCGAGG CCCCAAGGGG
GACCCTGGAC CAGATGGACC ATCGGGGCCC CCAGGACCCC CTGGGAAACC TGGTCGCCCG
GGAACCATCC AGGGTCTGGA AGGCAGTGCG GATTTCCTGG TGAGAGACGA GTGTCCAACC
AACTGTCCAC CCGGAATGAA AGGTCCCCCA GGGCTGCAGG GAGTGAAGGG GCATGCGGGC
AAACGCGGGA TTCTGGGTGA TCCTGGCCAC CAGGGGAAGC CGGGTCCCAA GGGAGATGTG
GGTGCCTCTG GAGAGCAAGG CATCCCTGGA CCACCGGGTC CCCAGGGCAT CAGGGGCTAC
CCAGGCATGG CAGGGCCCAA GGGAGAGACG GGCCCTCATG GATATAAAGG CATGGTGGGC
GCTATCGGTG CCACTGGGCC ACCGGGTGAG GAAGGTCCTA GGGGACCGCC AGGCCGAGCT
GGGGAGAAGG GTGACGAGGG CAGCCCAGGT ATTCGTGGAC CCCAGGGGAT CACAGGCCCG
AAAGGAGCAA CGGGCCCCCC AGGCATCAAC GGCAAGGATG GGACCCCAGG CACGCCTGGC
ATGAAGGGCA GTGCAGGACA GGCGGGACAG CCCGGAAGTC CAGGCCACCA GGGCCTAGCG
GGTGTGCCAG GCCAGCCTGG GACAAAAGGA GGCCCTGGAG ACCAGGGTGA GCCGGGCCCG
CAGGGCCTTC CTGGATTCTC TGGTCCCCCT GGGAAAGAGG GAGAGCCAGG GCCTCGAGGA
GAAATTGGTC CCCAGGGCAT CATGGGACAG AAGGGTGACC AAGGCGAGAG GGGTCCAGTG
GGGCAACCAG GCCCTCAGGG AAGGCAGGGC CCTAAGGGGG AGCAGGGCCC CCCCGGAATT
CCAGGGCCCC AAGGCTTGCC AGGCGTCAAA GGAGACAAGG GCTCCCCAGG GAAGACCGGG
CCCCGCGGCA AAGTGGGTGA CCCAGGGGTG GCCGGCCTCC CCGGAGAGAA AGGCGAGAAG
GGCGAGTCCG GCGAGCCGGG GCCCAAGGGA CAGCAAGGAG TACGTGGAGA ACCCGGCTAC
CCTGGCCCCA GCGGGGATGC GGGCGCCCCA GGGGTTCAGG GCTACCCTGG TCCCCCCGGC
CCTCGAGGAC TGGCCGGGAA CCGAGGCGTG CCAGGACAGC CCGGGAGACA GGGCGTGGAG
GGCCGGGATG CCACTGACCA GCACATCGTG GATGTGGCGC TGAAGATGCT GCAAGAGCAA
CTGGCAGAGG TCGCCGTGAG TGCCAAGCGG GAAGCCCTGG GTGCGGTGGG CATGATGGGT
CCTCCAGGAC CTCCTGGGCC CCCTGGGTAC CCAGGCAAGC AGGGCCCCCA TGGGCACCCT
GGCCCTCGGG GCGTTCCTGG CATCGTGGGA GCCGTGGGTC AGATCGGCAA CACGGGGCCC
AAGGGAAAAC GTGGAGAGAA GGGTGATCCA GGAGAAGTGG GACGGGGGCA CCCCGGGATG
CCTGGGCCCC CAGGGATCCC AGGACTCCCT GGCCGGCCTG GCCAGGCAAT CAACGGCAAG
GATGGAGATC GAGGGTCCCC AGGGGCTCCA GGAGAGGCAG GTCGACCTGG CCTGCCAGGC
CCCGTGGGGC TGCCGGGCTT CTGTGAACCT GCCGCCTGCC TTGGAGCTTC GGCCTATGCC
TCTGCCCGCC TTACAGAGCC TGGATCCATC AAGGGGCCTT GA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

CloneID OHu83146
Clone ID Related Accession (Same CDS sequence) XM_017000333.1
Accession Version XM_017000333.1 Latest version! Documents for ORF clone product in dufault vector
Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
Protein sequence
SNP
Vector pcDNA3.1+/C-(K)DYK or customized vector User Manual
Clone information Clone Map MSDS
Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
ORF Insert Method CloneEZ® Seamless cloning technology
Insert Structure linear
Update Date 2016-06-06
Organism Homo sapiens(Human)
Product collagen alpha-2(IX) chain isoform X4
Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGTGT
CCAACCAACT GTCCACCCGG AATGAAAGGT CCCCCAGGGC TGCAGGGAGT GAAGGGGCAT
GCGGGCAAAC GCGGGATTCT GGGTGATCCT GGCCACCAGG GGAAGCCGGG TCCCAAGGGA
GATGTGGGTG CCTCTGGAGA GCAAGGCATC CCTGGACCAC CGGGTCCCCA GGGCATCAGG
GGCTACCCAG GCATGGCAGG GCCCAAGGGA GAGACGGGCC CTCATGGATA TAAAGGCATG
GTGGGCGCTA TCGGTGCCAC TGGGCCACCG GGTGAGGAAG GTCCTAGGGG ACCGCCAGGC
CGAGCTGGGG AGAAGGGTGA CGAGGGCAGC CCAGGTATTC GTGGACCCCA GGGGATCACA
GGCCCGAAAG GAGCAACGGG CCCCCCAGGC ATCAACGGCA AGGATGGGAC CCCAGGCACG
CCTGGCATGA AGGGCAGTGC AGGACAGGCG GGACAGCCCG GAAGTCCAGG CCACCAGGGC
CTAGCGGGTG TGCCAGGCCA GCCTGGGACA AAAGGAGGCC CTGGAGACCA GGGTGAGCCG
GGCCCGCAGG GCCTTCCTGG ATTCTCTGGT CCCCCTGGGA AAGAGGGAGA GCCAGGGCCT
CGAGGAGAAA TTGGTCCCCA GGGCATCATG GGACAGAAGG GTGACCAAGG CGAGAGGGGT
CCAGTGGGGC AACCAGGCCC TCAGGGAAGG CAGGGCCCTA AGGGGGAGCA GGGCCCCCCC
GGAATTCCAG GGCCCCAAGG CTTGCCAGGC GTCAAAGGAG ACAAGGGCTC CCCAGGGAAG
ACCGGGCCCC GCGGCAAAGT GGGTGACCCA GGGGTGGCCG GCCTCCCCGG AGAGAAAGGC
GAGAAGGGCG AGTCCGGCGA GCCGGGGCCC AAGGGACAGC AAGGAGTACG TGGAGAACCC
GGCTACCCTG GCCCCAGCGG GGATGCGGGC GCCCCAGGGG TTCAGGGCTA CCCTGGTCCC
CCCGGCCCTC GAGGACTGGC CGGGAACCGA GGCGTGCCAG GACAGCCCGG GAGACAGGGC
GTGGAGGGCC GGGATGCCAC TGACCAGCAC ATCGTGGATG TGGCGCTGAA GATGCTGCAA
GAGCAACTGG CAGAGGTCGC CGTGAGTGCC AAGCGGGAAG CCCTGGGTGC GGTGGGCATG
ATGGGTCCTC CAGGACCTCC TGGGCCCCCT GGGTACCCAG GCAAGCAGGG CCCCCATGGG
CACCCTGGCC CTCGGGGCGT TCCTGGCATC GTGGGAGCCG TGGGTCAGAT CGGCAACACG
GGGCCCAAGG GAAAACGTGG AGAGAAGGGT GATCCAGGAG AAGTGGGACG GGGGCACCCC
GGGATGCCTG GGCCCCCAGG GATCCCAGGA CTCCCTGGCC GGCCTGGCCA GGCAATCAAC
GGCAAGGATG GAGATCGAGG GTCCCCAGGG GCTCCAGGAG AGGCAGGTCG ACCTGGCCTG
CCAGGCCCCG TGGGGCTGCC GGGCTTCTGT GAACCTGCCG CCTGCCTTGG AGCTTCGGCC
TATGCCTCTG CCCGCCTTAC AGAGCCTGGA TCCATCAAGG GGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

RefSeq XP_016855822.1
CDS407..2194
Misc Feature(1)899..1072
Misc Feature(2)1106..1282
Misc Feature(3)1205..1366
Misc Feature(4)1400..1573
Translation

Target ORF information:

RefSeq Version XM_017000333.1
Organism Homo sapiens(Human)
Definition Homo sapiens collagen type IX alpha 2 (COL9A2), transcript variant X5, mRNA.

Target ORF information:

Epitope DYKDDDDK
Bacterial selection AMPR
Mammalian selection NeoR
Vector pcDNA3.1+/C-(K)DYK
XM_017000333.1

ORF Insert Sequence:

1
61
121
181
241
301
361
421
481
541
601
661
721
781
841
901
961
1021
1081
1141
1201
1261
1321
1381
1441
1501
1561
1621
1681
1741
ATGGGGATCC CTGGAGTCAA GGGCCAGCCC GGGCTTCCTG GTCCTCCTGG CCTTCCGGGC 
CCTGGTTTTG CTGGACCTCC TGGGCCTCCT GGACCTGTTG GCCTCCCTGG TGAGATTGGA
ATCCGAGGCC CCAAGGGGGA CCCTGGACCA GATGGACCAT CGGGGCCCCC AGGACCCCCT
GGGAAACCTG GTCGCCCGGG AACCATCCAG GGTCTGGAAG GCAGTGCGGA TTTCCTGTGT
CCAACCAACT GTCCACCCGG AATGAAAGGT CCCCCAGGGC TGCAGGGAGT GAAGGGGCAT
GCGGGCAAAC GCGGGATTCT GGGTGATCCT GGCCACCAGG GGAAGCCGGG TCCCAAGGGA
GATGTGGGTG CCTCTGGAGA GCAAGGCATC CCTGGACCAC CGGGTCCCCA GGGCATCAGG
GGCTACCCAG GCATGGCAGG GCCCAAGGGA GAGACGGGCC CTCATGGATA TAAAGGCATG
GTGGGCGCTA TCGGTGCCAC TGGGCCACCG GGTGAGGAAG GTCCTAGGGG ACCGCCAGGC
CGAGCTGGGG AGAAGGGTGA CGAGGGCAGC CCAGGTATTC GTGGACCCCA GGGGATCACA
GGCCCGAAAG GAGCAACGGG CCCCCCAGGC ATCAACGGCA AGGATGGGAC CCCAGGCACG
CCTGGCATGA AGGGCAGTGC AGGACAGGCG GGACAGCCCG GAAGTCCAGG CCACCAGGGC
CTAGCGGGTG TGCCAGGCCA GCCTGGGACA AAAGGAGGCC CTGGAGACCA GGGTGAGCCG
GGCCCGCAGG GCCTTCCTGG ATTCTCTGGT CCCCCTGGGA AAGAGGGAGA GCCAGGGCCT
CGAGGAGAAA TTGGTCCCCA GGGCATCATG GGACAGAAGG GTGACCAAGG CGAGAGGGGT
CCAGTGGGGC AACCAGGCCC TCAGGGAAGG CAGGGCCCTA AGGGGGAGCA GGGCCCCCCC
GGAATTCCAG GGCCCCAAGG CTTGCCAGGC GTCAAAGGAG ACAAGGGCTC CCCAGGGAAG
ACCGGGCCCC GCGGCAAAGT GGGTGACCCA GGGGTGGCCG GCCTCCCCGG AGAGAAAGGC
GAGAAGGGCG AGTCCGGCGA GCCGGGGCCC AAGGGACAGC AAGGAGTACG TGGAGAACCC
GGCTACCCTG GCCCCAGCGG GGATGCGGGC GCCCCAGGGG TTCAGGGCTA CCCTGGTCCC
CCCGGCCCTC GAGGACTGGC CGGGAACCGA GGCGTGCCAG GACAGCCCGG GAGACAGGGC
GTGGAGGGCC GGGATGCCAC TGACCAGCAC ATCGTGGATG TGGCGCTGAA GATGCTGCAA
GAGCAACTGG CAGAGGTCGC CGTGAGTGCC AAGCGGGAAG CCCTGGGTGC GGTGGGCATG
ATGGGTCCTC CAGGACCTCC TGGGCCCCCT GGGTACCCAG GCAAGCAGGG CCCCCATGGG
CACCCTGGCC CTCGGGGCGT TCCTGGCATC GTGGGAGCCG TGGGTCAGAT CGGCAACACG
GGGCCCAAGG GAAAACGTGG AGAGAAGGGT GATCCAGGAG AAGTGGGACG GGGGCACCCC
GGGATGCCTG GGCCCCCAGG GATCCCAGGA CTCCCTGGCC GGCCTGGCCA GGCAATCAAC
GGCAAGGATG GAGATCGAGG GTCCCCAGGG GCTCCAGGAG AGGCAGGTCG ACCTGGCCTG
CCAGGCCCCG TGGGGCTGCC GGGCTTCTGT GAACCTGCCG CCTGCCTTGG AGCTTCGGCC
TATGCCTCTG CCCGCCTTAC AGAGCCTGGA TCCATCAAGG GGCCTTGA

The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

book

Association between COL9A2 Gln326Arg mutations and the development of intervertebral disc disease in a Chinese population.
Genetics and molecular research : GMR15(4)(2016 Dec)
Meng T,Ren Q,Wang JM,Shi H,Zhang ST,Liu MT


book

Meta-analysis of the association between COL9A2 genetic polymorphisms and lumbar disc disease susceptibility.
Spine39(20)1699-706(2014 Sep)
Zhang Z,Zhang J,Ding L,Teng X


book

[Analysis of COL9A2 gene mutations in a Chinese Han population with pathological myopia].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics31(2)129-33(2014 Apr)
Chen R,Gong B,Li Q,Zeng G,Hao F,Li N,Shi Y,Zhang D


book

The role of polymorphisms of genes encoding collagen IX and XI in lumbar disc disease.
Neurologia i neurochirurgia polska48(1)60-2(2014 Jan-Feb)
Janeczko Ł,Janeczko M,Chrzanowski R,Zieliński G


 
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