Hectd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

  • Gene

  • Clones

  • gRNAs

The following Hectd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hectd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa55591 XM_017603165.1
Latest version!
Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
ORa29336 XM_006240086.2
Latest version!
Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa29337 XM_006240087.2
Latest version!
Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa29338 XM_006240088.2
Latest version!
Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
ORa29339 XM_017594442.1
Latest version!
Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa55591
    Clone ID Related Accession (Same CDS sequence) XM_017603165.1
    Accession Version XM_017603165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000074.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGAGC
    TCTGTGTGGC AGGCAGGGCT TCATGGAATA GGTGGTCACC GTGCTATTTT AACCCTTTGT
    AGGGAAGTCA CTAACATACT TTTTGTCAAA TGCATTTCCA GGTCAACTGC AACGTGGCCA
    CTTGACCCAG CAAAGGATGA AAAACAAGGA TGGCGGCATG TGAGGCTGAA GCAGATGGGA
    AAGAACGCAA GCGGACAGAC ACACTACCTC TCCCTCTCTG GCTTTGAGCT CTATGGCACT
    GTAAATGGCG TGTGTGAAGA CCAACTCGGG AAAGCAGCTA AAGAAGCAGA AGCTAATCTT
    AGAAGGCAGA GGCGCCTGGT GCGTTCTCAA GTCCTGAAGT ACATGGTTCC AGGAGCACGT
    GTTATCAGAG GCCTTGACTG GAAGTGGCGA GATCAGGATG GCAGCCCACA GGGAGAAGGC
    ACAGTCACAG GAGAGCTGCA CAATGGCTGG ATTGATGTCA CCTGGGATGC TGGTGGCTCA
    AACTCTTACC GTATGGGCGC AGAAGGAAAA TTTGACCTCA AGCTTGCACC AGGGTACGAC
    CCTGATACAG TGGCATCACC CAAACCTGTT TCATCCACTG TTTCAGGCAC AACGCAGTCA
    TGGAGCAGCT TGGTGAAAAA CAACTGTCCA GACAAGACGT CTGCTGCTGC AGGCTCCTCA
    AGTAGAAAAG GAAGCAGCAG TTCTGTGTGT AGCGTGGCCA GTAGCAGCGA CATCAGCTTG
    GGTTCGACCA AAACGGAACG GAGATCAGAA ATTGTAATGG AACACAGTAT AGTTTCAGGA
    GCTGATGTCC ATGAACCAAT TGTTGTTCTT TCATCTGCTG AAAACGTTCC TCAAACAGAA
    GTAGGGTCAT CTTCCAGTGC AAGCACCAGC ACCCTAACAG CGGAAACGGG AAGTGAAAAT
    GCTGAAAGGA AGTTAGGCCC GGATAGTTCT GTTCGAGCTC CTGGGGAGTC TAGTGCAATA
    TCCATGGGAA TTGTTAGCGT TAGCTCTCCT GATGTTAGTT CAGTATCTGA ATTAACTAAT
    AAGGAAGCAG CTTCACAACG ACCCCTTAGC TCGTCAGCAA GTAACAGACT GTCAGTGAGC
    TCTCTGTTGG CAGCTGGGGC CCCTATGAGC TCTAGTGCAA GTGTACCTAA CCTGTCCTCA
    AGAGAAACAT CTAGCTTGGA GAGCTTTGTA AGGAGAGTGG CAAACATAGC ACGGACTAAT
    GCCACCAACA ACATGAACCT GAGCCGGAGC AGCAGTGATA ACAACACTAA CACTCTGGGG
    CGGAATGTGA TGAGCACCGC AACTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC GACAGTGACA ATGTCAACAT CAAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CGGCGACAAC AGTTTTATCA GTGGGTCAGT CATTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC TAGTGAGAGT GACACAGGGC AGGAAGCAGA ATATTCCTTA
    TATGACTTTC TTGATAGCTG TCGTGCCAGT ACTCTCTTAG CTGAGCTAGA TGATGACGAG
    GACTTGCCTG AACCGGATGA GGAAGACGAT GAGAACGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCATCTTTGC AACGTCGTGC TGGCTCTCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCTTC TGGAGCAGGG
    AGCCGACCTA TTGGGGAACA GGAAGAAGAA GAGTATGAAA CTAAAGGAGG CCGCCGGAGA
    ACCTGGGATG ACGACTACGT GCTGAAGCGG CAGTTCTCTG CACTGGTTCC CGCTTTTGAT
    CCTAGGCCTG GTCGTACCAA TGTCCAACAG ACAACTGACC TAGAAATCCC TCCCCCAGGA
    ACACCTCACT CAGAGCTCTT GGAGGAAGTC GAGTGTACTC CATCACCTCG ATTGGCTCTC
    ACGTTGAAAG TGACTGGGCT TGGAACGACT CGGGAAGTTG AACTGCCACT TAGCAACTTC
    AGATCAACCA TCTTTTATTA TGTACAGAAA CTGCTTCAGC TGTCTTGTAA TGGCAATGTG
    AAGTCAGACA AACTTAGGCG TATTTGGGAG CCAACTTACA CAATCATGTA TAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGG AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCTGACG CCGCTTTCCT TCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTTATAGCC GCATATAAGG ATTTTTGTGA GCATGGAACA
    AGGTCTGGAT TAAACCAGGG GGCTAGTTCT AGTCTTCAAA GCAGTGATAT TCTTAATTTG
    ACAAAAGAGC AGCCTCAGGC TAAAGCCGGC AACGGACAGA ACCCCTGTGG AGTAGAGGAC
    GTTCTTCAGC TTCTGCGCAT TCTGTATATA GTTGCAAGTG ACCCTTATTC AAGGATATCC
    CAAGAAGATG GTGATGAGCA GCCCCAGTTT ACTTTTCCAC CTGATGAATT TACTAGTAAA
    AAGATCACCA CAAAAATTTT GCAGCAGATT GAGGAGCCAT TGGCTTTGGC AAGCGGGGCT
    CTGCCAGACT GGTGTGAACA GCTGACCAGC AAGTGTCCTT TCCTAATCCC ATTCGAAACC
    AGACAGCTTT ACTTCACCTG TACTGCGTTT GGTGCATCCA GAGCAATAGT GTGGCTACAG
    AATCGACGGG AAGCCACAGT GGAGCGAACA AGGACCACGA GCAGTGTAAG GCGAGATGAT
    CCTGGGGAAT TTAGAGTTGG CCGACTCAAG CACGAAAGAG TGAAGGTTCC CCGTGGAGAG
    TCTCTGATGG AGTGGGCTGA GAATGTCATG CAAATACACG CAGACCGGAA GTCAGTTCTC
    GAGGTTGAAT TTCTAGGAGA AGAAGGAACT GGCCTGGGAC CCACTTTAGA GTTTTATGCT
    CTTGTGGCAG CAGAATTCCA GAGGACCGAC TTGGGGACTT GGCTTTGTGA TGATAACTTT
    CCAGATGATG AATCTCGTCA TGTTGACCTT GGAGGTGGGT TGAAGCCTCC TGGATACTAT
    GTACAGCGAT CATGTGGACT GTTCACAGCG CCATTTCCAC AAGACAGTGA TGAGCTGGAA
    AGGATCACAA AACTCTTCCA TTTCCTTGGG ATTTTCTTGG CCAAATGTAT TCAAGACAAT
    AGATTGGTGG ACTTGCCTAT TTCTAAGCCT TTCTTTAAAC TTATGTGCAT GGGCGACATT
    AAAAGCAATA TGAGCAAACT GATCTATGAA TCACGAGGCG ACAGAGACTT GCACTGCACT
    GAAAGTCAGT CTGAAGCGTC TACGGAAGAA GGTCATGACT CCCTCTCAGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATCCTT GATCCCCCTA AGCCCAAACC CCCGGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGATTTT GAACTAGTAA ATCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATTAAG AGGCGCCAGA TTTTAGGCAA TAAAAGCCTT
    TCCGAAGATG AGAAGAACAC AAAGTTACAG GAACTAGTAC TCAAGAACCC ATCAGGTTCT
    GGACCTCCAC TTAGCATAGA GGACTTAGGC TTAAATTTCC AGTTTTGCCC ATCTTCCAGA
    ATATATGGCT TCACAGCGGT GGACCTCAAG CCAAGTGGAG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATCCAG
    AAACAGATGG AAGCTTTTCG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAGGAAGT CCAGATGATT CTTTGTGGAA ATCAATCACC ATCCTGGGCA
    GCAGAGGACA TTATCAATTA TACTGAACCT AAGCTTGGCT ATACACGTGA CAGTCCCGGC
    TTCCTTAGGT TTGTGAGGGT CTTATGTGGC ATGTCCTCAG ACGAACGGAA AGCATTCCTG
    CAGTTTACCA CCGGCTGTTC AACTCTGCCC CCAGGTGGAC TGGCTAACCT GCATCCGAGG
    CTCACAGTCG TGCGAAAGGT TGATGCTACT GATGCAAGCT ATCCATCCGT CAACACTTGT
    GTCCACTATC TTAAGTTACC TGAATATTCT TCTGAGGAGA TCATGAGAGA GCGCTTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017458654.1
    CDS391..7866
    Translation

    Target ORF information:

    RefSeq Version XM_017603165.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017603165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTGAGC
    TCTGTGTGGC AGGCAGGGCT TCATGGAATA GGTGGTCACC GTGCTATTTT AACCCTTTGT
    AGGGAAGTCA CTAACATACT TTTTGTCAAA TGCATTTCCA GGTCAACTGC AACGTGGCCA
    CTTGACCCAG CAAAGGATGA AAAACAAGGA TGGCGGCATG TGAGGCTGAA GCAGATGGGA
    AAGAACGCAA GCGGACAGAC ACACTACCTC TCCCTCTCTG GCTTTGAGCT CTATGGCACT
    GTAAATGGCG TGTGTGAAGA CCAACTCGGG AAAGCAGCTA AAGAAGCAGA AGCTAATCTT
    AGAAGGCAGA GGCGCCTGGT GCGTTCTCAA GTCCTGAAGT ACATGGTTCC AGGAGCACGT
    GTTATCAGAG GCCTTGACTG GAAGTGGCGA GATCAGGATG GCAGCCCACA GGGAGAAGGC
    ACAGTCACAG GAGAGCTGCA CAATGGCTGG ATTGATGTCA CCTGGGATGC TGGTGGCTCA
    AACTCTTACC GTATGGGCGC AGAAGGAAAA TTTGACCTCA AGCTTGCACC AGGGTACGAC
    CCTGATACAG TGGCATCACC CAAACCTGTT TCATCCACTG TTTCAGGCAC AACGCAGTCA
    TGGAGCAGCT TGGTGAAAAA CAACTGTCCA GACAAGACGT CTGCTGCTGC AGGCTCCTCA
    AGTAGAAAAG GAAGCAGCAG TTCTGTGTGT AGCGTGGCCA GTAGCAGCGA CATCAGCTTG
    GGTTCGACCA AAACGGAACG GAGATCAGAA ATTGTAATGG AACACAGTAT AGTTTCAGGA
    GCTGATGTCC ATGAACCAAT TGTTGTTCTT TCATCTGCTG AAAACGTTCC TCAAACAGAA
    GTAGGGTCAT CTTCCAGTGC AAGCACCAGC ACCCTAACAG CGGAAACGGG AAGTGAAAAT
    GCTGAAAGGA AGTTAGGCCC GGATAGTTCT GTTCGAGCTC CTGGGGAGTC TAGTGCAATA
    TCCATGGGAA TTGTTAGCGT TAGCTCTCCT GATGTTAGTT CAGTATCTGA ATTAACTAAT
    AAGGAAGCAG CTTCACAACG ACCCCTTAGC TCGTCAGCAA GTAACAGACT GTCAGTGAGC
    TCTCTGTTGG CAGCTGGGGC CCCTATGAGC TCTAGTGCAA GTGTACCTAA CCTGTCCTCA
    AGAGAAACAT CTAGCTTGGA GAGCTTTGTA AGGAGAGTGG CAAACATAGC ACGGACTAAT
    GCCACCAACA ACATGAACCT GAGCCGGAGC AGCAGTGATA ACAACACTAA CACTCTGGGG
    CGGAATGTGA TGAGCACCGC AACTTCTCCT CTTATGGGTG CTCAGAGTTT CCCTAATTTG
    ACCACACCTG GTACTACATC GACAGTGACA ATGTCAACAT CAAGTGTTAC TAGCAGCAGC
    AATGTAGCTA CGGCGACAAC AGTTTTATCA GTGGGTCAGT CATTAAGTAA TACTTTAACC
    ACCAGCCTCA CATCAACCTC TAGTGAGAGT GACACAGGGC AGGAAGCAGA ATATTCCTTA
    TATGACTTTC TTGATAGCTG TCGTGCCAGT ACTCTCTTAG CTGAGCTAGA TGATGACGAG
    GACTTGCCTG AACCGGATGA GGAAGACGAT GAGAACGAAG ATGACAATCA GGAGGACCAA
    GAATACGAGG AGGTTATGAT TCTGAGACGT CCATCTTTGC AACGTCGTGC TGGCTCTCGC
    TCTGATGTAA CACACCATGC TGTCACCTCA CAGCTACCAC AGGTCCCTTC TGGAGCAGGG
    AGCCGACCTA TTGGGGAACA GGAAGAAGAA GAGTATGAAA CTAAAGGAGG CCGCCGGAGA
    ACCTGGGATG ACGACTACGT GCTGAAGCGG CAGTTCTCTG CACTGGTTCC CGCTTTTGAT
    CCTAGGCCTG GTCGTACCAA TGTCCAACAG ACAACTGACC TAGAAATCCC TCCCCCAGGA
    ACACCTCACT CAGAGCTCTT GGAGGAAGTC GAGTGTACTC CATCACCTCG ATTGGCTCTC
    ACGTTGAAAG TGACTGGGCT TGGAACGACT CGGGAAGTTG AACTGCCACT TAGCAACTTC
    AGATCAACCA TCTTTTATTA TGTACAGAAA CTGCTTCAGC TGTCTTGTAA TGGCAATGTG
    AAGTCAGACA AACTTAGGCG TATTTGGGAG CCAACTTACA CAATCATGTA TAGAGAAATG
    AAGGATTCTG ATAAAGAAAA GGAAAATGGG AAAATGGGTT GCTGGTCTAT AGAGCATGTG
    GAACAGTACC TTGGCACTGA TGAATTACCA AAGAATGACT TGATAACCTA CCTGCAGAAG
    AATGCTGACG CCGCTTTCCT TCGCCACTGG AAATTAACTG GCACTAATAA AAGTATTAGG
    AAAAACAGAA ATTGTTCTCA GCTTATAGCC GCATATAAGG ATTTTTGTGA GCATGGAACA
    AGGTCTGGAT TAAACCAGGG GGCTAGTTCT AGTCTTCAAA GCAGTGATAT TCTTAATTTG
    ACAAAAGAGC AGCCTCAGGC TAAAGCCGGC AACGGACAGA ACCCCTGTGG AGTAGAGGAC
    GTTCTTCAGC TTCTGCGCAT TCTGTATATA GTTGCAAGTG ACCCTTATTC AAGGATATCC
    CAAGAAGATG GTGATGAGCA GCCCCAGTTT ACTTTTCCAC CTGATGAATT TACTAGTAAA
    AAGATCACCA CAAAAATTTT GCAGCAGATT GAGGAGCCAT TGGCTTTGGC AAGCGGGGCT
    CTGCCAGACT GGTGTGAACA GCTGACCAGC AAGTGTCCTT TCCTAATCCC ATTCGAAACC
    AGACAGCTTT ACTTCACCTG TACTGCGTTT GGTGCATCCA GAGCAATAGT GTGGCTACAG
    AATCGACGGG AAGCCACAGT GGAGCGAACA AGGACCACGA GCAGTGTAAG GCGAGATGAT
    CCTGGGGAAT TTAGAGTTGG CCGACTCAAG CACGAAAGAG TGAAGGTTCC CCGTGGAGAG
    TCTCTGATGG AGTGGGCTGA GAATGTCATG CAAATACACG CAGACCGGAA GTCAGTTCTC
    GAGGTTGAAT TTCTAGGAGA AGAAGGAACT GGCCTGGGAC CCACTTTAGA GTTTTATGCT
    CTTGTGGCAG CAGAATTCCA GAGGACCGAC TTGGGGACTT GGCTTTGTGA TGATAACTTT
    CCAGATGATG AATCTCGTCA TGTTGACCTT GGAGGTGGGT TGAAGCCTCC TGGATACTAT
    GTACAGCGAT CATGTGGACT GTTCACAGCG CCATTTCCAC AAGACAGTGA TGAGCTGGAA
    AGGATCACAA AACTCTTCCA TTTCCTTGGG ATTTTCTTGG CCAAATGTAT TCAAGACAAT
    AGATTGGTGG ACTTGCCTAT TTCTAAGCCT TTCTTTAAAC TTATGTGCAT GGGCGACATT
    AAAAGCAATA TGAGCAAACT GATCTATGAA TCACGAGGCG ACAGAGACTT GCACTGCACT
    GAAAGTCAGT CTGAAGCGTC TACGGAAGAA GGTCATGACT CCCTCTCAGT AGGAAGCTTT
    GAAGAGGATT CAAAATCAGA ATTTATCCTT GATCCCCCTA AGCCCAAACC CCCGGCTTGG
    TTTAATGGAA TTTTAACTTG GGAAGATTTT GAACTAGTAA ATCCACACAG AGCCAGATTT
    TTAAAAGAAA TTAAAGACCT TGCTATTAAG AGGCGCCAGA TTTTAGGCAA TAAAAGCCTT
    TCCGAAGATG AGAAGAACAC AAAGTTACAG GAACTAGTAC TCAAGAACCC ATCAGGTTCT
    GGACCTCCAC TTAGCATAGA GGACTTAGGC TTAAATTTCC AGTTTTGCCC ATCTTCCAGA
    ATATATGGCT TCACAGCGGT GGACCTCAAG CCAAGTGGAG AAGATGAGAT GATAACAATG
    GATAATGCAG AAGAATATGT GGATTTGATG TTTGACTTTT GTATGCATAC GGGTATCCAG
    AAACAGATGG AAGCTTTTCG AGATGGGTTT AATAAAGTTT TTCCAATGGA GAAATTAAGT
    TCCTTCAGCC ATGAGGAAGT CCAGATGATT CTTTGTGGAA ATCAATCACC ATCCTGGGCA
    GCAGAGGACA TTATCAATTA TACTGAACCT AAGCTTGGCT ATACACGTGA CAGTCCCGGC
    TTCCTTAGGT TTGTGAGGGT CTTATGTGGC ATGTCCTCAG ACGAACGGAA AGCATTCCTG
    CAGTTTACCA CCGGCTGTTC AACTCTGCCC CCAGGTGGAC TGGCTAACCT GCATCCGAGG
    CTCACAGTCG TGCGAAAGGT TGATGCTACT GATGCAAGCT ATCCATCCGT CAACACTTGT
    GTCCACTATC TTAAGTTACC TGAATATTCT TCTGAGGAGA TCATGAGAGA GCGCTTGCTA
    GCTGCTACAA TGGAGAAAGG CTTTCATCTC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa29336
    Clone ID Related Accession (Same CDS sequence) XM_006240086.2
    Accession Version XM_006240086.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7833bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240086.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTTCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCGGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCG TCAGCAAGTA ACAGACTGTC AGTGAGCTCT
    CTGTTGGCAG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG CTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTGAG CCGGAGCAGC AGTGATAACA ACACTAACAC TCTGGGGCGG
    AATGTGATGA GCACCGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCGAC AGTGACAATG TCAACATCAA GTGTTACTAG CAGCAGCAAT
    GTAGCTACGG CGACAACAGT TTTATCAGTG GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGGCAGG AAGCAGAATA TTCCTTATAT
    GACTTTCTTG ATAGCTGTCG TGCCAGTACT CTCTTAGCTG AGCTAGATGA TGACGAGGAC
    TTGCCTGAAC CGGATGAGGA AGACGATGAG AACGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTTTGCAAC GTCGTGCTGG CTCTCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTTCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAAGAG TATGAAACTA AAGGAGGCCG CCGGAGAACC
    TGGGATGACG ACTACGTGCT GAAGCGGCAG TTCTCTGCAC TGGTTCCCGC TTTTGATCCT
    AGGCCTGGTC GTACCAATGT CCAACAGACA ACTGACCTAG AAATCCCTCC CCCAGGAACA
    CCTCACTCAG AGCTCTTGGA GGAAGTCGAG TGTACTCCAT CACCTCGATT GGCTCTCACG
    TTGAAAGTGA CTGGGCTTGG AACGACTCGG GAAGTTGAAC TGCCACTTAG CAACTTCAGA
    TCAACCATCT TTTATTATGT ACAGAAACTG CTTCAGCTGT CTTGTAATGG CAATGTGAAG
    TCAGACAAAC TTAGGCGTAT TTGGGAGCCA ACTTACACAA TCATGTATAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGGAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCTGACGCCG CTTTCCTTCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT TATAGCCGCA TATAAGGATT TTTGTGAGCA TGGAACAAGG
    TCTGGATTAA ACCAGGGGGC TAGTTCTAGT CTTCAAAGCA GTGATATTCT TAATTTGACA
    AAAGAGCAGC CTCAGGCTAA AGCCGGCAAC GGACAGAACC CCTGTGGAGT AGAGGACGTT
    CTTCAGCTTC TGCGCATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG GATATCCCAA
    GAAGATGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCTG ATGAATTTAC TAGTAAAAAG
    ATCACCACAA AAATTTTGCA GCAGATTGAG GAGCCATTGG CTTTGGCAAG CGGGGCTCTG
    CCAGACTGGT GTGAACAGCT GACCAGCAAG TGTCCTTTCC TAATCCCATT CGAAACCAGA
    CAGCTTTACT TCACCTGTAC TGCGTTTGGT GCATCCAGAG CAATAGTGTG GCTACAGAAT
    CGACGGGAAG CCACAGTGGA GCGAACAAGG ACCACGAGCA GTGTAAGGCG AGATGATCCT
    GGGGAATTTA GAGTTGGCCG ACTCAAGCAC GAAAGAGTGA AGGTTCCCCG TGGAGAGTCT
    CTGATGGAGT GGGCTGAGAA TGTCATGCAA ATACACGCAG ACCGGAAGTC AGTTCTCGAG
    GTTGAATTTC TAGGAGAAGA AGGAACTGGC CTGGGACCCA CTTTAGAGTT TTATGCTCTT
    GTGGCAGCAG AATTCCAGAG GACCGACTTG GGGACTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGACCTTGGA GGTGGGTTGA AGCCTCCTGG ATACTATGTA
    CAGCGATCAT GTGGACTGTT CACAGCGCCA TTTCCACAAG ACAGTGATGA GCTGGAAAGG
    ATCACAAAAC TCTTCCATTT CCTTGGGATT TTCTTGGCCA AATGTATTCA AGACAATAGA
    TTGGTGGACT TGCCTATTTC TAAGCCTTTC TTTAAACTTA TGTGCATGGG CGACATTAAA
    AGCAATATGA GCAAACTGAT CTATGAATCA CGAGGCGACA GAGACTTGCA CTGCACTGAA
    AGTCAGTCTG AAGCGTCTAC GGAAGAAGGT CATGACTCCC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATCCTTGAT CCCCCTAAGC CCAAACCCCC GGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGATTTTGAA CTAGTAAATC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATTAAGAGG CGCCAGATTT TAGGCAATAA AAGCCTTTCC
    GAAGATGAGA AGAACACAAA GTTACAGGAA CTAGTACTCA AGAACCCATC AGGTTCTGGA
    CCTCCACTTA GCATAGAGGA CTTAGGCTTA AATTTCCAGT TTTGCCCATC TTCCAGAATA
    TATGGCTTCA CAGCGGTGGA CCTCAAGCCA AGTGGAGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATCCAGAAA
    CAGATGGAAG CTTTTCGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AGGAAGTCCA GATGATTCTT TGTGGAAATC AATCACCATC CTGGGCAGCA
    GAGGACATTA TCAATTATAC TGAACCTAAG CTTGGCTATA CACGTGACAG TCCCGGCTTC
    CTTAGGTTTG TGAGGGTCTT ATGTGGCATG TCCTCAGACG AACGGAAAGC ATTCCTGCAG
    TTTACCACCG GCTGTTCAAC TCTGCCCCCA GGTGGACTGG CTAACCTGCA TCCGAGGCTC
    ACAGTCGTGC GAAAGGTTGA TGCTACTGAT GCAAGCTATC CATCCGTCAA CACTTGTGTC
    CACTATCTTA AGTTACCTGA ATATTCTTCT GAGGAGATCA TGAGAGAGCG CTTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006240148.1
    CDS104..7936
    Translation

    Target ORF information:

    RefSeq Version XM_006240086.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006240086.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTTCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCGGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCG TCAGCAAGTA ACAGACTGTC AGTGAGCTCT
    CTGTTGGCAG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG CTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTGAG CCGGAGCAGC AGTGATAACA ACACTAACAC TCTGGGGCGG
    AATGTGATGA GCACCGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCGAC AGTGACAATG TCAACATCAA GTGTTACTAG CAGCAGCAAT
    GTAGCTACGG CGACAACAGT TTTATCAGTG GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGGCAGG AAGCAGAATA TTCCTTATAT
    GACTTTCTTG ATAGCTGTCG TGCCAGTACT CTCTTAGCTG AGCTAGATGA TGACGAGGAC
    TTGCCTGAAC CGGATGAGGA AGACGATGAG AACGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTTTGCAAC GTCGTGCTGG CTCTCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTTCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAAGAG TATGAAACTA AAGGAGGCCG CCGGAGAACC
    TGGGATGACG ACTACGTGCT GAAGCGGCAG TTCTCTGCAC TGGTTCCCGC TTTTGATCCT
    AGGCCTGGTC GTACCAATGT CCAACAGACA ACTGACCTAG AAATCCCTCC CCCAGGAACA
    CCTCACTCAG AGCTCTTGGA GGAAGTCGAG TGTACTCCAT CACCTCGATT GGCTCTCACG
    TTGAAAGTGA CTGGGCTTGG AACGACTCGG GAAGTTGAAC TGCCACTTAG CAACTTCAGA
    TCAACCATCT TTTATTATGT ACAGAAACTG CTTCAGCTGT CTTGTAATGG CAATGTGAAG
    TCAGACAAAC TTAGGCGTAT TTGGGAGCCA ACTTACACAA TCATGTATAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGGAAA ATGGGTTGCT GGTCTATAGA GCATGTGGAA
    CAGTACCTTG GCACTGATGA ATTACCAAAG AATGACTTGA TAACCTACCT GCAGAAGAAT
    GCTGACGCCG CTTTCCTTCG CCACTGGAAA TTAACTGGCA CTAATAAAAG TATTAGGAAA
    AACAGAAATT GTTCTCAGCT TATAGCCGCA TATAAGGATT TTTGTGAGCA TGGAACAAGG
    TCTGGATTAA ACCAGGGGGC TAGTTCTAGT CTTCAAAGCA GTGATATTCT TAATTTGACA
    AAAGAGCAGC CTCAGGCTAA AGCCGGCAAC GGACAGAACC CCTGTGGAGT AGAGGACGTT
    CTTCAGCTTC TGCGCATTCT GTATATAGTT GCAAGTGACC CTTATTCAAG GATATCCCAA
    GAAGATGGTG ATGAGCAGCC CCAGTTTACT TTTCCACCTG ATGAATTTAC TAGTAAAAAG
    ATCACCACAA AAATTTTGCA GCAGATTGAG GAGCCATTGG CTTTGGCAAG CGGGGCTCTG
    CCAGACTGGT GTGAACAGCT GACCAGCAAG TGTCCTTTCC TAATCCCATT CGAAACCAGA
    CAGCTTTACT TCACCTGTAC TGCGTTTGGT GCATCCAGAG CAATAGTGTG GCTACAGAAT
    CGACGGGAAG CCACAGTGGA GCGAACAAGG ACCACGAGCA GTGTAAGGCG AGATGATCCT
    GGGGAATTTA GAGTTGGCCG ACTCAAGCAC GAAAGAGTGA AGGTTCCCCG TGGAGAGTCT
    CTGATGGAGT GGGCTGAGAA TGTCATGCAA ATACACGCAG ACCGGAAGTC AGTTCTCGAG
    GTTGAATTTC TAGGAGAAGA AGGAACTGGC CTGGGACCCA CTTTAGAGTT TTATGCTCTT
    GTGGCAGCAG AATTCCAGAG GACCGACTTG GGGACTTGGC TTTGTGATGA TAACTTTCCA
    GATGATGAAT CTCGTCATGT TGACCTTGGA GGTGGGTTGA AGCCTCCTGG ATACTATGTA
    CAGCGATCAT GTGGACTGTT CACAGCGCCA TTTCCACAAG ACAGTGATGA GCTGGAAAGG
    ATCACAAAAC TCTTCCATTT CCTTGGGATT TTCTTGGCCA AATGTATTCA AGACAATAGA
    TTGGTGGACT TGCCTATTTC TAAGCCTTTC TTTAAACTTA TGTGCATGGG CGACATTAAA
    AGCAATATGA GCAAACTGAT CTATGAATCA CGAGGCGACA GAGACTTGCA CTGCACTGAA
    AGTCAGTCTG AAGCGTCTAC GGAAGAAGGT CATGACTCCC TCTCAGTAGG AAGCTTTGAA
    GAGGATTCAA AATCAGAATT TATCCTTGAT CCCCCTAAGC CCAAACCCCC GGCTTGGTTT
    AATGGAATTT TAACTTGGGA AGATTTTGAA CTAGTAAATC CACACAGAGC CAGATTTTTA
    AAAGAAATTA AAGACCTTGC TATTAAGAGG CGCCAGATTT TAGGCAATAA AAGCCTTTCC
    GAAGATGAGA AGAACACAAA GTTACAGGAA CTAGTACTCA AGAACCCATC AGGTTCTGGA
    CCTCCACTTA GCATAGAGGA CTTAGGCTTA AATTTCCAGT TTTGCCCATC TTCCAGAATA
    TATGGCTTCA CAGCGGTGGA CCTCAAGCCA AGTGGAGAAG ATGAGATGAT AACAATGGAT
    AATGCAGAAG AATATGTGGA TTTGATGTTT GACTTTTGTA TGCATACGGG TATCCAGAAA
    CAGATGGAAG CTTTTCGAGA TGGGTTTAAT AAAGTTTTTC CAATGGAGAA ATTAAGTTCC
    TTCAGCCATG AGGAAGTCCA GATGATTCTT TGTGGAAATC AATCACCATC CTGGGCAGCA
    GAGGACATTA TCAATTATAC TGAACCTAAG CTTGGCTATA CACGTGACAG TCCCGGCTTC
    CTTAGGTTTG TGAGGGTCTT ATGTGGCATG TCCTCAGACG AACGGAAAGC ATTCCTGCAG
    TTTACCACCG GCTGTTCAAC TCTGCCCCCA GGTGGACTGG CTAACCTGCA TCCGAGGCTC
    ACAGTCGTGC GAAAGGTTGA TGCTACTGAT GCAAGCTATC CATCCGTCAA CACTTGTGTC
    CACTATCTTA AGTTACCTGA ATATTCTTCT GAGGAGATCA TGAGAGAGCG CTTGCTAGCT
    GCTACAATGG AGAAAGGCTT TCATCTCAAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa29337
    Clone ID Related Accession (Same CDS sequence) XM_006240087.2
    Accession Version XM_006240087.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240087.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTTCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCGGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCG TCAGCAAGTA ACAGACTGTC AGTGAGCTCT
    CTGTTGGCAG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG CTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTGAG CCGGAGCAGC AGTGATAACA ACACTAACAC TCTGGGGCGG
    AATGTGATGA GCACCGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCGAC AGTGACAATG TCAACATCAA GTGTTACTAG CAGCAGCAAT
    GTAGCTACGG CGACAACAGT TTTATCAGTG GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGGCAGG AAGCAGAATA TTCCTTATAT
    GACTTTCTTG ATAGCTGTCG TGCCAGTACT CTCTTAGCTG AGCTAGATGA TGACGAGGAC
    TTGCCTGAAC CGGATGAGGA AGACGATGAG AACGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCATCTTTG CAACGTCGTG CTGGCTCTCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCTT CTGGAGCAGG GAGCCGACCT
    ATTGGGGAAC AGGAAGAAGA AGAGTATGAA ACTAAAGGAG GCCGCCGGAG AACCTGGGAT
    GACGACTACG TGCTGAAGCG GCAGTTCTCT GCACTGGTTC CCGCTTTTGA TCCTAGGCCT
    GGTCGTACCA ATGTCCAACA GACAACTGAC CTAGAAATCC CTCCCCCAGG AACACCTCAC
    TCAGAGCTCT TGGAGGAAGT CGAGTGTACT CCATCACCTC GATTGGCTCT CACGTTGAAA
    GTGACTGGGC TTGGAACGAC TCGGGAAGTT GAACTGCCAC TTAGCAACTT CAGATCAACC
    ATCTTTTATT ATGTACAGAA ACTGCTTCAG CTGTCTTGTA ATGGCAATGT GAAGTCAGAC
    AAACTTAGGC GTATTTGGGA GCCAACTTAC ACAATCATGT ATAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG GAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCTGAC
    GCCGCTTTCC TTCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTTATAGC CGCATATAAG GATTTTTGTG AGCATGGAAC AAGGTCTGGA
    TTAAACCAGG GGGCTAGTTC TAGTCTTCAA AGCAGTGATA TTCTTAATTT GACAAAAGAG
    CAGCCTCAGG CTAAAGCCGG CAACGGACAG AACCCCTGTG GAGTAGAGGA CGTTCTTCAG
    CTTCTGCGCA TTCTGTATAT AGTTGCAAGT GACCCTTATT CAAGGATATC CCAAGAAGAT
    GGTGATGAGC AGCCCCAGTT TACTTTTCCA CCTGATGAAT TTACTAGTAA AAAGATCACC
    ACAAAAATTT TGCAGCAGAT TGAGGAGCCA TTGGCTTTGG CAAGCGGGGC TCTGCCAGAC
    TGGTGTGAAC AGCTGACCAG CAAGTGTCCT TTCCTAATCC CATTCGAAAC CAGACAGCTT
    TACTTCACCT GTACTGCGTT TGGTGCATCC AGAGCAATAG TGTGGCTACA GAATCGACGG
    GAAGCCACAG TGGAGCGAAC AAGGACCACG AGCAGTGTAA GGCGAGATGA TCCTGGGGAA
    TTTAGAGTTG GCCGACTCAA GCACGAAAGA GTGAAGGTTC CCCGTGGAGA GTCTCTGATG
    GAGTGGGCTG AGAATGTCAT GCAAATACAC GCAGACCGGA AGTCAGTTCT CGAGGTTGAA
    TTTCTAGGAG AAGAAGGAAC TGGCCTGGGA CCCACTTTAG AGTTTTATGC TCTTGTGGCA
    GCAGAATTCC AGAGGACCGA CTTGGGGACT TGGCTTTGTG ATGATAACTT TCCAGATGAT
    GAATCTCGTC ATGTTGACCT TGGAGGTGGG TTGAAGCCTC CTGGATACTA TGTACAGCGA
    TCATGTGGAC TGTTCACAGC GCCATTTCCA CAAGACAGTG ATGAGCTGGA AAGGATCACA
    AAACTCTTCC ATTTCCTTGG GATTTTCTTG GCCAAATGTA TTCAAGACAA TAGATTGGTG
    GACTTGCCTA TTTCTAAGCC TTTCTTTAAA CTTATGTGCA TGGGCGACAT TAAAAGCAAT
    ATGAGCAAAC TGATCTATGA ATCACGAGGC GACAGAGACT TGCACTGCAC TGAAAGTCAG
    TCTGAAGCGT CTACGGAAGA AGGTCATGAC TCCCTCTCAG TAGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATCCT TGATCCCCCT AAGCCCAAAC CCCCGGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGATTT TGAACTAGTA AATCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATTAA GAGGCGCCAG ATTTTAGGCA ATAAAAGCCT TTCCGAAGAT
    GAGAAGAACA CAAAGTTACA GGAACTAGTA CTCAAGAACC CATCAGGTTC TGGACCTCCA
    CTTAGCATAG AGGACTTAGG CTTAAATTTC CAGTTTTGCC CATCTTCCAG AATATATGGC
    TTCACAGCGG TGGACCTCAA GCCAAGTGGA GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATCCA GAAACAGATG
    GAAGCTTTTC GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAGGAAG TCCAGATGAT TCTTTGTGGA AATCAATCAC CATCCTGGGC AGCAGAGGAC
    ATTATCAATT ATACTGAACC TAAGCTTGGC TATACACGTG ACAGTCCCGG CTTCCTTAGG
    TTTGTGAGGG TCTTATGTGG CATGTCCTCA GACGAACGGA AAGCATTCCT GCAGTTTACC
    ACCGGCTGTT CAACTCTGCC CCCAGGTGGA CTGGCTAACC TGCATCCGAG GCTCACAGTC
    GTGCGAAAGG TTGATGCTAC TGATGCAAGC TATCCATCCG TCAACACTTG TGTCCACTAT
    CTTAAGTTAC CTGAATATTC TTCTGAGGAG ATCATGAGAG AGCGCTTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006240149.1
    CDS104..7930
    Translation

    Target ORF information:

    RefSeq Version XM_006240087.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006240087.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTTCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCGGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCG TCAGCAAGTA ACAGACTGTC AGTGAGCTCT
    CTGTTGGCAG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG CTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTGAG CCGGAGCAGC AGTGATAACA ACACTAACAC TCTGGGGCGG
    AATGTGATGA GCACCGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCGAC AGTGACAATG TCAACATCAA GTGTTACTAG CAGCAGCAAT
    GTAGCTACGG CGACAACAGT TTTATCAGTG GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGGCAGG AAGCAGAATA TTCCTTATAT
    GACTTTCTTG ATAGCTGTCG TGCCAGTACT CTCTTAGCTG AGCTAGATGA TGACGAGGAC
    TTGCCTGAAC CGGATGAGGA AGACGATGAG AACGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGA TTCTGAGACG TCCATCTTTG CAACGTCGTG CTGGCTCTCG CTCTGATGTA
    ACACACCATG CTGTCACCTC ACAGCTACCA CAGGTCCCTT CTGGAGCAGG GAGCCGACCT
    ATTGGGGAAC AGGAAGAAGA AGAGTATGAA ACTAAAGGAG GCCGCCGGAG AACCTGGGAT
    GACGACTACG TGCTGAAGCG GCAGTTCTCT GCACTGGTTC CCGCTTTTGA TCCTAGGCCT
    GGTCGTACCA ATGTCCAACA GACAACTGAC CTAGAAATCC CTCCCCCAGG AACACCTCAC
    TCAGAGCTCT TGGAGGAAGT CGAGTGTACT CCATCACCTC GATTGGCTCT CACGTTGAAA
    GTGACTGGGC TTGGAACGAC TCGGGAAGTT GAACTGCCAC TTAGCAACTT CAGATCAACC
    ATCTTTTATT ATGTACAGAA ACTGCTTCAG CTGTCTTGTA ATGGCAATGT GAAGTCAGAC
    AAACTTAGGC GTATTTGGGA GCCAACTTAC ACAATCATGT ATAGAGAAAT GAAGGATTCT
    GATAAAGAAA AGGAAAATGG GAAAATGGGT TGCTGGTCTA TAGAGCATGT GGAACAGTAC
    CTTGGCACTG ATGAATTACC AAAGAATGAC TTGATAACCT ACCTGCAGAA GAATGCTGAC
    GCCGCTTTCC TTCGCCACTG GAAATTAACT GGCACTAATA AAAGTATTAG GAAAAACAGA
    AATTGTTCTC AGCTTATAGC CGCATATAAG GATTTTTGTG AGCATGGAAC AAGGTCTGGA
    TTAAACCAGG GGGCTAGTTC TAGTCTTCAA AGCAGTGATA TTCTTAATTT GACAAAAGAG
    CAGCCTCAGG CTAAAGCCGG CAACGGACAG AACCCCTGTG GAGTAGAGGA CGTTCTTCAG
    CTTCTGCGCA TTCTGTATAT AGTTGCAAGT GACCCTTATT CAAGGATATC CCAAGAAGAT
    GGTGATGAGC AGCCCCAGTT TACTTTTCCA CCTGATGAAT TTACTAGTAA AAAGATCACC
    ACAAAAATTT TGCAGCAGAT TGAGGAGCCA TTGGCTTTGG CAAGCGGGGC TCTGCCAGAC
    TGGTGTGAAC AGCTGACCAG CAAGTGTCCT TTCCTAATCC CATTCGAAAC CAGACAGCTT
    TACTTCACCT GTACTGCGTT TGGTGCATCC AGAGCAATAG TGTGGCTACA GAATCGACGG
    GAAGCCACAG TGGAGCGAAC AAGGACCACG AGCAGTGTAA GGCGAGATGA TCCTGGGGAA
    TTTAGAGTTG GCCGACTCAA GCACGAAAGA GTGAAGGTTC CCCGTGGAGA GTCTCTGATG
    GAGTGGGCTG AGAATGTCAT GCAAATACAC GCAGACCGGA AGTCAGTTCT CGAGGTTGAA
    TTTCTAGGAG AAGAAGGAAC TGGCCTGGGA CCCACTTTAG AGTTTTATGC TCTTGTGGCA
    GCAGAATTCC AGAGGACCGA CTTGGGGACT TGGCTTTGTG ATGATAACTT TCCAGATGAT
    GAATCTCGTC ATGTTGACCT TGGAGGTGGG TTGAAGCCTC CTGGATACTA TGTACAGCGA
    TCATGTGGAC TGTTCACAGC GCCATTTCCA CAAGACAGTG ATGAGCTGGA AAGGATCACA
    AAACTCTTCC ATTTCCTTGG GATTTTCTTG GCCAAATGTA TTCAAGACAA TAGATTGGTG
    GACTTGCCTA TTTCTAAGCC TTTCTTTAAA CTTATGTGCA TGGGCGACAT TAAAAGCAAT
    ATGAGCAAAC TGATCTATGA ATCACGAGGC GACAGAGACT TGCACTGCAC TGAAAGTCAG
    TCTGAAGCGT CTACGGAAGA AGGTCATGAC TCCCTCTCAG TAGGAAGCTT TGAAGAGGAT
    TCAAAATCAG AATTTATCCT TGATCCCCCT AAGCCCAAAC CCCCGGCTTG GTTTAATGGA
    ATTTTAACTT GGGAAGATTT TGAACTAGTA AATCCACACA GAGCCAGATT TTTAAAAGAA
    ATTAAAGACC TTGCTATTAA GAGGCGCCAG ATTTTAGGCA ATAAAAGCCT TTCCGAAGAT
    GAGAAGAACA CAAAGTTACA GGAACTAGTA CTCAAGAACC CATCAGGTTC TGGACCTCCA
    CTTAGCATAG AGGACTTAGG CTTAAATTTC CAGTTTTGCC CATCTTCCAG AATATATGGC
    TTCACAGCGG TGGACCTCAA GCCAAGTGGA GAAGATGAGA TGATAACAAT GGATAATGCA
    GAAGAATATG TGGATTTGAT GTTTGACTTT TGTATGCATA CGGGTATCCA GAAACAGATG
    GAAGCTTTTC GAGATGGGTT TAATAAAGTT TTTCCAATGG AGAAATTAAG TTCCTTCAGC
    CATGAGGAAG TCCAGATGAT TCTTTGTGGA AATCAATCAC CATCCTGGGC AGCAGAGGAC
    ATTATCAATT ATACTGAACC TAAGCTTGGC TATACACGTG ACAGTCCCGG CTTCCTTAGG
    TTTGTGAGGG TCTTATGTGG CATGTCCTCA GACGAACGGA AAGCATTCCT GCAGTTTACC
    ACCGGCTGTT CAACTCTGCC CCCAGGTGGA CTGGCTAACC TGCATCCGAG GCTCACAGTC
    GTGCGAAAGG TTGATGCTAC TGATGCAAGC TATCCATCCG TCAACACTTG TGTCCACTAT
    CTTAAGTTAC CTGAATATTC TTCTGAGGAG ATCATGAGAG AGCGCTTGCT AGCTGCTACA
    ATGGAGAAAG GCTTTCATCT CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa29338
    Clone ID Related Accession (Same CDS sequence) XM_006240088.2
    Accession Version XM_006240088.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006240088.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAGTCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTTCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCGGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCGT CAGCAAGTAA CAGACTGTCA GTGAGCTCTC TGTTGGCAGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGC
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGGAGCAGCA GTGATAACAA CACTAACACT CTGGGGCGGA ATGTGATGAG CACCGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCGACA
    GTGACAATGT CAACATCAAG TGTTACTAGC AGCAGCAATG TAGCTACGGC GACAACAGTT
    TTATCAGTGG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGGCAGGA AGCAGAATAT TCCTTATATG ACTTTCTTGA TAGCTGTCGT
    GCCAGTACTC TCTTAGCTGA GCTAGATGAT GACGAGGACT TGCCTGAACC GGATGAGGAA
    GACGATGAGA ACGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCAT CTTTGCAACG TCGTGCTGGC TCTCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCTTCTGGA GCAGGGAGCC GACCTATTGG GGAACAGGAA
    GAAGAAGAGT ATGAAACTAA AGGAGGCCGC CGGAGAACCT GGGATGACGA CTACGTGCTG
    AAGCGGCAGT TCTCTGCACT GGTTCCCGCT TTTGATCCTA GGCCTGGTCG TACCAATGTC
    CAACAGACAA CTGACCTAGA AATCCCTCCC CCAGGAACAC CTCACTCAGA GCTCTTGGAG
    GAAGTCGAGT GTACTCCATC ACCTCGATTG GCTCTCACGT TGAAAGTGAC TGGGCTTGGA
    ACGACTCGGG AAGTTGAACT GCCACTTAGC AACTTCAGAT CAACCATCTT TTATTATGTA
    CAGAAACTGC TTCAGCTGTC TTGTAATGGC AATGTGAAGT CAGACAAACT TAGGCGTATT
    TGGGAGCCAA CTTACACAAT CATGTATAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGGAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CTGACGCCGC TTTCCTTCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTT
    ATAGCCGCAT ATAAGGATTT TTGTGAGCAT GGAACAAGGT CTGGATTAAA CCAGGGGGCT
    AGTTCTAGTC TTCAAAGCAG TGATATTCTT AATTTGACAA AAGAGCAGCC TCAGGCTAAA
    GCCGGCAACG GACAGAACCC CTGTGGAGTA GAGGACGTTC TTCAGCTTCT GCGCATTCTG
    TATATAGTTG CAAGTGACCC TTATTCAAGG ATATCCCAAG AAGATGGTGA TGAGCAGCCC
    CAGTTTACTT TTCCACCTGA TGAATTTACT AGTAAAAAGA TCACCACAAA AATTTTGCAG
    CAGATTGAGG AGCCATTGGC TTTGGCAAGC GGGGCTCTGC CAGACTGGTG TGAACAGCTG
    ACCAGCAAGT GTCCTTTCCT AATCCCATTC GAAACCAGAC AGCTTTACTT CACCTGTACT
    GCGTTTGGTG CATCCAGAGC AATAGTGTGG CTACAGAATC GACGGGAAGC CACAGTGGAG
    CGAACAAGGA CCACGAGCAG TGTAAGGCGA GATGATCCTG GGGAATTTAG AGTTGGCCGA
    CTCAAGCACG AAAGAGTGAA GGTTCCCCGT GGAGAGTCTC TGATGGAGTG GGCTGAGAAT
    GTCATGCAAA TACACGCAGA CCGGAAGTCA GTTCTCGAGG TTGAATTTCT AGGAGAAGAA
    GGAACTGGCC TGGGACCCAC TTTAGAGTTT TATGCTCTTG TGGCAGCAGA ATTCCAGAGG
    ACCGACTTGG GGACTTGGCT TTGTGATGAT AACTTTCCAG ATGATGAATC TCGTCATGTT
    GACCTTGGAG GTGGGTTGAA GCCTCCTGGA TACTATGTAC AGCGATCATG TGGACTGTTC
    ACAGCGCCAT TTCCACAAGA CAGTGATGAG CTGGAAAGGA TCACAAAACT CTTCCATTTC
    CTTGGGATTT TCTTGGCCAA ATGTATTCAA GACAATAGAT TGGTGGACTT GCCTATTTCT
    AAGCCTTTCT TTAAACTTAT GTGCATGGGC GACATTAAAA GCAATATGAG CAAACTGATC
    TATGAATCAC GAGGCGACAG AGACTTGCAC TGCACTGAAA GTCAGTCTGA AGCGTCTACG
    GAAGAAGGTC ATGACTCCCT CTCAGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATCCTTGATC CCCCTAAGCC CAAACCCCCG GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GATTTTGAAC TAGTAAATCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATTAAGAGGC GCCAGATTTT AGGCAATAAA AGCCTTTCCG AAGATGAGAA GAACACAAAG
    TTACAGGAAC TAGTACTCAA GAACCCATCA GGTTCTGGAC CTCCACTTAG CATAGAGGAC
    TTAGGCTTAA ATTTCCAGTT TTGCCCATCT TCCAGAATAT ATGGCTTCAC AGCGGTGGAC
    CTCAAGCCAA GTGGAGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGTAT GCATACGGGT ATCCAGAAAC AGATGGAAGC TTTTCGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA GGAAGTCCAG
    ATGATTCTTT GTGGAAATCA ATCACCATCC TGGGCAGCAG AGGACATTAT CAATTATACT
    GAACCTAAGC TTGGCTATAC ACGTGACAGT CCCGGCTTCC TTAGGTTTGT GAGGGTCTTA
    TGTGGCATGT CCTCAGACGA ACGGAAAGCA TTCCTGCAGT TTACCACCGG CTGTTCAACT
    CTGCCCCCAG GTGGACTGGC TAACCTGCAT CCGAGGCTCA CAGTCGTGCG AAAGGTTGAT
    GCTACTGATG CAAGCTATCC ATCCGTCAAC ACTTGTGTCC ACTATCTTAA GTTACCTGAA
    TATTCTTCTG AGGAGATCAT GAGAGAGCGC TTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006240150.1
    CDS104..7795
    Translation

    Target ORF information:

    RefSeq Version XM_006240088.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006240088.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCACAACG CAGTCATGGA GCAGCTTGGT GAAAAACAAC
    TGTCCAGACA AGACGTCTGC TGCTGCAGGC TCCTCAAGTA GAAAAGGAAG CAGCAGTTCT
    GTGTGTAGCG TGGCCAGTAG CAGCGACATC AGCTTGGGTT CGACCAAAAC GGAACGGAGA
    TCAGAAATTG TAATGGAACA CAGTATAGTT TCAGGAGCTG ATGTCCATGA ACCAATTGTT
    GTTCTTTCAT CTGCTGAAAA CGTTCCTCAA ACAGAAGTAG GGTCATCTTC CAGTGCAAGC
    ACCAGCACCC TAACAGCGGA AACGGGAAGT GAAAATGCTG AAAGGAAGTT AGGCCCGGAT
    AGTTCTGTTC GAGCTCCTGG GGAGTCTAGT GCAATATCCA TGGGAATTGT TAGCGTTAGC
    TCTCCTGATG TTAGTTCAGT ATCTGAATTA ACTAATAAGG AAGCAGCTTC ACAACGACCC
    CTTAGCTCGT CAGCAAGTAA CAGACTGTCA GTGAGCTCTC TGTTGGCAGC TGGGGCCCCT
    ATGAGCTCTA GTGCAAGTGT ACCTAACCTG TCCTCAAGAG AAACATCTAG CTTGGAGAGC
    TTTGTAAGGA GAGTGGCAAA CATAGCACGG ACTAATGCCA CCAACAACAT GAACCTGAGC
    CGGAGCAGCA GTGATAACAA CACTAACACT CTGGGGCGGA ATGTGATGAG CACCGCAACT
    TCTCCTCTTA TGGGTGCTCA GAGTTTCCCT AATTTGACCA CACCTGGTAC TACATCGACA
    GTGACAATGT CAACATCAAG TGTTACTAGC AGCAGCAATG TAGCTACGGC GACAACAGTT
    TTATCAGTGG GTCAGTCATT AAGTAATACT TTAACCACCA GCCTCACATC AACCTCTAGT
    GAGAGTGACA CAGGGCAGGA AGCAGAATAT TCCTTATATG ACTTTCTTGA TAGCTGTCGT
    GCCAGTACTC TCTTAGCTGA GCTAGATGAT GACGAGGACT TGCCTGAACC GGATGAGGAA
    GACGATGAGA ACGAAGATGA CAATCAGGAG GACCAAGAAT ACGAGGAGGT TATGATTCTG
    AGACGTCCAT CTTTGCAACG TCGTGCTGGC TCTCGCTCTG ATGTAACACA CCATGCTGTC
    ACCTCACAGC TACCACAGGT CCCTTCTGGA GCAGGGAGCC GACCTATTGG GGAACAGGAA
    GAAGAAGAGT ATGAAACTAA AGGAGGCCGC CGGAGAACCT GGGATGACGA CTACGTGCTG
    AAGCGGCAGT TCTCTGCACT GGTTCCCGCT TTTGATCCTA GGCCTGGTCG TACCAATGTC
    CAACAGACAA CTGACCTAGA AATCCCTCCC CCAGGAACAC CTCACTCAGA GCTCTTGGAG
    GAAGTCGAGT GTACTCCATC ACCTCGATTG GCTCTCACGT TGAAAGTGAC TGGGCTTGGA
    ACGACTCGGG AAGTTGAACT GCCACTTAGC AACTTCAGAT CAACCATCTT TTATTATGTA
    CAGAAACTGC TTCAGCTGTC TTGTAATGGC AATGTGAAGT CAGACAAACT TAGGCGTATT
    TGGGAGCCAA CTTACACAAT CATGTATAGA GAAATGAAGG ATTCTGATAA AGAAAAGGAA
    AATGGGAAAA TGGGTTGCTG GTCTATAGAG CATGTGGAAC AGTACCTTGG CACTGATGAA
    TTACCAAAGA ATGACTTGAT AACCTACCTG CAGAAGAATG CTGACGCCGC TTTCCTTCGC
    CACTGGAAAT TAACTGGCAC TAATAAAAGT ATTAGGAAAA ACAGAAATTG TTCTCAGCTT
    ATAGCCGCAT ATAAGGATTT TTGTGAGCAT GGAACAAGGT CTGGATTAAA CCAGGGGGCT
    AGTTCTAGTC TTCAAAGCAG TGATATTCTT AATTTGACAA AAGAGCAGCC TCAGGCTAAA
    GCCGGCAACG GACAGAACCC CTGTGGAGTA GAGGACGTTC TTCAGCTTCT GCGCATTCTG
    TATATAGTTG CAAGTGACCC TTATTCAAGG ATATCCCAAG AAGATGGTGA TGAGCAGCCC
    CAGTTTACTT TTCCACCTGA TGAATTTACT AGTAAAAAGA TCACCACAAA AATTTTGCAG
    CAGATTGAGG AGCCATTGGC TTTGGCAAGC GGGGCTCTGC CAGACTGGTG TGAACAGCTG
    ACCAGCAAGT GTCCTTTCCT AATCCCATTC GAAACCAGAC AGCTTTACTT CACCTGTACT
    GCGTTTGGTG CATCCAGAGC AATAGTGTGG CTACAGAATC GACGGGAAGC CACAGTGGAG
    CGAACAAGGA CCACGAGCAG TGTAAGGCGA GATGATCCTG GGGAATTTAG AGTTGGCCGA
    CTCAAGCACG AAAGAGTGAA GGTTCCCCGT GGAGAGTCTC TGATGGAGTG GGCTGAGAAT
    GTCATGCAAA TACACGCAGA CCGGAAGTCA GTTCTCGAGG TTGAATTTCT AGGAGAAGAA
    GGAACTGGCC TGGGACCCAC TTTAGAGTTT TATGCTCTTG TGGCAGCAGA ATTCCAGAGG
    ACCGACTTGG GGACTTGGCT TTGTGATGAT AACTTTCCAG ATGATGAATC TCGTCATGTT
    GACCTTGGAG GTGGGTTGAA GCCTCCTGGA TACTATGTAC AGCGATCATG TGGACTGTTC
    ACAGCGCCAT TTCCACAAGA CAGTGATGAG CTGGAAAGGA TCACAAAACT CTTCCATTTC
    CTTGGGATTT TCTTGGCCAA ATGTATTCAA GACAATAGAT TGGTGGACTT GCCTATTTCT
    AAGCCTTTCT TTAAACTTAT GTGCATGGGC GACATTAAAA GCAATATGAG CAAACTGATC
    TATGAATCAC GAGGCGACAG AGACTTGCAC TGCACTGAAA GTCAGTCTGA AGCGTCTACG
    GAAGAAGGTC ATGACTCCCT CTCAGTAGGA AGCTTTGAAG AGGATTCAAA ATCAGAATTT
    ATCCTTGATC CCCCTAAGCC CAAACCCCCG GCTTGGTTTA ATGGAATTTT AACTTGGGAA
    GATTTTGAAC TAGTAAATCC ACACAGAGCC AGATTTTTAA AAGAAATTAA AGACCTTGCT
    ATTAAGAGGC GCCAGATTTT AGGCAATAAA AGCCTTTCCG AAGATGAGAA GAACACAAAG
    TTACAGGAAC TAGTACTCAA GAACCCATCA GGTTCTGGAC CTCCACTTAG CATAGAGGAC
    TTAGGCTTAA ATTTCCAGTT TTGCCCATCT TCCAGAATAT ATGGCTTCAC AGCGGTGGAC
    CTCAAGCCAA GTGGAGAAGA TGAGATGATA ACAATGGATA ATGCAGAAGA ATATGTGGAT
    TTGATGTTTG ACTTTTGTAT GCATACGGGT ATCCAGAAAC AGATGGAAGC TTTTCGAGAT
    GGGTTTAATA AAGTTTTTCC AATGGAGAAA TTAAGTTCCT TCAGCCATGA GGAAGTCCAG
    ATGATTCTTT GTGGAAATCA ATCACCATCC TGGGCAGCAG AGGACATTAT CAATTATACT
    GAACCTAAGC TTGGCTATAC ACGTGACAGT CCCGGCTTCC TTAGGTTTGT GAGGGTCTTA
    TGTGGCATGT CCTCAGACGA ACGGAAAGCA TTCCTGCAGT TTACCACCGG CTGTTCAACT
    CTGCCCCCAG GTGGACTGGC TAACCTGCAT CCGAGGCTCA CAGTCGTGCG AAAGGTTGAT
    GCTACTGATG CAAGCTATCC ATCCGTCAAC ACTTGTGTCC ACTATCTTAA GTTACCTGAA
    TATTCTTCTG AGGAGATCAT GAGAGAGCGC TTGCTAGCTG CTACAATGGA GAAAGGCTTT
    CATCTCAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa29339
    Clone ID Related Accession (Same CDS sequence) XM_017594442.1
    Accession Version XM_017594442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECTD1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTTCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCGGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCG TCAGCAAGTA ACAGACTGTC AGTGAGCTCT
    CTGTTGGCAG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG CTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTGAG CCGGAGCAGC AGTGATAACA ACACTAACAC TCTGGGGCGG
    AATGTGATGA GCACCGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCGAC AGTGACAATG TCAACATCAA GTGTTACTAG CAGCAGCAAT
    GTAGCTACGG CGACAACAGT TTTATCAGTG GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGGCAGG AAGCAGAATA TTCCTTATAT
    GACTTTCTTG ATAGCTGTCG TGCCAGTACT CTCTTAGCTG AGCTAGATGA TGACGAGGAC
    TTGCCTGAAC CGGATGAGGA AGACGATGAG AACGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTTTGCAAC GTCGTGCTGG CTCTCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTTCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAAGAG TATGAAACTA AAGGAGGCCG CCGGAGAACC
    TGGGATGACG ACTACGTGCT GAAGCGGCAG TTCTCTGCAC TGGTTCCCGC TTTTGATCCT
    AGGCCTGGTC GTACCAATGT CCAACAGACA ACTGACCTAG AAATCCCTCC CCCAGGAACA
    CCTCACTCAG AGCTCTTGGA GGAAGTCGAG TGTACTCCAT CACCTCGATT GGCTCTCACG
    TTGAAAGTGA CTGGGCTTGG AACGACTCGG GAAGTTGAAC TGCCACTTAG CAACTTCAGA
    TCAACCATCT TTTATTATGT ACAGAAACTG CTTCAGCTGT CTTGTAATGG CAATGTGAAG
    TCAGACAAAC TTAGGCGTAT TTGGGAGCCA ACTTACACAA TCATGTATAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGGAAA ATGGATTTTT GTGAGCATGG AACAAGGTCT
    GGATTAAACC AGGGGGCTAG TTCTAGTCTT CAAAGCAGTG ATATTCTTAA TTTGACAAAA
    GAGCAGCCTC AGGCTAAAGC CGGCAACGGA CAGAACCCCT GTGGAGTAGA GGACGTTCTT
    CAGCTTCTGC GCATTCTGTA TATAGTTGCA AGTGACCCTT ATTCAAGGAT ATCCCAAGAA
    GATGGTGATG AGCAGCCCCA GTTTACTTTT CCACCTGATG AATTTACTAG TAAAAAGATC
    ACCACAAAAA TTTTGCAGCA GATTGAGGAG CCATTGGCTT TGGCAAGCGG GGCTCTGCCA
    GACTGGTGTG AACAGCTGAC CAGCAAGTGT CCTTTCCTAA TCCCATTCGA AACCAGACAG
    CTTTACTTCA CCTGTACTGC GTTTGGTGCA TCCAGAGCAA TAGTGTGGCT ACAGAATCGA
    CGGGAAGCCA CAGTGGAGCG AACAAGGACC ACGAGCAGTG TAAGGCGAGA TGATCCTGGG
    GAATTTAGAG TTGGCCGACT CAAGCACGAA AGAGTGAAGG TTCCCCGTGG AGAGTCTCTG
    ATGGAGTGGG CTGAGAATGT CATGCAAATA CACGCAGACC GGAAGTCAGT TCTCGAGGTT
    GAATTTCTAG GAGAAGAAGG AACTGGCCTG GGACCCACTT TAGAGTTTTA TGCTCTTGTG
    GCAGCAGAAT TCCAGAGGAC CGACTTGGGG ACTTGGCTTT GTGATGATAA CTTTCCAGAT
    GATGAATCTC GTCATGTTGA CCTTGGAGGT GGGTTGAAGC CTCCTGGATA CTATGTACAG
    CGATCATGTG GACTGTTCAC AGCGCCATTT CCACAAGACA GTGATGAGCT GGAAAGGATC
    ACAAAACTCT TCCATTTCCT TGGGATTTTC TTGGCCAAAT GTATTCAAGA CAATAGATTG
    GTGGACTTGC CTATTTCTAA GCCTTTCTTT AAACTTATGT GCATGGGCGA CATTAAAAGC
    AATATGAGCA AACTGATCTA TGAATCACGA GGCGACAGAG ACTTGCACTG CACTGAAAGT
    CAGTCTGAAG CGTCTACGGA AGAAGGTCAT GACTCCCTCT CAGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT CCTTGATCCC CCTAAGCCCA AACCCCCGGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA TTTTGAACTA GTAAATCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT TAAGAGGCGC CAGATTTTAG GCAATAAAAG CCTTTCCGAA
    GATGAGAAGA ACACAAAGTT ACAGGAACTA GTACTCAAGA ACCCATCAGG TTCTGGACCT
    CCACTTAGCA TAGAGGACTT AGGCTTAAAT TTCCAGTTTT GCCCATCTTC CAGAATATAT
    GGCTTCACAG CGGTGGACCT CAAGCCAAGT GGAGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT CCAGAAACAG
    ATGGAAGCTT TTCGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAGG AAGTCCAGAT GATTCTTTGT GGAAATCAAT CACCATCCTG GGCAGCAGAG
    GACATTATCA ATTATACTGA ACCTAAGCTT GGCTATACAC GTGACAGTCC CGGCTTCCTT
    AGGTTTGTGA GGGTCTTATG TGGCATGTCC TCAGACGAAC GGAAAGCATT CCTGCAGTTT
    ACCACCGGCT GTTCAACTCT GCCCCCAGGT GGACTGGCTA ACCTGCATCC GAGGCTCACA
    GTCGTGCGAA AGGTTGATGC TACTGATGCA AGCTATCCAT CCGTCAACAC TTGTGTCCAC
    TATCTTAAGT TACCTGAATA TTCTTCTGAG GAGATCATGA GAGAGCGCTT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017449931.1
    CDS104..7753
    Translation

    Target ORF information:

    RefSeq Version XM_017594442.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT domain E3 ubiquitin protein ligase 1 (Hectd1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017594442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    ATGGCAGATG TGGATCCTGA TACGTTGCTG GAGTGGCTGC AGATGGGACA GGGAGATGAA 
    AGGGACATGC AGCTAATAGC CCTGGAACAG CTATGCATGT TGCTTTTGAT GTCTGACAAT
    GTGGATCGGT GTTTTGAAAC ATGTCCACCT CGTACTTTCC TACCAGCTCT CTGCAAAATT
    TTTCTTGATG AAAGTGCTCC AGACAATGTC TTAGAGGTGA CAGCTCGTGC CATAACGTAC
    TACCTGGATG TGTCTGCAGA GTGCACCCGA AGGATTGTAG GGGTGGATGG GGCTATAAAA
    GCACTCTGTA ATCGCTTGGT GGTGGTTGAA CTCAACAACA GAACCAGCAG AGACTTAGCT
    GAACAGTGTG TAAAGGTGCT GGAACTGATA TGCACTCGTG AGTCAGGAGC AGTCTTTGAG
    GCTGGTGGTT TGAATTGTGT TCTCACCTTC ATTCGTGACA GCGGACACCT AGTTCATAAA
    GATACATTAC ACTCTGCGAT GGCGGTGGTA TCGAGACTCT GTGGTAAAAT GGAACCTCAG
    GATTCTTCTC TAGAAATTTG TGTAGAGTCT CTATCTAGTT TATTGAAACA TGAAGATCAT
    CAGGTTTCAG ACGGAGCTCT GAGGTGTTTT GCATCACTAG CCGACCGATT TACTCGTCGA
    GGAGTGGACC CAGCTCCATT GGCCAAGCAC GGCTTAACTG AGGAGCTGTT ATCTCGAATG
    GCAGCTGCTG GTGGCACTGT ATCAGGACCG TCTTCAGCAT GCAAACCGGG TCGCAGTACC
    ACAGGAGCCC CCTCTGCAGC TGCAGATTCC AAATTGAGTA ACCAGGTGTC AACAATTGTA
    AGTCTTCTCT CCACACTTTG CAGAGGCTCT CCACTAGTAA CACACGATCT TCTGAGGTCA
    GAGCTTCCAG ATTCAATTGA AAGTGCATTA CAGGGTGATG AAAGATGTGT GCTTGATACC
    ATGCGTCTGG TTGACCTCCT CTTGGTGCTG TTATTTGAAG GACGAAAGGC TTTGCCTAAG
    TCTAGTGCTG GATCTACAGG CAGAATCCCA GGACTACGGA GACTGGATAG TTCTGGGGAA
    CGCTCCCATC GGCAGCTTAT AGACTGTATT CGAAGTAAAG ATACTGATGC TCTCATAGAT
    GCAATTGACA CAGGAGCCTT TGAAGTAAAT TTTATGGATG ATGTGGGTCA GACTCTATTA
    AATTGGGCCT CTGCGTTTGG AACTCAGGAA ATGGTAGAAT TTCTGTGTGA GAGGGGCGCT
    GACGTTAATA GAGGCCAAAG GTCCTCATCG TTACACTATG CTGCATGTTT TGGAAGACCT
    CAAGTAGCAA AGACTCTGTT ACGGCATGGT GCAAATCCGG ATCTCAGAGA TGAAGATGGG
    AAAACACCAT TGGATAAAGC TCGAGAAAGA GGCCACAGTG AAGTAGTAGC AATTCTCCAG
    TCGCCAGGTG ATTGGATGTG TCCAGTTAAT AAAGGGGATG ATAAAAAAAA GAAAGATACA
    AACAAAGATG AAGAAGAGTG TAATGAGCCC AAAGGAGACC CGGAAATGGC ACCAGTATAC
    TTGAAGAGGC TGTTGCCTGT GTTTGCACAA ACATTTCAGC ACACTATGTT GCCTTCAATA
    AGGAAAGCCA GTCTTGCTTT AATTCGAAAA ATGATTCACT TTTGCTCTGA AGCACTGTTA
    AAAGAAGTCT GTGATTCTGA CGCTGGTCAC AATTTGCCCA CAGCACTAGT GGAAATCACT
    GCGACTGTCC TTGACCAAGA GGATGATGAT GATGGCCACT TACTAGCTTT GCAGATCATA
    AGGGACTTAG TAGATAAAGG TGGTGATATT TTTTTGGATC AGCTCGCCAG ACTTGGTGTA
    ATTAGCAAAG TGTCAGCTTT GGCAGGTCCT TCTTCTGATG ATGAGAATGA AGAGGAATCA
    AAACCAGAAA AGGAAGACGA ACCACAGGAA GACGCTAAAG AATTGCAGCA AGGCAAGCCG
    TACCATTGGA GAGACTGGTC AGTAATCAGA GGACGGGACT GCTTATATAT CTGGAGTGAT
    GCTGCAGCTT TGGAGTTGTC TAATGGCAGT AATGGATGGT TCAGATTTAT CTTGGATGGA
    AAACTTGCCA CCATGTATTC AAGTGGTAGT CCTGAAGGTG GATCGGATAG TTCAGAAAGC
    AGAAGTGAAT TCTTAGAGAA GTTACAAAGA GCCCGTGGCC AAGTAAAGCC ATCTACTTCA
    AGTCAACCTA TCCTGTCAGC ACCGGGGCCC ACTAAACTTA CCGTAGGGAA CTGGTCATTA
    ACATGTTTGA AAGAAGGAGA AATTGCTATT CATAACTCAG ATGGTCAGCA AGCTACAATA
    CTAAAGGAAG ATTTGCCTGG CTTTGTATTT GAATCTAATA GAGGAACTAA GCATTCGTTT
    ACTGCAGAAA CATCTCTGGG TTCAGAGTTT GTGACTGGCT GGACTGGCAA AAGAGGCAGG
    AAATTAAAAT CCAAATTGGA AAAAACAAAG CAAAAGGTAC GAACTATGGC CCGAGATTTG
    TATGATGACC ACTTTAAAGC TGTGGAAAGC ATGCCTCGTG GAGTGGTGGT AACACTCAGA
    AATATAGCAA CTCAGTTGGA GTCATCTTGG GAACTTCATA CGAATAGACA ATGCATCGAA
    GGTGAAAACA CATGGCGAGA CTTAATGAAG ACAGCATTAG AAAACCTGAT TGTGCTTCTG
    AAGGATGAGA GCACGATCTC CCCATATGAG ATGTGCAGCA GTGGCTTAGT CCAAGCCCTG
    CTCACTGTAT TGAACAGTAG TATAGATTTG GATATGAAGC AAGATTGCAG TCAACTGGTA
    GAAAGAATAA ATGTCTTCAA AACAGCCTTC AGTGAAAGTG AAGATGATGA AAGTAGACCA
    GCAGTTGCAT TAATTCGAAA GTTAATAGCT GTACTAGAAT CAATTGAGCG ACTACCTCTC
    CATTTGTATG ATACACCAGG ATCCACATAT AACCTCCAGA TACTGACAAG AAGATTACGC
    TTTCGGTTGG AACGTGCACC TGGTGAAACT GCATTAATAG ACCGGACAGG CAGGATGCTG
    AAGATGGAGC CCTTAGCTAC AGTTGAATCT CTGGAACAGT ATCTGCTGAA AATGGTAGCA
    AAGCAGTGGT ACGATTTTGA CCGGTCCTCA TTTGTTTTTG TTCGAAAATT GAGAGAAGGC
    CAAAATTTTA TATTTCGGCA CCAGCATGAT TTTGATGAAA ATGGAATCAT TTACTGGATT
    GGAACAAATG CAAAAACTGC ATATGAATGG GTAAATCCTG CAGCTTATGG TCTTGTAGTC
    GTGACATCAT CAGAAGGAAG AAATCTACCA TATGGCCGCT TGGAAGACAT ACTAAGTCGA
    GATAATTCAG CTTTAAATTG TCATAGCAAT GATGATAAAA ATGCCTGGTT TGCCATAGAT
    CTGGGTGTCT GGGTAATACC ATCAGCATAC ACACTTCGTC ATGCTCGTGG CTATGGAAGG
    TCTGCACTGA GAAACTGGGT TTTCCAGGTA TCCAAAGATG GACAGAACTG GACTTCTTTG
    TATACCCATG TTGATGACTG CAGTCTCAAT GAACCAGGGT CAACTGCAAC GTGGCCACTT
    GACCCAGCAA AGGATGAAAA ACAAGGATGG CGGCATGTGA GGCTGAAGCA GATGGGAAAG
    AACGCAAGCG GACAGACACA CTACCTCTCC CTCTCTGGCT TTGAGCTCTA TGGCACTGTA
    AATGGCGTGT GTGAAGACCA ACTCGGGAAA GCAGCTAAAG AAGCAGAAGC TAATCTTAGA
    AGGCAGAGGC GCCTGGTGCG TTCTCAAGTC CTGAAGTACA TGGTTCCAGG AGCACGTGTT
    ATCAGAGGCC TTGACTGGAA GTGGCGAGAT CAGGATGGCA GCCCACAGGG AGAAGGCACA
    GTCACAGGAG AGCTGCACAA TGGCTGGATT GATGTCACCT GGGATGCTGG TGGCTCAAAC
    TCTTACCGTA TGGGCGCAGA AGGAAAATTT GACCTCAAGC TTGCACCAGG GTACGACCCT
    GATACAGTGG CATCACCCAA ACCTGTTTCA TCCACTGTTT CAGGCACAAC GCAGTCATGG
    AGCAGCTTGG TGAAAAACAA CTGTCCAGAC AAGACGTCTG CTGCTGCAGG CTCCTCAAGT
    AGAAAAGGAA GCAGCAGTTC TGTGTGTAGC GTGGCCAGTA GCAGCGACAT CAGCTTGGGT
    TCGACCAAAA CGGAACGGAG ATCAGAAATT GTAATGGAAC ACAGTATAGT TTCAGGAGCT
    GATGTCCATG AACCAATTGT TGTTCTTTCA TCTGCTGAAA ACGTTCCTCA AACAGAAGTA
    GGGTCATCTT CCAGTGCAAG CACCAGCACC CTAACAGCGG AAACGGGAAG TGAAAATGCT
    GAAAGGAAGT TAGGCCCGGA TAGTTCTGTT CGAGCTCCTG GGGAGTCTAG TGCAATATCC
    ATGGGAATTG TTAGCGTTAG CTCTCCTGAT GTTAGTTCAG TATCTGAATT AACTAATAAG
    GAAGCAGCTT CACAACGACC CCTTAGCTCG TCAGCAAGTA ACAGACTGTC AGTGAGCTCT
    CTGTTGGCAG CTGGGGCCCC TATGAGCTCT AGTGCAAGTG TACCTAACCT GTCCTCAAGA
    GAAACATCTA GCTTGGAGAG CTTTGTAAGG AGAGTGGCAA ACATAGCACG GACTAATGCC
    ACCAACAACA TGAACCTGAG CCGGAGCAGC AGTGATAACA ACACTAACAC TCTGGGGCGG
    AATGTGATGA GCACCGCAAC TTCTCCTCTT ATGGGTGCTC AGAGTTTCCC TAATTTGACC
    ACACCTGGTA CTACATCGAC AGTGACAATG TCAACATCAA GTGTTACTAG CAGCAGCAAT
    GTAGCTACGG CGACAACAGT TTTATCAGTG GGTCAGTCAT TAAGTAATAC TTTAACCACC
    AGCCTCACAT CAACCTCTAG TGAGAGTGAC ACAGGGCAGG AAGCAGAATA TTCCTTATAT
    GACTTTCTTG ATAGCTGTCG TGCCAGTACT CTCTTAGCTG AGCTAGATGA TGACGAGGAC
    TTGCCTGAAC CGGATGAGGA AGACGATGAG AACGAAGATG ACAATCAGGA GGACCAAGAA
    TACGAGGAGG TTATGATTCT GAGACGTCCA TCTTTGCAAC GTCGTGCTGG CTCTCGCTCT
    GATGTAACAC ACCATGCTGT CACCTCACAG CTACCACAGG TCCCTTCTGG AGCAGGGAGC
    CGACCTATTG GGGAACAGGA AGAAGAAGAG TATGAAACTA AAGGAGGCCG CCGGAGAACC
    TGGGATGACG ACTACGTGCT GAAGCGGCAG TTCTCTGCAC TGGTTCCCGC TTTTGATCCT
    AGGCCTGGTC GTACCAATGT CCAACAGACA ACTGACCTAG AAATCCCTCC CCCAGGAACA
    CCTCACTCAG AGCTCTTGGA GGAAGTCGAG TGTACTCCAT CACCTCGATT GGCTCTCACG
    TTGAAAGTGA CTGGGCTTGG AACGACTCGG GAAGTTGAAC TGCCACTTAG CAACTTCAGA
    TCAACCATCT TTTATTATGT ACAGAAACTG CTTCAGCTGT CTTGTAATGG CAATGTGAAG
    TCAGACAAAC TTAGGCGTAT TTGGGAGCCA ACTTACACAA TCATGTATAG AGAAATGAAG
    GATTCTGATA AAGAAAAGGA AAATGGGAAA ATGGATTTTT GTGAGCATGG AACAAGGTCT
    GGATTAAACC AGGGGGCTAG TTCTAGTCTT CAAAGCAGTG ATATTCTTAA TTTGACAAAA
    GAGCAGCCTC AGGCTAAAGC CGGCAACGGA CAGAACCCCT GTGGAGTAGA GGACGTTCTT
    CAGCTTCTGC GCATTCTGTA TATAGTTGCA AGTGACCCTT ATTCAAGGAT ATCCCAAGAA
    GATGGTGATG AGCAGCCCCA GTTTACTTTT CCACCTGATG AATTTACTAG TAAAAAGATC
    ACCACAAAAA TTTTGCAGCA GATTGAGGAG CCATTGGCTT TGGCAAGCGG GGCTCTGCCA
    GACTGGTGTG AACAGCTGAC CAGCAAGTGT CCTTTCCTAA TCCCATTCGA AACCAGACAG
    CTTTACTTCA CCTGTACTGC GTTTGGTGCA TCCAGAGCAA TAGTGTGGCT ACAGAATCGA
    CGGGAAGCCA CAGTGGAGCG AACAAGGACC ACGAGCAGTG TAAGGCGAGA TGATCCTGGG
    GAATTTAGAG TTGGCCGACT CAAGCACGAA AGAGTGAAGG TTCCCCGTGG AGAGTCTCTG
    ATGGAGTGGG CTGAGAATGT CATGCAAATA CACGCAGACC GGAAGTCAGT TCTCGAGGTT
    GAATTTCTAG GAGAAGAAGG AACTGGCCTG GGACCCACTT TAGAGTTTTA TGCTCTTGTG
    GCAGCAGAAT TCCAGAGGAC CGACTTGGGG ACTTGGCTTT GTGATGATAA CTTTCCAGAT
    GATGAATCTC GTCATGTTGA CCTTGGAGGT GGGTTGAAGC CTCCTGGATA CTATGTACAG
    CGATCATGTG GACTGTTCAC AGCGCCATTT CCACAAGACA GTGATGAGCT GGAAAGGATC
    ACAAAACTCT TCCATTTCCT TGGGATTTTC TTGGCCAAAT GTATTCAAGA CAATAGATTG
    GTGGACTTGC CTATTTCTAA GCCTTTCTTT AAACTTATGT GCATGGGCGA CATTAAAAGC
    AATATGAGCA AACTGATCTA TGAATCACGA GGCGACAGAG ACTTGCACTG CACTGAAAGT
    CAGTCTGAAG CGTCTACGGA AGAAGGTCAT GACTCCCTCT CAGTAGGAAG CTTTGAAGAG
    GATTCAAAAT CAGAATTTAT CCTTGATCCC CCTAAGCCCA AACCCCCGGC TTGGTTTAAT
    GGAATTTTAA CTTGGGAAGA TTTTGAACTA GTAAATCCAC ACAGAGCCAG ATTTTTAAAA
    GAAATTAAAG ACCTTGCTAT TAAGAGGCGC CAGATTTTAG GCAATAAAAG CCTTTCCGAA
    GATGAGAAGA ACACAAAGTT ACAGGAACTA GTACTCAAGA ACCCATCAGG TTCTGGACCT
    CCACTTAGCA TAGAGGACTT AGGCTTAAAT TTCCAGTTTT GCCCATCTTC CAGAATATAT
    GGCTTCACAG CGGTGGACCT CAAGCCAAGT GGAGAAGATG AGATGATAAC AATGGATAAT
    GCAGAAGAAT ATGTGGATTT GATGTTTGAC TTTTGTATGC ATACGGGTAT CCAGAAACAG
    ATGGAAGCTT TTCGAGATGG GTTTAATAAA GTTTTTCCAA TGGAGAAATT AAGTTCCTTC
    AGCCATGAGG AAGTCCAGAT GATTCTTTGT GGAAATCAAT CACCATCCTG GGCAGCAGAG
    GACATTATCA ATTATACTGA ACCTAAGCTT GGCTATACAC GTGACAGTCC CGGCTTCCTT
    AGGTTTGTGA GGGTCTTATG TGGCATGTCC TCAGACGAAC GGAAAGCATT CCTGCAGTTT
    ACCACCGGCT GTTCAACTCT GCCCCCAGGT GGACTGGCTA ACCTGCATCC GAGGCTCACA
    GTCGTGCGAA AGGTTGATGC TACTGATGCA AGCTATCCAT CCGTCAACAC TTGTGTCCAC
    TATCTTAAGT TACCTGAATA TTCTTCTGAG GAGATCATGA GAGAGCGCTT GCTAGCTGCT
    ACAATGGAGA AAGGCTTTCA TCTCAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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