CACUL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACUL1
Entrez Gene ID	143384
Full Name	CDK2 associated cullin domain 1
Synonyms	C10orf46,CAC1
General protein information	Preferred Names CDK2 associated cullin domain 1 Names CDK2-associated and cullin domain-containing protein 1 Cdk-Associated Cullin1 cullin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q26.11

mRNA and Protein(s)

mRNA	Protein	Name
NM_153810.5	NP_722517.3	CDK2-associated and cullin domain-containing protein 1
NM_153810.4	NP_722517.3	CDK2-associated and cullin domain-containing protein 1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Cacul1	NP_001014270.1
Gallus gallus (chicken)	C6H10ORF46	NP_001026409.1
Canis lupus familiaris (dog)	CACUL1	XP_535028.3
Bos taurus (cattle)	CACUL1	XP_002698587.2
Mus musculus (house mouse)	Cacul1	NP_084473.1
Pan troglodytes (chimpanzee)	CACUL1	XP_001153044.1
	CACUL1	XP_001096592.1
Danio rerio (zebrafish)	LOC100538081	XP_003199807.2
Xenopus tropicalis (tropical clawed frog)	cacul1	XP_004915792.1
Homo sapiens (human)	CACUL1	NP_722517.3

Gene Ontology
Bibliography

Related articles in PubMed

CACUL1/CAC1 attenuates p53 activity through PML post-translational modification.
Fukuda T, Kigoshi-Tansho Y, Naganuma T, Kazaana A, Okajima T, Tsuruta F, Chiba T
Biochemical and biophysical research communications482(4)863-869(2017 Jan)

CACUL1 functions as a negative regulator of androgen receptor in prostate cancer cells.
Choi H, Lee SH, Um SJ, Kim EJ
Cancer letters376(2)360-6(2016 07)

miR-106a* inhibits the proliferation of esophageal carcinoma cells by targeting CDK2-associated Cullin 1 (CACUL1).
Ma HL, Wen XP, Zhang XZ, Wang XL, Zhao DL, Che SM, Dang CX
Cellular and molecular biology (Noisy-le-Grand, France)61(4)56-62(2015 Aug)

Helicobacter pylori promotes invasion and metastasis of gastric cancer cells through activation of AP-1 and up-regulation of CACUL1.
Kong Y, Ma LQ, Bai PS, Da R, Sun H, Qi XG, Ma JQ, Zhao RM, Chen NZ, Nan KJ
The international journal of biochemistry & cell biology45(11)2666-78(2013 Nov)

CDK-associated Cullin 1 promotes cell proliferation with activation of ERK1/2 in human lung cancer A549 cells.
Chen TJ, Gao F, Yang T, Thakur A, Ren H, Li Y, Zhang S, Wang T, Chen MW
Biochemical and biophysical research communications437(1)108-13(2013 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These data suggest that CACUL1 is a novel regulator that negatively controls p53 activity through the regulation of PML SUMOylation.
Title: CACUL1/CAC1 attenuates p53 activity through PML post-translational modification.

Suggest that CACUL1 impairs LSD1-mediated activation of AR, thereby implicating it as a potential antitumor target in prostate cancer cells.
Title: CACUL1 functions as a negative regulator of androgen receptor in prostate cancer cells.

silencing CACUL1 resulted in the same effects of miR-106a* overexpression in esophageal carcinoma cells, including suppressed esophageal carcinoma cell proliferation and blocked G1/S transition through CDK2 pathway by inhibiting cell cycle regulators.
Title: miR-106a* inhibits the proliferation of esophageal carcinoma cells by targeting CDK2-associated Cullin 1 (CACUL1).

H. pylori-triggered CACUL1 production occurred in an activating protein 1-dependent manner and regulated matrix metalloproteinase 9 and Slug expression to affect the invasion and metastasis of tumor cells.
Title: Helicobacter pylori promotes invasion and metastasis of gastric cancer cells through activation of AP-1 and up-regulation of CACUL1.

CAC1 promoted the proliferation of human lung cancer A549 cells with activation of ERK1/2 signaling pathways.
Title: CDK-associated Cullin 1 promotes cell proliferation with activation of ERK1/2 in human lung cancer A549 cells.

The following CACUL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACUL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07069	NM_153810.5 Latest version!	Homo sapiens CDK2 associated cullin domain 1 (CACUL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu07069	NM_153810.4	Homo sapiens CDK2 associated cullin domain 1 (CACUL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACUL1 ( NM_153810.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_722517.3 Homo sapiens CDK2 associated cullin domain 1 (CACUL1), mRNA.

Price & Availability↑

CloneID	OHu07069
Clone ID Related Accession (Same CDS sequence)	NM_153810.5 , NM_153810.4
Accession Version	NM_153810.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	CDK2-associated and cullin domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AJ544771.1, AY743663.1, AL139407.11 and BU623607.1. On Nov 22, 2018 this sequence version replaced NM_153810.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.18020.1, AY743663.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000369151.8/ ENSP00000358147.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGAAA GCATGGAAGA GGAGGAGGGG GGCAGCTACG AGGCGATGAT GGACGACCAG 
AACCACAACA ACTGGGAGGC TGCGGTGGAC GGCTTCCGGC AGCCCCTGCC ACCTCCGCCG 
CCCCCCTCGT CGATCCCGGC CCCTGCCCGA GAGCCTCCGG GGGGGCAGCT GCTGGCGGTG 
CCCGCGGTCT CCGTGGACAG GAAAGGCCCC AAGGAGGGGC TCCCGATGGG GCCGCAGCCA 
CCGCCGGAGG CTAATGGGGT GATCATGATG TTGAAGAGCT GCGACGCGGC CGCCGCCGTG 
GCCAAGGCGG CCCCCGCCCC CACCGCCAGC TCCACCATCA ACATCAACAC CTCCACCTCC 
AAGTTCTTAA TGAATGTTAT AACTATTGAA GATTATAAGA GCACATACTG GCCAAAATTG 
GATGGTGCCA TAGATCAACT TTTAACTCAG AGTCCTGGTG ACTATATCCC CATATCCTAT 
GAACAGATAT ACAGTTGTGT GTATAAATGT GTATGCCAGC AGCACTCGGA ACAGATGTAT 
AGTGATCTGA TTAAAAAGAT AACTAATCAC TTAGAGAGAG TCTCAAAGGA GCTGCAGGCC 
AGCCCTCCAG ATCTCTATAT TGAAAGATTT AATATAGCTC TTGGACAATA TATGGGAGCA 
TTGCAGAGCA TTGTGCCTCT TTTCATATAT ATGAATAAGT TTTACATCGA AACCAAGCTT 
AACAGAGACT TAAAAGATGA CCTTATAAAG CTGTTTACGG AACATGTTGC AGAAAAGCAC 
ATTTACAGCC TAATGCCTTT ACTTTTAGAA GCCCAGTCAA CACCATTTCA GGTCACACCT 
TCAACTATGG CAAATATTGT GAAAGGCCTG TATACCCTCA GACCAGAGTG GGTTCAGATG 
GCTCCAACTC TATTTTCTAA ATTTATTCCA AACATTCTCC CTCCGGCGGT GGAATCTGAA 
CTTTCTGAAT ATGCTGCTCA AGATCAGAAA TTTCAAAGAG AACTTATACA GAATGGTTTT 
ACAAGGGGTG ACCAGTCCCG GAAGAGAGCT GGGGATGAGT TGGCTTATAA TAGCTCGTCA 
GCATGTGCAA GTTCCAGGGG GTACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722517.3
CDS	209..1318
Translation	MEESMEEEEGGSYEAMMDDQNHNNWEAAVDGFRQPLPPPPPPSSIPAPAREPPGGQLLAVPAVSVDRKGPKEGLPMGPQPPPEANGVIMMLKSCDAAAAVAKAAPAPTASSTININTSTSKFLMNVITIEDYKSTYWPKLDGAIDQLLTQSPGDYIPISYEQIYSCVYKCVCQQHSEQMYSDLIKKITNHLERVSKELQASPPDLYIERFNIALGQYMGALQSIVPLFIYMNKFYIETKLNRDLKDDLIKLFTEHVAEKHIYSLMPLLLEAQSTPFQVTPSTMANIVKGLYTLRPEWVQMAPTLFSKFIPNILPPAVESELSEYAAQDQKFQRELIQNGFTRGDQSRKRAGDELAYNSSSACASSRGYR

Target ORF information:

RefSeq Version	NM_153810.5
Organism	Homo sapiens(human)
Definition	Homo sapiens CDK2 associated cullin domain 1 (CACUL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153810.5

ORF Insert Sequence:

ATGGAGGAAA GCATGGAAGA GGAGGAGGGG GGCAGCTACG AGGCGATGAT GGACGACCAG 
AACCACAACA ACTGGGAGGC TGCGGTGGAC GGCTTCCGGC AGCCCCTGCC ACCTCCGCCG 
CCCCCCTCGT CGATCCCGGC CCCTGCCCGA GAGCCTCCGG GGGGGCAGCT GCTGGCGGTG 
CCCGCGGTCT CCGTGGACAG GAAAGGCCCC AAGGAGGGGC TCCCGATGGG GCCGCAGCCA 
CCGCCGGAGG CTAATGGGGT GATCATGATG TTGAAGAGCT GCGACGCGGC CGCCGCCGTG 
GCCAAGGCGG CCCCCGCCCC CACCGCCAGC TCCACCATCA ACATCAACAC CTCCACCTCC 
AAGTTCTTAA TGAATGTTAT AACTATTGAA GATTATAAGA GCACATACTG GCCAAAATTG 
GATGGTGCCA TAGATCAACT TTTAACTCAG AGTCCTGGTG ACTATATCCC CATATCCTAT 
GAACAGATAT ACAGTTGTGT GTATAAATGT GTATGCCAGC AGCACTCGGA ACAGATGTAT 
AGTGATCTGA TTAAAAAGAT AACTAATCAC TTAGAGAGAG TCTCAAAGGA GCTGCAGGCC 
AGCCCTCCAG ATCTCTATAT TGAAAGATTT AATATAGCTC TTGGACAATA TATGGGAGCA 
TTGCAGAGCA TTGTGCCTCT TTTCATATAT ATGAATAAGT TTTACATCGA AACCAAGCTT 
AACAGAGACT TAAAAGATGA CCTTATAAAG CTGTTTACGG AACATGTTGC AGAAAAGCAC 
ATTTACAGCC TAATGCCTTT ACTTTTAGAA GCCCAGTCAA CACCATTTCA GGTCACACCT 
TCAACTATGG CAAATATTGT GAAAGGCCTG TATACCCTCA GACCAGAGTG GGTTCAGATG 
GCTCCAACTC TATTTTCTAA ATTTATTCCA AACATTCTCC CTCCGGCGGT GGAATCTGAA 
CTTTCTGAAT ATGCTGCTCA AGATCAGAAA TTTCAAAGAG AACTTATACA GAATGGTTTT 
ACAAGGGGTG ACCAGTCCCG GAAGAGAGCT GGGGATGAGT TGGCTTATAA TAGCTCGTCA 
GCATGTGCAA GTTCCAGGGG GTACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACUL1 ( NM_153810.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_722517.3 Homo sapiens CDK2 associated cullin domain 1 (CACUL1), mRNA.

Price & Availability↑

CloneID	OHu07069
Clone ID Related Accession (Same CDS sequence)	NM_153810.5 , NM_153810.4
Accession Version	NM_153810.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1110bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	CDK2-associated and cullin domain-containing protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG772719.1, AJ544771.1, AY743663.1, AL139407.11 and BU623607.1. On Jun 22, 2006 this sequence version replaced NM_153810.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY743663.1, SRR1660807.145654.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAAA GCATGGAAGA GGAGGAGGGG GGCAGCTACG AGGCGATGAT GGACGACCAG 
AACCACAACA ACTGGGAGGC TGCGGTGGAC GGCTTCCGGC AGCCCCTGCC ACCTCCGCCG 
CCCCCCTCGT CGATCCCGGC CCCTGCCCGA GAGCCTCCGG GGGGGCAGCT GCTGGCGGTG 
CCCGCGGTCT CCGTGGACAG GAAAGGCCCC AAGGAGGGGC TCCCGATGGG GCCGCAGCCA 
CCGCCGGAGG CTAATGGGGT GATCATGATG TTGAAGAGCT GCGACGCGGC CGCCGCCGTG 
GCCAAGGCGG CCCCCGCCCC CACCGCCAGC TCCACCATCA ACATCAACAC CTCCACCTCC 
AAGTTCTTAA TGAATGTTAT AACTATTGAA GATTATAAGA GCACATACTG GCCAAAATTG 
GATGGTGCCA TAGATCAACT TTTAACTCAG AGTCCTGGTG ACTATATCCC CATATCCTAT 
GAACAGATAT ACAGTTGTGT GTATAAATGT GTATGCCAGC AGCACTCGGA ACAGATGTAT 
AGTGATCTGA TTAAAAAGAT AACTAATCAC TTAGAGAGAG TCTCAAAGGA GCTGCAGGCC 
AGCCCTCCAG ATCTCTATAT TGAAAGATTT AATATAGCTC TTGGACAATA TATGGGAGCA 
TTGCAGAGCA TTGTGCCTCT TTTCATATAT ATGAATAAGT TTTACATCGA AACCAAGCTT 
AACAGAGACT TAAAAGATGA CCTTATAAAG CTGTTTACGG AACATGTTGC AGAAAAGCAC 
ATTTACAGCC TAATGCCTTT ACTTTTAGAA GCCCAGTCAA CACCATTTCA GGTCACACCT 
TCAACTATGG CAAATATTGT GAAAGGCCTG TATACCCTCA GACCAGAGTG GGTTCAGATG 
GCTCCAACTC TATTTTCTAA ATTTATTCCA AACATTCTCC CTCCGGCGGT GGAATCTGAA 
CTTTCTGAAT ATGCTGCTCA AGATCAGAAA TTTCAAAGAG AACTTATACA GAATGGTTTT 
ACAAGGGGTG ACCAGTCCCG GAAGAGAGCT GGGGATGAGT TGGCTTATAA TAGCTCGTCA 
GCATGTGCAA GTTCCAGGGG GTACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_722517.3
CDS	485..1594
Translation	MEESMEEEEGGSYEAMMDDQNHNNWEAAVDGFRQPLPPPPPPSSIPAPAREPPGGQLLAVPAVSVDRKGPKEGLPMGPQPPPEANGVIMMLKSCDAAAAVAKAAPAPTASSTININTSTSKFLMNVITIEDYKSTYWPKLDGAIDQLLTQSPGDYIPISYEQIYSCVYKCVCQQHSEQMYSDLIKKITNHLERVSKELQASPPDLYIERFNIALGQYMGALQSIVPLFIYMNKFYIETKLNRDLKDDLIKLFTEHVAEKHIYSLMPLLLEAQSTPFQVTPSTMANIVKGLYTLRPEWVQMAPTLFSKFIPNILPPAVESELSEYAAQDQKFQRELIQNGFTRGDQSRKRAGDELAYNSSSACASSRGYR

Target ORF information:

RefSeq Version	NM_153810.4
Organism	Homo sapiens(human)
Definition	Homo sapiens CDK2 associated cullin domain 1 (CACUL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153810.4

ORF Insert Sequence:

ATGGAGGAAA GCATGGAAGA GGAGGAGGGG GGCAGCTACG AGGCGATGAT GGACGACCAG 
AACCACAACA ACTGGGAGGC TGCGGTGGAC GGCTTCCGGC AGCCCCTGCC ACCTCCGCCG 
CCCCCCTCGT CGATCCCGGC CCCTGCCCGA GAGCCTCCGG GGGGGCAGCT GCTGGCGGTG 
CCCGCGGTCT CCGTGGACAG GAAAGGCCCC AAGGAGGGGC TCCCGATGGG GCCGCAGCCA 
CCGCCGGAGG CTAATGGGGT GATCATGATG TTGAAGAGCT GCGACGCGGC CGCCGCCGTG 
GCCAAGGCGG CCCCCGCCCC CACCGCCAGC TCCACCATCA ACATCAACAC CTCCACCTCC 
AAGTTCTTAA TGAATGTTAT AACTATTGAA GATTATAAGA GCACATACTG GCCAAAATTG 
GATGGTGCCA TAGATCAACT TTTAACTCAG AGTCCTGGTG ACTATATCCC CATATCCTAT 
GAACAGATAT ACAGTTGTGT GTATAAATGT GTATGCCAGC AGCACTCGGA ACAGATGTAT 
AGTGATCTGA TTAAAAAGAT AACTAATCAC TTAGAGAGAG TCTCAAAGGA GCTGCAGGCC 
AGCCCTCCAG ATCTCTATAT TGAAAGATTT AATATAGCTC TTGGACAATA TATGGGAGCA 
TTGCAGAGCA TTGTGCCTCT TTTCATATAT ATGAATAAGT TTTACATCGA AACCAAGCTT 
AACAGAGACT TAAAAGATGA CCTTATAAAG CTGTTTACGG AACATGTTGC AGAAAAGCAC 
ATTTACAGCC TAATGCCTTT ACTTTTAGAA GCCCAGTCAA CACCATTTCA GGTCACACCT 
TCAACTATGG CAAATATTGT GAAAGGCCTG TATACCCTCA GACCAGAGTG GGTTCAGATG 
GCTCCAACTC TATTTTCTAA ATTTATTCCA AACATTCTCC CTCCGGCGGT GGAATCTGAA 
CTTTCTGAAT ATGCTGCTCA AGATCAGAAA TTTCAAAGAG AACTTATACA GAATGGTTTT 
ACAAGGGGTG ACCAGTCCCG GAAGAGAGCT GGGGATGAGT TGGCTTATAA TAGCTCGTCA 
GCATGTGCAA GTTCCAGGGG GTACAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CACUL1/CAC1 attenuates p53 activity through PML post-translational modification.
Biochemical and biophysical research communications482(4)863-869(2017 Jan)
Fukuda T,Kigoshi-Tansho Y,Naganuma T,Kazaana A,Okajima T,Tsuruta F,Chiba T

CACUL1 functions as a negative regulator of androgen receptor in prostate cancer cells.
Cancer letters376(2)360-6(2016 07)
Choi H,Lee SH,Um SJ,Kim EJ

miR-106a* inhibits the proliferation of esophageal carcinoma cells by targeting CDK2-associated Cullin 1 (CACUL1).
Cellular and molecular biology (Noisy-le-Grand, France)61(4)56-62(2015 Aug)
Ma HL,Wen XP,Zhang XZ,Wang XL,Zhao DL,Che SM,Dang CX

Helicobacter pylori promotes invasion and metastasis of gastric cancer cells through activation of AP-1 and up-regulation of CACUL1.
The international journal of biochemistry & cell biology45(11)2666-78(2013 Nov)
Kong Y,Ma LQ,Bai PS,Da R,Sun H,Qi XG,Ma JQ,Zhao RM,Chen NZ,Nan KJ

CDK-associated Cullin 1 promotes cell proliferation with activation of ERK1/2 in human lung cancer A549 cells.
Biochemical and biophysical research communications437(1)108-13(2013 Jul)
Chen TJ,Gao F,Yang T,Thakur A,Ren H,Li Y,Zhang S,Wang T,Chen MW

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CACUL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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